id sid tid token lemma pos cord-258014-lzzi4rnz 1 1 key key JJ cord-258014-lzzi4rnz 2 1 : : : cord-258014-lzzi4rnz 2 2 cord-258014-lzzi4rnz cord-258014-lzzi4rnz JJ cord-258014-lzzi4rnz 2 3 authors author NNS cord-258014-lzzi4rnz 2 4 : : : cord-258014-lzzi4rnz 2 5 Chorna Chorna NNP cord-258014-lzzi4rnz 2 6 , , , cord-258014-lzzi4rnz 2 7 Nataliya Nataliya NNP cord-258014-lzzi4rnz 2 8 ; ; : cord-258014-lzzi4rnz 2 9 Godoy Godoy NNP cord-258014-lzzi4rnz 2 10 - - HYPH cord-258014-lzzi4rnz 2 11 Vitorino Vitorino NNP cord-258014-lzzi4rnz 2 12 , , , cord-258014-lzzi4rnz 2 13 Filipa Filipa NNP cord-258014-lzzi4rnz 2 14 title title NN cord-258014-lzzi4rnz 2 15 : : : cord-258014-lzzi4rnz 3 1 A a DT cord-258014-lzzi4rnz 3 2 Protocol Protocol NNP cord-258014-lzzi4rnz 3 3 for for IN cord-258014-lzzi4rnz 3 4 the the DT cord-258014-lzzi4rnz 3 5 Multi Multi NNP cord-258014-lzzi4rnz 3 6 - - HYPH cord-258014-lzzi4rnz 3 7 Omic Omic NNP cord-258014-lzzi4rnz 3 8 Integration Integration NNP cord-258014-lzzi4rnz 3 9 of of IN cord-258014-lzzi4rnz 3 10 Cervical Cervical NNP cord-258014-lzzi4rnz 3 11 Microbiota Microbiota NNP cord-258014-lzzi4rnz 3 12 and and CC cord-258014-lzzi4rnz 3 13 Urine Urine NNP cord-258014-lzzi4rnz 3 14 Metabolomics Metabolomics NNPS cord-258014-lzzi4rnz 3 15 to to TO cord-258014-lzzi4rnz 3 16 Understand understand VB cord-258014-lzzi4rnz 3 17 Human Human NNP cord-258014-lzzi4rnz 3 18 Papillomavirus Papillomavirus NNP cord-258014-lzzi4rnz 3 19 ( ( -LRB- cord-258014-lzzi4rnz 3 20 HPV)-Driven HPV)-Driven NNP cord-258014-lzzi4rnz 3 21 Dysbiosis Dysbiosis NNP cord-258014-lzzi4rnz 3 22 date date NN cord-258014-lzzi4rnz 3 23 : : : cord-258014-lzzi4rnz 3 24 2020 2020 CD cord-258014-lzzi4rnz 3 25 - - HYPH cord-258014-lzzi4rnz 3 26 04 04 CD cord-258014-lzzi4rnz 3 27 - - HYPH cord-258014-lzzi4rnz 3 28 08 08 CD cord-258014-lzzi4rnz 3 29 journal journal NN cord-258014-lzzi4rnz 3 30 : : : cord-258014-lzzi4rnz 4 1 Biomedicines Biomedicines NNP cord-258014-lzzi4rnz 4 2 DOI DOI NNP cord-258014-lzzi4rnz 4 3 : : : cord-258014-lzzi4rnz 5 1 10.3390 10.3390 CD cord-258014-lzzi4rnz 6 1 /biomedicines8040081 /biomedicines8040081 . cord-258014-lzzi4rnz 7 1 sha sha NNP cord-258014-lzzi4rnz 7 2 : : : cord-258014-lzzi4rnz 8 1 6190f652430d632f4d19d7fa7030bf2af12b6fae 6190f652430d632f4d19d7fa7030bf2af12b6fae CD cord-258014-lzzi4rnz 8 2 doc_id doc_id CD cord-258014-lzzi4rnz 8 3 : : : cord-258014-lzzi4rnz 8 4 258014 258014 CD cord-258014-lzzi4rnz 8 5 cord_uid cord_uid NNS cord-258014-lzzi4rnz 8 6 : : : cord-258014-lzzi4rnz 9 1 lzzi4rnz lzzi4rnz JJ cord-258014-lzzi4rnz 9 2 The the DT cord-258014-lzzi4rnz 9 3 multi multi JJ cord-258014-lzzi4rnz 9 4 - - JJ cord-258014-lzzi4rnz 9 5 omic omic JJ cord-258014-lzzi4rnz 9 6 integration integration NN cord-258014-lzzi4rnz 9 7 of of IN cord-258014-lzzi4rnz 9 8 microbiota microbiota NNP cord-258014-lzzi4rnz 9 9 data datum NNS cord-258014-lzzi4rnz 9 10 with with IN cord-258014-lzzi4rnz 9 11 metabolomics metabolomic NNS cord-258014-lzzi4rnz 9 12 has have VBZ cord-258014-lzzi4rnz 9 13 gained gain VBN cord-258014-lzzi4rnz 9 14 popularity popularity NN cord-258014-lzzi4rnz 9 15 . . . cord-258014-lzzi4rnz 10 1 This this DT cord-258014-lzzi4rnz 10 2 protocol protocol NN cord-258014-lzzi4rnz 10 3 is be VBZ cord-258014-lzzi4rnz 10 4 based base VBN cord-258014-lzzi4rnz 10 5 on on IN cord-258014-lzzi4rnz 10 6 a a DT cord-258014-lzzi4rnz 10 7 human human JJ cord-258014-lzzi4rnz 10 8 multi multi JJ cord-258014-lzzi4rnz 10 9 - - HYPH cord-258014-lzzi4rnz 10 10 omics omics JJ cord-258014-lzzi4rnz 10 11 study study NN cord-258014-lzzi4rnz 10 12 , , , cord-258014-lzzi4rnz 10 13 integrating integrate VBG cord-258014-lzzi4rnz 10 14 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 10 15 microbiota microbiota NN cord-258014-lzzi4rnz 10 16 , , , cord-258014-lzzi4rnz 10 17 HPV hpv NN cord-258014-lzzi4rnz 10 18 status status NN cord-258014-lzzi4rnz 10 19 and and CC cord-258014-lzzi4rnz 10 20 neoplasia neoplasia NN cord-258014-lzzi4rnz 10 21 , , , cord-258014-lzzi4rnz 10 22 with with IN cord-258014-lzzi4rnz 10 23 urinary urinary JJ cord-258014-lzzi4rnz 10 24 metabolites metabolite NNS cord-258014-lzzi4rnz 10 25 . . . cord-258014-lzzi4rnz 11 1 Indeed indeed RB cord-258014-lzzi4rnz 11 2 , , , cord-258014-lzzi4rnz 11 3 to to TO cord-258014-lzzi4rnz 11 4 understand understand VB cord-258014-lzzi4rnz 11 5 the the DT cord-258014-lzzi4rnz 11 6 biology biology NN cord-258014-lzzi4rnz 11 7 of of IN cord-258014-lzzi4rnz 11 8 the the DT cord-258014-lzzi4rnz 11 9 infections infection NNS cord-258014-lzzi4rnz 11 10 and and CC cord-258014-lzzi4rnz 11 11 to to TO cord-258014-lzzi4rnz 11 12 develop develop VB cord-258014-lzzi4rnz 11 13 adequate adequate JJ cord-258014-lzzi4rnz 11 14 interventions intervention NNS cord-258014-lzzi4rnz 11 15 for for IN cord-258014-lzzi4rnz 11 16 cervical cervical JJ cord-258014-lzzi4rnz 11 17 cancer cancer NN cord-258014-lzzi4rnz 11 18 prevention prevention NN cord-258014-lzzi4rnz 11 19 , , , cord-258014-lzzi4rnz 11 20 studies study NNS cord-258014-lzzi4rnz 11 21 are be VBP cord-258014-lzzi4rnz 11 22 needed need VBN cord-258014-lzzi4rnz 11 23 to to TO cord-258014-lzzi4rnz 11 24 characterize characterize VB cord-258014-lzzi4rnz 11 25 in in IN cord-258014-lzzi4rnz 11 26 detail detail NN cord-258014-lzzi4rnz 11 27 the the DT cord-258014-lzzi4rnz 11 28 cervical cervical JJ cord-258014-lzzi4rnz 11 29 microbiota microbiota NN cord-258014-lzzi4rnz 11 30 and and CC cord-258014-lzzi4rnz 11 31 understand understand VB cord-258014-lzzi4rnz 11 32 the the DT cord-258014-lzzi4rnz 11 33 systemic systemic JJ cord-258014-lzzi4rnz 11 34 metabolome metabolome NN cord-258014-lzzi4rnz 11 35 . . . cord-258014-lzzi4rnz 12 1 This this DT cord-258014-lzzi4rnz 12 2 article article NN cord-258014-lzzi4rnz 12 3 is be VBZ cord-258014-lzzi4rnz 12 4 a a DT cord-258014-lzzi4rnz 12 5 detailed detailed JJ cord-258014-lzzi4rnz 12 6 protocol protocol NN cord-258014-lzzi4rnz 12 7 for for IN cord-258014-lzzi4rnz 12 8 the the DT cord-258014-lzzi4rnz 12 9 multi multi JJ cord-258014-lzzi4rnz 12 10 - - JJ cord-258014-lzzi4rnz 12 11 omic omic JJ cord-258014-lzzi4rnz 12 12 integration integration NN cord-258014-lzzi4rnz 12 13 of of IN cord-258014-lzzi4rnz 12 14 cervical cervical JJ cord-258014-lzzi4rnz 12 15 microbiota microbiota NN cord-258014-lzzi4rnz 12 16 and and CC cord-258014-lzzi4rnz 12 17 urine urine NN cord-258014-lzzi4rnz 12 18 metabolome metabolome NN cord-258014-lzzi4rnz 12 19 to to TO cord-258014-lzzi4rnz 12 20 shed shed VB cord-258014-lzzi4rnz 12 21 light light NN cord-258014-lzzi4rnz 12 22 on on IN cord-258014-lzzi4rnz 12 23 the the DT cord-258014-lzzi4rnz 12 24 systemic systemic JJ cord-258014-lzzi4rnz 12 25 effects effect NNS cord-258014-lzzi4rnz 12 26 of of IN cord-258014-lzzi4rnz 12 27 cervical cervical JJ cord-258014-lzzi4rnz 12 28 dysbioses dysbiose NNS cord-258014-lzzi4rnz 12 29 associated associate VBN cord-258014-lzzi4rnz 12 30 with with IN cord-258014-lzzi4rnz 12 31 Human Human NNP cord-258014-lzzi4rnz 12 32 Papillomavirus Papillomavirus NNP cord-258014-lzzi4rnz 12 33 ( ( -LRB- cord-258014-lzzi4rnz 12 34 HPV HPV NNP cord-258014-lzzi4rnz 12 35 ) ) -RRB- cord-258014-lzzi4rnz 12 36 infections infection NNS cord-258014-lzzi4rnz 12 37 . . . cord-258014-lzzi4rnz 13 1 This this DT cord-258014-lzzi4rnz 13 2 methods method NNS cord-258014-lzzi4rnz 13 3 article article NN cord-258014-lzzi4rnz 13 4 suggests suggest VBZ cord-258014-lzzi4rnz 13 5 detailed detailed JJ cord-258014-lzzi4rnz 13 6 sample sample NN cord-258014-lzzi4rnz 13 7 collection collection NN cord-258014-lzzi4rnz 13 8 and and CC cord-258014-lzzi4rnz 13 9 laboratory laboratory NN cord-258014-lzzi4rnz 13 10 processes process NNS cord-258014-lzzi4rnz 13 11 of of IN cord-258014-lzzi4rnz 13 12 metabolomics metabolomic NNS cord-258014-lzzi4rnz 13 13 , , , cord-258014-lzzi4rnz 13 14 DNA dna NN cord-258014-lzzi4rnz 13 15 extraction extraction NN cord-258014-lzzi4rnz 13 16 for for IN cord-258014-lzzi4rnz 13 17 microbiota microbiota NN cord-258014-lzzi4rnz 13 18 , , , cord-258014-lzzi4rnz 13 19 HPV hpv NN cord-258014-lzzi4rnz 13 20 typing typing NN cord-258014-lzzi4rnz 13 21 , , , cord-258014-lzzi4rnz 13 22 and and CC cord-258014-lzzi4rnz 13 23 the the DT cord-258014-lzzi4rnz 13 24 bioinformatic bioinformatic JJ cord-258014-lzzi4rnz 13 25 analyses analysis NNS cord-258014-lzzi4rnz 13 26 of of IN cord-258014-lzzi4rnz 13 27 the the DT cord-258014-lzzi4rnz 13 28 data datum NNS cord-258014-lzzi4rnz 13 29 , , , cord-258014-lzzi4rnz 13 30 both both DT cord-258014-lzzi4rnz 13 31 to to TO cord-258014-lzzi4rnz 13 32 characterize characterize VB cord-258014-lzzi4rnz 13 33 the the DT cord-258014-lzzi4rnz 13 34 metabolome metabolome NN cord-258014-lzzi4rnz 13 35 , , , cord-258014-lzzi4rnz 13 36 the the DT cord-258014-lzzi4rnz 13 37 microbiota microbiota NN cord-258014-lzzi4rnz 13 38 , , , cord-258014-lzzi4rnz 13 39 and and CC cord-258014-lzzi4rnz 13 40 joint joint JJ cord-258014-lzzi4rnz 13 41 multi multi JJ cord-258014-lzzi4rnz 13 42 - - JJ cord-258014-lzzi4rnz 13 43 omic omic JJ cord-258014-lzzi4rnz 13 44 analyses analysis NNS cord-258014-lzzi4rnz 13 45 , , , cord-258014-lzzi4rnz 13 46 useful useful JJ cord-258014-lzzi4rnz 13 47 for for IN cord-258014-lzzi4rnz 13 48 the the DT cord-258014-lzzi4rnz 13 49 development development NN cord-258014-lzzi4rnz 13 50 of of IN cord-258014-lzzi4rnz 13 51 new new JJ cord-258014-lzzi4rnz 13 52 point point NN cord-258014-lzzi4rnz 13 53 - - HYPH cord-258014-lzzi4rnz 13 54 of of IN cord-258014-lzzi4rnz 13 55 - - HYPH cord-258014-lzzi4rnz 13 56 care care NN cord-258014-lzzi4rnz 13 57 diagnostic diagnostic JJ cord-258014-lzzi4rnz 13 58 tests test NNS cord-258014-lzzi4rnz 13 59 based base VBN cord-258014-lzzi4rnz 13 60 on on IN cord-258014-lzzi4rnz 13 61 these these DT cord-258014-lzzi4rnz 13 62 approaches approach NNS cord-258014-lzzi4rnz 13 63 . . . cord-258014-lzzi4rnz 14 1 A a DT cord-258014-lzzi4rnz 14 2 growing grow VBG cord-258014-lzzi4rnz 14 3 body body NN cord-258014-lzzi4rnz 14 4 of of IN cord-258014-lzzi4rnz 14 5 evidence evidence NN cord-258014-lzzi4rnz 14 6 , , , cord-258014-lzzi4rnz 14 7 especially especially RB cord-258014-lzzi4rnz 14 8 over over IN cord-258014-lzzi4rnz 14 9 the the DT cord-258014-lzzi4rnz 14 10 last last JJ cord-258014-lzzi4rnz 14 11 twelve twelve CD cord-258014-lzzi4rnz 14 12 years year NNS cord-258014-lzzi4rnz 14 13 , , , cord-258014-lzzi4rnz 14 14 suggests suggest VBZ cord-258014-lzzi4rnz 14 15 that that IN cord-258014-lzzi4rnz 14 16 the the DT cord-258014-lzzi4rnz 14 17 composition composition NN cord-258014-lzzi4rnz 14 18 and and CC cord-258014-lzzi4rnz 14 19 function function NN cord-258014-lzzi4rnz 14 20 of of IN cord-258014-lzzi4rnz 14 21 the the DT cord-258014-lzzi4rnz 14 22 microbiota microbiota NN cord-258014-lzzi4rnz 14 23 in in IN cord-258014-lzzi4rnz 14 24 different different JJ cord-258014-lzzi4rnz 14 25 human human JJ cord-258014-lzzi4rnz 14 26 body body NN cord-258014-lzzi4rnz 14 27 habitats habitat NNS cord-258014-lzzi4rnz 14 28 play play VBP cord-258014-lzzi4rnz 14 29 vital vital JJ cord-258014-lzzi4rnz 14 30 roles role NNS cord-258014-lzzi4rnz 14 31 in in IN cord-258014-lzzi4rnz 14 32 human human JJ cord-258014-lzzi4rnz 14 33 development development NN cord-258014-lzzi4rnz 14 34 and and CC cord-258014-lzzi4rnz 14 35 immunity immunity NN cord-258014-lzzi4rnz 14 36 [ [ -LRB- cord-258014-lzzi4rnz 14 37 1 1 CD cord-258014-lzzi4rnz 14 38 ] ] -RRB- cord-258014-lzzi4rnz 14 39 [ [ -LRB- cord-258014-lzzi4rnz 14 40 2 2 CD cord-258014-lzzi4rnz 14 41 ] ] -RRB- cord-258014-lzzi4rnz 14 42 [ [ -LRB- cord-258014-lzzi4rnz 14 43 3 3 CD cord-258014-lzzi4rnz 14 44 ] ] -RRB- cord-258014-lzzi4rnz 14 45 . . . cord-258014-lzzi4rnz 15 1 The the DT cord-258014-lzzi4rnz 15 2 vagina vagina NN cord-258014-lzzi4rnz 15 3 and and CC cord-258014-lzzi4rnz 15 4 cervix cervix NN cord-258014-lzzi4rnz 15 5 have have VBP cord-258014-lzzi4rnz 15 6 the the DT cord-258014-lzzi4rnz 15 7 lowest low JJS cord-258014-lzzi4rnz 15 8 biodiversity biodiversity NN cord-258014-lzzi4rnz 15 9 , , , cord-258014-lzzi4rnz 15 10 with with IN cord-258014-lzzi4rnz 15 11 a a DT cord-258014-lzzi4rnz 15 12 colonized colonize VBN cord-258014-lzzi4rnz 15 13 epithelium epithelium NN cord-258014-lzzi4rnz 15 14 dominated dominate VBN cord-258014-lzzi4rnz 15 15 by by IN cord-258014-lzzi4rnz 15 16 Lactobacilli Lactobacilli NNP cord-258014-lzzi4rnz 15 17 [ [ -LRB- cord-258014-lzzi4rnz 15 18 4 4 CD cord-258014-lzzi4rnz 15 19 ] ] -RRB- cord-258014-lzzi4rnz 15 20 , , , cord-258014-lzzi4rnz 15 21 thus thus RB cord-258014-lzzi4rnz 15 22 being be VBG cord-258014-lzzi4rnz 15 23 an an DT cord-258014-lzzi4rnz 15 24 interface interface NN cord-258014-lzzi4rnz 15 25 that that WDT cord-258014-lzzi4rnz 15 26 plays play VBZ cord-258014-lzzi4rnz 15 27 a a DT cord-258014-lzzi4rnz 15 28 protective protective JJ cord-258014-lzzi4rnz 15 29 role role NN cord-258014-lzzi4rnz 15 30 between between IN cord-258014-lzzi4rnz 15 31 the the DT cord-258014-lzzi4rnz 15 32 host host NN cord-258014-lzzi4rnz 15 33 and and CC cord-258014-lzzi4rnz 15 34 the the DT cord-258014-lzzi4rnz 15 35 environment environment NN cord-258014-lzzi4rnz 15 36 . . . cord-258014-lzzi4rnz 16 1 The the DT cord-258014-lzzi4rnz 16 2 cervix cervix NN cord-258014-lzzi4rnz 16 3 serves serve VBZ cord-258014-lzzi4rnz 16 4 a a DT cord-258014-lzzi4rnz 16 5 pivotal pivotal JJ cord-258014-lzzi4rnz 16 6 role role NN cord-258014-lzzi4rnz 16 7 as as IN cord-258014-lzzi4rnz 16 8 a a DT cord-258014-lzzi4rnz 16 9 gatekeeper gatekeeper NN cord-258014-lzzi4rnz 16 10 to to TO cord-258014-lzzi4rnz 16 11 protect protect VB cord-258014-lzzi4rnz 16 12 the the DT cord-258014-lzzi4rnz 16 13 upper upper JJ cord-258014-lzzi4rnz 16 14 genital genital JJ cord-258014-lzzi4rnz 16 15 tract tract NN cord-258014-lzzi4rnz 16 16 from from IN cord-258014-lzzi4rnz 16 17 microbial microbial JJ cord-258014-lzzi4rnz 16 18 invasion invasion NN cord-258014-lzzi4rnz 16 19 and and CC cord-258014-lzzi4rnz 16 20 subsequent subsequent JJ cord-258014-lzzi4rnz 16 21 pathology pathology NN cord-258014-lzzi4rnz 16 22 . . . cord-258014-lzzi4rnz 17 1 Recent recent JJ cord-258014-lzzi4rnz 17 2 studies study NNS cord-258014-lzzi4rnz 17 3 have have VBP cord-258014-lzzi4rnz 17 4 indicated indicate VBN cord-258014-lzzi4rnz 17 5 that that IN cord-258014-lzzi4rnz 17 6 changes change NNS cord-258014-lzzi4rnz 17 7 of of IN cord-258014-lzzi4rnz 17 8 the the DT cord-258014-lzzi4rnz 17 9 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 17 10 microbiome microbiome NNP cord-258014-lzzi4rnz 17 11 [ [ -LRB- cord-258014-lzzi4rnz 17 12 5 5 CD cord-258014-lzzi4rnz 17 13 ] ] -RRB- cord-258014-lzzi4rnz 17 14 [ [ -LRB- cord-258014-lzzi4rnz 17 15 6 6 CD cord-258014-lzzi4rnz 17 16 ] ] -RRB- cord-258014-lzzi4rnz 17 17 [ [ -LRB- cord-258014-lzzi4rnz 17 18 7 7 CD cord-258014-lzzi4rnz 17 19 ] ] -RRB- cord-258014-lzzi4rnz 17 20 , , , cord-258014-lzzi4rnz 17 21 such such JJ cord-258014-lzzi4rnz 17 22 as as IN cord-258014-lzzi4rnz 17 23 bacterial bacterial JJ cord-258014-lzzi4rnz 17 24 vaginosis vaginosis NN cord-258014-lzzi4rnz 17 25 [ [ -LRB- cord-258014-lzzi4rnz 17 26 8 8 CD cord-258014-lzzi4rnz 17 27 , , , cord-258014-lzzi4rnz 17 28 9 9 CD cord-258014-lzzi4rnz 17 29 ] ] -RRB- cord-258014-lzzi4rnz 17 30 , , , cord-258014-lzzi4rnz 17 31 cervical cervical JJ cord-258014-lzzi4rnz 17 32 inflammation inflammation NN cord-258014-lzzi4rnz 17 33 [ [ -LRB- cord-258014-lzzi4rnz 17 34 10 10 CD cord-258014-lzzi4rnz 17 35 , , , cord-258014-lzzi4rnz 17 36 11 11 CD cord-258014-lzzi4rnz 17 37 ] ] -RRB- cord-258014-lzzi4rnz 17 38 and and CC cord-258014-lzzi4rnz 17 39 vaginal vaginal JJ cord-258014-lzzi4rnz 17 40 pH ph NN cord-258014-lzzi4rnz 17 41 [ [ -LRB- cord-258014-lzzi4rnz 17 42 12 12 CD cord-258014-lzzi4rnz 17 43 , , , cord-258014-lzzi4rnz 17 44 13 13 CD cord-258014-lzzi4rnz 17 45 ] ] -RRB- cord-258014-lzzi4rnz 17 46 , , , cord-258014-lzzi4rnz 17 47 play play VBP cord-258014-lzzi4rnz 17 48 a a DT cord-258014-lzzi4rnz 17 49 role role NN cord-258014-lzzi4rnz 17 50 in in IN cord-258014-lzzi4rnz 17 51 the the DT cord-258014-lzzi4rnz 17 52 susceptibility susceptibility NN cord-258014-lzzi4rnz 17 53 to to IN cord-258014-lzzi4rnz 17 54 cervical cervical JJ cord-258014-lzzi4rnz 17 55 Human Human NNP cord-258014-lzzi4rnz 17 56 Papillomavirus Papillomavirus NNP cord-258014-lzzi4rnz 17 57 ( ( -LRB- cord-258014-lzzi4rnz 17 58 HPV HPV NNP cord-258014-lzzi4rnz 17 59 ) ) -RRB- cord-258014-lzzi4rnz 17 60 infection infection NN cord-258014-lzzi4rnz 17 61 and and CC cord-258014-lzzi4rnz 17 62 the the DT cord-258014-lzzi4rnz 17 63 development development NN cord-258014-lzzi4rnz 17 64 of of IN cord-258014-lzzi4rnz 17 65 cervical cervical JJ cord-258014-lzzi4rnz 17 66 intraepithelial intraepithelial JJ cord-258014-lzzi4rnz 17 67 neoplasia neoplasia NN cord-258014-lzzi4rnz 17 68 due due IN cord-258014-lzzi4rnz 17 69 to to IN cord-258014-lzzi4rnz 17 70 the the DT cord-258014-lzzi4rnz 17 71 profound profound JJ cord-258014-lzzi4rnz 17 72 shifts shift NNS cord-258014-lzzi4rnz 17 73 in in IN cord-258014-lzzi4rnz 17 74 the the DT cord-258014-lzzi4rnz 17 75 relative relative JJ cord-258014-lzzi4rnz 17 76 abundances abundance NNS cord-258014-lzzi4rnz 17 77 of of IN cord-258014-lzzi4rnz 17 78 protective protective JJ cord-258014-lzzi4rnz 17 79 bacteria bacteria NNS cord-258014-lzzi4rnz 17 80 [ [ -LRB- cord-258014-lzzi4rnz 17 81 10 10 CD cord-258014-lzzi4rnz 17 82 ] ] -RRB- cord-258014-lzzi4rnz 17 83 . . . cord-258014-lzzi4rnz 18 1 Although although IN cord-258014-lzzi4rnz 18 2 cervical cervical JJ cord-258014-lzzi4rnz 18 3 cancer cancer NN cord-258014-lzzi4rnz 18 4 is be VBZ cord-258014-lzzi4rnz 18 5 preventable preventable JJ cord-258014-lzzi4rnz 18 6 mainly mainly RB cord-258014-lzzi4rnz 18 7 by by IN cord-258014-lzzi4rnz 18 8 vaccination vaccination NN cord-258014-lzzi4rnz 18 9 , , , cord-258014-lzzi4rnz 18 10 detection detection NN cord-258014-lzzi4rnz 18 11 , , , cord-258014-lzzi4rnz 18 12 and and CC cord-258014-lzzi4rnz 18 13 treatment treatment NN cord-258014-lzzi4rnz 