key: cord-354372-vfvnjmv1
authors: Carpenito, L.; D'Ercole, M.; Porta, F.; Di Blasi, E.; Doi, P.; Fagara, G. Redolfi; Rey, R.; Bulfamante, G.
title: The autopsy at the time of SARS-CoV-2: Protocol and lessons
date: 2020-07-04
journal: Ann Diagn Pathol
DOI: 10.1016/j.anndiagpath.2020.151562
sha: 
doc_id: 354372
cord_uid: vfvnjmv1

A new viral disease named COVID-19 has recently turned into a pandemic. Compared to a common viral pneumonia it may evolve in an atypical way, causing the rapid death of the patient. For over two centuries, autopsy has been recognized as a fundamental diagnostic technique, particularly for new or little-known diseases. To date, it is often considered obsolete giving the inadequacy to provide samples of a quality appropriate to the sophisticated diagnostic techniques available today. This is probably one of the reasons why during this pandemic autopsies were often requested only in few cases, late and discouraged, if not prohibited, by more than one nation. This is in contrast with our firm conviction: to understand the unknown we must look at it directly and with our own eyes. This has led us to implement an autopsy procedure that allows the beginning of the autopsy shortly after death (within 1–2 h) and its rapid execution, also including sampling for ultrastructural and molecular investigations. In our experience, the tissue sample collected for diagnosis and research were of quality similar to biopsy or surgical resections. This procedure was performed ensuring staff and environmental safety. We want to propose our experience, our main qualitative results and a few general considerations, hoping that they can be an incentive to use autopsy with a new procedure adjusted to match the diagnostic challenges of the third millennium.

The autopsy has played an essential role in the classification and definition of the etiology and pathogenesis of diseases for over two centuries, since the pivotal work "De sedibus et causis morborum per anatomen indagatis" of G.B. Morgagni (Venezia, 1761). This role is still relevant, given the periodic appearance of new nosological entities and the resulting need to understand their characteristics, consequences on the human body and possible pharmacological treatment. The World Health Organization (WHO) reports that new viral diseases and consequent epidemics will continue to appear with relevant consequences for public health. When a new infectious and diffusive disease appears the autopsy provides, other than the usual clinical and scientific data, crucial epidemiological information useful for the proper planning of hygiene and public health programs and contributes also to the correct address of health care expenditure. The autopsy ascertains the cause of death both in hospitalized patients and in people who died without medical assistance, transported to hospital or in a morgue. Last, but not least, the autopsy is a valid aid for training and education of clinicians themselves [1] [2] [3] . In recent months we have witnessed the emergency of a new global threat, the SARS-CoV-2, which produced a new disease that quickly turned into a pandemic. Its epidemiology, pathogenesis and, therefore, therapeutic possibilities are still little known [4] . In the current pandemic scenario of SARS-CoV-2, the autopsy appears to be a crucial tool to clarify the virus target cells in human, the frameworks of organ damage and the biological mechanisms that lead to death or allow the patient to heal. The autopsy execution on a patient who died of SARS-CoV-2 meets two conflicting needs. First, the high infectivity and dangerousness of the virus that requires the adoption of rigorous but timeconsuming methods to guarantee the safety of the personnel carrying out the investigation and to prevent the spread of the virus outside the autopsy room. On the other hand, the need to carry out the autopsy as soon as possible after death and to perform it quickly, in order to have as little tissue damage as possible from post-mortem degenerative phenomena [5] . The quality of the samples is J o u r n a l P r e -p r o o f essential for diagnostic and research activities, necessary to improve the standard of health care [6] [7] [8] . The practical challenge with the SARS-Cov-2 emergency led us to significantly change our operational autopsy protocol, to obtain qualitative technical results that go beyond the limits of this disease and that can be useful in a much wider range of situations. This article reports our modus operandi and the main qualitative results obtained and discusses the findings and the horizons of autopsy in the third millennium.

