id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260048-yis26g81	McNamara, Ryan P.	High-density amplicon sequencing identifies community spread and ongoing evolution of SARS-CoV-2 in the Southern United States	2020-10-20		.txt	text/plain	2272	156	55	The 154 number of reads aligned varied depending on the viral load, as determined by real-time qPCR using 155 CDC primer N1, but not total RNA, as determined using RNAse P, of the samples ( Figure 1B) . At a CP ≥35 most positive samples still yielded reads that mapped to the target genome 158 and thus allowed detection of SARS-CoV-2 sequences; however, the results were less consistent, 159 and coverage was more variable. This data is consistent with the 187 astonishingly high reported genome copy numbers of SARS-CoV-2 in some cases 188 and demonstrates the principal suitability of "testing by sequencing" as a diagnostic option for SARSCoV-2 and other rapidly evolving viruses. In sum, this study generated exhaustive SNV information representing the introduction and 276 spread of SARS-CoV-2 across a suburban low-density area in the Southern U.S. All samples were 277 from symptomatic cases and the majority of genomes clustered with variants that predominate the 278 outbreak in the U.S., rather than Europe or China.	./cache/cord-260048-yis26g81.txt	./txt/cord-260048-yis26g81.txt