id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258624-041cf99j	Ahmad, Sajjad	Design of a Novel Multi Epitope-Based Vaccine for Pandemic Coronavirus Disease (COVID-19) by Vaccinomics and Probable Prevention Strategy against Avenging Zoonotics	2020-05-23		.txt	text/plain	8187	434	48	title: Design of a Novel Multi Epitope-Based Vaccine for Pandemic Coronavirus Disease (COVID-19) by Vaccinomics and Probable Prevention Strategy against Avenging Zoonotics We identified non-structural protein 8 (Nsp8), 3C-like proteinase, and spike glycoprotein as potential targets for immune responses to COVID-19. In order to estimate the MMPBSA binding free energies for the receptors and multi-epitope peptide vaccine construct, the MMPBSA.py module [56] of AMBER16 was castoff. The B-cell epitopes predicted for the vaccine candidates were in the following order: nine for Nsp8 and 3C-like proteinase, five for Nsp9, eight for Nsp10, 34 for spike glycoprotein and surface glycoprotein, and four for ORF1ab polyprotein| partial. Molecular interactions and binding conformation of the designed MEPVC with TLR3 and TLR4 innate immune receptors were deciphered via a protein-peptide docking approach. The dynamic simulations of the human immune system in response to the designed vaccine construct were deciphered through C-immsim server [40] .	./cache/cord-258624-041cf99j.txt	./txt/cord-258624-041cf99j.txt