We performed ten autopsies on SARS-CoV-2 positive patients. The major aims that guided us are the following: 1. minimize the risks for the personnel who performed the autopsy; 2. reduce the probability of spreading the virus into the environment through leakage of unfiltered air from the anatomical and surrounding rooms or through blood or other biological liquids; 3. obtain samples for diagnosis and research of quality similar to biopsy or surgical samples; 4. make a concrete operative contribution for doctors who followed the patients in the hospitalization wards; 5. give precise informations on causes of death to the relatives of the deceased.

The regulatory references framework in which we developed and applied our protocol was issued by the Italian Government's Ministry of Health and by the Governor of the Lombardy Region [9] [10] . Our hospital is equipped with a Complex Unit (CU) of Infectious Diseases. The autopsy room and the arrival/sampling/inclusion areas of the biological samples of the C.U. of Pathological Anatomy are designed with a safety level three (BSL3, according to CDC) [11] .

The patient's cardiac death is immediately confirmed by continuous electrocardiographic monitoring that verifies the costant absence of cardiac activity for no less than 20 minutes [12] . This procedure is almost never done in Italy; it is preferred to wait at least 16 hours after the presumed death when the first putrefactive skin spots appear. If the patient died outside the hospital but was still transported to the hospital emergency room, the EPA protocol is applied only if it has passed less than one hour since the presumed death. Our protocol sets that an EPA, complete of samples for electron microscopy and molecular investigations on RNA and proteins, must start within three hours from the presumed patient's death. In our experience the effects of postmortem processes that arise after this time prevent a sufficient diagnostic quality for these types of examinations. When it is not possible to do an EPA, the autopsy is still carried out but only routine histological, immunohistochemical and molecular investigations on DNA are performed.

After the instrumental ascertainment of death, the patient is transferred to the autopsy room. While authorization procedures for the autopsy are in progress, the doctor in charge of the deceased patient and the director of Pathological Anatomy discuss the clinical aspects of the case and any question J o u r n a l P r e -p r o o f that the autopsy must answer. The goal of this type of autopsy is not only the recognition of the cause of death or of pathologies already described in medical literature, but also the understanding of the etiopathogenetic mechanisms of this new disease and the confirmation of the adequacy of the therapies in use. This is made possible by the correlation between organ and cellular pathological findings and clinical aspects (symptoms, signs, laboratory and instrumental data). The EPA is a procedure that needs to be meticulously planned before it begins, particularly if it is performed on a patient with a potential high risk of infectivity. Nothing should be left to chance and, once the staff has entered the autopsy room, there must be no unforeseen incidents. The staff that performed the autopsy must be trained and tightly-knit to be able to make decisions quickly and to adapt their work to the needs of each specific case. In addition, the organs that need sampling also for special investigations must be planned first since these procedures lengthen the autopsy time.

According to the rules of the CDC, "Autopsies on decedents known or suspected to be COVID-19 cases should be conducted in Airborne Infection Isolation Rooms (AIIRs)". These types of rooms must be "at a negative pressure to surrounding areas", with a "a minimum of 6 air changes per hour (ACH) for existing structures and 12 ACH for renovated or new structures" and "have air exhausted directly outside or through a high efficiency particulate aerosol (HEPA) filter" [11] . The WHO's guidelines firmly suggest Biosafety Level 3 (BSL 3) for autopsies perdormed on patients died for SARS-CoV-2 [13] [14] . An autopsy room of BSL 3 must be organized as a surgical room: it must have two differentiated paths, one for entrance ("dirty" one) and one for exit ("clean" one). It must be equipped with adequate instruments and clothing checked and guaranteed. In addition, effective cleaning and sanitization of both the instruments, the sector tables and the areas used must be constantly planned. Personnel working in the room must have adequate personal protective equipment (PPE) [14] and tools that can guarantee a fast surgical act. A modern autopsy cannot be performed with old and blunt instruments. In the room, during the autopsy, there must be abundant availability of alcohol at 62-71 volumes to sanitize instruments and surfaces [15] , 10% buffered formalin, five containers for formalin fixed samples (three medium-size for organs of the right hemibody, left hemibody and median axis, two big-size for brain and heart taken in full), four numbered containers with glutaraldehyde for electron microscopy samples, numbered containers with 70% alcohol solution for samples for extraction of DNA, numbered containers with RNAlater solution for samples for extraction of RNA and proteins and a transport bag to convey the containers with the samples outside the autopsy room. Any type of freezing in isopentane / nitrogen liquid must be performed inside the autopsy room only if a dedicated freezer at -40 ° C / -80 ° C is not available. Cell culture should be avoided in the absence of biohazard hoods for high biological risk.