18 14 of of IN cord-258014-lzzi4rnz 18 15 lesions lesion NNS cord-258014-lzzi4rnz 18 16 , , , cord-258014-lzzi4rnz 18 17 it -PRON- PRP cord-258014-lzzi4rnz 18 18 remains remain VBZ cord-258014-lzzi4rnz 18 19 an an DT cord-258014-lzzi4rnz 18 20 important important JJ cord-258014-lzzi4rnz 18 21 public public JJ cord-258014-lzzi4rnz 18 22 health health NN cord-258014-lzzi4rnz 18 23 problem problem NN cord-258014-lzzi4rnz 18 24 , , , cord-258014-lzzi4rnz 18 25 being be VBG cord-258014-lzzi4rnz 18 26 the the DT cord-258014-lzzi4rnz 18 27 fourth fourth JJ cord-258014-lzzi4rnz 18 28 and and CC cord-258014-lzzi4rnz 18 29 the the DT cord-258014-lzzi4rnz 18 30 second second JJ cord-258014-lzzi4rnz 18 31 most most RBS cord-258014-lzzi4rnz 18 32 common common JJ cord-258014-lzzi4rnz 18 33 cancer cancer NN cord-258014-lzzi4rnz 18 34 in in IN cord-258014-lzzi4rnz 18 35 women woman NNS cord-258014-lzzi4rnz 18 36 globally globally RB cord-258014-lzzi4rnz 18 37 and and CC cord-258014-lzzi4rnz 18 38 in in IN cord-258014-lzzi4rnz 18 39 developing develop VBG cord-258014-lzzi4rnz 18 40 countries country NNS cord-258014-lzzi4rnz 18 41 , , , cord-258014-lzzi4rnz 18 42 respectively respectively RB cord-258014-lzzi4rnz 18 43 ( ( -LRB- cord-258014-lzzi4rnz 18 44 1 1 CD cord-258014-lzzi4rnz 18 45 ) ) -RRB- cord-258014-lzzi4rnz 18 46 . . . cord-258014-lzzi4rnz 19 1 In in IN cord-258014-lzzi4rnz 19 2 Latin Latin NNP cord-258014-lzzi4rnz 19 3 America America NNP cord-258014-lzzi4rnz 19 4 and and CC cord-258014-lzzi4rnz 19 5 the the DT cord-258014-lzzi4rnz 19 6 Caribbean Caribbean NNP cord-258014-lzzi4rnz 19 7 , , , cord-258014-lzzi4rnz 19 8 it -PRON- PRP cord-258014-lzzi4rnz 19 9 remains remain VBZ cord-258014-lzzi4rnz 19 10 the the DT cord-258014-lzzi4rnz 19 11 number number NN cord-258014-lzzi4rnz 19 12 one one CD cord-258014-lzzi4rnz 19 13 cause cause NN cord-258014-lzzi4rnz 19 14 of of IN cord-258014-lzzi4rnz 19 15 mortality mortality NN cord-258014-lzzi4rnz 19 16 due due IN cord-258014-lzzi4rnz 19 17 to to IN cord-258014-lzzi4rnz 19 18 malignant malignant JJ cord-258014-lzzi4rnz 19 19 neoplasms neoplasm NNS cord-258014-lzzi4rnz 19 20 among among IN cord-258014-lzzi4rnz 19 21 20-to 20-to CD cord-258014-lzzi4rnz 19 22 40-year 40-year JJ cord-258014-lzzi4rnz 19 23 - - HYPH cord-258014-lzzi4rnz 19 24 old old JJ cord-258014-lzzi4rnz 19 25 women woman NNS cord-258014-lzzi4rnz 19 26 ( ( -LRB- cord-258014-lzzi4rnz 19 27 2 2 CD cord-258014-lzzi4rnz 19 28 ) ) -RRB- cord-258014-lzzi4rnz 19 29 , , , cord-258014-lzzi4rnz 19 30 three three CD cord-258014-lzzi4rnz 19 31 times time NNS cord-258014-lzzi4rnz 19 32 higher high JJR cord-258014-lzzi4rnz 19 33 in in IN cord-258014-lzzi4rnz 19 34 Latin Latin NNP cord-258014-lzzi4rnz 19 35 America America NNP cord-258014-lzzi4rnz 19 36 than than IN cord-258014-lzzi4rnz 19 37 in in IN cord-258014-lzzi4rnz 19 38 the the DT cord-258014-lzzi4rnz 19 39 United United NNP cord-258014-lzzi4rnz 19 40 States States NNP cord-258014-lzzi4rnz 19 41 ( ( -LRB- cord-258014-lzzi4rnz 19 42 US US NNP cord-258014-lzzi4rnz 19 43 ) ) -RRB- cord-258014-lzzi4rnz 19 44 ( ( -LRB- cord-258014-lzzi4rnz 19 45 3 3 LS cord-258014-lzzi4rnz 19 46 ) ) -RRB- cord-258014-lzzi4rnz 19 47 . . . cord-258014-lzzi4rnz 20 1 According accord VBG cord-258014-lzzi4rnz 20 2 to to IN cord-258014-lzzi4rnz 20 3 guidelines guideline NNS cord-258014-lzzi4rnz 20 4 established establish VBN cord-258014-lzzi4rnz 20 5 by by IN cord-258014-lzzi4rnz 20 6 Healthy Healthy NNP cord-258014-lzzi4rnz 20 7 People People NNPS cord-258014-lzzi4rnz 20 8 [ [ -LRB- cord-258014-lzzi4rnz 20 9 2 2 CD cord-258014-lzzi4rnz 20 10 ] ] -RRB- cord-258014-lzzi4rnz 20 11 , , , cord-258014-lzzi4rnz 20 12 women woman NNS cord-258014-lzzi4rnz 20 13 that that WDT cord-258014-lzzi4rnz 20 14 have have VBP cord-258014-lzzi4rnz 20 15 had have VBN cord-258014-lzzi4rnz 20 16 a a DT cord-258014-lzzi4rnz 20 17 Pap Pap NNP cord-258014-lzzi4rnz 20 18 test test NN cord-258014-lzzi4rnz 20 19 in in IN cord-258014-lzzi4rnz 20 20 three three CD cord-258014-lzzi4rnz 20 21 subsequent subsequent JJ cord-258014-lzzi4rnz 20 22 years year NNS cord-258014-lzzi4rnz 20 23 need need VBP cord-258014-lzzi4rnz 20 24 to to TO cord-258014-lzzi4rnz 20 25 be be VB cord-258014-lzzi4rnz 20 26 at~90 at~90 NNP cord-258014-lzzi4rnz 20 27 % % NN cord-258014-lzzi4rnz 20 28 , , , cord-258014-lzzi4rnz 20 29 however however RB cord-258014-lzzi4rnz 20 30 , , , cord-258014-lzzi4rnz 20 31 in in IN cord-258014-lzzi4rnz 20 32 some some DT cord-258014-lzzi4rnz 20 33 areas area NNS cord-258014-lzzi4rnz 20 34 of of IN cord-258014-lzzi4rnz 20 35 the the DT cord-258014-lzzi4rnz 20 36 world world NN cord-258014-lzzi4rnz 20 37 such such JJ cord-258014-lzzi4rnz 20 38 as as IN cord-258014-lzzi4rnz 20 39 Latin Latin NNP cord-258014-lzzi4rnz 20 40 America America NNP cord-258014-lzzi4rnz 20 41 , , , cord-258014-lzzi4rnz 20 42 this this DT cord-258014-lzzi4rnz 20 43 does do VBZ cord-258014-lzzi4rnz 20 44 not not RB cord-258014-lzzi4rnz 20 45 happen happen VB cord-258014-lzzi4rnz 20 46 , , , cord-258014-lzzi4rnz 20 47 with with IN cord-258014-lzzi4rnz 20 48 women woman NNS cord-258014-lzzi4rnz 20 49 often often RB cord-258014-lzzi4rnz 20 50 lost lose VBD cord-258014-lzzi4rnz 20 51 to to TO cord-258014-lzzi4rnz 20 52 follow follow VB cord-258014-lzzi4rnz 20 53 - - HYPH cord-258014-lzzi4rnz 20 54 up up NN cord-258014-lzzi4rnz 20 55 even even RB cord-258014-lzzi4rnz 20 56 before before IN cord-258014-lzzi4rnz 20 57 treatment treatment NN cord-258014-lzzi4rnz 20 58 occurs occur VBZ cord-258014-lzzi4rnz 20 59 . . . cord-258014-lzzi4rnz 21 1 Guidelines guideline NNS cord-258014-lzzi4rnz 21 2 for for IN cord-258014-lzzi4rnz 21 3 genital genital JJ cord-258014-lzzi4rnz 21 4 care care NN cord-258014-lzzi4rnz 21 5 include include VBP cord-258014-lzzi4rnz 21 6 coming come VBG cord-258014-lzzi4rnz 21 7 to to IN cord-258014-lzzi4rnz 21 8 clinics clinic NNS cord-258014-lzzi4rnz 21 9 for for IN cord-258014-lzzi4rnz 21 10 a a DT cord-258014-lzzi4rnz 21 11 Pap Pap NNP cord-258014-lzzi4rnz 21 12 smear smear NN cord-258014-lzzi4rnz 21 13 for for IN cord-258014-lzzi4rnz 21 14 HPV hpv NN cord-258014-lzzi4rnz 21 15 typing typing NN cord-258014-lzzi4rnz 21 16 and and CC cord-258014-lzzi4rnz 21 17 cervical cervical JJ cord-258014-lzzi4rnz 21 18 cytology cytology NN cord-258014-lzzi4rnz 21 19 , , , cord-258014-lzzi4rnz 21 20 and and CC cord-258014-lzzi4rnz 21 21 for for IN cord-258014-lzzi4rnz 21 22 those those DT cord-258014-lzzi4rnz 21 23 who who WP cord-258014-lzzi4rnz 21 24 meet meet VBP cord-258014-lzzi4rnz 21 25 the the DT cord-258014-lzzi4rnz 21 26 guidelines guideline NNS cord-258014-lzzi4rnz 21 27 , , , cord-258014-lzzi4rnz 21 28 a a DT cord-258014-lzzi4rnz 21 29 return return NN cord-258014-lzzi4rnz 21 30 for for IN cord-258014-lzzi4rnz 21 31 a a DT cord-258014-lzzi4rnz 21 32 second second JJ cord-258014-lzzi4rnz 21 33 visit visit NN cord-258014-lzzi4rnz 21 34 to to TO cord-258014-lzzi4rnz 21 35 receive receive VB cord-258014-lzzi4rnz 21 36 colposcopy colposcopy NN cord-258014-lzzi4rnz 21 37 and and CC cord-258014-lzzi4rnz 21 38 biopsy biopsy NN cord-258014-lzzi4rnz 21 39 . . . cord-258014-lzzi4rnz 22 1 These these DT cord-258014-lzzi4rnz 22 2 are be VBP cord-258014-lzzi4rnz 22 3 invasive invasive JJ cord-258014-lzzi4rnz 22 4 and and CC cord-258014-lzzi4rnz 22 5 expensive expensive JJ cord-258014-lzzi4rnz 22 6 methods method NNS cord-258014-lzzi4rnz 22 7 , , , cord-258014-lzzi4rnz 22 8 and and CC cord-258014-lzzi4rnz 22 9 the the DT cord-258014-lzzi4rnz 22 10 approaches approach NNS cord-258014-lzzi4rnz 22 11 explained explain VBD cord-258014-lzzi4rnz 22 12 in in IN cord-258014-lzzi4rnz 22 13 this this DT cord-258014-lzzi4rnz 22 14 article article NN cord-258014-lzzi4rnz 22 15 are be VBP cord-258014-lzzi4rnz 22 16 innovative innovative JJ cord-258014-lzzi4rnz 22 17 and and CC cord-258014-lzzi4rnz 22 18 have have VBP cord-258014-lzzi4rnz 22 19 the the DT cord-258014-lzzi4rnz 22 20 potential potential NN cord-258014-lzzi4rnz 22 21 to to TO cord-258014-lzzi4rnz 22 22 be be VB cord-258014-lzzi4rnz 22 23 useful useful JJ cord-258014-lzzi4rnz 22 24 in in IN cord-258014-lzzi4rnz 22 25 advancing advance VBG cord-258014-lzzi4rnz 22 26 the the DT cord-258014-lzzi4rnz 22 27 field field NN cord-258014-lzzi4rnz 22 28 . . . cord-258014-lzzi4rnz 23 1 A a DT cord-258014-lzzi4rnz 23 2 persistent persistent JJ cord-258014-lzzi4rnz 23 3 infection infection NN cord-258014-lzzi4rnz 23 4 with with IN cord-258014-lzzi4rnz 23 5 high high JJ cord-258014-lzzi4rnz 23 6 - - HYPH cord-258014-lzzi4rnz 23 7 risk risk NN cord-258014-lzzi4rnz 23 8 human human JJ cord-258014-lzzi4rnz 23 9 papillomavirus papillomavirus NN cord-258014-lzzi4rnz 23 10 ( ( -LRB- cord-258014-lzzi4rnz 23 11 hr hr NN cord-258014-lzzi4rnz 23 12 - - HYPH cord-258014-lzzi4rnz 23 13 HPV HPV NNP cord-258014-lzzi4rnz 23 14 ) ) -RRB- cord-258014-lzzi4rnz 23 15 types type NNS cord-258014-lzzi4rnz 23 16 is be VBZ cord-258014-lzzi4rnz 23 17 a a DT cord-258014-lzzi4rnz 23 18 common common JJ cord-258014-lzzi4rnz 23 19 , , , cord-258014-lzzi4rnz 23 20 but but CC cord-258014-lzzi4rnz 23 21 not not RB cord-258014-lzzi4rnz 23 22 sufficient sufficient JJ cord-258014-lzzi4rnz 23 23 to to TO cord-258014-lzzi4rnz 23 24 cause cause VB cord-258014-lzzi4rnz 23 25 cervical cervical JJ cord-258014-lzzi4rnz 23 26 cancer cancer NN cord-258014-lzzi4rnz 23 27 [ [ -LRB- cord-258014-lzzi4rnz 23 28 14 14 CD cord-258014-lzzi4rnz 23 29 , , , cord-258014-lzzi4rnz 23 30 15 15 CD cord-258014-lzzi4rnz 23 31 ] ] -RRB- cord-258014-lzzi4rnz 23 32 . . . cord-258014-lzzi4rnz 24 1 High high JJ cord-258014-lzzi4rnz 24 2 risk risk NN cord-258014-lzzi4rnz 24 3 ( ( -LRB- cord-258014-lzzi4rnz 24 4 hr hr NN cord-258014-lzzi4rnz 24 5 ) ) -RRB- cord-258014-lzzi4rnz 24 6 HPV hpv NN cord-258014-lzzi4rnz 24 7 infection infection NN cord-258014-lzzi4rnz 24 8 often often RB cord-258014-lzzi4rnz 24 9 requires require VBZ cord-258014-lzzi4rnz 24 10 lifestyle lifestyle NN cord-258014-lzzi4rnz 24 11 factors factor NNS cord-258014-lzzi4rnz 24 12 for for IN cord-258014-lzzi4rnz 24 13 cancer cancer NN cord-258014-lzzi4rnz 24 14 development development NN cord-258014-lzzi4rnz 24 15 , , , cord-258014-lzzi4rnz 24 16 such such JJ cord-258014-lzzi4rnz 24 17 as as IN cord-258014-lzzi4rnz 24 18 smoking smoking NN cord-258014-lzzi4rnz 24 19 or or CC cord-258014-lzzi4rnz 24 20 oral oral JJ cord-258014-lzzi4rnz 24 21 contraceptive contraceptive NN cord-258014-lzzi4rnz 24 22 use use NN cord-258014-lzzi4rnz 24 23 [ [ -LRB- cord-258014-lzzi4rnz 24 24 14 14 CD cord-258014-lzzi4rnz 24 25 ] ] -RRB- cord-258014-lzzi4rnz 24 26 , , , cord-258014-lzzi4rnz 24 27 however however RB cord-258014-lzzi4rnz 24 28 , , , cord-258014-lzzi4rnz 24 29 the the DT cord-258014-lzzi4rnz 24 30 reasons reason NNS cord-258014-lzzi4rnz 24 31 for for IN cord-258014-lzzi4rnz 24 32 hr hr NNP cord-258014-lzzi4rnz 24 33 HPV hpv NN cord-258014-lzzi4rnz 24 34 promotion promotion NN cord-258014-lzzi4rnz 24 35 and and CC cord-258014-lzzi4rnz 24 36 persistence persistence NN cord-258014-lzzi4rnz 24 37 are be VBP cord-258014-lzzi4rnz 24 38 not not RB cord-258014-lzzi4rnz 24 39 completely completely RB cord-258014-lzzi4rnz 24 40 understood understand VBN cord-258014-lzzi4rnz 24 41 . . . cord-258014-lzzi4rnz 25 1 A a DT cord-258014-lzzi4rnz 25 2 gap gap NN cord-258014-lzzi4rnz 25 3 in in IN cord-258014-lzzi4rnz 25 4 our -PRON- PRP$ cord-258014-lzzi4rnz 25 5 understanding understanding NN cord-258014-lzzi4rnz 25 6 of of IN cord-258014-lzzi4rnz 25 7 HPV hpv NN cord-258014-lzzi4rnz 25 8 infection infection NN cord-258014-lzzi4rnz 25 9 and and CC cord-258014-lzzi4rnz 25 10 carcinogenesis carcinogenesis NN cord-258014-lzzi4rnz 25 11 resides reside VBZ cord-258014-lzzi4rnz 25 12 in in IN cord-258014-lzzi4rnz 25 13 the the DT cord-258014-lzzi4rnz 25 14 insufficient insufficient JJ cord-258014-lzzi4rnz 25 15 understanding understanding NN cord-258014-lzzi4rnz 25 16 of of IN cord-258014-lzzi4rnz 25 17 the the DT cord-258014-lzzi4rnz 25 18 true true JJ cord-258014-lzzi4rnz 25 19 microbial microbial JJ cord-258014-lzzi4rnz 25 20 community community NN cord-258014-lzzi4rnz 25 21 structure structure NN cord-258014-lzzi4rnz 25 22 , , , cord-258014-lzzi4rnz 25 23 preventing prevent VBG cord-258014-lzzi4rnz 25 24 our -PRON- PRP$ cord-258014-lzzi4rnz 25 25 ability ability NN cord-258014-lzzi4rnz 25 26 to to TO cord-258014-lzzi4rnz 25 27 control control VB cord-258014-lzzi4rnz 25 28 the the DT cord-258014-lzzi4rnz 25 29 microbiota microbiota NN cord-258014-lzzi4rnz 25 30 for for IN cord-258014-lzzi4rnz 25 31 therapeutic therapeutic JJ cord-258014-lzzi4rnz 25 32 purposes purpose NNS cord-258014-lzzi4rnz 25 33 . . . cord-258014-lzzi4rnz 26 1 As as IN cord-258014-lzzi4rnz 26 2 widely widely RB cord-258014-lzzi4rnz 26 3 known know VBN cord-258014-lzzi4rnz 26 4 , , , cord-258014-lzzi4rnz 26 5 the the DT cord-258014-lzzi4rnz 26 6 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 26 7 microbiome microbiome NN cord-258014-lzzi4rnz 26 8 also also RB cord-258014-lzzi4rnz 26 9 plays play VBZ cord-258014-lzzi4rnz 26 10 a a DT cord-258014-lzzi4rnz 26 11 role role NN cord-258014-lzzi4rnz 26 12 in in IN cord-258014-lzzi4rnz 26 13 disease disease NN cord-258014-lzzi4rnz 26 14 pathogenesis pathogenesis NN cord-258014-lzzi4rnz 26 15 in in IN cord-258014-lzzi4rnz 26 16 combination combination NN cord-258014-lzzi4rnz 26 17 with with IN cord-258014-lzzi4rnz 26 18 HPV HPV NNP cord-258014-lzzi4rnz 26 19 , , , cord-258014-lzzi4rnz 26 20 namely namely RB cord-258014-lzzi4rnz 26 21 genital genital JJ cord-258014-lzzi4rnz 26 22 dysbiosis dysbiosis NN cord-258014-lzzi4rnz 26 23 , , , cord-258014-lzzi4rnz 26 24 a a DT cord-258014-lzzi4rnz 26 25 modifiable modifiable JJ cord-258014-lzzi4rnz 26 26 risk risk NN cord-258014-lzzi4rnz 26 27 factor factor NN cord-258014-lzzi4rnz 26 28 for for IN cord-258014-lzzi4rnz 26 29 cervical cervical JJ cord-258014-lzzi4rnz 26 30 disease disease NN cord-258014-lzzi4rnz 26 31 [ [ -LRB- cord-258014-lzzi4rnz 26 32 3 3 CD cord-258014-lzzi4rnz 26 33 ] ] -RRB- cord-258014-lzzi4rnz 26 34 [ [ -LRB- cord-258014-lzzi4rnz 26 35 4 4 CD cord-258014-lzzi4rnz 26 36 ] ] -RRB- cord-258014-lzzi4rnz 26 37 [ [ -LRB- cord-258014-lzzi4rnz 26 38 5 5 CD cord-258014-lzzi4rnz 26 39 ] ] -RRB- cord-258014-lzzi4rnz 26 40 [ [ -LRB- cord-258014-lzzi4rnz 26 41 6 6 CD cord-258014-lzzi4rnz 26 42 ] ] -RRB- cord-258014-lzzi4rnz 26 43 . . . cord-258014-lzzi4rnz 27 1 A a DT cord-258014-lzzi4rnz 27 2 comparison comparison NN cord-258014-lzzi4rnz 27 3 of of IN cord-258014-lzzi4rnz 27 4 the the DT cord-258014-lzzi4rnz 27 5 vaginal vaginal JJ cord-258014-lzzi4rnz 27 6 microbiome microbiome NN cord-258014-lzzi4rnz 27 7 in in IN cord-258014-lzzi4rnz 27 8 women woman NNS cord-258014-lzzi4rnz 27 9 of of IN cord-258014-lzzi4rnz 27 10 different different JJ cord-258014-lzzi4rnz 27 11 ethnicities ethnicity NNS cord-258014-lzzi4rnz 27 12 within within IN cord-258014-lzzi4rnz 27 13 the the DT cord-258014-lzzi4rnz 27 14 U.S. U.S. NNP cord-258014-lzzi4rnz 27 15 mainland mainland NN cord-258014-lzzi4rnz 27 16 indicates indicate VBZ cord-258014-lzzi4rnz 27 17 different different JJ cord-258014-lzzi4rnz 27 18 relative relative JJ cord-258014-lzzi4rnz 27 19 abundances abundance NNS cord-258014-lzzi4rnz 27 20 of of IN cord-258014-lzzi4rnz 27 21 the the DT cord-258014-lzzi4rnz 27 22 four four CD cord-258014-lzzi4rnz 27 23 main main JJ cord-258014-lzzi4rnz 27 24 groups group NNS cord-258014-lzzi4rnz 27 25 of of IN cord-258014-lzzi4rnz 27 26 Lactobacillus lactobacillus NN cord-258014-lzzi4rnz 27 27 and and CC cord-258014-lzzi4rnz 27 28 anaerobic anaerobic JJ cord-258014-lzzi4rnz 27 29 bacteria bacteria NNS cord-258014-lzzi4rnz 27 30 [ [ -LRB- cord-258014-lzzi4rnz 27 31 4 4 CD cord-258014-lzzi4rnz 27 32 ] ] -RRB- cord-258014-lzzi4rnz 27 33 . . . cord-258014-lzzi4rnz 28 1 Different different JJ cord-258014-lzzi4rnz 28 2 studies study NNS cord-258014-lzzi4rnz 28 3 have have VBP cord-258014-lzzi4rnz 28 4 described describe VBN cord-258014-lzzi4rnz 28 5 that that IN cord-258014-lzzi4rnz 28 6 infections infection NNS cord-258014-lzzi4rnz 28 7 with with IN cord-258014-lzzi4rnz 28 8 HPV HPV NNP cord-258014-lzzi4rnz 28 9 are be VBP cord-258014-lzzi4rnz 28 10 associated associate VBN cord-258014-lzzi4rnz 28 11 with with IN cord-258014-lzzi4rnz 28 12 an an DT cord-258014-lzzi4rnz 28 13 increase increase NN cord-258014-lzzi4rnz 28 14 in in IN cord-258014-lzzi4rnz 28 15 Atopobium Atopobium NNP cord-258014-lzzi4rnz 28 16 vaginae vaginae NNP cord-258014-lzzi4rnz 28 17 and and CC cord-258014-lzzi4rnz 28 18 G. G. NNP cord-258014-lzzi4rnz 28 19 vaginallis vaginallis NNP cord-258014-lzzi4rnz 28 20 [ [ -LRB- cord-258014-lzzi4rnz 28 21 5 5 CD cord-258014-lzzi4rnz 28 22 , , , cord-258014-lzzi4rnz 28 23 11 11 CD cord-258014-lzzi4rnz 28 24 ] ] -RRB- cord-258014-lzzi4rnz 28 25 . . . cord-258014-lzzi4rnz 29 1 Other other JJ cord-258014-lzzi4rnz 29 2 Latino latino JJ cord-258014-lzzi4rnz 29 3 populations population NNS cord-258014-lzzi4rnz 29 4 , , , cord-258014-lzzi4rnz 29 5 like like IN cord-258014-lzzi4rnz 29 6 Mexicans Mexicans NNPS cord-258014-lzzi4rnz 29 7 , , , cord-258014-lzzi4rnz 29 8 have have VBP cord-258014-lzzi4rnz 29 9 shown show VBN cord-258014-lzzi4rnz 29 10 Sneathia Sneathia NNP cord-258014-lzzi4rnz 29 11 spp spp NNS cord-258014-lzzi4rnz 29 12 . . . cord-258014-lzzi4rnz 30 1 as as IN cord-258014-lzzi4rnz 30 2 a a DT cord-258014-lzzi4rnz 30 3 cervical cervical JJ cord-258014-lzzi4rnz 30 4 biomarker biomarker NN cord-258014-lzzi4rnz 30 5 for for IN cord-258014-lzzi4rnz 30 6 neoplasia neoplasia NN cord-258014-lzzi4rnz 30 7 [ [ -LRB- cord-258014-lzzi4rnz 30 8 7 7 CD cord-258014-lzzi4rnz 30 9 ] ] -RRB- cord-258014-lzzi4rnz 30 10 . . . cord-258014-lzzi4rnz 31 1 Not not RB cord-258014-lzzi4rnz 31 2 only only RB cord-258014-lzzi4rnz 31 3 is be VBZ cord-258014-lzzi4rnz 31 4 bacterial bacterial JJ cord-258014-lzzi4rnz 31 5 biota biota NN cord-258014-lzzi4rnz 31 6 associated associate VBN cord-258014-lzzi4rnz 31 7 with with IN cord-258014-lzzi4rnz 