id author title date pages extension mime words sentences flesch summary cache txt cord-255178-mb784dam Velu, P. Rapid implementation of SARS-CoV-2 emergency use authorization RT-PCR testing and experience at an academic medical institution 2020-06-08 .txt text/plain 2351 159 60 NP and sputum samples were tested on the altona RealStar® rRT-PCR 145 assay to ensure the absence of SARS-CoV-2 and pooled for use as a matrix for spiking 146 in RNA for LOD studies and accuracy studies. Probit analysis was applied to the NP data after an additional five replicates 187 of testing were performed at 0.8, 0.6, 0.5, 0.4, and 0.2 gene copies/reaction, and 188 narrowed the LOD to 2.7 gene copies/reaction at 95% detection rate (Figure 2) The in silico analysis for inclusivity that was performed by the manufacturer of the kit 197 found 100% homology of the E gene and S gene forward and reverse primers and probes 198 with 563 whole-genome sequences of SARS-CoV-2 published in GISAID and NCBI as of 199 3/16/2020 [9] . ./cache/cord-255178-mb784dam.txt ./txt/cord-255178-mb784dam.txt