The autopsy staff must be composed of at least two subjects, maximum three (Table 1) : the first operator (the "dirty" one), the second operator (the "clean" one) and, possibly, a technician. In our case the dirty operator has always been a doctor specialized in autopsies. The technician must not replace the first operator in the evisceration and sampling of the organs because these maneuvers are essential for the correct medical diagnosis: the diagnosis is macroscopic and inspective (look, touch, smell), before histological or molecular.

The body of the patient must be positioned completely inside the sector table to prevent blood or biological liquids from leaking onto the floor. In particular, it must be taken care that the head is not at the edge of the table (often overdrawn and not aspirated) to prevent the dripping of blood from the incision of the scalp, thus producing small drops that splash in the environment. To minimize the dispersion of blood and biological fluids, it is essential to always operate in the area of the autopsy table: the viscera removed from the body must be placed either on the iron section table, placed above the patient's thighs, or in a large tray with high steel edges resting on the patient's legs, during weighing, macroscopic examination and sampling of the viscera. The autopsy begins with a careful external inspection of the body. The length of the body and its state of nutrition must be detected, as the condition of the skin; any skin lesions and, in particular, those likely to be related to SARS-CoV-2 infection, should be sampled [16] . The scalp incision is made with a single cut from one mastoid process to the other, passing through vertex. The skull opening must be immediately suspended if there is a smell of bone dust in the environment: this means that the suction is not effective and that the risk of environmental contamination is unacceptable. The evisceration of the brain, cerebellum and brainstem has to maintain their anatomical continuity. The block is weighed and samples are immediately taken for electron microscopy and for molecular investigations. After sampling for special surveys ( fig. 1 .A-C) the brain is immediately placed suspended inside the container with formalin, to prevent it from deforming by touching the bottom ( fig. 1.D-F) . If there are no specific indications, the structures of the inner ear, located inside the petrous rock of the temporal bone, and the eyes are not removed, as the dura mater corresponding to the base and cranial vault. The "Y" cut is preferred by us to the longitudinal one conducted from the chin to the pubis for the examination of the viscera of neck and trunk. The examination and evisceration of neck and trunk organs is performed with a mixture of techniques, also on the basis of the specific clinical questions of each individual case ( Table 2) . After the brain examination, heart and lungs are the organs most frequently evaluated and extensively sampled. A swab for molecular detection (PCR) of SARS-CoV-2 is carried out, inserting the swab in large intraparenchymal bronchi or directly into the lung parenchyma. When there is clinical evidence of cardiac arhythmias or sudden death, the heart is opened, dissected only in its lower third and fully placed in formalin to allow the study of its conduction system [17] . Cavities or blood vessels must be inspected for thrombus or clots that must be taken and measured. The next step is the evisceration and examination of liver and gallbladder in a single block; the liver parenchyma should be examined macroscopically with particular attention to the characteristics of the blood vessels contained in it. Then spleen, kidneys and adrenal glands are individually eviscerated, mobilizing the segments of the gastrointestinal tract that cover them, incising the retroperitoneal soft tissues that surround some of them and dissecting the hilar structures. These organs should also be examined macroscopically, measured and weighed. The epiploon cavity is opened and the pancreas is inspected: in the absence of focal lesions a full-thickness section of the body, approximately 5 cm in length, is removed, internally examined and sampled. In males, gonads are removed by herniating them in the abdominal cavity through the inguinal canal by traction on the spermatic cord. It is advisable to always carry out samples for electron microscopy and molecular investigations of brain, heart (right and left ventricle, interventricular septum and emerging tract of the aorta and pulmonary artery), lungs, liver (right and left lobe), spleen, kidneys, skeletal muscles (2-3 different muscles), blood and bone marrow (from the first-second rib). All of this in addition to samples for histology and immunohistochemistry. If possible, examine also gonads and pancreas. Once the J o u r n a l P r e -p r o o f samples on the main parenchymatous organs have been obtained, the autopsy is completed by gutting in a single block the tongue in continuity with the viscera of the neck and the posterior mediastinum. Also these viscera must be carefully examined macroscopically, opening the hollow ones and sampling them for histological and immunohistochemical examinations. Two obstructive ligatures are performed, immediately after the Treitz ligament and about 1 cm from the ileocecal valve, to remove the small intestine by cutting it at the base of the mesentery; the intestine must be manually inspected along its entire length and, in the absence of focal lesions (which must be sampled for histological examination), two-three sections of a few centimeters are removed and fixed in formalin; in order to avoid the dispersion of feces, the section of the intestinal segments must be carried out by cutting the bowel between two adjacent ligatures for each section point. The large intestine is then mobilized by detaching it from the abdominal wall but without removing it. The course of ureters, bladder and, in females, uterus are inspected. If there are no focal lesions, they can be sampled in situ for histological and immunohistochemical analysis, but it is advisable not to open the bladder to avoid the spread of urine. If prostate samples must be performed, the bladder has to be opened and emptied by aspirating the content. After these procedures, the abdominal tract of the aorta and the large retroperitoneal vessels are inspected. Finally, the stomach is opened in situ to examine, and possibly sample, the mucous surface.