31 8 HPV HPV NNP cord-258014-lzzi4rnz 31 9 , , , cord-258014-lzzi4rnz 31 10 but but CC cord-258014-lzzi4rnz 31 11 studies study NNS cord-258014-lzzi4rnz 31 12 have have VBP cord-258014-lzzi4rnz 31 13 also also RB cord-258014-lzzi4rnz 31 14 found find VBN cord-258014-lzzi4rnz 31 15 an an DT cord-258014-lzzi4rnz 31 16 important important JJ cord-258014-lzzi4rnz 31 17 role role NN cord-258014-lzzi4rnz 31 18 of of IN cord-258014-lzzi4rnz 31 19 fungal fungal JJ cord-258014-lzzi4rnz 31 20 signatures signature NNS cord-258014-lzzi4rnz 31 21 such such JJ cord-258014-lzzi4rnz 31 22 as as IN cord-258014-lzzi4rnz 31 23 that that DT cord-258014-lzzi4rnz 31 24 of of IN cord-258014-lzzi4rnz 31 25 Malassezia Malassezia NNP cord-258014-lzzi4rnz 31 26 for for IN cord-258014-lzzi4rnz 31 27 hr hr NNP cord-258014-lzzi4rnz 31 28 HPV HPV NNP cord-258014-lzzi4rnz 31 29 infections infection NNS cord-258014-lzzi4rnz 31 30 and and CC cord-258014-lzzi4rnz 31 31 an an DT cord-258014-lzzi4rnz 31 32 increase increase NN cord-258014-lzzi4rnz 31 33 in in IN cord-258014-lzzi4rnz 31 34 Sporidiobolaceae Sporidiobolaceae NNP cord-258014-lzzi4rnz 31 35 and and CC cord-258014-lzzi4rnz 31 36 Sacharomyces Sacharomyces NNPS cord-258014-lzzi4rnz 31 37 in in IN cord-258014-lzzi4rnz 31 38 atypical atypical JJ cord-258014-lzzi4rnz 31 39 squamous squamous JJ cord-258014-lzzi4rnz 31 40 cells cell NNS cord-258014-lzzi4rnz 31 41 of of IN cord-258014-lzzi4rnz 31 42 undetermined undetermined JJ cord-258014-lzzi4rnz 31 43 significance significance NN cord-258014-lzzi4rnz 31 44 ( ( -LRB- cord-258014-lzzi4rnz 31 45 ASCUS ASCUS NNP cord-258014-lzzi4rnz 31 46 ) ) -RRB- cord-258014-lzzi4rnz 31 47 lesions lesion NNS cord-258014-lzzi4rnz 31 48 [ [ -LRB- cord-258014-lzzi4rnz 31 49 5 5 CD cord-258014-lzzi4rnz 31 50 , , , cord-258014-lzzi4rnz 31 51 16 16 CD cord-258014-lzzi4rnz 31 52 ] ] -RRB- cord-258014-lzzi4rnz 31 53 . . . cord-258014-lzzi4rnz 32 1 Thus thus RB cord-258014-lzzi4rnz 32 2 , , , cord-258014-lzzi4rnz 32 3 non non JJ cord-258014-lzzi4rnz 32 4 - - JJ cord-258014-lzzi4rnz 32 5 Lactobacillus lactobacillus JJ cord-258014-lzzi4rnz 32 6 dominant dominant JJ cord-258014-lzzi4rnz 32 7 communities community NNS cord-258014-lzzi4rnz 32 8 , , , cord-258014-lzzi4rnz 32 9 with with IN cord-258014-lzzi4rnz 32 10 a a DT cord-258014-lzzi4rnz 32 11 concomitant concomitant JJ cord-258014-lzzi4rnz 32 12 increase increase NN cord-258014-lzzi4rnz 32 13 in in IN cord-258014-lzzi4rnz 32 14 pH ph NN cord-258014-lzzi4rnz 32 15 and and CC cord-258014-lzzi4rnz 32 16 related relate VBN cord-258014-lzzi4rnz 32 17 to to IN cord-258014-lzzi4rnz 32 18 high high JJ cord-258014-lzzi4rnz 32 19 - - HYPH cord-258014-lzzi4rnz 32 20 grade grade NN cord-258014-lzzi4rnz 32 21 dysplasia dysplasia NN cord-258014-lzzi4rnz 32 22 , , , cord-258014-lzzi4rnz 32 23 perturb perturb VB cord-258014-lzzi4rnz 32 24 amino amino NN cord-258014-lzzi4rnz 32 25 acid acid NN cord-258014-lzzi4rnz 32 26 and and CC cord-258014-lzzi4rnz 32 27 nucleotide nucleotide JJ cord-258014-lzzi4rnz 32 28 metabolisms metabolism NNS cord-258014-lzzi4rnz 32 29 and and CC cord-258014-lzzi4rnz 32 30 drive drive VB cord-258014-lzzi4rnz 32 31 inflammation inflammation NN cord-258014-lzzi4rnz 32 32 and and CC cord-258014-lzzi4rnz 32 33 HPV hpv NN cord-258014-lzzi4rnz 32 34 infections infection NNS cord-258014-lzzi4rnz 32 35 . . . cord-258014-lzzi4rnz 33 1 The the DT cord-258014-lzzi4rnz 33 2 combination combination NN cord-258014-lzzi4rnz 33 3 of of IN cord-258014-lzzi4rnz 33 4 the the DT cord-258014-lzzi4rnz 33 5 microbiome microbiome NN cord-258014-lzzi4rnz 33 6 with with IN cord-258014-lzzi4rnz 33 7 metabolome metabolome NNP cord-258014-lzzi4rnz 33 8 can can MD cord-258014-lzzi4rnz 33 9 become become VB cord-258014-lzzi4rnz 33 10 even even RB cord-258014-lzzi4rnz 33 11 more more RBR cord-258014-lzzi4rnz 33 12 informative informative JJ cord-258014-lzzi4rnz 33 13 than than IN cord-258014-lzzi4rnz 33 14 classical classical JJ cord-258014-lzzi4rnz 33 15 HPV HPV NNP cord-258014-lzzi4rnz 33 16 typing typing NN cord-258014-lzzi4rnz 33 17 . . . cord-258014-lzzi4rnz 34 1 Recently recently RB cord-258014-lzzi4rnz 34 2 , , , cord-258014-lzzi4rnz 34 3 microbial microbial JJ cord-258014-lzzi4rnz 34 4 inventories inventory NNS cord-258014-lzzi4rnz 34 5 via via IN cord-258014-lzzi4rnz 34 6 sequencing sequencing NN cord-258014-lzzi4rnz 34 7 of of IN cord-258014-lzzi4rnz 34 8 16S 16S NNP cord-258014-lzzi4rnz 34 9 rRNA rRNA NNP cord-258014-lzzi4rnz 34 10 genes gene NNS cord-258014-lzzi4rnz 34 11 , , , cord-258014-lzzi4rnz 34 12 have have VBP cord-258014-lzzi4rnz 34 13 been be VBN cord-258014-lzzi4rnz 34 14 complemented complement VBN cord-258014-lzzi4rnz 34 15 with with IN cord-258014-lzzi4rnz 34 16 metabolomics metabolomic NNS cord-258014-lzzi4rnz 34 17 , , , cord-258014-lzzi4rnz 34 18 adding add VBG cord-258014-lzzi4rnz 34 19 a a DT cord-258014-lzzi4rnz 34 20 layer layer NN cord-258014-lzzi4rnz 34 21 of of IN cord-258014-lzzi4rnz 34 22 information information NN cord-258014-lzzi4rnz 34 23 regarding regard VBG cord-258014-lzzi4rnz 34 24 the the DT cord-258014-lzzi4rnz 34 25 metabolic metabolic JJ cord-258014-lzzi4rnz 34 26 activities activity NNS cord-258014-lzzi4rnz 34 27 of of IN cord-258014-lzzi4rnz 34 28 the the DT cord-258014-lzzi4rnz 34 29 microbiome microbiome NN cord-258014-lzzi4rnz 34 30 that that WDT cord-258014-lzzi4rnz 34 31 helps help VBZ cord-258014-lzzi4rnz 34 32 elucidate elucidate VB cord-258014-lzzi4rnz 34 33 the the DT cord-258014-lzzi4rnz 34 34 etiology etiology NN cord-258014-lzzi4rnz 34 35 of of IN cord-258014-lzzi4rnz 34 36 the the DT cord-258014-lzzi4rnz 34 37 microbiome microbiome NN cord-258014-lzzi4rnz 34 38 - - HYPH cord-258014-lzzi4rnz 34 39 mediated mediate VBN cord-258014-lzzi4rnz 34 40 disease disease NN cord-258014-lzzi4rnz 34 41 [ [ -LRB- cord-258014-lzzi4rnz 34 42 17 17 CD cord-258014-lzzi4rnz 34 43 , , , cord-258014-lzzi4rnz 34 44 18 18 CD cord-258014-lzzi4rnz 34 45 ] ] -RRB- cord-258014-lzzi4rnz 34 46 . . . cord-258014-lzzi4rnz 35 1 Metabolites metabolite NNS cord-258014-lzzi4rnz 35 2 produced produce VBN cord-258014-lzzi4rnz 35 3 by by IN cord-258014-lzzi4rnz 35 4 commensal commensal JJ cord-258014-lzzi4rnz 35 5 microorganisms microorganism NNS cord-258014-lzzi4rnz 35 6 may may MD cord-258014-lzzi4rnz 35 7 affect affect VB cord-258014-lzzi4rnz 35 8 host host NN cord-258014-lzzi4rnz 35 9 metabolic metabolic JJ cord-258014-lzzi4rnz 35 10 processes process NNS cord-258014-lzzi4rnz 35 11 , , , cord-258014-lzzi4rnz 35 12 with with IN cord-258014-lzzi4rnz 35 13 both both CC cord-258014-lzzi4rnz 35 14 pathogenic pathogenic JJ cord-258014-lzzi4rnz 35 15 and and CC cord-258014-lzzi4rnz 35 16 protective protective JJ cord-258014-lzzi4rnz 35 17 consequences consequence NNS cord-258014-lzzi4rnz 35 18 . . . cord-258014-lzzi4rnz 36 1 Multi multi JJ cord-258014-lzzi4rnz 36 2 - - JJ cord-258014-lzzi4rnz 36 3 kingdom kingdom JJ cord-258014-lzzi4rnz 36 4 colonization colonization NN cord-258014-lzzi4rnz 36 5 ( ( -LRB- cord-258014-lzzi4rnz 36 6 bacteria bacteria NNS cord-258014-lzzi4rnz 36 7 , , , cord-258014-lzzi4rnz 36 8 Archaea Archaea NNP cord-258014-lzzi4rnz 36 9 , , , cord-258014-lzzi4rnz 36 10 fungi fungus NNS cord-258014-lzzi4rnz 36 11 , , , cord-258014-lzzi4rnz 36 12 and and CC cord-258014-lzzi4rnz 36 13 viruses virus NNS cord-258014-lzzi4rnz 36 14 ) ) -RRB- cord-258014-lzzi4rnz 36 15 associated associate VBN cord-258014-lzzi4rnz 36 16 with with IN cord-258014-lzzi4rnz 36 17 HPV HPV NNP cord-258014-lzzi4rnz 36 18 infections infection NNS cord-258014-lzzi4rnz 36 19 in in IN cord-258014-lzzi4rnz 36 20 the the DT cord-258014-lzzi4rnz 36 21 vagina vagina NN cord-258014-lzzi4rnz 36 22 and and CC cord-258014-lzzi4rnz 36 23 cervix cervix NN cord-258014-lzzi4rnz 36 24 can can MD cord-258014-lzzi4rnz 36 25 produce produce VB cord-258014-lzzi4rnz 36 26 metabolites metabolite NNS cord-258014-lzzi4rnz 36 27 which which WDT cord-258014-lzzi4rnz 36 28 translocate translocate VBP cord-258014-lzzi4rnz 36 29 through through IN cord-258014-lzzi4rnz 36 30 the the DT cord-258014-lzzi4rnz 36 31 host host NN cord-258014-lzzi4rnz 36 32 's 's POS cord-258014-lzzi4rnz 36 33 barriers barrier NNS cord-258014-lzzi4rnz 36 34 , , , cord-258014-lzzi4rnz 36 35 that that WDT cord-258014-lzzi4rnz 36 36 could could MD cord-258014-lzzi4rnz 36 37 influence influence VB cord-258014-lzzi4rnz 36 38 systemic systemic JJ cord-258014-lzzi4rnz 36 39 health health NN cord-258014-lzzi4rnz 36 40 effects effect NNS cord-258014-lzzi4rnz 36 41 in in IN cord-258014-lzzi4rnz 36 42 women woman NNS cord-258014-lzzi4rnz 36 43 at at IN cord-258014-lzzi4rnz 36 44 risk risk NN cord-258014-lzzi4rnz 36 45 of of IN cord-258014-lzzi4rnz 36 46 developing develop VBG cord-258014-lzzi4rnz 36 47 cervical cervical JJ cord-258014-lzzi4rnz 36 48 cancer cancer NN cord-258014-lzzi4rnz 36 49 . . . cord-258014-lzzi4rnz 37 1 Indeed indeed RB cord-258014-lzzi4rnz 37 2 , , , cord-258014-lzzi4rnz 37 3 recent recent JJ cord-258014-lzzi4rnz 37 4 findings finding NNS cord-258014-lzzi4rnz 37 5 suggest suggest VBP cord-258014-lzzi4rnz 37 6 that that IN cord-258014-lzzi4rnz 37 7 cervical cervical JJ cord-258014-lzzi4rnz 37 8 microbial microbial JJ cord-258014-lzzi4rnz 37 9 metabolic metabolic JJ cord-258014-lzzi4rnz 37 10 changes change NNS cord-258014-lzzi4rnz 37 11 may may MD cord-258014-lzzi4rnz 37 12 influence influence VB cord-258014-lzzi4rnz 37 13 systemic systemic JJ cord-258014-lzzi4rnz 37 14 health health NN cord-258014-lzzi4rnz 37 15 effects effect NNS cord-258014-lzzi4rnz 37 16 in in IN cord-258014-lzzi4rnz 37 17 women woman NNS cord-258014-lzzi4rnz 37 18 at at IN cord-258014-lzzi4rnz 37 19 risk risk NN cord-258014-lzzi4rnz 37 20 of of IN cord-258014-lzzi4rnz 37 21 developing develop VBG cord-258014-lzzi4rnz 37 22 cervical cervical JJ cord-258014-lzzi4rnz 37 23 cancer cancer NN cord-258014-lzzi4rnz 37 24 [ [ -LRB- cord-258014-lzzi4rnz 37 25 18 18 CD cord-258014-lzzi4rnz 37 26 ] ] -RRB- cord-258014-lzzi4rnz 37 27 . . . cord-258014-lzzi4rnz 38 1 Recent recent JJ cord-258014-lzzi4rnz 38 2 data datum NNS cord-258014-lzzi4rnz 38 3 has have VBZ cord-258014-lzzi4rnz 38 4 been be VBN cord-258014-lzzi4rnz 38 5 emerging emerge VBG cord-258014-lzzi4rnz 38 6 on on IN cord-258014-lzzi4rnz 38 7 the the DT cord-258014-lzzi4rnz 38 8 usefulness usefulness NN cord-258014-lzzi4rnz 38 9 of of IN cord-258014-lzzi4rnz 38 10 coupling couple VBG cord-258014-lzzi4rnz 38 11 microbiota microbiota NN cord-258014-lzzi4rnz 38 12 inventories inventory NNS cord-258014-lzzi4rnz 38 13 with with IN cord-258014-lzzi4rnz 38 14 systemic systemic JJ cord-258014-lzzi4rnz 38 15 metabolomics metabolomic NNS cord-258014-lzzi4rnz 38 16 in in IN cord-258014-lzzi4rnz 38 17 biofluids biofluid NNS cord-258014-lzzi4rnz 38 18 such such JJ cord-258014-lzzi4rnz 38 19 as as IN cord-258014-lzzi4rnz 38 20 urine urine NN cord-258014-lzzi4rnz 38 21 ( ( -LRB- cord-258014-lzzi4rnz 38 22 that that DT cord-258014-lzzi4rnz 38 23 can can MD cord-258014-lzzi4rnz 38 24 be be VB cord-258014-lzzi4rnz 38 25 self self NN cord-258014-lzzi4rnz 38 26 - - HYPH cord-258014-lzzi4rnz 38 27 collected collect VBN cord-258014-lzzi4rnz 38 28 ) ) -RRB- cord-258014-lzzi4rnz 38 29 , , , cord-258014-lzzi4rnz 38 30 thus thus RB cord-258014-lzzi4rnz 38 31 easy easy JJ cord-258014-lzzi4rnz 38 32 to to TO cord-258014-lzzi4rnz 38 33 obtain obtain VB cord-258014-lzzi4rnz 38 34 and and CC cord-258014-lzzi4rnz 38 35 minimally minimally RB cord-258014-lzzi4rnz 38 36 invasive invasive JJ cord-258014-lzzi4rnz 38 37 . . . cord-258014-lzzi4rnz 39 1 Indeed indeed RB cord-258014-lzzi4rnz 39 2 , , , cord-258014-lzzi4rnz 39 3 biomarkers biomarker NNS cord-258014-lzzi4rnz 39 4 for for IN cord-258014-lzzi4rnz 39 5 cervical cervical JJ cord-258014-lzzi4rnz 39 6 cancer cancer NN cord-258014-lzzi4rnz 39 7 may may MD cord-258014-lzzi4rnz 39 8 be be VB cord-258014-lzzi4rnz 39 9 exfoliated exfoliate VBN cord-258014-lzzi4rnz 39 10 as as IN cord-258014-lzzi4rnz 39 11 debris debris NN cord-258014-lzzi4rnz 39 12 in in IN cord-258014-lzzi4rnz 39 13 urine urine NN cord-258014-lzzi4rnz 39 14 , , , cord-258014-lzzi4rnz 39 15 a a DT cord-258014-lzzi4rnz 39 16 kind kind NN cord-258014-lzzi4rnz 39 17 of of IN cord-258014-lzzi4rnz 39 18 liquid liquid JJ cord-258014-lzzi4rnz 39 19 biopsy biopsy NN cord-258014-lzzi4rnz 39 20 - - : cord-258014-lzzi4rnz 39 21 which which WDT cord-258014-lzzi4rnz 39 22 could could MD cord-258014-lzzi4rnz 39 23 facilitate facilitate VB cord-258014-lzzi4rnz 39 24 diagnostics diagnostic NNS cord-258014-lzzi4rnz 39 25 [ [ -LRB- cord-258014-lzzi4rnz 39 26 1 1 CD cord-258014-lzzi4rnz 39 27 ] ] -RRB- cord-258014-lzzi4rnz 39 28 . . . cord-258014-lzzi4rnz 40 1 It -PRON- PRP cord-258014-lzzi4rnz 40 2 is be VBZ cord-258014-lzzi4rnz 40 3 , , , cord-258014-lzzi4rnz 40 4 therefore therefore RB cord-258014-lzzi4rnz 40 5 , , , cord-258014-lzzi4rnz 40 6 important important JJ cord-258014-lzzi4rnz 40 7 to to TO cord-258014-lzzi4rnz 40 8 develop develop VB cord-258014-lzzi4rnz 40 9 protocols protocol NNS cord-258014-lzzi4rnz 40 10 to to TO cord-258014-lzzi4rnz 40 11 help help VB cord-258014-lzzi4rnz 40 12 integrate integrate VB cord-258014-lzzi4rnz 40 13 information information NN cord-258014-lzzi4rnz 40 14 between between IN cord-258014-lzzi4rnz 40 15 the the DT cord-258014-lzzi4rnz 40 16 microbiome microbiome NN cord-258014-lzzi4rnz 40 17 , , , cord-258014-lzzi4rnz 40 18 viral viral JJ cord-258014-lzzi4rnz 40 19 pathogenesis pathogenesis NN cord-258014-lzzi4rnz 40 20 , , , cord-258014-lzzi4rnz 40 21 and and CC cord-258014-lzzi4rnz 40 22 immunity immunity NN cord-258014-lzzi4rnz 40 23 affecting affect VBG cord-258014-lzzi4rnz 40 24 cancer cancer NN cord-258014-lzzi4rnz 40 25 progression progression NN cord-258014-lzzi4rnz 40 26 . . . cord-258014-lzzi4rnz 41 1 Additional additional JJ cord-258014-lzzi4rnz 41 2 to to IN cord-258014-lzzi4rnz 41 3 microbiota microbiota NN cord-258014-lzzi4rnz 41 4 analyses analysis NNS cord-258014-lzzi4rnz 41 5 , , , cord-258014-lzzi4rnz 41 6 one one PRP cord-258014-lzzi4rnz 41 7 could could MD cord-258014-lzzi4rnz 41 8 develop develop VB cord-258014-lzzi4rnz 41 9 shotgun shotgun NN cord-258014-lzzi4rnz 41 10 metagenomic metagenomic JJ cord-258014-lzzi4rnz 41 11 methods method NNS cord-258014-lzzi4rnz 41 12 to to TO cord-258014-lzzi4rnz 41 13 understand understand VB cord-258014-lzzi4rnz 41 14 the the DT cord-258014-lzzi4rnz 41 15 full full JJ cord-258014-lzzi4rnz 41 16 diversity diversity NN cord-258014-lzzi4rnz 41 17 more more RBR cord-258014-lzzi4rnz 41 18 accurately accurately RB cord-258014-lzzi4rnz 41 19 , , , cord-258014-lzzi4rnz 41 20 including include VBG cord-258014-lzzi4rnz 41 21 Archaea Archaea NNP cord-258014-lzzi4rnz 41 22 and and CC cord-258014-lzzi4rnz 41 23 viral viral JJ cord-258014-lzzi4rnz 41 24 composition composition NN cord-258014-lzzi4rnz 41 25 affecting affect VBG cord-258014-lzzi4rnz 41 26 the the DT cord-258014-lzzi4rnz 41 27 dysbiotic dysbiotic JJ cord-258014-lzzi4rnz 41 28 or or CC cord-258014-lzzi4rnz 41 29 disease disease NN cord-258014-lzzi4rnz 41 30 status status NN cord-258014-lzzi4rnz 41 31 . . . cord-258014-lzzi4rnz 42 1 Indeed indeed RB cord-258014-lzzi4rnz 42 2 , , , cord-258014-lzzi4rnz 42 3 shotgun shotgun NN cord-258014-lzzi4rnz 42 4 approaches approach NNS cord-258014-lzzi4rnz 42 5 are be VBP cord-258014-lzzi4rnz 42 6 useful useful JJ cord-258014-lzzi4rnz 42 7 methods method NNS cord-258014-lzzi4rnz 42 8 that that WDT cord-258014-lzzi4rnz 42 9 can can MD cord-258014-lzzi4rnz 42 10 non non VB cord-258014-lzzi4rnz 42 11 - - JJ cord-258014-lzzi4rnz 42 12 specifically specifically RB cord-258014-lzzi4rnz 42 13 detect detect VB cord-258014-lzzi4rnz 42 14 HPVs hpvs CD cord-258014-lzzi4rnz 42 15 and and CC cord-258014-lzzi4rnz 42 16 other other JJ cord-258014-lzzi4rnz 42 17 highly highly RB cord-258014-lzzi4rnz 42 18 divergent divergent JJ cord-258014-lzzi4rnz 42 19 viruses virus NNS cord-258014-lzzi4rnz 42 20 ( ( -LRB- cord-258014-lzzi4rnz 42 21 specifically specifically RB cord-258014-lzzi4rnz 42 22 DNA dna NN cord-258014-lzzi4rnz 42 23 viruses virus NNS cord-258014-lzzi4rnz 42 24 ) ) -RRB- cord-258014-lzzi4rnz 42 25 , , , cord-258014-lzzi4rnz 42 26 using use VBG cord-258014-lzzi4rnz 42 27 rolling roll VBG cord-258014-lzzi4rnz 42 28 circle circle NN cord-258014-lzzi4rnz 42 29 amplification amplification NN cord-258014-lzzi4rnz 42 30 ( ( -LRB- cord-258014-lzzi4rnz 42 31 RCA RCA NNP cord-258014-lzzi4rnz 42 32 ) ) -RRB- cord-258014-lzzi4rnz 42 33 [ [ -LRB- cord-258014-lzzi4rnz 42 34 19 19 CD cord-258014-lzzi4rnz 42 35 ] ] -RRB- cord-258014-lzzi4rnz 42 36 , , , cord-258014-lzzi4rnz 42 37 or or CC cord-258014-lzzi4rnz 42 38 even even RB cord-258014-lzzi4rnz 42 39 using use VBG cord-258014-lzzi4rnz 42 40 meta meta JJ cord-258014-lzzi4rnz 42 41 - - HYPH cord-258014-lzzi4rnz 42 42 total total JJ cord-258014-lzzi4rnz 42 43 RNA RNA NNP cord-258014-lzzi4rnz 42 44 sequencing sequencing NN cord-258014-lzzi4rnz 42 45 ( ( -LRB- cord-258014-lzzi4rnz 42 46 MeTRS MeTRS NNP cord-258014-lzzi4rnz 42 47 ) ) -RRB- cord-258014-lzzi4rnz 42 48 methods method NNS cord-258014-lzzi4rnz 42 49 based base VBN cord-258014-lzzi4rnz 42 50 on on IN cord-258014-lzzi4rnz 42 51 shotgun shotgun NN cord-258014-lzzi4rnz 42 52 sequencing sequencing NN cord-258014-lzzi4rnz 42 53 of of IN cord-258014-lzzi4rnz 42 54 total total JJ cord-258014-lzzi4rnz 42 55 RNA RNA NNP cord-258014-lzzi4rnz 42 56 , , , cord-258014-lzzi4rnz 42 57 which which WDT cord-258014-lzzi4rnz 42 58 could could MD cord-258014-lzzi4rnz 42 59 reveal reveal VB cord-258014-lzzi4rnz 42 60 the the DT cord-258014-lzzi4rnz 42 61 extent extent NN cord-258014-lzzi4rnz 42 62 of of IN cord-258014-lzzi4rnz 42 63 RNA RNA NNP cord-258014-lzzi4rnz 42 64 viruses virus NNS cord-258014-lzzi4rnz 42 65 [ [ -LRB- cord-258014-lzzi4rnz 42 66 20 20 CD cord-258014-lzzi4rnz 42 67 ] ] -RRB- cord-258014-lzzi4rnz 42 68 ( ( -LRB- cord-258014-lzzi4rnz 42 69 even even RB cord-258014-lzzi4rnz 42 70 the the DT cord-258014-lzzi4rnz 42 71 novel novel JJ cord-258014-lzzi4rnz 42 72 coronavirus coronavirus NN cord-258014-lzzi4rnz 42 73 - - HYPH cord-258014-lzzi4rnz 42 74 SARS SARS NNP cord-258014-lzzi4rnz 42 75 - - HYPH cord-258014-lzzi4rnz 42 76 CoV-2 CoV-2 NNP cord-258014-lzzi4rnz 42 77 ) ) -RRB- cord-258014-lzzi4rnz 42 78 , , , cord-258014-lzzi4rnz 42 79 which which WDT cord-258014-lzzi4rnz 42 80 could could MD cord-258014-lzzi4rnz 42 81 lead lead VB cord-258014-lzzi4rnz 42 82 to to IN cord-258014-lzzi4rnz 42 83 new new JJ cord-258014-lzzi4rnz 42 84 methodologies methodology NNS cord-258014-lzzi4rnz 42 85 to to TO cord-258014-lzzi4rnz 42 86 reveal reveal VB cord-258014-lzzi4rnz 42 87 the the DT cord-258014-lzzi4rnz 42 88 impact impact NN cord-258014-lzzi4rnz 42 89 of of IN cord-258014-lzzi4rnz 42 90 the the DT cord-258014-lzzi4rnz 42 91 new new JJ cord-258014-lzzi4rnz 42 92 virus virus NN cord-258014-lzzi4rnz 42 93 on on IN cord-258014-lzzi4rnz 42 94 the the DT cord-258014-lzzi4rnz 42 95 microbiome microbiome NN cord-258014-lzzi4rnz 42 96 and and CC cord-258014-lzzi4rnz 42 97 the the DT cord-258014-lzzi4rnz 42 98 immune immune JJ cord-258014-lzzi4rnz 42 99 response response NN cord-258014-lzzi4rnz 42 100 responsible responsible JJ cord-258014-lzzi4rnz 42 101 for for IN cord-258014-lzzi4rnz 42 102 carcinogenesis carcinogenesis NN cord-258014-lzzi4rnz 42 103 . . . cord-258014-lzzi4rnz 43 1 It -PRON- PRP cord-258014-lzzi4rnz 43 2 is be VBZ cord-258014-lzzi4rnz 43 3 crucial crucial JJ cord-258014-lzzi4rnz 43 4 to to TO cord-258014-lzzi4rnz 43 5 develop develop VB cord-258014-lzzi4rnz 43 6 more more JJR cord-258014-lzzi4rnz 43 7 microbiome microbiome NN cord-258014-lzzi4rnz 43 8 / / SYM cord-258014-lzzi4rnz 43 9 metabolome metabolome NN cord-258014-lzzi4rnz 43 10 studies study NNS cord-258014-lzzi4rnz 43 11 to to TO cord-258014-lzzi4rnz 43 12 understand understand VB cord-258014-lzzi4rnz 43 13 cancer cancer NN cord-258014-lzzi4rnz 43 14 progression progression NN cord-258014-lzzi4rnz 43 15 . . . cord-258014-lzzi4rnz 44 1 The the DT cord-258014-lzzi4rnz 44 2 early early JJ cord-258014-lzzi4rnz 44 3 detection detection NN cord-258014-lzzi4rnz 44 4 and and CC cord-258014-lzzi4rnz 44 5 treatment treatment NN cord-258014-lzzi4rnz 44 6 of of IN cord-258014-lzzi4rnz 44 7 genital genital JJ cord-258014-lzzi4rnz 44 8 dysbiosis dysbiosis NN cord-258014-lzzi4rnz 44 9 may may MD cord-258014-lzzi4rnz 44 10 facilitate facilitate VB cord-258014-lzzi4rnz 44 11 better well JJR cord-258014-lzzi4rnz 44 12 management management NN cord-258014-lzzi4rnz 44 13 of of IN cord-258014-lzzi4rnz 44 14 the the DT cord-258014-lzzi4rnz 44 15 disease disease NN cord-258014-lzzi4rnz 44 16 [ [ -LRB- cord-258014-lzzi4rnz 44 17 12 12 CD cord-258014-lzzi4rnz 44 18 ] ] -RRB- cord-258014-lzzi4rnz 44 19 . . . cord-258014-lzzi4rnz 45 1 As as IN cord-258014-lzzi4rnz 45 2 such such JJ cord-258014-lzzi4rnz 45 3 , , , cord-258014-lzzi4rnz 45 4 new new JJ cord-258014-lzzi4rnz 45 5 quick quick JJ cord-258014-lzzi4rnz 45 6 tests test NNS cord-258014-lzzi4rnz 45 7 that that WDT cord-258014-lzzi4rnz 45 8 could could MD cord-258014-lzzi4rnz 45 9 monitor monitor VB cord-258014-lzzi4rnz 45 10 genital genital JJ cord-258014-lzzi4rnz 45 11 health health NN cord-258014-lzzi4rnz 45 12 based base VBN cord-258014-lzzi4rnz 45 13 on on IN cord-258014-lzzi4rnz 45 14 the the DT cord-258014-lzzi4rnz 45 15 use use NN cord-258014-lzzi4rnz 45 16 of of IN cord-258014-lzzi4rnz 45 17 vaginal vaginal JJ cord-258014-lzzi4rnz 45 18 secretions secretion NNS cord-258014-lzzi4rnz 45 19 and and CC cord-258014-lzzi4rnz 45 20 even even RB cord-258014-lzzi4rnz 45 21 urine urine NN cord-258014-lzzi4rnz 45 22 could could MD cord-258014-lzzi4rnz 45 23 be be VB cord-258014-lzzi4rnz 45 24 useful useful JJ cord-258014-lzzi4rnz 45 25 . . . cord-258014-lzzi4rnz 46 1 Dysbioses dysbiose NNS cord-258014-lzzi4rnz 46 2 of of IN cord-258014-lzzi4rnz 46 3 the the DT cord-258014-lzzi4rnz 46 4 genital genital JJ cord-258014-lzzi4rnz 46 5 microbiome microbiome NN cord-258014-lzzi4rnz 46 6 result result NN cord-258014-lzzi4rnz 46 7 in in IN cord-258014-lzzi4rnz 46 8 high high JJ cord-258014-lzzi4rnz 46 9 pH ph NN cord-258014-lzzi4rnz 46 10 due due IN cord-258014-lzzi4rnz 46 11 to to IN cord-258014-lzzi4rnz 46 12 the the DT cord-258014-lzzi4rnz 46 13 decrease decrease NN cord-258014-lzzi4rnz 46 14 in in IN cord-258014-lzzi4rnz 46 15 fermenting ferment VBG cord-258014-lzzi4rnz 46 16 Lactobacillus lactobacillus NN cord-258014-lzzi4rnz 46 17 , , , cord-258014-lzzi4rnz 46 18 and and CC cord-258014-lzzi4rnz 46 19 create create VB cord-258014-lzzi4rnz 46 20 a a DT cord-258014-lzzi4rnz 46 21 genital genital JJ cord-258014-lzzi4rnz 46 22 microbiome microbiome NN cord-258014-lzzi4rnz 46 23 / / SYM cord-258014-lzzi4rnz 46 24 metabolome metabolome NN cord-258014-lzzi4rnz 46 25 fingerprint fingerprint NN cord-258014-lzzi4rnz 46 26 , , , cord-258014-lzzi4rnz 46 27 which which WDT cord-258014-lzzi4rnz 46 28 in in IN cord-258014-lzzi4rnz 46 29 the the DT cord-258014-lzzi4rnz 46 30 future future NN cord-258014-lzzi4rnz 46 31 could could MD cord-258014-lzzi4rnz 46 32 be be VB cord-258014-lzzi4rnz 46 33 detected detect VBN cord-258014-lzzi4rnz 46 34 with with IN cord-258014-lzzi4rnz 46 35 different different JJ cord-258014-lzzi4rnz 46 36 applications application NNS cord-258014-lzzi4rnz 46 37 [ [ -LRB- cord-258014-lzzi4rnz 46 38 21 21 CD cord-258014-lzzi4rnz 46 39 , , , cord-258014-lzzi4rnz 46 40 22 22 CD cord-258014-lzzi4rnz 46 41 ] ] -RRB- cord-258014-lzzi4rnz 46 42 . . . cord-258014-lzzi4rnz 47 1 These these DT cord-258014-lzzi4rnz 47 2 metabolomic metabolomic JJ cord-258014-lzzi4rnz 47 3 profiles profile NNS cord-258014-lzzi4rnz 47 4 require require VBP cord-258014-lzzi4rnz 47 5 advanced advanced JJ cord-258014-lzzi4rnz 47 6 diagnostics diagnostic NNS cord-258014-lzzi4rnz 47 7 and and CC cord-258014-lzzi4rnz 47 8 point point NN cord-258014-lzzi4rnz 47 9 - - HYPH cord-258014-lzzi4rnz 47 10 of of IN cord-258014-lzzi4rnz 47 11 - - HYPH cord-258014-lzzi4rnz 47 12 care care NN cord-258014-lzzi4rnz 47 13 applications application NNS cord-258014-lzzi4rnz 47 14 for for IN cord-258014-lzzi4rnz 47 15 which which WDT cord-258014-lzzi4rnz 47 16 big big JJ cord-258014-lzzi4rnz 47 17 data datum NNS cord-258014-lzzi4rnz 47 18 from from IN cord-258014-lzzi4rnz 47 19 microbiome microbiome NN cord-258014-lzzi4rnz 47 20 and and CC cord-258014-lzzi4rnz 47 21 metabolome metabolome NNP cord-258014-lzzi4rnz 47 22 are be VBP cord-258014-lzzi4rnz 47 23 needed need VBN cord-258014-lzzi4rnz 47 24 . . . cord-258014-lzzi4rnz 48 1 Here here RB cord-258014-lzzi4rnz 48 2 , , , cord-258014-lzzi4rnz 48 3 we -PRON- PRP cord-258014-lzzi4rnz 48 4 provide provide VBP cord-258014-lzzi4rnz 48 5 a a DT cord-258014-lzzi4rnz 48 6 unique unique JJ cord-258014-lzzi4rnz 48 7 and and CC cord-258014-lzzi4rnz 48 8 detailed detailed JJ cord-258014-lzzi4rnz 48 9 guide guide NN cord-258014-lzzi4rnz 48 10 for for IN cord-258014-lzzi4rnz 48 11 the the DT cord-258014-lzzi4rnz 48 12 development development NN cord-258014-lzzi4rnz 48 13 of of IN cord-258014-lzzi4rnz 48 14 multi multi JJ cord-258014-lzzi4rnz 48 15 - - JJ cord-258014-lzzi4rnz 48 16 omic omic JJ cord-258014-lzzi4rnz 48 17 integration integration NN cord-258014-lzzi4rnz 48 18 studies study NNS cord-258014-lzzi4rnz 48 19 of of IN cord-258014-lzzi4rnz 48 20 the the DT cord-258014-lzzi4rnz 48 21 bacterial bacterial JJ cord-258014-lzzi4rnz 48 22 microbiota microbiota NN cord-258014-lzzi4rnz 48 23 and and CC cord-258014-lzzi4rnz 48 24 urine urine NN cord-258014-lzzi4rnz 48 25 metabolomics metabolomic NNS cord-258014-lzzi4rnz 48 26 to to TO cord-258014-lzzi4rnz 48 27 understand understand VB cord-258014-lzzi4rnz 48 28 possible possible JJ cord-258014-lzzi4rnz 48 29 biological biological JJ cord-258014-lzzi4rnz 48 30 associations association NNS cord-258014-lzzi4rnz 48 31 with with IN cord-258014-lzzi4rnz 48 32 the the DT cord-258014-lzzi4rnz 48 33 host host NN cord-258014-lzzi4rnz 48 34 's 's POS cord-258014-lzzi4rnz 48 35 urinary urinary JJ cord-258014-lzzi4rnz 48 36 metabolites metabolite NNS cord-258014-lzzi4rnz 48 37 . . . cord-258014-lzzi4rnz 49 1 This this DT cord-258014-lzzi4rnz 49 2 article article NN cord-258014-lzzi4rnz 49 3 discloses disclose VBZ cord-258014-lzzi4rnz 49 4 detailed detailed JJ cord-258014-lzzi4rnz 49 5 materials material NNS cord-258014-lzzi4rnz 49 6 , , , cord-258014-lzzi4rnz 49 7 methods method NNS cord-258014-lzzi4rnz 49 8 , , , cord-258014-lzzi4rnz 49 9 sample sample NN cord-258014-lzzi4rnz 49 10 processing processing NN cord-258014-lzzi4rnz 49 11 , , , cord-258014-lzzi4rnz 49 12 and and CC cord-258014-lzzi4rnz 49 13 data data NN cord-258014-lzzi4rnz 49 14 analyses analysis NNS cord-258014-lzzi4rnz 49 15 . . . cord-258014-lzzi4rnz 50 1 The the DT cord-258014-lzzi4rnz 50 2 coupling coupling NN cord-258014-lzzi4rnz 50 3 of of IN cord-258014-lzzi4rnz 50 4 two two CD cord-258014-lzzi4rnz 50 5 omic omic JJ cord-258014-lzzi4rnz 50 6 methods method NNS cord-258014-lzzi4rnz 50 7 is be VBZ cord-258014-lzzi4rnz 50 8 a a DT cord-258014-lzzi4rnz 50 9 useful useful JJ cord-258014-lzzi4rnz 50 10 approach approach NN cord-258014-lzzi4rnz 50 11 to to TO cord-258014-lzzi4rnz 50 12 understand understand VB cord-258014-lzzi4rnz 50 13 the the DT cord-258014-lzzi4rnz 50 14 association association NN cord-258014-lzzi4rnz 50 15 of of IN cord-258014-lzzi4rnz 50 16 the the DT cord-258014-lzzi4rnz 50 17 human human JJ cord-258014-lzzi4rnz 50 18 microbiome microbiome NN cord-258014-lzzi4rnz 50 19 with with IN cord-258014-lzzi4rnz 50 20 health health NN cord-258014-lzzi4rnz 50 21 status status NN cord-258014-lzzi4rnz 50 22 and and CC cord-258014-lzzi4rnz 50 23 risk risk NN cord-258014-lzzi4rnz 50 24 of of IN cord-258014-lzzi4rnz 50 25 disease disease NN cord-258014-lzzi4rnz 50 26 severity severity NN cord-258014-lzzi4rnz 50 27 , , , cord-258014-lzzi4rnz 50 28 and and CC cord-258014-lzzi4rnz 50 29 for for IN cord-258014-lzzi4rnz 50 30 the the DT cord-258014-lzzi4rnz 50 31 development development NN cord-258014-lzzi4rnz 50 32 of of IN cord-258014-lzzi4rnz 50 33 new new JJ cord-258014-lzzi4rnz 50 34 point point NN cord-258014-lzzi4rnz 50 35 - - HYPH cord-258014-lzzi4rnz 50 36 of of IN cord-258014-lzzi4rnz 50 37 - - HYPH cord-258014-lzzi4rnz 50 38 care care NN cord-258014-lzzi4rnz 50 39 testing testing NN cord-258014-lzzi4rnz 50 40 technologies technology NNS cord-258014-lzzi4rnz 50 41 . . . cord-258014-lzzi4rnz 51 1 In in IN cord-258014-lzzi4rnz 51 2 the the DT cord-258014-lzzi4rnz 51 3 Second second JJ cord-258014-lzzi4rnz 51 4 step step NN cord-258014-lzzi4rnz 51 5 ( ( -LRB- cord-258014-lzzi4rnz 51 6 solution solution NN cord-258014-lzzi4rnz 51 7 C2 C2 NNP cord-258014-lzzi4rnz 51 8 ) ) -RRB- cord-258014-lzzi4rnz 51 9 , , , cord-258014-lzzi4rnz 51 10 what what WP cord-258014-lzzi4rnz 51 11 occurs occur VBZ cord-258014-lzzi4rnz 51 12 is be VBZ cord-258014-lzzi4rnz 51 13 the the DT cord-258014-lzzi4rnz 51 14 precipitation precipitation NN cord-258014-lzzi4rnz 51 15 of of IN cord-258014-lzzi4rnz 51 16 non non JJ cord-258014-lzzi4rnz 51 17 - - JJ cord-258014-lzzi4rnz 51 18 DNA dna JJ cord-258014-lzzi4rnz 51 19 organic organic JJ cord-258014-lzzi4rnz 51 20 and and CC cord-258014-lzzi4rnz 51 21 inorganic inorganic JJ cord-258014-lzzi4rnz 51 22 material material NN cord-258014-lzzi4rnz 51 23 , , , cord-258014-lzzi4rnz 51 24 including include VBG cord-258014-lzzi4rnz 51 25 cell cell NN cord-258014-lzzi4rnz 51 26 debris debris NN cord-258014-lzzi4rnz 51 27 and and CC cord-258014-lzzi4rnz 51 28 proteins protein NNS cord-258014-lzzi4rnz 51 29 . . . cord-258014-lzzi4rnz 52 1 Given give VBN cord-258014-lzzi4rnz 52 2 that that IN cord-258014-lzzi4rnz 52 3 cervical cervical JJ cord-258014-lzzi4rnz 52 4 swabs swab NNS cord-258014-lzzi4rnz 52 5 are be VBP cord-258014-lzzi4rnz 52 6 low low JJ cord-258014-lzzi4rnz 52 7 biomass biomass NN cord-258014-lzzi4rnz 52 8 samples sample NNS cord-258014-lzzi4rnz 52 9 , , , cord-258014-lzzi4rnz 52 10 this this DT cord-258014-lzzi4rnz 52 11 step step NN cord-258014-lzzi4rnz 52 12 will will MD cord-258014-lzzi4rnz 52 13 be be VB cord-258014-lzzi4rnz 52 14 joined join VBN cord-258014-lzzi4rnz 52 15 with with IN cord-258014-lzzi4rnz 52 16 inhibitor inhibitor NN cord-258014-lzzi4rnz 52 17 removal removal NN cord-258014-lzzi4rnz 52 18 ( ( -LRB- cord-258014-lzzi4rnz 52 19 solution solution NN cord-258014-lzzi4rnz 52 20 C3 C3 NNP cord-258014-lzzi4rnz 52 21 ) ) -RRB- cord-258014-lzzi4rnz 52 22 . . . cord-258014-lzzi4rnz 53 1 8 8 CD cord-258014-lzzi4rnz 53 2 . . . cord-258014-lzzi4rnz 54 1 Add add VB cord-258014-lzzi4rnz 54 2 100 100 CD cord-258014-lzzi4rnz 54 3 µL µl CD cord-258014-lzzi4rnz 54 4 of of IN cord-258014-lzzi4rnz 54 5 C2 C2 NNP cord-258014-lzzi4rnz 54 6 and and CC cord-258014-lzzi4rnz 54 7 100 100 CD cord-258014-lzzi4rnz 54 8 µL µl CD cord-258014-lzzi4rnz 54 9 of of IN cord-258014-lzzi4rnz 54 10 C3 c3 NN cord-258014-lzzi4rnz 54 11 . . . cord-258014-lzzi4rnz 55 1 Vortex vortex NN cord-258014-lzzi4rnz 55 2 for for IN cord-258014-lzzi4rnz 55 3 5 5 CD cord-258014-lzzi4rnz 55 4 s. s. NN cord-258014-lzzi4rnz 55 5 9 9 CD cord-258014-lzzi4rnz 55 6 . . . cord-258014-lzzi4rnz 56 1 Incubate incubate VB cord-258014-lzzi4rnz 56 2 at at IN cord-258014-lzzi4rnz 56 3 −20 −20 NNP cord-258014-lzzi4rnz 56 4 • • XX cord-258014-lzzi4rnz 56 5 C C NNP cord-258014-lzzi4rnz 56 6 for for IN cord-258014-lzzi4rnz 56 7 5 5 CD cord-258014-lzzi4rnz 56 8 min min NN cord-258014-lzzi4rnz 56 9 . . . cord-258014-lzzi4rnz 57 1 10 10 CD cord-258014-lzzi4rnz 57 2 . . . cord-258014-lzzi4rnz 58 1 Centrifuge centrifuge NN cord-258014-lzzi4rnz 58 2 at at IN cord-258014-lzzi4rnz 58 3 room room NN cord-258014-lzzi4rnz 58 4 temperature temperature NN cord-258014-lzzi4rnz 58 5 for for IN cord-258014-lzzi4rnz 58 6 1 1 CD cord-258014-lzzi4rnz 58 7 min min NN cord-258014-lzzi4rnz 58 8 at at IN cord-258014-lzzi4rnz 58 9 13,000 13,000 CD cord-258014-lzzi4rnz 58 10 rpm rpm NN cord-258014-lzzi4rnz 58 11 . . . cord-258014-lzzi4rnz 59 1 11 11 CD cord-258014-lzzi4rnz 59 2 . . . cord-258014-lzzi4rnz 60 1 Avoiding avoid VBG cord-258014-lzzi4rnz 60 2 the the DT cord-258014-lzzi4rnz 60 3 pellet pellet NN cord-258014-lzzi4rnz 60 4 , , , cord-258014-lzzi4rnz 60 5 transfer transfer VB cord-258014-lzzi4rnz 60 6 up up RP cord-258014-lzzi4rnz 60 7 to to IN cord-258014-lzzi4rnz 60 8 , , , cord-258014-lzzi4rnz 60 9 but but CC cord-258014-lzzi4rnz 60 10 no no DT cord-258014-lzzi4rnz 60 11 more more JJR cord-258014-lzzi4rnz 60 12 than than IN cord-258014-lzzi4rnz 60 13 750 750 CD cord-258014-lzzi4rnz 60 14 µL µl CD cord-258014-lzzi4rnz 60 15 of of IN cord-258014-lzzi4rnz 60 16 supernatant supernatant NN cord-258014-lzzi4rnz 60 17 to to IN cord-258014-lzzi4rnz 60 18 a a DT cord-258014-lzzi4rnz 60 19 clean clean JJ cord-258014-lzzi4rnz 60 20 2 2 CD cord-258014-lzzi4rnz 60 21 mL ml NN cord-258014-lzzi4rnz 60 22 tube tube NN cord-258014-lzzi4rnz 60 23 . . . cord-258014-lzzi4rnz 61 1 12 12 CD cord-258014-lzzi4rnz 61 2 . . . cord-258014-lzzi4rnz 62 1 The the DT cord-258014-lzzi4rnz 62 2 next next JJ cord-258014-lzzi4rnz 62 3 step step NN cord-258014-lzzi4rnz 62 4 with with IN cord-258014-lzzi4rnz 62 5 solution solution NN cord-258014-lzzi4rnz 62 6 4 4 CD cord-258014-lzzi4rnz 62 7 ( ( -LRB- cord-258014-lzzi4rnz 62 8 rich rich JJ cord-258014-lzzi4rnz 62 9 in in IN cord-258014-lzzi4rnz 62 10 salt salt NN cord-258014-lzzi4rnz 62 11 ) ) -RRB- cord-258014-lzzi4rnz 62 12 , , , cord-258014-lzzi4rnz 62 13 is be VBZ cord-258014-lzzi4rnz 62 14 aimed aim VBN cord-258014-lzzi4rnz 62 15 at at IN cord-258014-lzzi4rnz 62 16 binding bind VBG cord-258014-lzzi4rnz 62 17 the the DT cord-258014-lzzi4rnz 62 18 DNA dna NN cord-258014-lzzi4rnz 62 19 to to TO cord-258014-lzzi4rnz 62 20 spin spin VB cord-258014-lzzi4rnz 62 21 filter filter NN cord-258014-lzzi4rnz 62 22 . . . cord-258014-lzzi4rnz 63 1 13 13 CD cord-258014-lzzi4rnz 63 2 . . . cord-258014-lzzi4rnz 64 1 Add add VB cord-258014-lzzi4rnz 64 2 1200 1200 CD cord-258014-lzzi4rnz 64 3 µL µl CD cord-258014-lzzi4rnz 64 4 of of IN cord-258014-lzzi4rnz 64 5 C4 C4 NNP cord-258014-lzzi4rnz 64 6 ( ( -LRB- cord-258014-lzzi4rnz 64 7 previously previously RB cord-258014-lzzi4rnz 64 8 shaken shake VBN cord-258014-lzzi4rnz 64 9 ) ) -RRB- cord-258014-lzzi4rnz 64 10 to to IN cord-258014-lzzi4rnz 64 11 the the DT cord-258014-lzzi4rnz 64 12 former former JJ cord-258014-lzzi4rnz 64 13 supernatant supernatant NN cord-258014-lzzi4rnz 64 14 and and CC cord-258014-lzzi4rnz 64 15 vortex vortex NN cord-258014-lzzi4rnz 64 16 for for IN cord-258014-lzzi4rnz 64 17 5 5 CD cord-258014-lzzi4rnz 64 18 s. s. NNP cord-258014-lzzi4rnz 64 19 14 14 CD cord-258014-lzzi4rnz 64 20 . . . cord-258014-lzzi4rnz 65 1 Place place VB cord-258014-lzzi4rnz 65 2 the the DT cord-258014-lzzi4rnz 65 3 spin spin NN cord-258014-lzzi4rnz 65 4 filters filter NNS cord-258014-lzzi4rnz 65 5 in in IN cord-258014-lzzi4rnz 65 6 the the DT cord-258014-lzzi4rnz 65 7 vacuum vacuum NN cord-258014-lzzi4rnz 65 8 manifold manifold NN cord-258014-lzzi4rnz 65 9 and and CC cord-258014-lzzi4rnz 65 10 load load VBP cord-258014-lzzi4rnz 65 11 approximately approximately RB cord-258014-lzzi4rnz 65 12 675 675 CD cord-258014-lzzi4rnz 65 13 µL µl CD cord-258014-lzzi4rnz 65 14 onto onto IN cord-258014-lzzi4rnz 65 15 a a DT cord-258014-lzzi4rnz 65 16 spin spin NN cord-258014-lzzi4rnz 65 17 filter filter NN cord-258014-lzzi4rnz 65 18 . . . cord-258014-lzzi4rnz 66 1 Turn turn VB cord-258014-lzzi4rnz 66 2 on on IN cord-258014-lzzi4rnz 66 3 the the DT cord-258014-lzzi4rnz 66 4 vacuum vacuum NN cord-258014-lzzi4rnz 66 5 until until IN cord-258014-lzzi4rnz 66 6 all all PDT cord-258014-lzzi4rnz 66 7 the the DT cord-258014-lzzi4rnz 66 8 supernatant supernatant JJ cord-258014-lzzi4rnz 66 9 flows flow VBZ cord-258014-lzzi4rnz 66 10 through through RB cord-258014-lzzi4rnz 66 11 . . . cord-258014-lzzi4rnz 67 1 Add add VB cord-258014-lzzi4rnz 67 2 an an DT cord-258014-lzzi4rnz 67 3 additional additional JJ cord-258014-lzzi4rnz 67 4 675 675 CD cord-258014-lzzi4rnz 67 5 µL µl CD cord-258014-lzzi4rnz 67 6 of of