id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-033951-77tfhm5b	Ma, Chunlong	Ebselen, Disulfiram, Carmofur, PX-12, Tideglusib, and Shikonin Are Nonspecific Promiscuous SARS-CoV-2 Main Protease Inhibitors	2020-10-09		.txt	text/plain	6998	428	60	In this study, we investigated the mechanism of action of six previously reported M(pro) inhibitors, ebselen, disulfiram, tideglusib, carmofur, shikonin, and PX-12, using a consortium of techniques including FRET-based enzymatic assay, thermal shift assay, native mass spectrometry, cellular antiviral assays, and molecular dynamics simulations. 31 Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin were recently reported as SARS-CoV-2 M pro inhibitors with IC 50 values ranging from 0.67 to 21.39 μM in the FRET-based enzymatic assay. Collectively, our results showed that in the absence of DTT, ebselen, disulfiram, tideglusib, carmofur, shikonin, and PX-12 nonspecifically inhibit all six viral cysteine proteases including SARS-CoV-2 M pro . Collectively, the enzymatic assay results suggest that ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are promiscuous cysteine protease inhibitors that inhibit not only M pro but also five other related and unrelated viral cysteine proteases including SARS-CoV-2 PL pro and EV-A71 and EV-D68 2A pro and 3C pro in the absence of DTT, and the 	./cache/cord-033951-77tfhm5b.txt	./txt/cord-033951-77tfhm5b.txt