id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258167-jqm3qyfm	Zhou, Peng	Immunogenicity difference between the SARS coronavirus and the bat SARS-like coronavirus spike (S) proteins	2009-09-18		.txt	text/plain	2309	118	57	Studies using wild bat sera revealed that it is highly likely that the immunodominant epitopes overlap with the major neutralizing sites of the SL-CoV S protein. Our previous studies showed that the SL-CoV and SARS-CoV shared similar genomic organization and highly conserved gene products, with the exception of the spike protein (S protein), which had a low sequence identity, especially in the receptor binding domain (RBD) located at the N-terminal region of the S proteins. In this study, the immunogenicity and immunodominant region of S SL was determined using sera generated from DNA immunization and naturally infected bats. Hyperimmune mouse sera were generated by DNA immunizations with plasmids pcDNA 3.1(+) containing the codon-optimized fulllength S gene of SARS-CoV BJ01, SL-CoV Rp3, and two chimeric plasmids CS 310-518 and CS 259-518 (see Fig. 1 for diagrams). In this study, DNA constructs expressing four different S proteins, SARS-CoV BJ01, SL-CoV Rp3 S and two chimeras, were used to generate hyperimmune sera in mice via DNA immunization.	./cache/cord-258167-jqm3qyfm.txt	./txt/cord-258167-jqm3qyfm.txt