id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-254900-fg5wd0nh	Havenga, M.J.E.	Serum‐free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivity	2007-12-13		.txt	text/plain	7456	339	48	Because of these features it can be envisioned that the use of adenoviral vectors results in higher protein yields derived from mammalian cells cultured in suspension as compared to current DNA transfection protocols for which processes at scale like cell uptake and nuclear localization, present major technical hurdles. Based on the data obtained thus far it was concluded that an DE1/DE2A adenoviral vector can be efficiently produced at 348C on PER.E2A cells and can subsequently serve as a tranducing agent for PER.C6 cells without inducing viral backbone replication or high level viral capsid protein expression in human A549 indicator cells. As shown in Figure 2C the yield of SARS anti-body produced did not significantly differ demonstrating that cleared vector stock directly harvested from he PER.E2A cell line can be used for production of recombinant protein without compromising yield.	./cache/cord-254900-fg5wd0nh.txt	./txt/cord-254900-fg5wd0nh.txt