id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259238-n2uuaof6	Zhang, Bao-Zhong	SARS-CoV-2 infects human neural progenitor cells and brain organoids	2020-08-04		.txt	text/plain	1887	136	51	To explore the direct involvement of SARS-CoV-2 in the CNS in physiologically relevant models, we assessed SARS-CoV-2 infection in induced pluripotent stem cells (iPSCs)-derived human neural progenitor cells (hNPCs), neurospheres, and brain organoids. Importantly, extensive SARS-CoV-2 antigen was detected in the infected samples at 72 hpi (Fig. 1f) , indicating that SARS-CoV-2 directly infected the brain organoids. The results demonstrated SARS-CoV-2 RdRp gene copy number increased in a timedependent manner, suggesting active release of progeny virus particles from infected brain organoids (Fig. 1g, left) . Plaque assays performed on supernatant samples from brain organoids infected with SARS-CoV-2 showed that the infectious virus titer peaked at 24 hpi and were continuously detected at 48 and 72 hpi. h Representative images of SARS-CoV-2-infected human brain organoids immunostained for SARS-CoV-2-N and TUJ1. i Representative images of SARS-CoV-2-infected human brain organoids immunostained for SARS-CoV-2-N and NESTIN.	./cache/cord-259238-n2uuaof6.txt	./txt/cord-259238-n2uuaof6.txt