id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-252019-tbalg6k5	Barra, Gustavo Barcelos	Analytical Sensitivity and Specificity of Two RT-qPCR Protocols for SARS-CoV-2 Detection Performed in an Automated Workflow	2020-10-12		.txt	text/plain	4900	268	51	Here, the following analytical performance characteristics of Charité and CDC protocols for SARS-CoV-2 detection were evaluated: (a) analytical specificity, which refers to the qPCR assay detecting the appropriate target sequence rather than other nonspecific targets also present in a sample [17] ; (b) PCR amplification efficiency, which is the increase in amplicon per cycle, and is highly dependent on the primers used [17, 18] ; (c) analytical sensitivity or limit of detection, which refers to the minimum number of nucleic acid copies in a sample that can be detected with 95% probability [17] ; (d) cross-reactivity with other pathogens; (e) on-going accuracy in clinical specimens, which refers to agreement between the test method and another method during the daily routine.	./cache/cord-252019-tbalg6k5.txt	./txt/cord-252019-tbalg6k5.txt