In our experience the rigorous application of the protocol described for the execution of complete autopsies did not produce accidents or negative consequences on the staff. To date, there have not been any case of SARS-CoV-2 infection in the staff assigned to the autopsy activity (detected with real-time PCR on nasopharingeal swab, serological tests for specific antibodies for SARS-CoV-2 and clinical evaluation). The instrumental assessment of cardiac death in patients allowed the rapid execution of the autopsy and the collection of samples for histopathological, ultrastructural and biomolecular tests. We thus obtained visceral samples of the highest quality, comparable to those of biopsy and surgical resections ( fig. 2, 4 .A-C). The comparison with the autopsy samples collected after 16 or more hours clearly demonstrates how the latter are affected by marked post-mortem artifacts, which can distort the correct interpretation of the morphological pictures observed. In addition, immunohistochemical tests on autopsy samples taken within 1-2 hours from death are more reliable, which is particularly important when these tests are used to describe a new pathologic entity such as COVID-19 ( fig. 3) . Immunohistochemistry, like biomolecular investigations performed on autopsy tissue samples, allows also to detect the presence of the SARS-CoV-2 in the body, detailing its distribution in single cell types ( fig. 4.D) . The rapid collection of samples for ultrastructural examinations gives the possibility to define, with a high degree of certainty, the damaging action of the pathology in progress differentiating it from post-mortem tissue and cellular changes ( fig. 5 ).

