id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-252033-43fbfglt	Plebani, Mario	Diagnostic performances and thresholds: the key to harmonization in serological SARS-CoV-2 assays?	2020-05-30		.txt	text/plain	2999	152	44	METHODS: Sera from a total of 271 subjects, including 64 reverse transcription-polymerase chain reaction (RT-PCR) confirmed SARS-CoV-2 patients were tested for specific Ab using Maglumi (Snibe), Liaison (Diasorin), iFlash (Yhlo), Euroimmun (Medizinische Labordiagnostika AG) and Wantai (Wantai Biological Pharmacy) assays. Aim of this study was to evaluate different chemiluminescent (CLIA) and enzyme-linked immunosorbent (ELISA) assays for SARS-CoV-2 antibodies in COVID-19 patients and healthcare operators, to identify appropriate cut-offs and evaluate diagnostic accuracy. Donors (only for CLIA assays) and negative autoimmune patients results were included to verify possible analytical interferences and differences with respect to healthcare workers who repeatedly tested negative to nasopharyngeal swab. Rigorous comparative performance data are crucial to understanding the potential clinical usefulness of serological assays, starting from the evaluation of analytical performance characteristics to improve the definition of diagnostic accuracy not only in terms of specificity and sensitivity but also as positive and negative likelihood ratios, in order to provide reliable clinical information in different disease prevalence settings.	./cache/cord-252033-43fbfglt.txt	./txt/cord-252033-43fbfglt.txt