IN cord-258014-lzzi4rnz 67 7 the the DT cord-258014-lzzi4rnz 67 8 supernatant supernatant NN cord-258014-lzzi4rnz 67 9 to to IN cord-258014-lzzi4rnz 67 10 the the DT cord-258014-lzzi4rnz 67 11 spin spin NN cord-258014-lzzi4rnz 67 12 filter filter NN cord-258014-lzzi4rnz 67 13 and and CC cord-258014-lzzi4rnz 67 14 vacuum vacuum NN cord-258014-lzzi4rnz 67 15 . . . cord-258014-lzzi4rnz 68 1 15 15 CD cord-258014-lzzi4rnz 68 2 . . . cord-258014-lzzi4rnz 69 1 Load load VB cord-258014-lzzi4rnz 69 2 the the DT cord-258014-lzzi4rnz 69 3 remaining remain VBG cord-258014-lzzi4rnz 69 4 of of IN cord-258014-lzzi4rnz 69 5 the the DT cord-258014-lzzi4rnz 69 6 supernatant supernatant NN cord-258014-lzzi4rnz 69 7 and and CC cord-258014-lzzi4rnz 69 8 repeat repeat VB cord-258014-lzzi4rnz 69 9 it -PRON- PRP cord-258014-lzzi4rnz 69 10 . . . cord-258014-lzzi4rnz 70 1 A a DT cord-258014-lzzi4rnz 70 2 total total NN cord-258014-lzzi4rnz 70 3 of of IN cord-258014-lzzi4rnz 70 4 three three CD cord-258014-lzzi4rnz 70 5 loads load NNS cord-258014-lzzi4rnz 70 6 for for IN cord-258014-lzzi4rnz 70 7 each each DT cord-258014-lzzi4rnz 70 8 sample sample NN cord-258014-lzzi4rnz 70 9 processed process VBN cord-258014-lzzi4rnz 70 10 are be VBP cord-258014-lzzi4rnz 70 11 required require VBN cord-258014-lzzi4rnz 70 12 . . . cord-258014-lzzi4rnz 71 1 16 16 CD cord-258014-lzzi4rnz 71 2 . . . cord-258014-lzzi4rnz 72 1 The the DT cord-258014-lzzi4rnz 72 2 next next JJ cord-258014-lzzi4rnz 72 3 step step NN cord-258014-lzzi4rnz 72 4 includes include VBZ cord-258014-lzzi4rnz 72 5 washing wash VBG cord-258014-lzzi4rnz 72 6 the the DT cord-258014-lzzi4rnz 72 7 DNA dna NN cord-258014-lzzi4rnz 72 8 bound bind VBN cord-258014-lzzi4rnz 72 9 to to IN cord-258014-lzzi4rnz 72 10 the the DT cord-258014-lzzi4rnz 72 11 membrane membrane NN cord-258014-lzzi4rnz 72 12 and and CC cord-258014-lzzi4rnz 72 13 remove remove VB cord-258014-lzzi4rnz 72 14 contaminants contaminant NNS cord-258014-lzzi4rnz 72 15 . . . cord-258014-lzzi4rnz 73 1 17 17 CD cord-258014-lzzi4rnz 73 2 . . . cord-258014-lzzi4rnz 74 1 Add add VB cord-258014-lzzi4rnz 74 2 650 650 CD cord-258014-lzzi4rnz 74 3 µL µl CD cord-258014-lzzi4rnz 74 4 of of IN cord-258014-lzzi4rnz 74 5 cold cold JJ cord-258014-lzzi4rnz 74 6 100 100 CD cord-258014-lzzi4rnz 74 7 % % NN cord-258014-lzzi4rnz 74 8 ethanol ethanol NN cord-258014-lzzi4rnz 74 9 ( ( -LRB- cord-258014-lzzi4rnz 74 10 −20 −20 NNP cord-258014-lzzi4rnz 74 11 • • NNP cord-258014-lzzi4rnz 74 12 C C NNP cord-258014-lzzi4rnz 74 13 ) ) -RRB- cord-258014-lzzi4rnz 74 14 to to IN cord-258014-lzzi4rnz 74 15 all all PDT cord-258014-lzzi4rnz 74 16 the the DT cord-258014-lzzi4rnz 74 17 tubes tube NNS cord-258014-lzzi4rnz 74 18 and and CC cord-258014-lzzi4rnz 74 19 turn turn VB cord-258014-lzzi4rnz 74 20 on on RP cord-258014-lzzi4rnz 74 21 the the DT cord-258014-lzzi4rnz 74 22 vacuum vacuum NN cord-258014-lzzi4rnz 74 23 . . . cord-258014-lzzi4rnz 75 1 18 18 CD cord-258014-lzzi4rnz 75 2 . . . cord-258014-lzzi4rnz 76 1 Add add VB cord-258014-lzzi4rnz 76 2 500 500 CD cord-258014-lzzi4rnz 76 3 µL µl CD cord-258014-lzzi4rnz 76 4 of of IN cord-258014-lzzi4rnz 76 5 solution solution NN cord-258014-lzzi4rnz 76 6 C5 c5 NN cord-258014-lzzi4rnz 76 7 to to IN cord-258014-lzzi4rnz 76 8 all all DT cord-258014-lzzi4rnz 76 9 tubes tube NNS cord-258014-lzzi4rnz 76 10 and and CC cord-258014-lzzi4rnz 76 11 turn turn VB cord-258014-lzzi4rnz 76 12 on on RP cord-258014-lzzi4rnz 76 13 the the DT cord-258014-lzzi4rnz 76 14 vacuum vacuum NN cord-258014-lzzi4rnz 76 15 . . . cord-258014-lzzi4rnz 77 1 Carefully carefully RB cord-258014-lzzi4rnz 77 2 place place VB cord-258014-lzzi4rnz 77 3 the the DT cord-258014-lzzi4rnz 77 4 spin spin NN cord-258014-lzzi4rnz 77 5 filter filter NN cord-258014-lzzi4rnz 77 6 in in IN cord-258014-lzzi4rnz 77 7 a a DT cord-258014-lzzi4rnz 77 8 clean clean JJ cord-258014-lzzi4rnz 77 9 2ml 2ml JJ cord-258014-lzzi4rnz 77 10 collection collection NN cord-258014-lzzi4rnz 77 11 tube tube NN cord-258014-lzzi4rnz 77 12 . . . cord-258014-lzzi4rnz 78 1 Avoid avoid VB cord-258014-lzzi4rnz 78 2 splashing splash VBG cord-258014-lzzi4rnz 78 3 any any DT cord-258014-lzzi4rnz 78 4 Solution Solution NNP cord-258014-lzzi4rnz 78 5 C5 C5 NNP cord-258014-lzzi4rnz 78 6 onto onto IN cord-258014-lzzi4rnz 78 7 the the DT cord-258014-lzzi4rnz 78 8 spin spin NN cord-258014-lzzi4rnz 78 9 filter filter NN cord-258014-lzzi4rnz 78 10 . . . cord-258014-lzzi4rnz 79 1 It -PRON- PRP cord-258014-lzzi4rnz 79 2 is be VBZ cord-258014-lzzi4rnz 79 3 critical critical JJ cord-258014-lzzi4rnz 79 4 to to TO cord-258014-lzzi4rnz 79 5 remove remove VB cord-258014-lzzi4rnz 79 6 all all DT cord-258014-lzzi4rnz 79 7 traces trace NNS cord-258014-lzzi4rnz 79 8 of of IN cord-258014-lzzi4rnz 79 9 Solution Solution NNP cord-258014-lzzi4rnz 79 10 C5 C5 NNP cord-258014-lzzi4rnz 79 11 as as IN cord-258014-lzzi4rnz 79 12 it -PRON- PRP cord-258014-lzzi4rnz 79 13 can can MD cord-258014-lzzi4rnz 79 14 interfere interfere VB cord-258014-lzzi4rnz 79 15 with with IN cord-258014-lzzi4rnz 79 16 many many JJ cord-258014-lzzi4rnz 79 17 downstream downstream JJ cord-258014-lzzi4rnz 79 18 DNA dna NN cord-258014-lzzi4rnz 79 19 applications application NNS cord-258014-lzzi4rnz 79 20 . . . cord-258014-lzzi4rnz 80 1 19 19 CD cord-258014-lzzi4rnz 80 2 . . . cord-258014-lzzi4rnz 81 1 The the DT cord-258014-lzzi4rnz 81 2 final final JJ cord-258014-lzzi4rnz 81 3 step step NN cord-258014-lzzi4rnz 81 4 is be VBZ cord-258014-lzzi4rnz 81 5 DNA DNA NNP cord-258014-lzzi4rnz 81 6 elution elution NN cord-258014-lzzi4rnz 81 7 from from IN cord-258014-lzzi4rnz 81 8 the the DT cord-258014-lzzi4rnz 81 9 membrane membrane NN cord-258014-lzzi4rnz 81 10 using use VBG cord-258014-lzzi4rnz 81 11 sterile sterile JJ cord-258014-lzzi4rnz 81 12 solution solution NN cord-258014-lzzi4rnz 81 13 C6 c6 NN cord-258014-lzzi4rnz 81 14 ( ( -LRB- cord-258014-lzzi4rnz 81 15 10 10 CD cord-258014-lzzi4rnz 81 16 mM mm CD cord-258014-lzzi4rnz 81 17 Tris Tris NNP cord-258014-lzzi4rnz 81 18 ) ) -RRB- cord-258014-lzzi4rnz 81 19 or or CC cord-258014-lzzi4rnz 81 20 sterile sterile JJ cord-258014-lzzi4rnz 81 21 PCR PCR NNP cord-258014-lzzi4rnz 81 22 water water NN cord-258014-lzzi4rnz 81 23 . . . cord-258014-lzzi4rnz 82 1 20 20 CD cord-258014-lzzi4rnz 82 2 . . . cord-258014-lzzi4rnz 83 1 Add add VB cord-258014-lzzi4rnz 83 2 60 60 CD cord-258014-lzzi4rnz 83 3 - - SYM cord-258014-lzzi4rnz 83 4 100 100 CD cord-258014-lzzi4rnz 83 5 µL µl CD cord-258014-lzzi4rnz 83 6 of of IN cord-258014-lzzi4rnz 83 7 C6 C6 NNP cord-258014-lzzi4rnz 83 8 ( ( -LRB- cord-258014-lzzi4rnz 83 9 55 55 CD cord-258014-lzzi4rnz 83 10 • • NN cord-258014-lzzi4rnz 83 11 C c NN cord-258014-lzzi4rnz 83 12 ) ) -RRB- cord-258014-lzzi4rnz 83 13 to to IN cord-258014-lzzi4rnz 83 14 the the DT cord-258014-lzzi4rnz 83 15 center center NN cord-258014-lzzi4rnz 83 16 of of IN cord-258014-lzzi4rnz 83 17 the the DT cord-258014-lzzi4rnz 83 18 white white JJ cord-258014-lzzi4rnz 83 19 filter filter NN cord-258014-lzzi4rnz 83 20 membrane membrane NN cord-258014-lzzi4rnz 83 21 and and CC cord-258014-lzzi4rnz 83 22 let let VB cord-258014-lzzi4rnz 83 23 sit sit VB cord-258014-lzzi4rnz 83 24 for for IN cord-258014-lzzi4rnz 83 25 5 5 CD cord-258014-lzzi4rnz 83 26 min min NN cord-258014-lzzi4rnz 83 27 . . . cord-258014-lzzi4rnz 84 1 Volume volume NN cord-258014-lzzi4rnz 84 2 range range NN cord-258014-lzzi4rnz 84 3 depends depend VBZ cord-258014-lzzi4rnz 84 4 on on IN cord-258014-lzzi4rnz 84 5 how how WRB cord-258014-lzzi4rnz 84 6 diluted dilute VBN cord-258014-lzzi4rnz 84 7 you -PRON- PRP cord-258014-lzzi4rnz 84 8 wish wish VBP cord-258014-lzzi4rnz 84 9 the the DT cord-258014-lzzi4rnz 84 10 sample sample NN cord-258014-lzzi4rnz 84 11 to to TO cord-258014-lzzi4rnz 84 12 be be VB cord-258014-lzzi4rnz 84 13 , , , cord-258014-lzzi4rnz 84 14 If if IN cord-258014-lzzi4rnz 84 15 low low JJ cord-258014-lzzi4rnz 84 16 biomass biomass NN cord-258014-lzzi4rnz 84 17 , , , cord-258014-lzzi4rnz 84 18 lower low JJR cord-258014-lzzi4rnz 84 19 volumes volume NNS cord-258014-lzzi4rnz 84 20 of of IN cord-258014-lzzi4rnz 84 21 C6 C6 NNP cord-258014-lzzi4rnz 84 22 are be VBP cord-258014-lzzi4rnz 84 23 suggested suggest VBN cord-258014-lzzi4rnz 84 24 . . . cord-258014-lzzi4rnz 85 1 21 21 CD cord-258014-lzzi4rnz 85 2 . . . cord-258014-lzzi4rnz 86 1 Centrifuge Centrifuge NNP cord-258014-lzzi4rnz 86 2 for for IN cord-258014-lzzi4rnz 86 3 1 1 CD cord-258014-lzzi4rnz 86 4 min min NN cord-258014-lzzi4rnz 86 5 at at IN cord-258014-lzzi4rnz 86 6 room room NN cord-258014-lzzi4rnz 86 7 temperature temperature NN cord-258014-lzzi4rnz 86 8 at at IN cord-258014-lzzi4rnz 86 9 13,000 13,000 CD cord-258014-lzzi4rnz 86 10 rpm rpm NN cord-258014-lzzi4rnz 86 11 . . . cord-258014-lzzi4rnz 87 1 22 22 CD cord-258014-lzzi4rnz 87 2 . . . cord-258014-lzzi4rnz 88 1 Discard discard VB cord-258014-lzzi4rnz 88 2 the the DT cord-258014-lzzi4rnz 88 3 spin spin NN cord-258014-lzzi4rnz 88 4 filter filter NN cord-258014-lzzi4rnz 88 5 and and CC cord-258014-lzzi4rnz 88 6 store store NN cord-258014-lzzi4rnz 88 7 DNA dna NN cord-258014-lzzi4rnz 88 8 at at IN cord-258014-lzzi4rnz 88 9 −20 −20 NNP cord-258014-lzzi4rnz 88 10 • • XX cord-258014-lzzi4rnz 88 11 C C NNP cord-258014-lzzi4rnz 88 12 ( ( -LRB- cord-258014-lzzi4rnz 88 13 Figure figure NN cord-258014-lzzi4rnz 88 14 1 1 CD cord-258014-lzzi4rnz 88 15 ) ) -RRB- cord-258014-lzzi4rnz 88 16 . . . cord-258014-lzzi4rnz 89 1 7 7 CD cord-258014-lzzi4rnz 89 2 . . . cord-258014-lzzi4rnz 90 1 In in IN cord-258014-lzzi4rnz 90 2 the the DT cord-258014-lzzi4rnz 90 3 Second second JJ cord-258014-lzzi4rnz 90 4 step step NN cord-258014-lzzi4rnz 90 5 ( ( -LRB- cord-258014-lzzi4rnz 90 6 solution solution NN cord-258014-lzzi4rnz 90 7 C2 C2 NNP cord-258014-lzzi4rnz 90 8 ) ) -RRB- cord-258014-lzzi4rnz 90 9 , , , cord-258014-lzzi4rnz 90 10 what what WP cord-258014-lzzi4rnz 90 11 occurs occur VBZ cord-258014-lzzi4rnz 90 12 is be VBZ cord-258014-lzzi4rnz 90 13 the the DT cord-258014-lzzi4rnz 90 14 precipitation precipitation NN cord-258014-lzzi4rnz 90 15 of of IN cord-258014-lzzi4rnz 90 16 non non JJ cord-258014-lzzi4rnz 90 17 - - JJ cord-258014-lzzi4rnz 90 18 DNA dna JJ cord-258014-lzzi4rnz 90 19 organic organic JJ cord-258014-lzzi4rnz 90 20 and and CC cord-258014-lzzi4rnz 90 21 inorganic inorganic JJ cord-258014-lzzi4rnz 90 22 material material NN cord-258014-lzzi4rnz 90 23 , , , cord-258014-lzzi4rnz 90 24 including include VBG cord-258014-lzzi4rnz 90 25 cell cell NN cord-258014-lzzi4rnz 90 26 debris debris NN cord-258014-lzzi4rnz 90 27 and and CC cord-258014-lzzi4rnz 90 28 proteins protein NNS cord-258014-lzzi4rnz 90 29 . . . cord-258014-lzzi4rnz 91 1 Given give VBN cord-258014-lzzi4rnz 91 2 that that IN cord-258014-lzzi4rnz 91 3 cervical cervical JJ cord-258014-lzzi4rnz 91 4 swabs swab NNS cord-258014-lzzi4rnz 91 5 are be VBP cord-258014-lzzi4rnz 91 6 low low JJ cord-258014-lzzi4rnz 91 7 biomass biomass NN cord-258014-lzzi4rnz 91 8 samples sample NNS cord-258014-lzzi4rnz 91 9 , , , cord-258014-lzzi4rnz 91 10 this this DT cord-258014-lzzi4rnz 91 11 step step NN cord-258014-lzzi4rnz 91 12 will will MD cord-258014-lzzi4rnz 91 13 be be VB cord-258014-lzzi4rnz 91 14 joined join VBN cord-258014-lzzi4rnz 91 15 with with IN cord-258014-lzzi4rnz 91 16 inhibitor inhibitor NN cord-258014-lzzi4rnz 91 17 removal removal NN cord-258014-lzzi4rnz 91 18 ( ( -LRB- cord-258014-lzzi4rnz 91 19 solution solution NN cord-258014-lzzi4rnz 91 20 C3 C3 NNP cord-258014-lzzi4rnz 91 21 ) ) -RRB- cord-258014-lzzi4rnz 91 22 . . . cord-258014-lzzi4rnz 92 1 8 8 CD cord-258014-lzzi4rnz 92 2 . . . cord-258014-lzzi4rnz 93 1 Add add VB cord-258014-lzzi4rnz 93 2 100 100 CD cord-258014-lzzi4rnz 93 3 μL μL NNS cord-258014-lzzi4rnz 93 4 of of IN cord-258014-lzzi4rnz 93 5 C2 C2 NNP cord-258014-lzzi4rnz 93 6 and and CC cord-258014-lzzi4rnz 93 7 100 100 CD cord-258014-lzzi4rnz 93 8 μL μL NNS cord-258014-lzzi4rnz 93 9 of of IN cord-258014-lzzi4rnz 93 10 C3 c3 NN cord-258014-lzzi4rnz 93 11 . . . cord-258014-lzzi4rnz 94 1 Vortex vortex NN cord-258014-lzzi4rnz 94 2 for for IN cord-258014-lzzi4rnz 94 3 5 5 CD cord-258014-lzzi4rnz 94 4 s. s. NN cord-258014-lzzi4rnz 94 5 9 9 CD cord-258014-lzzi4rnz 94 6 . . . cord-258014-lzzi4rnz 95 1 Incubate incubate VB cord-258014-lzzi4rnz 95 2 at at IN cord-258014-lzzi4rnz 95 3 −20 −20 NNP cord-258014-lzzi4rnz 95 4 ° ° NN cord-258014-lzzi4rnz 95 5 C C NNP cord-258014-lzzi4rnz 95 6 for for IN cord-258014-lzzi4rnz 95 7 5 5 CD cord-258014-lzzi4rnz 95 8 min min NN cord-258014-lzzi4rnz 95 9 . . . cord-258014-lzzi4rnz 96 1 10 10 CD cord-258014-lzzi4rnz 96 2 . . . cord-258014-lzzi4rnz 97 1 Centrifuge centrifuge NN cord-258014-lzzi4rnz 97 2 at at IN cord-258014-lzzi4rnz 97 3 room room NN cord-258014-lzzi4rnz 97 4 temperature temperature NN cord-258014-lzzi4rnz 97 5 for for IN cord-258014-lzzi4rnz 97 6 1 1 CD cord-258014-lzzi4rnz 97 7 min min NN cord-258014-lzzi4rnz 97 8 at at IN cord-258014-lzzi4rnz 97 9 13,000 13,000 CD cord-258014-lzzi4rnz 97 10 rpm rpm NN cord-258014-lzzi4rnz 97 11 . . . cord-258014-lzzi4rnz 98 1 11 11 CD cord-258014-lzzi4rnz 98 2 . . . cord-258014-lzzi4rnz 99 1 Avoiding avoid VBG cord-258014-lzzi4rnz 99 2 the the DT cord-258014-lzzi4rnz 99 3 pellet pellet NN cord-258014-lzzi4rnz 99 4 , , , cord-258014-lzzi4rnz 99 5 transfer transfer VB cord-258014-lzzi4rnz 99 6 up up RP cord-258014-lzzi4rnz 99 7 to to IN cord-258014-lzzi4rnz 99 8 , , , cord-258014-lzzi4rnz 99 9 but but CC cord-258014-lzzi4rnz 99 10 no no DT cord-258014-lzzi4rnz 99 11 more more JJR cord-258014-lzzi4rnz 99 12 than than IN cord-258014-lzzi4rnz 99 13 750 750 CD cord-258014-lzzi4rnz 99 14 μL μL NNS cord-258014-lzzi4rnz 99 15 of of IN cord-258014-lzzi4rnz 99 16 supernatant supernatant NN cord-258014-lzzi4rnz 99 17 to to IN cord-258014-lzzi4rnz 99 18 a a DT cord-258014-lzzi4rnz 99 19 clean clean JJ cord-258014-lzzi4rnz 99 20 2 2 CD cord-258014-lzzi4rnz 99 21 mL ml NN cord-258014-lzzi4rnz 99 22 tube tube NN cord-258014-lzzi4rnz 99 23 . . . cord-258014-lzzi4rnz 100 1 12 12 CD cord-258014-lzzi4rnz 100 2 . . . cord-258014-lzzi4rnz 101 1 The the DT cord-258014-lzzi4rnz 101 2 next next JJ cord-258014-lzzi4rnz 101 3 step step NN cord-258014-lzzi4rnz 101 4 with with IN cord-258014-lzzi4rnz 101 5 solution solution NN cord-258014-lzzi4rnz 101 6 4 4 CD cord-258014-lzzi4rnz 101 7 ( ( -LRB- cord-258014-lzzi4rnz 101 8 rich rich JJ cord-258014-lzzi4rnz 101 9 in in IN cord-258014-lzzi4rnz 101 10 salt salt NN cord-258014-lzzi4rnz 101 11 ) ) -RRB- cord-258014-lzzi4rnz 101 12 , , , cord-258014-lzzi4rnz 101 13 is be VBZ cord-258014-lzzi4rnz 101 14 aimed aim VBN cord-258014-lzzi4rnz 101 15 at at IN cord-258014-lzzi4rnz 101 16 binding bind VBG cord-258014-lzzi4rnz 101 17 the the DT cord-258014-lzzi4rnz 101 18 DNA dna NN cord-258014-lzzi4rnz 101 19 to to TO cord-258014-lzzi4rnz 101 20 spin spin VB cord-258014-lzzi4rnz 101 21 filter filter NN cord-258014-lzzi4rnz 101 22 . . . cord-258014-lzzi4rnz 102 1 13 13 CD cord-258014-lzzi4rnz 102 2 . . . cord-258014-lzzi4rnz 103 1 Add add VB cord-258014-lzzi4rnz 103 2 1200 1200 CD cord-258014-lzzi4rnz 103 3 μL μl NN cord-258014-lzzi4rnz 103 4 of of IN cord-258014-lzzi4rnz 103 5 C4 c4 NN cord-258014-lzzi4rnz 103 6 ( ( -LRB- cord-258014-lzzi4rnz 103 7 previously previously RB cord-258014-lzzi4rnz 103 8 shaken shake VBN cord-258014-lzzi4rnz 103 9 ) ) -RRB- cord-258014-lzzi4rnz 103 10 to to IN cord-258014-lzzi4rnz 103 11 the the DT cord-258014-lzzi4rnz 103 12 former former JJ cord-258014-lzzi4rnz 103 13 supernatant supernatant NN cord-258014-lzzi4rnz 103 14 and and CC cord-258014-lzzi4rnz 103 15 vortex vortex NN cord-258014-lzzi4rnz 103 16 for for IN cord-258014-lzzi4rnz 103 17 5 5 CD cord-258014-lzzi4rnz 103 18 s. s. NNP cord-258014-lzzi4rnz 103 19 _SP cord-258014-lzzi4rnz 104 1 We -PRON- PRP cord-258014-lzzi4rnz 104 2 suggest suggest VBP cord-258014-lzzi4rnz 104 3 the the DT cord-258014-lzzi4rnz 104 4 use use NN cord-258014-lzzi4rnz 104 5 of of IN cord-258014-lzzi4rnz 104 6 a a DT cord-258014-lzzi4rnz 104 7 highly highly RB cord-258014-lzzi4rnz 104 8 sensitive sensitive JJ cord-258014-lzzi4rnz 104 9 short short JJ cord-258014-lzzi4rnz 104 10 - - HYPH cord-258014-lzzi4rnz 104 11 polymerase polymerase NN cord-258014-lzzi4rnz 104 12 chain chain NN cord-258014-lzzi4rnz 104 13 reaction reaction NN cord-258014-lzzi4rnz 104 14 - - HYPH cord-258014-lzzi4rnz 104 15 fragment fragment NN cord-258014-lzzi4rnz 104 16 assay assay NN cord-258014-lzzi4rnz 104 17 ( ( -LRB- cord-258014-lzzi4rnz 104 18 Labo Labo NNP cord-258014-lzzi4rnz 104 19 Biomedical Biomedical NNP cord-258014-lzzi4rnz 104 20 Products Products NNPS cord-258014-lzzi4rnz 104 21 , , , cord-258014-lzzi4rnz 104 22 Rijswijk Rijswijk NNP cord-258014-lzzi4rnz 104 23 , , , cord-258014-lzzi4rnz 104 24 The the DT cord-258014-lzzi4rnz 104 25 Netherlands Netherlands NNP cord-258014-lzzi4rnz 104 26 , , , cord-258014-lzzi4rnz 104 27 licensed licensed JJ cord-258014-lzzi4rnz 104 28 Innogenetics Innogenetics NNP cord-258014-lzzi4rnz 104 29 technology technology NN cord-258014-lzzi4rnz 104 30 ) ) -RRB- cord-258014-lzzi4rnz 104 31 . . . cord-258014-lzzi4rnz 105 1 This this DT cord-258014-lzzi4rnz 105 2 assay assay NN cord-258014-lzzi4rnz 105 3 has have VBZ cord-258014-lzzi4rnz 105 4 been be VBN cord-258014-lzzi4rnz 105 5 used use VBN cord-258014-lzzi4rnz 105 6 for for IN cord-258014-lzzi4rnz 105 7 epidemiological epidemiological JJ cord-258014-lzzi4rnz 105 8 and and CC cord-258014-lzzi4rnz 105 9 vaccination vaccination NN cord-258014-lzzi4rnz 105 10 studies study NNS cord-258014-lzzi4rnz 105 11 because because IN cord-258014-lzzi4rnz 105 12 it -PRON- PRP cord-258014-lzzi4rnz 105 13 has have VBZ cord-258014-lzzi4rnz 105 14 high high JJ cord-258014-lzzi4rnz 105 15 analytical analytical JJ cord-258014-lzzi4rnz 105 16 sensitivity sensitivity NN cord-258014-lzzi4rnz 105 17 [ [ -LRB- cord-258014-lzzi4rnz 105 18 24 24 CD cord-258014-lzzi4rnz 105 19 , , , cord-258014-lzzi4rnz 105 20 25 25 CD cord-258014-lzzi4rnz 105 21 ] ] -RRB- cord-258014-lzzi4rnz 105 22 and and CC cord-258014-lzzi4rnz 