General guidance on how to perform an autopsy on a patient suspected or infected with SARS-CoV-2 have been recently published [11] ; some autopsy studies on patients with this disease reported the observed histopathological patterns, in particular affecting the lung [18] [19] [20] [21] . In light of these indications, it seems useful to underline some practical aspects concerning the execution of J o u r n a l P r e -p r o o f autopsies in the countries where this virus is still widely spread and often undiagnosed. It is essential to consider as "potentially positive" also the patients not diagnosed with SARS-CoV-2, because maybe asymptomatic or not included in health surveillance programmes: autopsy on these subjects must be performed with the protocol described for patients definitely infected. All autopsies should always be considered "potentially at risk of contagiousness". In each hospital a specific operating protocol that allows to quickly ascertain the patient's death must be planned out so that the autopsy can be performed rapidly without waiting for the appearance of explicit putrefactive phenomena, which is the normal procedure in Italy [8] . The study of tissues markedly modified by postmortem biological phenomena can allow us to contemplate death but not the causes and the mechanisms that produced it. In our experience the histopathological and ultrastructural frameworks that emerge from an autopsy performed within 1-2 hours after death differ significantly from those that we observed in an autopsy performed many hours or days after death and which have been presented in multiple autopsy studies on SARS-CoV-2 [18] [19] [20] [21] . The autopsy must be carried out quickly and planned before executing it, adapting its performance and methodology to the specific items of each case. These must be defined by the previous interview between the doctors and the pathologist. The maneuvers useful for macroscopically examining and sampling the organs identified as the main target in every single autopsy, will be privileged. This type of autopsy is not the occasion for elegant anatomical dissections, nor for the application of traditional teaching methods on evisceration procedures. It is essential to do it early and well, aiming for the planned goals, which may change from case to case. During the collection of material for ultrastructural, molecular or other tests, it is important to be aware that not all viscera can be sampled quickly, because the autopsy has its own procedure time and the more special samples are taken, the more time will increase. Before starting the autopsy, the evisceration and sampling sequence must be planned (especially when it is necessary to take tissue for electron microscopy) and be aware that the organs sampled after one hour from the beginning of the autopsy will only be able to provide samples for histology and immunohistochemistry. In our experience the patients who undergo autopsy within few hours of death, bleed much more and the incision of very congested vessels can produce relevant blood splatters than in an autopsy performed after 24 hours or more. In addition, patients hospitalized in intensive care for SARS-CoV-2 can be anticoagulated, a condition that increases the leakage of blood. During the autopsy the blood must absolutely not be dispersed in the environment surrounding the sector table. For this reason, the body must be positioned completely inside the sector table and the mobilization of large visceral masses outside the body should be avoided: Letulle's technique (evisceration en masse) therefore does not appear adequate. Blood, urine and other biological liquids must remain inside the body or aspirated by a vacuum pump in a container that can be sanitized after the autopsy. The commonly recommended use of rags or sponges to collect blood is totally inadequate and dangerous. Given the multiple clinical findings of neurological symptoms in patients infected with SARS-CoV-2 [22, 23] , it appears indispensable to perform the evisceration and examination of the brain and brainstem, for the completeness of the autopsy and for the very few morphological data available today on the central nervous system. Our experience shows that the use of a valid circular saw with dust extraction system, combined with adequate PPE, does not put operators at risk and allows the doctor to obtain valid tissue samples. Given the importance of time, it seems useless to waste it performing the swabs for the detection of SARS-CoV-2 in the usual locations for the living, when abundant material can be collected by inserting the swab directly into the lung parenchyma.

To date we are conducting further investigations on the samples obtained from early performed autopsies in order to evaluate the organ damage, particularly on SNC, heart and lung.

