id author title date pages extension mime words sentences flesch summary cache txt cord-255170-bp3irxlh Mark, John SARS coronavirus: Unusual lability of the nucleocapsid protein 2008-12-12 .txt text/plain 5274 355 95 The exper i ments used prep a ra tions of recombinant N-pro tein from which the His-tag had not been enzy mat i cally removed; thus, cal cu la tion of the resul tant pro tein/ pep tide masses must take into con sid er ation the pres ence of the His-tag (an addi tional 11 amino acid sequence). A spe cific pro te ol y sis assay was then devel oped using pep tides con tain ing the SR-rich region cor re spond ing to the SARS N-pro tein cleav age site. Taken together, the results from the FITC-labelled casein, the refold ing, and the FRET (EDANS/DAB CYL) detec tion exper i ments strongly sug gest that the SR-spe cific cleav age of N-pro tein is not the result of a con tam i nat ing pro te ase activ ity. Cell type-spe cific cleav age of nucle o cap sid pro tein by effec tor casp as es dur ing SARS coro na vi rus infec tion ./cache/cord-255170-bp3irxlh.txt ./txt/cord-255170-bp3irxlh.txt