105 23 other other JJ cord-258014-lzzi4rnz 105 24 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 105 25 microbiota microbiota NN cord-258014-lzzi4rnz 105 26 related relate VBN cord-258014-lzzi4rnz 105 27 studies study NNS cord-258014-lzzi4rnz 105 28 [ [ -LRB- cord-258014-lzzi4rnz 105 29 5 5 CD cord-258014-lzzi4rnz 105 30 , , , cord-258014-lzzi4rnz 105 31 26 26 CD cord-258014-lzzi4rnz 105 32 ] ] -RRB- cord-258014-lzzi4rnz 105 33 . . . cord-258014-lzzi4rnz 106 1 It -PRON- PRP cord-258014-lzzi4rnz 106 2 starts start VBZ cord-258014-lzzi4rnz 106 3 with with IN cord-258014-lzzi4rnz 106 4 a a DT cord-258014-lzzi4rnz 106 5 PCR PCR NNP cord-258014-lzzi4rnz 106 6 step step NN cord-258014-lzzi4rnz 106 7 , , , cord-258014-lzzi4rnz 106 8 followed follow VBN cord-258014-lzzi4rnz 106 9 by by IN cord-258014-lzzi4rnz 106 10 a a DT cord-258014-lzzi4rnz 106 11 reverse reverse JJ cord-258014-lzzi4rnz 106 12 hybridization hybridization NN cord-258014-lzzi4rnz 106 13 protocol protocol NN cord-258014-lzzi4rnz 106 14 . . . cord-258014-lzzi4rnz 107 1 Thaw thaw VB cord-258014-lzzi4rnz 107 2 the the DT cord-258014-lzzi4rnz 107 3 DNA dna NN cord-258014-lzzi4rnz 107 4 previously previously RB cord-258014-lzzi4rnz 107 5 extracted extract VBN cord-258014-lzzi4rnz 107 6 from from IN cord-258014-lzzi4rnz 107 7 clinical clinical JJ cord-258014-lzzi4rnz 107 8 samples sample NNS cord-258014-lzzi4rnz 107 9 , , , cord-258014-lzzi4rnz 107 10 mix mix VB cord-258014-lzzi4rnz 107 11 by by IN cord-258014-lzzi4rnz 107 12 vortexing vortexe VBG cord-258014-lzzi4rnz 107 13 and and CC cord-258014-lzzi4rnz 107 14 spin spin VB cord-258014-lzzi4rnz 107 15 down down RP cord-258014-lzzi4rnz 107 16 the the DT cord-258014-lzzi4rnz 107 17 tubes tube NNS cord-258014-lzzi4rnz 107 18 to to TO cord-258014-lzzi4rnz 107 19 collect collect VB cord-258014-lzzi4rnz 107 20 all all DT cord-258014-lzzi4rnz 107 21 liquid liquid NN cord-258014-lzzi4rnz 107 22 at at IN cord-258014-lzzi4rnz 107 23 the the DT cord-258014-lzzi4rnz 107 24 bottom bottom NN cord-258014-lzzi4rnz 107 25 of of IN cord-258014-lzzi4rnz 107 26 the the DT cord-258014-lzzi4rnz 107 27 tube tube NN cord-258014-lzzi4rnz 107 28 . . . cord-258014-lzzi4rnz 108 1 The the DT cord-258014-lzzi4rnz 108 2 assay assay NN cord-258014-lzzi4rnz 108 3 uses use VBZ cord-258014-lzzi4rnz 108 4 SPF10 SPF10 JJR cord-258014-lzzi4rnz 108 5 primers primer NNS cord-258014-lzzi4rnz 108 6 to to TO cord-258014-lzzi4rnz 108 7 amplify amplify VB cord-258014-lzzi4rnz 108 8 a a DT cord-258014-lzzi4rnz 108 9 65-bp 65-bp CD cord-258014-lzzi4rnz 108 10 fragment fragment NN cord-258014-lzzi4rnz 108 11 of of IN cord-258014-lzzi4rnz 108 12 the the DT cord-258014-lzzi4rnz 108 13 L1 L1 NNP cord-258014-lzzi4rnz 108 14 open open JJ cord-258014-lzzi4rnz 108 15 reading reading NN cord-258014-lzzi4rnz 108 16 frame frame NN cord-258014-lzzi4rnz 108 17 of of IN cord-258014-lzzi4rnz 108 18 HPV hpv NN cord-258014-lzzi4rnz 108 19 genotypes genotype NNS cord-258014-lzzi4rnz 108 20 , , , cord-258014-lzzi4rnz 108 21 followed follow VBN cord-258014-lzzi4rnz 108 22 by by IN cord-258014-lzzi4rnz 108 23 a a DT cord-258014-lzzi4rnz 108 24 reverse reverse JJ cord-258014-lzzi4rnz 108 25 - - HYPH cord-258014-lzzi4rnz 108 26 hybridization hybridization NN cord-258014-lzzi4rnz 108 27 step step NN cord-258014-lzzi4rnz 108 28 . . . cord-258014-lzzi4rnz 109 1 The the DT cord-258014-lzzi4rnz 109 2 65-bp 65-bp CD cord-258014-lzzi4rnz 109 3 PCR PCR NNP cord-258014-lzzi4rnz 109 4 fragment fragment NN cord-258014-lzzi4rnz 109 5 assay assay NN cord-258014-lzzi4rnz 109 6 amplifies amplify VBZ cord-258014-lzzi4rnz 109 7 the the DT cord-258014-lzzi4rnz 109 8 following follow VBG cord-258014-lzzi4rnz 109 9 common common JJ cord-258014-lzzi4rnz 109 10 mucosal mucosal JJ cord-258014-lzzi4rnz 109 11 HPV hpv NN cord-258014-lzzi4rnz 109 12 genotypes genotype NNS cord-258014-lzzi4rnz 109 13 : : : cord-258014-lzzi4rnz 109 14 Prepare prepare VB cord-258014-lzzi4rnz 109 15 the the DT cord-258014-lzzi4rnz 109 16 master master NN cord-258014-lzzi4rnz 109 17 mix mix NN cord-258014-lzzi4rnz 109 18 for for IN cord-258014-lzzi4rnz 109 19 the the DT cord-258014-lzzi4rnz 109 20 PCR PCR NNP cord-258014-lzzi4rnz 109 21 . . . cord-258014-lzzi4rnz 110 1 At at IN cord-258014-lzzi4rnz 110 2 first first JJ cord-258014-lzzi4rnz 110 3 use use NN cord-258014-lzzi4rnz 110 4 of of IN cord-258014-lzzi4rnz 110 5 the the DT cord-258014-lzzi4rnz 110 6 PCR PCR NNP cord-258014-lzzi4rnz 110 7 master master NN cord-258014-lzzi4rnz 110 8 mix mix NN cord-258014-lzzi4rnz 110 9 ( ( -LRB- cord-258014-lzzi4rnz 110 10 vial vial NN cord-258014-lzzi4rnz 110 11 A1 A1 NNP cord-258014-lzzi4rnz 110 12 ) ) -RRB- cord-258014-lzzi4rnz 110 13 , , , cord-258014-lzzi4rnz 110 14 it -PRON- PRP cord-258014-lzzi4rnz 110 15 is be VBZ cord-258014-lzzi4rnz 110 16 recommended recommend VBN cord-258014-lzzi4rnz 110 17 to to TO cord-258014-lzzi4rnz 110 18 mix mix VB cord-258014-lzzi4rnz 110 19 the the DT cord-258014-lzzi4rnz 110 20 contents content NNS cord-258014-lzzi4rnz 110 21 of of IN cord-258014-lzzi4rnz 110 22 the the DT cord-258014-lzzi4rnz 110 23 vial vial NN cord-258014-lzzi4rnz 110 24 on on IN cord-258014-lzzi4rnz 110 25 a a DT cord-258014-lzzi4rnz 110 26 Vortex vortex NN cord-258014-lzzi4rnz 110 27 mixer mixer NN cord-258014-lzzi4rnz 110 28 and and CC cord-258014-lzzi4rnz 110 29 aliquot aliquot VBD cord-258014-lzzi4rnz 110 30 the the DT cord-258014-lzzi4rnz 110 31 contents content NNS cord-258014-lzzi4rnz 110 32 in in IN cord-258014-lzzi4rnz 110 33 ready ready JJ cord-258014-lzzi4rnz 110 34 - - HYPH cord-258014-lzzi4rnz 110 35 to to IN cord-258014-lzzi4rnz 110 36 - - HYPH cord-258014-lzzi4rnz 110 37 use use NN cord-258014-lzzi4rnz 110 38 portions portion NNS cord-258014-lzzi4rnz 110 39 of of IN cord-258014-lzzi4rnz 110 40 40 40 CD cord-258014-lzzi4rnz 110 41 µL. µl. NN cord-258014-lzzi4rnz 111 1 This this DT cord-258014-lzzi4rnz 111 2 further further RB cord-258014-lzzi4rnz 111 3 reduces reduce VBZ cord-258014-lzzi4rnz 111 4 the the DT cord-258014-lzzi4rnz 111 5 risk risk NN cord-258014-lzzi4rnz 111 6 of of IN cord-258014-lzzi4rnz 111 7 contamination contamination NN cord-258014-lzzi4rnz 111 8 . . . cord-258014-lzzi4rnz 112 1 After after IN cord-258014-lzzi4rnz 112 2 aliquoting aliquoting NN cord-258014-lzzi4rnz 112 3 , , , cord-258014-lzzi4rnz 112 4 store store NN cord-258014-lzzi4rnz 112 5 at at IN cord-258014-lzzi4rnz 112 6 −20 −20 NNP cord-258014-lzzi4rnz 112 7 • • NNP cord-258014-lzzi4rnz 112 8 C C NNP cord-258014-lzzi4rnz 112 9 4 4 CD cord-258014-lzzi4rnz 112 10 . . . cord-258014-lzzi4rnz 113 1 Prepare prepare VB cord-258014-lzzi4rnz 113 2 the the DT cord-258014-lzzi4rnz 113 3 tubes/96-well tubes/96-well NNP cord-258014-lzzi4rnz 113 4 plates plate NNS cord-258014-lzzi4rnz 113 5 ( ( -LRB- cord-258014-lzzi4rnz 113 6 depending depend VBG cord-258014-lzzi4rnz 113 7 on on IN cord-258014-lzzi4rnz 113 8 the the DT cord-258014-lzzi4rnz 113 9 number number NN cord-258014-lzzi4rnz 113 10 of of IN cord-258014-lzzi4rnz 113 11 samples sample NNS cord-258014-lzzi4rnz 113 12 ) ) -RRB- cord-258014-lzzi4rnz 113 13 considering consider VBG cord-258014-lzzi4rnz 113 14 the the DT cord-258014-lzzi4rnz 113 15 number number NN cord-258014-lzzi4rnz 113 16 of of IN cord-258014-lzzi4rnz 113 17 the the DT cord-258014-lzzi4rnz 113 18 samples sample NNS cord-258014-lzzi4rnz 113 19 to to TO cord-258014-lzzi4rnz 113 20 process process VB cord-258014-lzzi4rnz 113 21 and and CC cord-258014-lzzi4rnz 113 22 include include VBP cord-258014-lzzi4rnz 113 23 negative negative JJ cord-258014-lzzi4rnz 113 24 and and CC cord-258014-lzzi4rnz 113 25 positive positive JJ cord-258014-lzzi4rnz 113 26 controls control NNS cord-258014-lzzi4rnz 113 27 provided provide VBN cord-258014-lzzi4rnz 113 28 in in IN cord-258014-lzzi4rnz 113 29 the the DT cord-258014-lzzi4rnz 113 30 kit kit NN cord-258014-lzzi4rnz 113 31 . . . cord-258014-lzzi4rnz 114 1 Mark mark VB cord-258014-lzzi4rnz 114 2 each each DT cord-258014-lzzi4rnz 114 3 PCR PCR NNP cord-258014-lzzi4rnz 114 4 reaction reaction NN cord-258014-lzzi4rnz 114 5 vial vial NN cord-258014-lzzi4rnz 114 6 / / SYM cord-258014-lzzi4rnz 114 7 PCR pcr NN cord-258014-lzzi4rnz 114 8 plate plate NN cord-258014-lzzi4rnz 114 9 . . . cord-258014-lzzi4rnz 115 1 6 6 LS cord-258014-lzzi4rnz 115 2 . . . cord-258014-lzzi4rnz 116 1 Spin spin VB cord-258014-lzzi4rnz 116 2 down down RP cord-258014-lzzi4rnz 116 3 the the DT cord-258014-lzzi4rnz 116 4 liquid liquid NN cord-258014-lzzi4rnz 116 5 in in IN cord-258014-lzzi4rnz 116 6 the the DT cord-258014-lzzi4rnz 116 7 tubes tube NNS cord-258014-lzzi4rnz 116 8 with with IN cord-258014-lzzi4rnz 116 9 the the DT cord-258014-lzzi4rnz 116 10 processed process VBN cord-258014-lzzi4rnz 116 11 specimen specimen NN cord-258014-lzzi4rnz 116 12 for for IN cord-258014-lzzi4rnz 116 13 15 15 CD cord-258014-lzzi4rnz 116 14 s s NNS cord-258014-lzzi4rnz 116 15 at at IN cord-258014-lzzi4rnz 116 16 14,000 14,000 CD cord-258014-lzzi4rnz 116 17 rpm rpm NN cord-258014-lzzi4rnz 116 18 in in IN cord-258014-lzzi4rnz 116 19 a a DT cord-258014-lzzi4rnz 116 20 minicentrifuge minicentrifuge NN cord-258014-lzzi4rnz 116 21 . . . cord-258014-lzzi4rnz 117 1 7 7 CD cord-258014-lzzi4rnz 117 2 . . . cord-258014-lzzi4rnz 118 1 Add add VB cord-258014-lzzi4rnz 118 2 ( ( -LRB- cord-258014-lzzi4rnz 118 3 one one CD cord-258014-lzzi4rnz 118 4 by by IN cord-258014-lzzi4rnz 118 5 one one CD cord-258014-lzzi4rnz 118 6 ) ) -RRB- cord-258014-lzzi4rnz 119 1 10 10 CD cord-258014-lzzi4rnz 119 2 µL µl CD cord-258014-lzzi4rnz 119 3 of of IN cord-258014-lzzi4rnz 119 4 the the DT cord-258014-lzzi4rnz 119 5 supernatant supernatant NN cord-258014-lzzi4rnz 119 6 of of IN cord-258014-lzzi4rnz 119 7 the the DT cord-258014-lzzi4rnz 119 8 processed process VBN cord-258014-lzzi4rnz 119 9 sample sample NN cord-258014-lzzi4rnz 119 10 to to IN cord-258014-lzzi4rnz 119 11 the the DT cord-258014-lzzi4rnz 119 12 appropriate appropriate JJ cord-258014-lzzi4rnz 119 13 vials vial NNS cord-258014-lzzi4rnz 119 14 with with IN cord-258014-lzzi4rnz 119 15 PCR PCR NNP cord-258014-lzzi4rnz 119 16 master master NN cord-258014-lzzi4rnz 119 17 mix mix NN cord-258014-lzzi4rnz 119 18 . . . cord-258014-lzzi4rnz 120 1 The the DT cord-258014-lzzi4rnz 120 2 vials vial NNS cord-258014-lzzi4rnz 120 3 intended intend VBN cord-258014-lzzi4rnz 120 4 for for IN cord-258014-lzzi4rnz 120 5 the the DT cord-258014-lzzi4rnz 120 6 HPV HPV NNP cord-258014-lzzi4rnz 120 7 negative negative JJ cord-258014-lzzi4rnz 120 8 and and CC cord-258014-lzzi4rnz 120 9 positive positive JJ cord-258014-lzzi4rnz 120 10 PCR PCR NNP cord-258014-lzzi4rnz 120 11 controls control NNS cord-258014-lzzi4rnz 120 12 remain remain VBP cord-258014-lzzi4rnz 120 13 closed closed JJ cord-258014-lzzi4rnz 120 14 . . . cord-258014-lzzi4rnz 121 1 Cap cap VB cord-258014-lzzi4rnz 121 2 each each DT cord-258014-lzzi4rnz 121 3 vial vial NN cord-258014-lzzi4rnz 121 4 after after IN cord-258014-lzzi4rnz 121 5 the the DT cord-258014-lzzi4rnz 121 6 addition addition NN cord-258014-lzzi4rnz 121 7 of of IN cord-258014-lzzi4rnz 121 8 DNA dna NN cord-258014-lzzi4rnz 121 9 before before IN cord-258014-lzzi4rnz 121 10 proceeding proceed VBG cord-258014-lzzi4rnz 121 11 with with IN cord-258014-lzzi4rnz 121 12 the the DT cord-258014-lzzi4rnz 121 13 next next JJ cord-258014-lzzi4rnz 121 14 . . . cord-258014-lzzi4rnz 122 1 8 8 CD cord-258014-lzzi4rnz 122 2 . . . cord-258014-lzzi4rnz 123 1 Mix Mix NNP cord-258014-lzzi4rnz 123 2 HPV HPV NNP cord-258014-lzzi4rnz 123 3 positive positive JJ cord-258014-lzzi4rnz 123 4 PCR PCR NNP cord-258014-lzzi4rnz 123 5 control control NN cord-258014-lzzi4rnz 123 6 ( ( -LRB- cord-258014-lzzi4rnz 123 7 vial vial NN cord-258014-lzzi4rnz 123 8 B1 b1 NN cord-258014-lzzi4rnz 123 9 ) ) -RRB- cord-258014-lzzi4rnz 123 10 on on IN cord-258014-lzzi4rnz 123 11 a a DT cord-258014-lzzi4rnz 123 12 Vortex vortex NN cord-258014-lzzi4rnz 123 13 mixer mixer NN cord-258014-lzzi4rnz 123 14 , , , cord-258014-lzzi4rnz 123 15 add add VB cord-258014-lzzi4rnz 123 16 10 10 CD cord-258014-lzzi4rnz 123 17 µL µl CD cord-258014-lzzi4rnz 123 18 to to IN cord-258014-lzzi4rnz 123 19 the the DT cord-258014-lzzi4rnz 123 20 appropriate appropriate JJ cord-258014-lzzi4rnz 123 21 vial vial NN cord-258014-lzzi4rnz 123 22 , , , cord-258014-lzzi4rnz 123 23 and and CC cord-258014-lzzi4rnz 123 24 close close VB cord-258014-lzzi4rnz 123 25 the the DT cord-258014-lzzi4rnz 123 26 vial vial NN cord-258014-lzzi4rnz 123 27 . . . cord-258014-lzzi4rnz 124 1 9 9 LS cord-258014-lzzi4rnz 124 2 . . . cord-258014-lzzi4rnz 125 1 Use use VB cord-258014-lzzi4rnz 125 2 clean clean JJ cord-258014-lzzi4rnz 125 3 filter filter NN cord-258014-lzzi4rnz 125 4 tips tip NNS cord-258014-lzzi4rnz 125 5 for for IN cord-258014-lzzi4rnz 125 6 each each DT cord-258014-lzzi4rnz 125 7 sample sample NN cord-258014-lzzi4rnz 125 8 and and CC cord-258014-lzzi4rnz 125 9 mix mix VB cord-258014-lzzi4rnz 125 10 each each DT cord-258014-lzzi4rnz 125 11 sample sample NN cord-258014-lzzi4rnz 125 12 with with IN cord-258014-lzzi4rnz 125 13 the the DT cord-258014-lzzi4rnz 125 14 PCR PCR NNP cord-258014-lzzi4rnz 125 15 mix mix NN cord-258014-lzzi4rnz 125 16 by by IN cord-258014-lzzi4rnz 125 17 pipetting pipette VBG cord-258014-lzzi4rnz 125 18 up up RP cord-258014-lzzi4rnz 125 19 and and CC cord-258014-lzzi4rnz 125 20 down down IN cord-258014-lzzi4rnz 125 21 a a DT cord-258014-lzzi4rnz 125 22 few few JJ cord-258014-lzzi4rnz 125 23 times time NNS cord-258014-lzzi4rnz 125 24 . . . cord-258014-lzzi4rnz 126 1 In in IN cord-258014-lzzi4rnz 126 2 the the DT cord-258014-lzzi4rnz 126 3 second second JJ cord-258014-lzzi4rnz 126 4 step step NN cord-258014-lzzi4rnz 126 5 , , , cord-258014-lzzi4rnz 126 6 the the DT cord-258014-lzzi4rnz 126 7 amplified amplify VBN cord-258014-lzzi4rnz 126 8 fragments fragment NNS cord-258014-lzzi4rnz 126 9 undergo undergo VBP cord-258014-lzzi4rnz 126 10 a a DT cord-258014-lzzi4rnz 126 11 line line NN cord-258014-lzzi4rnz 126 12 probe probe NN cord-258014-lzzi4rnz 126 13 assay assay NN cord-258014-lzzi4rnz 126 14 by by IN cord-258014-lzzi4rnz 126 15 reverse reverse JJ cord-258014-lzzi4rnz 126 16 - - HYPH cord-258014-lzzi4rnz 126 17 hybridization hybridization NN cord-258014-lzzi4rnz 126 18 assay assay NN cord-258014-lzzi4rnz 126 19 performed perform VBN cord-258014-lzzi4rnz 126 20 by by IN cord-258014-lzzi4rnz 126 21 using use VBG cord-258014-lzzi4rnz 126 22 the the DT cord-258014-lzzi4rnz 126 23 portion portion NN cord-258014-lzzi4rnz 126 24 of of IN cord-258014-lzzi4rnz 126 25 the the DT cord-258014-lzzi4rnz 126 26 kit kit NN cord-258014-lzzi4rnz 126 27 named name VBN cord-258014-lzzi4rnz 126 28 RHA RHA NNP cord-258014-lzzi4rnz 126 29 kit kit NN cord-258014-lzzi4rnz 126 30 HPV HPV NNP cord-258014-lzzi4rnz 126 31 SPF10-LiPA25 spf10-lipa25 JJ cord-258014-lzzi4rnz 126 32 . . . cord-258014-lzzi4rnz 127 1 In in IN cord-258014-lzzi4rnz 127 2 this this DT cord-258014-lzzi4rnz 127 3 second second JJ cord-258014-lzzi4rnz 127 4 step step NN cord-258014-lzzi4rnz 127 5 , , , cord-258014-lzzi4rnz 127 6 the the DT cord-258014-lzzi4rnz 127 7 biotinylated biotinylated JJ cord-258014-lzzi4rnz 127 8 amplicons amplicon NNS cord-258014-lzzi4rnz 127 9 are be VBP cord-258014-lzzi4rnz 127 10 denatured denature VBN cord-258014-lzzi4rnz 127 11 and and CC cord-258014-lzzi4rnz 127 12 then then RB cord-258014-lzzi4rnz 127 13 hybridized hybridize VBN cord-258014-lzzi4rnz 127 14 with with IN cord-258014-lzzi4rnz 127 15 specific specific JJ cord-258014-lzzi4rnz 127 16 oligonucleotide oligonucleotide JJ cord-258014-lzzi4rnz 127 17 probes probe NNS cord-258014-lzzi4rnz 127 18 immobilized immobilize VBN cord-258014-lzzi4rnz 127 19 as as IN cord-258014-lzzi4rnz 127 20 parallel parallel JJ cord-258014-lzzi4rnz 127 21 lines line NNS cord-258014-lzzi4rnz 127 22 in in IN cord-258014-lzzi4rnz 127 23 membrane membrane NN cord-258014-lzzi4rnz 127 24 strips strip NNS cord-258014-lzzi4rnz 127 25 . . . cord-258014-lzzi4rnz 128 1 After after IN cord-258014-lzzi4rnz 128 2 immobilization immobilization NN cord-258014-lzzi4rnz 128 3 of of IN cord-258014-lzzi4rnz 128 4 the the DT cord-258014-lzzi4rnz 128 5 oligonucleotides oligonucleotide NNS cord-258014-lzzi4rnz 128 6 on on IN cord-258014-lzzi4rnz 128 7 the the DT cord-258014-lzzi4rnz 128 8 strips strip NNS cord-258014-lzzi4rnz 128 9 , , , cord-258014-lzzi4rnz 128 10 these these DT cord-258014-lzzi4rnz 128 11 were be VBD cord-258014-lzzi4rnz 128 12 washed wash VBN cord-258014-lzzi4rnz 128 13 , , , cord-258014-lzzi4rnz 128 14 and and CC cord-258014-lzzi4rnz 128 15 streptavidin streptavidin JJ cord-258014-lzzi4rnz 128 16 alkaline alkaline JJ cord-258014-lzzi4rnz 128 17 phosphatase phosphatase NN cord-258014-lzzi4rnz 128 18 ( ( -LRB- cord-258014-lzzi4rnz 128 19 SAP SAP NNP cord-258014-lzzi4rnz 128 20 ) ) -RRB- cord-258014-lzzi4rnz 128 21 is be VBZ cord-258014-lzzi4rnz 128 22 added add VBN cord-258014-lzzi4rnz 128 23 to to TO cord-258014-lzzi4rnz 128 24 bind bind VB cord-258014-lzzi4rnz 128 25 to to IN cord-258014-lzzi4rnz 128 26 the the DT cord-258014-lzzi4rnz 128 27 biotinylated biotinylated JJ cord-258014-lzzi4rnz 128 28 hybrid hybrid NN cord-258014-lzzi4rnz 128 29 formed form VBN cord-258014-lzzi4rnz 128 30 , , , cord-258014-lzzi4rnz 128 31 yielding yield VBG cord-258014-lzzi4rnz 128 32 a a DT cord-258014-lzzi4rnz 128 33 purple purple JJ cord-258014-lzzi4rnz 128 34 precipitate precipitate NN cord-258014-lzzi4rnz 128 35 in in IN cord-258014-lzzi4rnz 128 36 the the DT cord-258014-lzzi4rnz 128 37 strip strip NN cord-258014-lzzi4rnz 128 38 that that WDT cord-258014-lzzi4rnz 128 39 visually visually RB cord-258014-lzzi4rnz 128 40 determines determine VBZ cord-258014-lzzi4rnz 128 41 the the DT cord-258014-lzzi4rnz 128 42 specific specific JJ cord-258014-lzzi4rnz 128 43 HPV hpv NN cord-258014-lzzi4rnz 128 44 type type NN cord-258014-lzzi4rnz 128 45 compared compare VBN cord-258014-lzzi4rnz 128 46 to to IN cord-258014-lzzi4rnz 128 47 the the DT cord-258014-lzzi4rnz 128 48 kit kit NN cord-258014-lzzi4rnz 128 49 - - HYPH cord-258014-lzzi4rnz 128 50 provided provide VBN cord-258014-lzzi4rnz 128 51 controls control NNS cord-258014-lzzi4rnz 128 52 . . . cord-258014-lzzi4rnz 129 1 The the DT cord-258014-lzzi4rnz 129 2 LiPA LiPA NNP cord-258014-lzzi4rnz 129 3 strips strip NNS cord-258014-lzzi4rnz 129 4 are be VBP cord-258014-lzzi4rnz 129 5 visually visually RB cord-258014-lzzi4rnz 129 6 inspected inspect VBN cord-258014-lzzi4rnz 129 7 and and CC cord-258014-lzzi4rnz 129 8 interpreted interpret VBD cord-258014-lzzi4rnz 129 9 following follow VBG cord-258014-lzzi4rnz 129 10 the the DT cord-258014-lzzi4rnz 129 11 standardized standardized JJ cord-258014-lzzi4rnz 129 12 reference reference NN cord-258014-lzzi4rnz 129 13 guide guide NN cord-258014-lzzi4rnz 129 14 provided provide VBN cord-258014-lzzi4rnz 129 15 by by IN cord-258014-lzzi4rnz 129 16 the the DT cord-258014-lzzi4rnz 129 17 kit kit NN cord-258014-lzzi4rnz 129 18 's 's POS cord-258014-lzzi4rnz 129 19 manual manual NN cord-258014-lzzi4rnz 129 20 . . . cord-258014-lzzi4rnz 130 1 12 12 CD cord-258014-lzzi4rnz 130 2 . . . cord-258014-lzzi4rnz 131 1 Prewarm Prewarm NNP cord-258014-lzzi4rnz 131 2 the the DT cord-258014-lzzi4rnz 131 3 vials vial NNS cord-258014-lzzi4rnz 131 4 with with IN cord-258014-lzzi4rnz 131 5 the the DT cord-258014-lzzi4rnz 131 6 hybridization hybridization NN cord-258014-lzzi4rnz 131 7 Solution solution NN cord-258014-lzzi4rnz 131 8 ( ( -LRB- cord-258014-lzzi4rnz 131 9 HS HS NNP cord-258014-lzzi4rnz 131 10 ) ) -RRB- cord-258014-lzzi4rnz 131 11 and and CC cord-258014-lzzi4rnz 131 12 the the DT cord-258014-lzzi4rnz 131 13 Stringent Stringent NNP cord-258014-lzzi4rnz 131 14 Wash Wash NNP cord-258014-lzzi4rnz 131 15 Solution Solution NNP cord-258014-lzzi4rnz 131 16 ( ( -LRB- cord-258014-lzzi4rnz 131 17 SW SW NNP cord-258014-lzzi4rnz 131 18 ) ) -RRB- cord-258014-lzzi4rnz 132 1 to to IN cord-258014-lzzi4rnz 132 2 at at RB cord-258014-lzzi4rnz 132 3 least least JJS cord-258014-lzzi4rnz 132 4 37 37 CD cord-258014-lzzi4rnz 132 5 • • NN cord-258014-lzzi4rnz 132 6 C c NN cord-258014-lzzi4rnz 133 1 but but CC cord-258014-lzzi4rnz 133 2 must must MD cord-258014-lzzi4rnz 133 3 not not RB cord-258014-lzzi4rnz 133 4 exceed exceed VB cord-258014-lzzi4rnz 133 5 the the DT cord-258014-lzzi4rnz 133 6 hybridization hybridization NN cord-258014-lzzi4rnz 133 7 temperature temperature NN cord-258014-lzzi4rnz 133 8 of of IN cord-258014-lzzi4rnz 133 9 49 49 CD cord-258014-lzzi4rnz 133 10 • • NN cord-258014-lzzi4rnz 133 11 C c NN cord-258014-lzzi4rnz 134 1 ( ( -LRB- cord-258014-lzzi4rnz 134 2 all all DT cord-258014-lzzi4rnz 134 3 crystals crystal NNS cord-258014-lzzi4rnz 134 4 should should MD cord-258014-lzzi4rnz 134 5 be be VB cord-258014-lzzi4rnz 134 6 dissolved dissolve VBN cord-258014-lzzi4rnz 134 7 before before IN cord-258014-lzzi4rnz 134 8 the the DT cord-258014-lzzi4rnz 134 9 opening opening NN cord-258014-lzzi4rnz 134 10 of of IN cord-258014-lzzi4rnz 134 11 the the DT cord-258014-lzzi4rnz 134 12 vial vial NN cord-258014-lzzi4rnz 134 13 ) ) -RRB- cord-258014-lzzi4rnz 134 14 . . . cord-258014-lzzi4rnz 135 1 Currently currently RB cord-258014-lzzi4rnz 135 2 , , , cord-258014-lzzi4rnz 135 3 it -PRON- PRP cord-258014-lzzi4rnz 135 4 is be VBZ cord-258014-lzzi4rnz 135 5 cheaper cheap JJR cord-258014-lzzi4rnz 135 6 to to TO cord-258014-lzzi4rnz 135 7 extract extract VB cord-258014-lzzi4rnz 135 8 good good JJ cord-258014-lzzi4rnz 135 9 quality quality NN cord-258014-lzzi4rnz 135 10 DNA dna NN cord-258014-lzzi4rnz 135 11 and and CC cord-258014-lzzi4rnz 135 12 send send VB cord-258014-lzzi4rnz 135 13 directly directly RB cord-258014-lzzi4rnz 135 14 to to IN cord-258014-lzzi4rnz 135 15 any any DT cord-258014-lzzi4rnz 135 16 company company NN cord-258014-lzzi4rnz 135 17 for for IN cord-258014-lzzi4rnz 135 18 outsourced outsource VBN cord-258014-lzzi4rnz 135 19 sequencing sequencing NN cord-258014-lzzi4rnz 135 20 of of IN cord-258014-lzzi4rnz 135 21 16S 16S NNP cord-258014-lzzi4rnz 135 22 rDNA rDNA NNP cord-258014-lzzi4rnz 135 23 genes gene NNS cord-258014-lzzi4rnz 135 24 . . . cord-258014-lzzi4rnz 136 1 To to TO cord-258014-lzzi4rnz 136 2 characterize characterize VB cord-258014-lzzi4rnz 136 3 the the DT cord-258014-lzzi4rnz 136 4 microbiota microbiota NN cord-258014-lzzi4rnz 136 5 , , , cord-258014-lzzi4rnz 136 6 one one CD cord-258014-lzzi4rnz 136 7 sequences sequence NNS cord-258014-lzzi4rnz 136 8 the the DT cord-258014-lzzi4rnz 136 9 V4 V4 NNP cord-258014-lzzi4rnz 136 10 hypervariable hypervariable JJ cord-258014-lzzi4rnz 136 11 region region NN cord-258014-lzzi4rnz 136 12 of of IN cord-258014-lzzi4rnz 136 13 the the DT cord-258014-lzzi4rnz 136 14 16S 16S NNP cord-258014-lzzi4rnz 136 15 ribosomal ribosomal NNP cord-258014-lzzi4rnz 136 16 RNA RNA NNP cord-258014-lzzi4rnz 136 17 using use VBG cord-258014-lzzi4rnz 136 18 the the DT cord-258014-lzzi4rnz 136 19 universal universal JJ cord-258014-lzzi4rnz 136 20 bacterial bacterial JJ cord-258014-lzzi4rnz 136 21 primers primer NNS cord-258014-lzzi4rnz 136 22 : : : cord-258014-lzzi4rnz 136 23 515F 515F . cord-258014-lzzi4rnz 137 1 ( ( -LRB- cord-258014-lzzi4rnz 137 2 5 5 CD cord-258014-lzzi4rnz 137 3 GTGCCAGCMGCCGCGGTAA GTGCCAGCMGCCGCGGTAA NNP cord-258014-lzzi4rnz 137 4 3 3 CD cord-258014-lzzi4rnz 137 5 ) ) -RRB- cord-258014-lzzi4rnz 137 6 and and CC cord-258014-lzzi4rnz 138 1 806R 806R NNP cord-258014-lzzi4rnz 138 2 ( ( -LRB- cord-258014-lzzi4rnz 138 3 5 5 CD cord-258014-lzzi4rnz 138 4 GGACTACHVGGGTWTCTAAT GGACTACHVGGGTWTCTAAT NNP cord-258014-lzzi4rnz 138 5 3 3 CD cord-258014-lzzi4rnz 138 6 ) ) -RRB- cord-258014-lzzi4rnz 139 1 as as IN cord-258014-lzzi4rnz 139 2 described describe VBN cord-258014-lzzi4rnz 139 3 in in IN cord-258014-lzzi4rnz 139 4 the the DT cord-258014-lzzi4rnz 139 5 Earth Earth NNP cord-258014-lzzi4rnz 139 6 Microbiome Microbiome NNP cord-258014-lzzi4rnz 139 7 Project Project NNP cord-258014-lzzi4rnz 139 8 ( ( -LRB- cord-258014-lzzi4rnz 139 9 EMP EMP NNP cord-258014-lzzi4rnz 139 10 ; ; : cord-258014-lzzi4rnz 139 11 http://www.earthmicrobiome.org/emp-standard-protocols/16s/ http://www.earthmicrobiome.org/emp-standard-protocols/16s/ RB cord-258014-lzzi4rnz 139 12 ; ; : cord-258014-lzzi4rnz 139 13 ) ) -RRB- cord-258014-lzzi4rnz 140 1 [ [ -LRB- cord-258014-lzzi4rnz 140 2 27 27 CD cord-258014-lzzi4rnz 140 3 ] ] -RRB- cord-258014-lzzi4rnz 140 4 . . . cord-258014-lzzi4rnz 141 1 Once once IN cord-258014-lzzi4rnz 141 2 the the DT cord-258014-lzzi4rnz 141 3 facility facility NN cord-258014-lzzi4rnz 141 4 sends send VBZ cord-258014-lzzi4rnz 141 5 the the DT cord-258014-lzzi4rnz 141 6 data datum NNS cord-258014-lzzi4rnz 141 7 , , , cord-258014-lzzi4rnz 141 8 raw raw JJ cord-258014-lzzi4rnz 141 9 FASTQ FASTQ NNP cord-258014-lzzi4rnz 141 10 reads read VBZ cord-258014-lzzi4rnz 141 11 can can MD cord-258014-lzzi4rnz 141 12 be be VB cord-258014-lzzi4rnz 141 13 uploaded upload VBN cord-258014-lzzi4rnz 141 14 to to IN cord-258014-lzzi4rnz 141 15 QIITA QIITA NNP cord-258014-lzzi4rnz 141 16 https://qiita.ucsd.edu/ https://qiita.ucsd.edu/ ADD cord-258014-lzzi4rnz 141 17 , , , cord-258014-lzzi4rnz 141 18 an an DT cord-258014-lzzi4rnz 141 19 entirely entirely RB cord-258014-lzzi4rnz 141 20 open open JJ cord-258014-lzzi4rnz 141 21 - - HYPH cord-258014-lzzi4rnz 141 22 source source NN cord-258014-lzzi4rnz 141 23 microbial microbial JJ cord-258014-lzzi4rnz 141 24 study study NN cord-258014-lzzi4rnz 141 25 management management NN cord-258014-lzzi4rnz 141 26 platform platform NN cord-258014-lzzi4rnz 141 27 [ [ -LRB- cord-258014-lzzi4rnz 141 28 28 28 CD cord-258014-lzzi4rnz 141 29 ] ] -RRB- cord-258014-lzzi4rnz 141 30 . . . cord-258014-lzzi4rnz 142 1 It -PRON- PRP cord-258014-lzzi4rnz 142 2 allows allow VBZ cord-258014-lzzi4rnz 142 3 users user NNS cord-258014-lzzi4rnz 142 4 to to TO cord-258014-lzzi4rnz 142 5 keep keep VB cord-258014-lzzi4rnz 142 6 track track NN cord-258014-lzzi4rnz 142 7 of of IN cord-258014-lzzi4rnz 142 8 multiple multiple JJ cord-258014-lzzi4rnz 142 9 studies study NNS cord-258014-lzzi4rnz 142 10 with with IN cord-258014-lzzi4rnz 142 11 multiple multiple JJ cord-258014-lzzi4rnz 142 12 omics omic NNS cord-258014-lzzi4rnz 142 13 data datum NNS cord-258014-lzzi4rnz 142 14 , , , cord-258014-lzzi4rnz 142 15 and and CC cord-258014-lzzi4rnz 142 16 use use VB cord-258014-lzzi4rnz 142 17 stringent stringent JJ cord-258014-lzzi4rnz 142 18 quality quality NN cord-258014-lzzi4rnz 142 19 criteria criterion NNS cord-258014-lzzi4rnz 142 20 PHRED PHRED NNP cord-258014-lzzi4rnz 142 21 scores score NNS cord-258014-lzzi4rnz 142 22 ( ( -LRB- cord-258014-lzzi4rnz 142 23 quality quality NN cord-258014-lzzi4rnz 142 24 score score NN cord-258014-lzzi4rnz 142 25 of of IN cord-258014-lzzi4rnz 142 26 the the DT cord-258014-lzzi4rnz 142 27 nucleotides nucleotide NNS cord-258014-lzzi4rnz 142 28 generated generate VBN cord-258014-lzzi4rnz 142 29 by by IN cord-258014-lzzi4rnz 142 30 automated automate VBN cord-258014-lzzi4rnz 142 31 DNA dna NN cord-258014-lzzi4rnz 142 32 sequencing sequencing NN cord-258014-lzzi4rnz 142 33 ) ) -RRB- cord-258014-lzzi4rnz 142 34 with with IN cord-258014-lzzi4rnz 142 35 an an DT cord-258014-lzzi4rnz 142 36 ASCII ascii JJ cord-258014-lzzi4rnz 142 37 offset offset NN cord-258014-lzzi4rnz 142 38 of of IN cord-258014-lzzi4rnz 142 39 33 33 CD cord-258014-lzzi4rnz 142 40 , , , cord-258014-lzzi4rnz 142 41 and and CC cord-258014-lzzi4rnz 142 42 the the DT cord-258014-lzzi4rnz 142 43 user user NN cord-258014-lzzi4rnz 142 44 can can MD cord-258014-lzzi4rnz 142 45 choose choose VB cord-258014-lzzi4rnz 142 46 the the DT cord-258014-lzzi4rnz 142 47 length length NN cord-258014-lzzi4rnz 142 48 of of IN cord-258014-lzzi4rnz 142 49 the the DT cord-258014-lzzi4rnz 142 50 reads read NNS cord-258014-lzzi4rnz 142 51 for for IN cord-258014-lzzi4rnz 142 52 trimming trim VBG cord-258014-lzzi4rnz 142 53 , , , cord-258014-lzzi4rnz 142 54 demultiplexing demultiplexing NN cord-258014-lzzi4rnz 142 55 and and CC cord-258014-lzzi4rnz 142 56 binning binning NN cord-258014-lzzi4rnz 142 57 using use VBG cord-258014-lzzi4rnz 142 58 a a DT cord-258014-lzzi4rnz 142 59 given give VBN cord-258014-lzzi4rnz 142 60 database database NN cord-258014-lzzi4rnz 142 61 ( ( -LRB- cord-258014-lzzi4rnz 142 62 Greengenes Greengenes NNP cord-258014-lzzi4rnz 142 63 , , , cord-258014-lzzi4rnz 142 64 SILVA SILVA NNP cord-258014-lzzi4rnz 142 65 , , , cord-258014-lzzi4rnz 142 66 or or CC cord-258014-lzzi4rnz 142 67 the the DT cord-258014-lzzi4rnz 142 68 Ribosomal Ribosomal NNP cord-258014-lzzi4rnz 142 69 Database Database NNP cord-258014-lzzi4rnz 142 70 Project Project NNP cord-258014-lzzi4rnz 142 71 - - HYPH cord-258014-lzzi4rnz 142 72 RDP RDP NNP cord-258014-lzzi4rnz 142 73 ) ) -RRB- cord-258014-lzzi4rnz 142 74 . . . cord-258014-lzzi4rnz 143 1 The the DT cord-258014-lzzi4rnz 143 2 usefulness usefulness NN cord-258014-lzzi4rnz 143 3 of of IN cord-258014-lzzi4rnz 143 4 this this DT cord-258014-lzzi4rnz 143 5 platform platform NN cord-258014-lzzi4rnz 143 6 is be VBZ cord-258014-lzzi4rnz 143 7 that that IN cord-258014-lzzi4rnz 143 8 it -PRON- PRP cord-258014-lzzi4rnz 143 9 allows allow VBZ cord-258014-lzzi4rnz 143 10 data datum NNS cord-258014-lzzi4rnz 143 11 to to TO cord-258014-lzzi4rnz 143 12 be be VB cord-258014-lzzi4rnz 143 13 directly directly RB cord-258014-lzzi4rnz 143 14 made make VBN cord-258014-lzzi4rnz 143 15 public public JJ cord-258014-lzzi4rnz 143 16 via via IN cord-258014-lzzi4rnz 143 17 EBI EBI NNP cord-258014-lzzi4rnz 143 18 ( ( -LRB- cord-258014-lzzi4rnz 143 19 the the DT cord-258014-lzzi4rnz 143 20 European European NNP cord-258014-lzzi4rnz 143 21 Bioinformatics Bioinformatics NNP cord-258014-lzzi4rnz 143 22 Institute Institute NNP cord-258014-lzzi4rnz 143 23 ) ) -RRB- cord-258014-lzzi4rnz 143 24 , , , cord-258014-lzzi4rnz 143 25 a a DT cord-258014-lzzi4rnz 143 26 great great JJ cord-258014-lzzi4rnz 143 27 service service NN cord-258014-lzzi4rnz 143 28 offered offer VBN cord-258014-lzzi4rnz 143 29 by by IN cord-258014-lzzi4rnz 143 30 the the DT cord-258014-lzzi4rnz 143 31 QIITA QIITA NNP cord-258014-lzzi4rnz 143 32 team team NN cord-258014-lzzi4rnz 143 33 at at IN cord-258014-lzzi4rnz 143 34 the the DT cord-258014-lzzi4rnz 143 35 University University NNP cord-258014-lzzi4rnz 143 36 of of IN cord-258014-lzzi4rnz 143 37 California California NNP cord-258014-lzzi4rnz 143 38 San San NNP cord-258014-lzzi4rnz 143 39 Diego Diego NNP cord-258014-lzzi4rnz 143 40 ( ( -LRB- cord-258014-lzzi4rnz 143 41 UCSD UCSD NNP cord-258014-lzzi4rnz 143 42 ) ) -RRB- cord-258014-lzzi4rnz 143 43 . . . cord-258014-lzzi4rnz 144 1 After after IN cord-258014-lzzi4rnz 144 2 the the DT cord-258014-lzzi4rnz 144 3 OTU OTU NNP cord-258014-lzzi4rnz 144 4 table table NN cord-258014-lzzi4rnz 144 5 is be VBZ cord-258014-lzzi4rnz 144 6 prepared prepare VBN cord-258014-lzzi4rnz 144 7 in in IN cord-258014-lzzi4rnz 144 8 QIITA QIITA NNP cord-258014-lzzi4rnz 144 9 , , , cord-258014-lzzi4rnz 144 10 an an DT cord-258014-lzzi4rnz 144 11 alternative alternative NN cord-258014-lzzi4rnz 144 12 is be VBZ cord-258014-lzzi4rnz 144 13 to to TO cord-258014-lzzi4rnz 144 14 use use VB cord-258014-lzzi4rnz 144 15 QIIME2 QIIME2 NNP cord-258014-lzzi4rnz 144 16 for for IN cord-258014-lzzi4rnz 144 17 analyses analysis NNS cord-258014-lzzi4rnz 144 18 or or CC cord-258014-lzzi4rnz 144 19 R r NN cord-258014-lzzi4rnz 144 20 packages package NNS cord-258014-lzzi4rnz 144 21 such such JJ cord-258014-lzzi4rnz 144 22 as as IN cord-258014-lzzi4rnz 144 23 Bioconductor Bioconductor NNP cord-258014-lzzi4rnz 144 24 [ [ -LRB- cord-258014-lzzi4rnz 144 25 29 29 CD cord-258014-lzzi4rnz 144 26 ] ] -RRB- cord-258014-lzzi4rnz 144 27 or or CC cord-258014-lzzi4rnz 144 28 the the DT cord-258014-lzzi4rnz 144 29 quick quick JJ cord-258014-lzzi4rnz 144 30 tool tool NN cord-258014-lzzi4rnz 144 31 microbiomeanalyst microbiomeanalyst NN cord-258014-lzzi4rnz 144 32 ( ( -LRB- cord-258014-lzzi4rnz 144 33 https://www.microbiomeanalyst.ca https://www.microbiomeanalyst.ca VB cord-258014-lzzi4rnz 144 34 ) ) -RRB- cord-258014-lzzi4rnz 144 35 for for IN cord-258014-lzzi4rnz 144 36 preliminary preliminary JJ cord-258014-lzzi4rnz 144 37 analyses analysis NNS cord-258014-lzzi4rnz 144 38 [ [ -LRB- cord-258014-lzzi4rnz 144 39 30 30 CD cord-258014-lzzi4rnz 144 40 ] ] -RRB- cord-258014-lzzi4rnz 144 41 . . . cord-258014-lzzi4rnz 145 1 Reads read VBZ cord-258014-lzzi4rnz 145 2 matching match VBG cord-258014-lzzi4rnz 145 3 chloroplast chloroplast NN cord-258014-lzzi4rnz 145 4 , , , cord-258014-lzzi4rnz 145 5 mitochondria mitochondrion NNS cord-258014-lzzi4rnz 145 6 , , , cord-258014-lzzi4rnz 145 7 and and CC cord-258014-lzzi4rnz 145 8 unassigned unassigned JJ cord-258014-lzzi4rnz 145 9 sequences sequence NNS cord-258014-lzzi4rnz 145 10 should should MD cord-258014-lzzi4rnz 145 11 be be VB cord-258014-lzzi4rnz 145 12 removed remove VBN cord-258014-lzzi4rnz 145 13 as as RB cord-258014-lzzi4rnz 145 14 well well RB cord-258014-lzzi4rnz 145 15 as as IN cord-258014-lzzi4rnz 145 16 rare rare JJ cord-258014-lzzi4rnz 145 17 singletons singleton NNS cord-258014-lzzi4rnz 145 18 . . . cord-258014-lzzi4rnz 146 1 6 6 LS cord-258014-lzzi4rnz 146 2 . . . cord-258014-lzzi4rnz 147 1 Downstream downstream JJ cord-258014-lzzi4rnz 147 2 taxonomy taxonomy NN cord-258014-lzzi4rnz 147 3 plots plot NNS cord-258014-lzzi4rnz 147 4 , , , cord-258014-lzzi4rnz 147 5 alpha alpha NN cord-258014-lzzi4rnz 147 6 and and CC cord-258014-lzzi4rnz 147 7 beta beta NN cord-258014-lzzi4rnz 147 8 analyses analysis NNS cord-258014-lzzi4rnz 147 9 should should MD cord-258014-lzzi4rnz 147 10 follow follow VB cord-258014-lzzi4rnz 147 11 a a DT cord-258014-lzzi4rnz 147 12 given give VBN cord-258014-lzzi4rnz 147 13 rarefaction rarefaction NN cord-258014-lzzi4rnz 147 14 level level NN cord-258014-lzzi4rnz 147 15 considering consider VBG cord-258014-lzzi4rnz 147 16 a a DT cord-258014-lzzi4rnz 147 17 minimum minimum JJ cord-258014-lzzi4rnz 147 18 number number NN cord-258014-lzzi4rnz 147 19 of of IN cord-258014-lzzi4rnz 147 20 reads read NNS cord-258014-lzzi4rnz 147 21 shared share VBN cord-258014-lzzi4rnz 147 22 by by IN cord-258014-lzzi4rnz 147 23 most most JJS cord-258014-lzzi4rnz 147 24 samples sample NNS cord-258014-lzzi4rnz 147 25 . . . cord-258014-lzzi4rnz 148 1 7 7 CD cord-258014-lzzi4rnz 148 2 . . . cord-258014-lzzi4rnz 149 1 Taxonomic taxonomic JJ cord-258014-lzzi4rnz 149 2 barplots barplot NNS cord-258014-lzzi4rnz 149 3 , , , cord-258014-lzzi4rnz 149 4 alpha alpha NN cord-258014-lzzi4rnz 149 5 richness richness NN cord-258014-lzzi4rnz 149 6 ( ( -LRB- cord-258014-lzzi4rnz 149 7 number number NN cord-258014-lzzi4rnz 149 8 of of IN cord-258014-lzzi4rnz 149 9 OTUs OTUs NNPS cord-258014-lzzi4rnz 149 10 / / SYM cord-258014-lzzi4rnz 149 11 Operational Operational NNP cord-258014-lzzi4rnz 149 12 Taxonomic Taxonomic NNP cord-258014-lzzi4rnz 149 13 Units Units NNPS cord-258014-lzzi4rnz 149 14 ) ) -RRB- cord-258014-lzzi4rnz 149 15 and and CC cord-258014-lzzi4rnz 149 16 geometric geometric JJ cord-258014-lzzi4rnz 149 17 diversity diversity NN cord-258014-lzzi4rnz 149 18 boxplots boxplot NNS cord-258014-lzzi4rnz 149 19 ( ( -LRB- cord-258014-lzzi4rnz 149 20 Shannon Shannon NNP cord-258014-lzzi4rnz 149 21 index index NN cord-258014-lzzi4rnz 149 22 of of IN cord-258014-lzzi4rnz 149 23 equitability equitability NN cord-258014-lzzi4rnz 149 24 [ [ -LRB- cord-258014-lzzi4rnz 149 25 31 31 CD cord-258014-lzzi4rnz 149 26 ] ] -RRB- cord-258014-lzzi4rnz 149 27 ) ) -RRB- cord-258014-lzzi4rnz 149 28 , , , cord-258014-lzzi4rnz 149 29 can can MD cord-258014-lzzi4rnz 149 30 be be VB cord-258014-lzzi4rnz 149 31 built build VBN cord-258014-lzzi4rnz 149 32 using use VBG cord-258014-lzzi4rnz 149 33 R R NNP cord-258014-lzzi4rnz 149 34 's 's POS cord-258014-lzzi4rnz 149 35 ggplot ggplot NN cord-258014-lzzi4rnz 149 36 package package NN cord-258014-lzzi4rnz 149 37 [ [ -LRB- cord-258014-lzzi4rnz 149 38 32 32 CD cord-258014-lzzi4rnz 149 39 ] ] -RRB- cord-258014-lzzi4rnz 149 40 . . . cord-258014-lzzi4rnz 150 1 8 8 CD cord-258014-lzzi4rnz 150 2 . . . cord-258014-lzzi4rnz 