At the beginning of the third millennium, it is anachronistic to engage with the challenges of our discipline with the tools available to Morgagni, Malpighi, Vesalio or Virchow, when we have new and powerful weapons at our disposal. The limited possibilities to apply modern investigation technologies to tissues severely damaged by post mortem degenerative phenomena, has led many clinicians and pathologists to believe that autopsies are obsolete although, theoretically, they may be the most complete diagnostic tool. At present the autopsy should be performed quickly after the instrumental assessment of cardiac death, to ensure that the quality of tissues examined is similar to that of biopsy or surgical samples performed on the living. The forensic autopsy is the one with the most problems because of its rapid execution is often hampered by the need to perform it in front of the consultants of the parties involved. Since it is in everyone's interest to have well-preserved tissue samples available for valid histopathological, biochemical, molecular or toxicological analyses, a possible solution could be to video-transmit or video-record the stages of the autopsy that take place at the autopsy table and that, once carried out, are not repeatable. In our opinion the SARS-CoV-2 pandemic provides two major lessons. The first is that in all the areas where the virus is still circulating, even if not epidemic, autopsies must be considered at high risk of infectivity. This should prompt to increase the number of BSL 3 autopsy rooms in order to satisfy the needs of the territories. The second lesson is that rapid autopsies for health purposes appears mandatory in every day practice, even when it is not necessary to understand the etiology and pathogenesis of a new pathology. Rapid autopsies guarantee optimal tissues to apply the most sophisticated diagnostic methodologies. This supports the classic holistic autopsy examinations, which aims not only to define the cause of death but also, and perhaps above all, to reconstruct the pathological history and style of the patient's life. In conclusion even today the autopsy does not lose its etymological meaning, crucial for the correct progress of knowledge: "see with your own eyes" (from the ancient Greek "autòs" -same-and "opsis" -sight-). This is the foundamental part of the scientific method (observe -make a hypothesis -verify the hypothesis) masterfully described by Galileo Galilei, and the first step of every medical thought process.

J o u r n a l P r e -p r o o f Table 1 

Autopsy staff -First operator (the "dirty" one): doctor, specialist in pathology, expert in performing autopsies, even in presence of diseases with high infectious risk; performs the autopsy and, if necessary, sews the body at the end. -Second operator (the "clean" one): doctor, specialist or resident in pathology, even without particular autopsy experience; takes photographs, transcribes weights, measurements, volumes and observations dictated by the first operator and assists in the collection, cataloging and storage of the samples. -Technician: supports the first operator but only if he has been trained for autopsies at high infectious risk; if present, supports the first operator and sews the body at the end. External inspection of the body -Body length, state of nutrition (maximum thickness of the adipose panniculus at the level of the chest and abdomen), skin (color, trophy and state of conservation), hair and nails, conjunctivae, sclerae, pupils, evaluation of any material that comes out from the mouth, nostrils or middle ear and, when possible, oral cavity and dentition. -Description of any pathological or morphological abnormality detectable on external examination of the body. Scalp incision and opening of the skull -Bimastoid cut of the scalp, passing through vertex of the head.

-The skull opening must be immediately suspended if there is a smell of bone dust in the environment: this fact indicates that the dust extraction system connected to the skull saw does not work. In this case the level of risk for the staff is unacceptable, due to biological microparticles free in the air. The operators must move away from the sector table for at least 20 minutes (time that guarantees at least 2 complete air exchanges in the autopsy room). Evisceration of the brain -Cerebrum, brainstem and cerebellum must be maintained in their anatomical continuity.

-Weigh the visceral block.

-Take samples for electron microscopy and molecular investigations, preferably collecting on a single hemisphere or visceral hemibody, preserving the other for histopathological investigations. -Place the residual brain in formalin, suspending it inside the container; the brain suspension is made by passing a thin string under the basilar artery and knotting its ends at the joints of the handle of the container. -Remove the pituitary gland.

"Y" cut of the skin and subcutaneous planes for the examination of neck and trunk cavities and viscera -The "Y" cut is conducted from the two acromion processes to the xiphoid process of the sternum, and from this point to the pubis along the anterior median axis of the abdomen. -Observe the characteristics of the adipose tissue, the color and trophism of skeletal muscles, the state of congestion of major blood vessels and the possible presence of pathologies or injuries. Opening of the rib cage -Assess the size of the cardiac area.

-Assess the size of the visible areas of the lungs.

-Assess the characteristics of pleural and pleural cavities.

-Measure of the maximum height of the diaphragmatic dome. Opening of the pericardial sac -Verify and quantify the presence of effusions or blood in the pericardial cavity.

-Assess the characteristics of the pericardial and epicardial surfaces -Assess the content of the pulmonary veins by incision (any thrombi or clots must be taken and measured). Opening in situ of the right chambers of the heart -Do not damage the cardiac conduction system, particularly the sinus-atrial node, the atrioventricular node and the bundle of His. -Evaluate the content of the right vessels and cardiac chambers (describing and quantifying the characteristics of any observed thrombi or clots).

J o u r n a l P r e -p r o o f -Evaluate the morphology of the atrium, tricuspid valve, ventricular chamber, ejection cone, pulmonary valve and artery and its branches. Opening in situ of the left chambers of the heart -Not open the ventricle ejection cone to avoid damaging the cardiac conduction system.

-Evaluate the content of the left vessels and cardiac chambers (describing and quantifying the characteristics of any observed thrombi or clots). -Evaluate the morphology of the atrium, mitral valve, ventricular chamber, ejection cone, pulmonary valve and artery and its branches. Eviscerate the heart -Weigh it and perform three biventricular sections from the tip towards the plane of the atrioventricular valves, on transverse planes, parallel to each other and about 1 cm apart. -On the most cranial section measure the thickness of the free walls of the two ventricles and that of the interventricular septum. -Take samples for electron microscopy and molecular investigations.

-Place the heart completely in formalin for histological and immunohistochemical studies and, where appropriate, cardiac conduction studies. Removal of the lungs by cutting them from the hilum -Weigh, macroscopically examine (external and internal parenchymal surfaces) and immediately sample, collecting all the lobes and areas with significant macroscopic differences (color, consistency, content at squeezing). Individual evisceration, weighing, sampling and examination of: -Liver and gallbladder (in a single block). Virchow technique (removal of the viscera one by one) for: lungs, spleen, kidneys, adrenals and gonads. Rokitansky's technique (opening and examination in situ of viscera and apparatus) for: heart (before removing it), thorax large blood vessels, stomach, epiploon's cavity and pancreas, duodenum, large bowel, ureters, prostate, bladder and the retroperitoneal large blood vessel. Ghon technique (evisceration en bloc) for: Brain, tongue, organs of the neck and posterior mediastinum, liver and gallbladder, small bowel. Letulle's evisceration technique (evisceration en masse) not performed to minimize the leak of blood, feces and biological fluids from the body Special notes: A. To be able to eviscerate the tongue, the sublingual salivary glands and part of the hypopharynx in continuity with the trachea, it is important that the incision of the skin and subcutaneous planes starts bilaterally from the most posterior part of the acromion and not from the anterior surface of the shoulder. B. The tongue is extracted by sectioning the frenulum and the floor below the mandibular arch after lifting the skin and subcutaneous planes of the neck and upper chest, and turning the tip over on the anterior laryngeal surface. The whole block of the tongue and viscera of the neck and the posterior mediastinum is mobilized from the vertebral plane by cutting the main vessels of the neck and dissecting the esophagus after performing an obstructive ligature in correspondence with its overdiaphragmatic tract.

To open the thoracic cage, dissect as far as possible the ribs at the level of their cartilage tract, to avoid producing bone spicules that may sting the operator; then disarticulate bilaterally the sternum-clavicular joint and thus remove the sternum and the anterior tract of the ribs. To increase the space of maneuver inside the chest, it is advisable to dissect the muscles of the intercostal spaces. D. Removal of liver-gallbladder block cutting the hilar structures, the liver ligaments, freeing the quadrate lobe and by removing the part of the phrenic muscle corresponding to the bare area of the right lobe of the liver. E. When you need to open the bladder to remove the prostate or uterus, you need to turn it; in this case, the bladder wall must be pulled upwards, making a small full-thickness hole in it from which it will be inserted a suction cannula that will empty it.

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Early performed autopsy (within 1-2 hour from death) provides tissue samples for diagnosis and research of quality similar to biopsy or surgical resections

Early samples collection reduces post-mortem artifacts, thus preventing the wrong interpretation of the morphological pictures observed

Precise autopsy planning prevents risks for the staff