151 1 Heatmaps Heatmaps NNP cord-258014-lzzi4rnz 151 2 can can MD cord-258014-lzzi4rnz 151 3 be be VB cord-258014-lzzi4rnz 151 4 built build VBN cord-258014-lzzi4rnz 151 5 using use VBG cord-258014-lzzi4rnz 151 6 the the DT cord-258014-lzzi4rnz 151 7 plot_heatmap plot_heatmap XX cord-258014-lzzi4rnz 151 8 function function NN cord-258014-lzzi4rnz 151 9 from from IN cord-258014-lzzi4rnz 151 10 the the DT cord-258014-lzzi4rnz 151 11 heatmap heatmap NNP cord-258014-lzzi4rnz 151 12 library library NN cord-258014-lzzi4rnz 151 13 [ [ -LRB- cord-258014-lzzi4rnz 151 14 33 33 CD cord-258014-lzzi4rnz 151 15 ] ] -RRB- cord-258014-lzzi4rnz 151 16 . . . cord-258014-lzzi4rnz 152 1 9 9 LS cord-258014-lzzi4rnz 152 2 . . . cord-258014-lzzi4rnz 153 1 Additional additional JJ cord-258014-lzzi4rnz 153 2 analyses analysis NNS cord-258014-lzzi4rnz 153 3 can can MD cord-258014-lzzi4rnz 153 4 be be VB cord-258014-lzzi4rnz 153 5 plotted plot VBN cord-258014-lzzi4rnz 153 6 using use VBG cord-258014-lzzi4rnz 153 7 a a DT cord-258014-lzzi4rnz 153 8 microbiomeanalyst microbiomeanalyst NN cord-258014-lzzi4rnz 153 9 that that WDT cord-258014-lzzi4rnz 153 10 integrates integrate VBZ cord-258014-lzzi4rnz 153 11 multiple multiple JJ cord-258014-lzzi4rnz 153 12 R r NN cord-258014-lzzi4rnz 153 13 programs program NNS cord-258014-lzzi4rnz 153 14 [ [ -LRB- cord-258014-lzzi4rnz 153 15 30 30 CD cord-258014-lzzi4rnz 153 16 ] ] -RRB- cord-258014-lzzi4rnz 153 17 and and CC cord-258014-lzzi4rnz 153 18 also also RB cord-258014-lzzi4rnz 153 19 includes include VBZ cord-258014-lzzi4rnz 153 20 LEfSe lefse XX cord-258014-lzzi4rnz 153 21 ( ( -LRB- cord-258014-lzzi4rnz 153 22 Linear linear JJ cord-258014-lzzi4rnz 153 23 discriminant discriminant JJ cord-258014-lzzi4rnz 153 24 analysis analysis NN cord-258014-lzzi4rnz 153 25 effect effect NN cord-258014-lzzi4rnz 153 26 size size NN cord-258014-lzzi4rnz 153 27 ) ) -RRB- cord-258014-lzzi4rnz 153 28 analyses analysis NNS cord-258014-lzzi4rnz 153 29 [ [ -LRB- cord-258014-lzzi4rnz 153 30 34 34 CD cord-258014-lzzi4rnz 153 31 ] ] -RRB- cord-258014-lzzi4rnz 153 32 . . . cord-258014-lzzi4rnz 154 1 10 10 CD cord-258014-lzzi4rnz 154 2 . . . cord-258014-lzzi4rnz 155 1 For for IN cord-258014-lzzi4rnz 155 2 beta beta JJ cord-258014-lzzi4rnz 155 3 diversity diversity NN cord-258014-lzzi4rnz 155 4 , , , cord-258014-lzzi4rnz 155 5 samples sample NNS cord-258014-lzzi4rnz 155 6 can can MD cord-258014-lzzi4rnz 155 7 be be VB cord-258014-lzzi4rnz 155 8 visualized visualize VBN cord-258014-lzzi4rnz 155 9 using use VBG cord-258014-lzzi4rnz 155 10 pairwise pairwise JJ cord-258014-lzzi4rnz 155 11 Bray Bray NNP cord-258014-lzzi4rnz 155 12 - - HYPH cord-258014-lzzi4rnz 155 13 Curtis Curtis NNP cord-258014-lzzi4rnz 155 14 distance distance NN cord-258014-lzzi4rnz 155 15 between between IN cord-258014-lzzi4rnz 155 16 samples sample NNS cord-258014-lzzi4rnz 155 17 using use VBG cord-258014-lzzi4rnz 155 18 the the DT cord-258014-lzzi4rnz 155 19 R r NN cord-258014-lzzi4rnz 155 20 package package NN cord-258014-lzzi4rnz 155 21 [ [ -LRB- cord-258014-lzzi4rnz 155 22 35 35 CD cord-258014-lzzi4rnz 155 23 ] ] -RRB- cord-258014-lzzi4rnz 155 24 with with IN cord-258014-lzzi4rnz 155 25 vegdist vegdist NN cord-258014-lzzi4rnz 155 26 function function NN cord-258014-lzzi4rnz 155 27 in in IN cord-258014-lzzi4rnz 155 28 vegan vegan JJ cord-258014-lzzi4rnz 155 29 [ [ -LRB- cord-258014-lzzi4rnz 155 30 36 36 CD cord-258014-lzzi4rnz 155 31 ] ] -RRB- cord-258014-lzzi4rnz 155 32 . . . cord-258014-lzzi4rnz 156 1 The the DT cord-258014-lzzi4rnz 156 2 global global JJ cord-258014-lzzi4rnz 156 3 differences difference NNS cord-258014-lzzi4rnz 156 4 in in IN cord-258014-lzzi4rnz 156 5 bacteria bacteria NNS cord-258014-lzzi4rnz 156 6 can can MD cord-258014-lzzi4rnz 156 7 be be VB cord-258014-lzzi4rnz 156 8 visualized visualize VBN cord-258014-lzzi4rnz 156 9 with with IN cord-258014-lzzi4rnz 156 10 Principal Principal NNP cord-258014-lzzi4rnz 156 11 Coordinates Coordinates NNP cord-258014-lzzi4rnz 156 12 Analysis Analysis NNP cord-258014-lzzi4rnz 156 13 ( ( -LRB- cord-258014-lzzi4rnz 156 14 PCoA PCoA NNP cord-258014-lzzi4rnz 156 15 ) ) -RRB- cord-258014-lzzi4rnz 156 16 or or CC cord-258014-lzzi4rnz 156 17 non non JJ cord-258014-lzzi4rnz 156 18 - - JJ cord-258014-lzzi4rnz 156 19 metric metric JJ cord-258014-lzzi4rnz 156 20 multidimensional multidimensional JJ cord-258014-lzzi4rnz 156 21 scaling scaling NN cord-258014-lzzi4rnz 156 22 ( ( -LRB- cord-258014-lzzi4rnz 156 23 NMDS NMDS NNP cord-258014-lzzi4rnz 156 24 ) ) -RRB- cord-258014-lzzi4rnz 156 25 . . . cord-258014-lzzi4rnz 157 1 Alternatively alternatively RB cord-258014-lzzi4rnz 157 2 , , , cord-258014-lzzi4rnz 157 3 beta beta JJ cord-258014-lzzi4rnz 157 4 diversity diversity NN cord-258014-lzzi4rnz 157 5 can can MD cord-258014-lzzi4rnz 157 6 be be VB cord-258014-lzzi4rnz 157 7 visualized visualize VBN cord-258014-lzzi4rnz 157 8 using use VBG cord-258014-lzzi4rnz 157 9 UniFRAC UniFRAC NNP cord-258014-lzzi4rnz 157 10 [ [ -LRB- cord-258014-lzzi4rnz 157 11 37 37 CD cord-258014-lzzi4rnz 157 12 ] ] -RRB- cord-258014-lzzi4rnz 157 13 . . . cord-258014-lzzi4rnz 158 1 All all DT cord-258014-lzzi4rnz 158 2 reagents reagent NNS cord-258014-lzzi4rnz 158 3 must must MD cord-258014-lzzi4rnz 158 4 be be VB cord-258014-lzzi4rnz 158 5 an an DT cord-258014-lzzi4rnz 158 6 analytical analytical JJ cord-258014-lzzi4rnz 158 7 grade grade NN cord-258014-lzzi4rnz 158 8 ( ( -LRB- cord-258014-lzzi4rnz 158 9 HPLC HPLC NNP cord-258014-lzzi4rnz 158 10 ) ) -RRB- cord-258014-lzzi4rnz 158 11 . . . cord-258014-lzzi4rnz 159 1 All all DT cord-258014-lzzi4rnz 159 2 aqueous aqueous JJ cord-258014-lzzi4rnz 159 3 solutions solution NNS cord-258014-lzzi4rnz 159 4 used use VBN cord-258014-lzzi4rnz 159 5 throughout throughout IN cord-258014-lzzi4rnz 159 6 this this DT cord-258014-lzzi4rnz 159 7 protocol protocol NN cord-258014-lzzi4rnz 159 8 should should MD cord-258014-lzzi4rnz 159 9 be be VB cord-258014-lzzi4rnz 159 10 prepared prepare VBN cord-258014-lzzi4rnz 159 11 with with IN cord-258014-lzzi4rnz 159 12 Milli Milli NNP cord-258014-lzzi4rnz 159 13 - - HYPH cord-258014-lzzi4rnz 159 14 Q Q NNP cord-258014-lzzi4rnz 159 15 or or CC cord-258014-lzzi4rnz 159 16 deionized deionize VBN cord-258014-lzzi4rnz 159 17 water water NN cord-258014-lzzi4rnz 159 18 ( ( -LRB- cord-258014-lzzi4rnz 159 19 18.2 18.2 CD cord-258014-lzzi4rnz 159 20 MΩ-cm mω-cm NN cord-258014-lzzi4rnz 159 21 , , , cord-258014-lzzi4rnz 159 22 at at IN cord-258014-lzzi4rnz 159 23 4 4 CD cord-258014-lzzi4rnz 159 24 • • NNP cord-258014-lzzi4rnz 159 25 ) ) -RRB- cord-258014-lzzi4rnz 159 26 . . . cord-258014-lzzi4rnz 160 1 When when WRB cord-258014-lzzi4rnz 160 2 possible possible JJ cord-258014-lzzi4rnz 160 3 , , , cord-258014-lzzi4rnz 160 4 all all DT cord-258014-lzzi4rnz 160 5 procedures procedure NNS cord-258014-lzzi4rnz 160 6 should should MD cord-258014-lzzi4rnz 160 7 be be VB cord-258014-lzzi4rnz 160 8 performed perform VBN cord-258014-lzzi4rnz 160 9 using use VBG cord-258014-lzzi4rnz 160 10 glass glass NN cord-258014-lzzi4rnz 160 11 appliances appliance NNS cord-258014-lzzi4rnz 160 12 . . . cord-258014-lzzi4rnz 161 1 The the DT cord-258014-lzzi4rnz 161 2 whole whole JJ cord-258014-lzzi4rnz 161 3 extraction extraction NN cord-258014-lzzi4rnz 161 4 needs need VBZ cord-258014-lzzi4rnz 161 5 to to TO cord-258014-lzzi4rnz 161 6 be be VB cord-258014-lzzi4rnz 161 7 performed perform VBN cord-258014-lzzi4rnz 161 8 at at IN cord-258014-lzzi4rnz 161 9 4 4 CD cord-258014-lzzi4rnz 161 10 • • NN cord-258014-lzzi4rnz 161 11 C C NNP cord-258014-lzzi4rnz 161 12 since since IN cord-258014-lzzi4rnz 161 13 the the DT cord-258014-lzzi4rnz 161 14 metabolites metabolite NNS cord-258014-lzzi4rnz 161 15 can can MD cord-258014-lzzi4rnz 161 16 degrade degrade VB cord-258014-lzzi4rnz 161 17 as as RB cord-258014-lzzi4rnz 161 18 little little JJ cord-258014-lzzi4rnz 161 19 as as IN cord-258014-lzzi4rnz 161 20 a a DT cord-258014-lzzi4rnz 161 21 few few JJ cord-258014-lzzi4rnz 161 22 seconds second NNS cord-258014-lzzi4rnz 161 23 . . . cord-258014-lzzi4rnz 162 1 Mix mix VB cord-258014-lzzi4rnz 162 2 with with IN cord-258014-lzzi4rnz 162 3 800 800 CD cord-258014-lzzi4rnz 162 4 µL µl CD cord-258014-lzzi4rnz 162 5 of of IN cord-258014-lzzi4rnz 162 6 the the DT cord-258014-lzzi4rnz 162 7 cold cold JJ cord-258014-lzzi4rnz 162 8 methanol methanol NN cord-258014-lzzi4rnz 162 9 - - HYPH cord-258014-lzzi4rnz 162 10 water water NN cord-258014-lzzi4rnz 162 11 mixture mixture NN cord-258014-lzzi4rnz 162 12 ( ( -LRB- cord-258014-lzzi4rnz 162 13 8:1 8:1 CD cord-258014-lzzi4rnz 162 14 v v NN cord-258014-lzzi4rnz 162 15 / / SYM cord-258014-lzzi4rnz 162 16 v v NN cord-258014-lzzi4rnz 162 17 ) ) -RRB- cord-258014-lzzi4rnz 162 18 3 3 CD cord-258014-lzzi4rnz 162 19 . . . cord-258014-lzzi4rnz 163 1 Vortex vortex NN cord-258014-lzzi4rnz 163 2 mixture mixture NN cord-258014-lzzi4rnz 163 3 for for IN cord-258014-lzzi4rnz 163 4 1 1 CD cord-258014-lzzi4rnz 163 5 min min NN cord-258014-lzzi4rnz 163 6 . . . cord-258014-lzzi4rnz 164 1 Keep keep VB cord-258014-lzzi4rnz 164 2 the the DT cord-258014-lzzi4rnz 164 3 sample sample NN cord-258014-lzzi4rnz 164 4 at at IN cord-258014-lzzi4rnz 164 5 4 4 CD cord-258014-lzzi4rnz 164 6 • • NN cord-258014-lzzi4rnz 164 7 C c NN cord-258014-lzzi4rnz 164 8 or or CC cord-258014-lzzi4rnz 164 9 ice ice NN cord-258014-lzzi4rnz 164 10 for for IN cord-258014-lzzi4rnz 164 11 20 20 CD cord-258014-lzzi4rnz 164 12 min min NN cord-258014-lzzi4rnz 164 13 . . . cord-258014-lzzi4rnz 165 1 Centrifuge centrifuge NN cord-258014-lzzi4rnz 165 2 at at IN cord-258014-lzzi4rnz 165 3 6000 6000 CD cord-258014-lzzi4rnz 165 4 rpm rpm NN cord-258014-lzzi4rnz 165 5 for for IN cord-258014-lzzi4rnz 165 6 8 8 CD cord-258014-lzzi4rnz 165 7 min min NN cord-258014-lzzi4rnz 165 8 at at IN cord-258014-lzzi4rnz 165 9 4 4 CD cord-258014-lzzi4rnz 166 1 • • NNP cord-258014-lzzi4rnz 166 2 C. C. NNP cord-258014-lzzi4rnz 166 3 6 6 CD cord-258014-lzzi4rnz 166 4 . . . cord-258014-lzzi4rnz 167 1 De De NNP cord-258014-lzzi4rnz 167 2 - - NN cord-258014-lzzi4rnz 167 3 assemble assemble VB cord-258014-lzzi4rnz 167 4 the the DT cord-258014-lzzi4rnz 167 5 screw screw NN cord-258014-lzzi4rnz 167 6 cap cap NN cord-258014-lzzi4rnz 167 7 and and CC cord-258014-lzzi4rnz 167 8 remove remove VB cord-258014-lzzi4rnz 167 9 silicone silicone NN cord-258014-lzzi4rnz 167 10 septum septum NN cord-258014-lzzi4rnz 167 11 . . . cord-258014-lzzi4rnz 168 1 Perforate perforate VB cord-258014-lzzi4rnz 168 2 it -PRON- PRP cord-258014-lzzi4rnz 168 3 using use VBG cord-258014-lzzi4rnz 168 4 scissors scissor NNS cord-258014-lzzi4rnz 168 5 and and CC cord-258014-lzzi4rnz 168 6 mount mount VB cord-258014-lzzi4rnz 168 7 vials vial NNS cord-258014-lzzi4rnz 168 8 onto onto IN cord-258014-lzzi4rnz 168 9 a a DT cord-258014-lzzi4rnz 168 10 rotary rotary JJ cord-258014-lzzi4rnz 168 11 vacuum vacuum NN cord-258014-lzzi4rnz 168 12 evaporator evaporator NN cord-258014-lzzi4rnz 168 13 . . . cord-258014-lzzi4rnz 169 1 ( ( -LRB- cord-258014-lzzi4rnz 169 2 see see VB cord-258014-lzzi4rnz 169 3 Note Note NNP cord-258014-lzzi4rnz 169 4 3 3 CD cord-258014-lzzi4rnz 169 5 ) ) -RRB- cord-258014-lzzi4rnz 169 6 . . . cord-258014-lzzi4rnz 170 1 7 7 CD cord-258014-lzzi4rnz 170 2 . . . cord-258014-lzzi4rnz 171 1 Remove remove VB cord-258014-lzzi4rnz 171 2 200 200 CD cord-258014-lzzi4rnz 171 3 µL µl CD cord-258014-lzzi4rnz 171 4 of of IN cord-258014-lzzi4rnz 171 5 the the DT cord-258014-lzzi4rnz 171 6 supernatant supernatant NN cord-258014-lzzi4rnz 171 7 from from IN cord-258014-lzzi4rnz 171 8 the the DT cord-258014-lzzi4rnz 171 9 top top JJ cord-258014-lzzi4rnz 171 10 phase phase NN cord-258014-lzzi4rnz 171 11 and and CC cord-258014-lzzi4rnz 171 12 transfer transfer VB cord-258014-lzzi4rnz 171 13 to to IN cord-258014-lzzi4rnz 171 14 a a DT cord-258014-lzzi4rnz 171 15 glass glass NN cord-258014-lzzi4rnz 171 16 vials vial NNS cord-258014-lzzi4rnz 171 17 , , , cord-258014-lzzi4rnz 171 18 thread thread NN cord-258014-lzzi4rnz 171 19 8 8 CD cord-258014-lzzi4rnz 171 20 - - SYM cord-258014-lzzi4rnz 171 21 425 425 CD cord-258014-lzzi4rnz 171 22 . . . cord-258014-lzzi4rnz 172 1 8 8 CD cord-258014-lzzi4rnz 172 2 . . . cord-258014-lzzi4rnz 173 1 Prepare prepare VB cord-258014-lzzi4rnz 173 2 reference reference NN cord-258014-lzzi4rnz 173 3 pool pool NN cord-258014-lzzi4rnz 173 4 quality quality NN cord-258014-lzzi4rnz 173 5 control control NN cord-258014-lzzi4rnz 173 6 samples sample NNS cord-258014-lzzi4rnz 173 7 ( ( -LRB- cord-258014-lzzi4rnz 173 8 see see VB cord-258014-lzzi4rnz 173 9 Note Note NNP cord-258014-lzzi4rnz 173 10 4 4 CD cord-258014-lzzi4rnz 173 11 ) ) -RRB- cord-258014-lzzi4rnz 173 12 . . . cord-258014-lzzi4rnz 174 1 9 9 LS cord-258014-lzzi4rnz 174 2 . . . cord-258014-lzzi4rnz 175 1 Attach attach VB cord-258014-lzzi4rnz 175 2 vials vial NNS cord-258014-lzzi4rnz 175 3 to to IN cord-258014-lzzi4rnz 175 4 a a DT cord-258014-lzzi4rnz 175 5 rotary rotary JJ cord-258014-lzzi4rnz 175 6 vacuum vacuum NN cord-258014-lzzi4rnz 175 7 evaporator evaporator NN cord-258014-lzzi4rnz 175 8 ( ( -LRB- cord-258014-lzzi4rnz 175 9 see see VB cord-258014-lzzi4rnz 175 10 Note Note NNP cord-258014-lzzi4rnz 175 11 5 5 CD cord-258014-lzzi4rnz 175 12 ) ) -RRB- cord-258014-lzzi4rnz 175 13 . . . cord-258014-lzzi4rnz 176 1 10 10 CD cord-258014-lzzi4rnz 176 2 . . . cord-258014-lzzi4rnz 177 1 Evaporate evaporate VB cord-258014-lzzi4rnz 177 2 supernatants supernatant NNS cord-258014-lzzi4rnz 177 3 to to IN cord-258014-lzzi4rnz 177 4 dryness dryness NN cord-258014-lzzi4rnz 177 5 . . . cord-258014-lzzi4rnz 178 1 11 11 CD cord-258014-lzzi4rnz 178 2 . . . cord-258014-lzzi4rnz 179 1 Replace replace VB cord-258014-lzzi4rnz 179 2 caps cap NNS cord-258014-lzzi4rnz 179 3 with with IN cord-258014-lzzi4rnz 179 4 solid solid JJ cord-258014-lzzi4rnz 179 5 tops top NNS cord-258014-lzzi4rnz 179 6 . . . cord-258014-lzzi4rnz 180 1 12 12 CD cord-258014-lzzi4rnz 180 2 . . . cord-258014-lzzi4rnz 181 1 Put put VB cord-258014-lzzi4rnz 181 2 vials vial NNS cord-258014-lzzi4rnz 181 3 in in IN cord-258014-lzzi4rnz 181 4 desiccator desiccator NN cord-258014-lzzi4rnz 181 5 and and CC cord-258014-lzzi4rnz 181 6 store store NN cord-258014-lzzi4rnz 181 7 at at IN cord-258014-lzzi4rnz 181 8 −80 −80 NNP cord-258014-lzzi4rnz 182 1 • • NNP cord-258014-lzzi4rnz 183 1 C C NNP cord-258014-lzzi4rnz 183 2 for for IN cord-258014-lzzi4rnz 183 3 at at RB cord-258014-lzzi4rnz 183 4 least least RBS cord-258014-lzzi4rnz 183 5 four four CD cord-258014-lzzi4rnz 183 6 weeks week NNS cord-258014-lzzi4rnz 183 7 . . . cord-258014-lzzi4rnz 184 1 Prepare prepare VB cord-258014-lzzi4rnz 184 2 the the DT cord-258014-lzzi4rnz 184 3 derivatization derivatization NN cord-258014-lzzi4rnz 184 4 solution solution NN cord-258014-lzzi4rnz 184 5 1 1 CD cord-258014-lzzi4rnz 184 6 : : : cord-258014-lzzi4rnz 184 7 mix mix VB cord-258014-lzzi4rnz 184 8 20 20 CD cord-258014-lzzi4rnz 184 9 mg mg NNP cord-258014-lzzi4rnz 184 10 Methoxyamine Methoxyamine NNP cord-258014-lzzi4rnz 184 11 hydrochloride hydrochloride NN cord-258014-lzzi4rnz 184 12 in in IN cord-258014-lzzi4rnz 184 13 1 1 CD cord-258014-lzzi4rnz 184 14 mL ml NN cord-258014-lzzi4rnz 184 15 pyridine pyridine NN cord-258014-lzzi4rnz 184 16 using use VBG cord-258014-lzzi4rnz 184 17 4 4 CD cord-258014-lzzi4rnz 184 18 mL ml NN cord-258014-lzzi4rnz 184 19 glass glass NN cord-258014-lzzi4rnz 184 20 vials vial NNS cord-258014-lzzi4rnz 184 21 with with IN cord-258014-lzzi4rnz 184 22 plastic plastic JJ cord-258014-lzzi4rnz 184 23 caps cap NNS cord-258014-lzzi4rnz 184 24 solid solid JJ cord-258014-lzzi4rnz 184 25 - - HYPH cord-258014-lzzi4rnz 184 26 top top NN cord-258014-lzzi4rnz 184 27 , , , cord-258014-lzzi4rnz 184 28 thread thread NN cord-258014-lzzi4rnz 184 29 13 13 CD cord-258014-lzzi4rnz 184 30 - - SYM cord-258014-lzzi4rnz 184 31 425 425 CD cord-258014-lzzi4rnz 184 32 ( ( -LRB- cord-258014-lzzi4rnz 184 33 see see VB cord-258014-lzzi4rnz 184 34 Note Note NNP cord-258014-lzzi4rnz 184 35 6 6 CD cord-258014-lzzi4rnz 184 36 ) ) -RRB- cord-258014-lzzi4rnz 184 37 . . . cord-258014-lzzi4rnz 185 1 Briefly briefly RB cord-258014-lzzi4rnz 185 2 , , , cord-258014-lzzi4rnz 185 3 vortex vortex VB cord-258014-lzzi4rnz 185 4 the the DT cord-258014-lzzi4rnz 185 5 obtained obtain VBN cord-258014-lzzi4rnz 185 6 solution solution NN cord-258014-lzzi4rnz 185 7 at at IN cord-258014-lzzi4rnz 185 8 RT RT NNP cord-258014-lzzi4rnz 185 9 until until IN cord-258014-lzzi4rnz 185 10 Methoxyamine Methoxyamine NNP cord-258014-lzzi4rnz 185 11 hydrochloride hydrochloride NN cord-258014-lzzi4rnz 185 12 is be VBZ cord-258014-lzzi4rnz 185 13 fully fully RB cord-258014-lzzi4rnz 185 14 dissolved dissolve VBN cord-258014-lzzi4rnz 185 15 . . . cord-258014-lzzi4rnz 186 1 Take take VB cord-258014-lzzi4rnz 186 2 out out RP cord-258014-lzzi4rnz 186 3 dried dry VBN cord-258014-lzzi4rnz 186 4 samples sample NNS cord-258014-lzzi4rnz 186 5 from from IN cord-258014-lzzi4rnz 186 6 storage storage NN cord-258014-lzzi4rnz 186 7 and and CC cord-258014-lzzi4rnz 186 8 allow allow VB cord-258014-lzzi4rnz 186 9 them -PRON- PRP cord-258014-lzzi4rnz 186 10 to to TO cord-258014-lzzi4rnz 186 11 warm warm VB cord-258014-lzzi4rnz 186 12 up up RP cord-258014-lzzi4rnz 186 13 to to IN cord-258014-lzzi4rnz 186 14 room room NN cord-258014-lzzi4rnz 186 15 temperature temperature NN cord-258014-lzzi4rnz 186 16 for for IN cord-258014-lzzi4rnz 186 17 at at RB cord-258014-lzzi4rnz 186 18 least least RBS cord-258014-lzzi4rnz 186 19 15 15 CD cord-258014-lzzi4rnz 186 20 min min NN cord-258014-lzzi4rnz 186 21 before before IN cord-258014-lzzi4rnz 186 22 derivatization derivatization NN cord-258014-lzzi4rnz 186 23 ( ( -LRB- cord-258014-lzzi4rnz 186 24 see see VB cord-258014-lzzi4rnz 186 25 Note Note NNP cord-258014-lzzi4rnz 186 26 7 7 CD cord-258014-lzzi4rnz 186 27 ) ) -RRB- cord-258014-lzzi4rnz 186 28 and and CC cord-258014-lzzi4rnz 186 29 add add VB cord-258014-lzzi4rnz 186 30 50 50 CD cord-258014-lzzi4rnz 186 31 µL µL NNP cord-258014-lzzi4rnz 186 32 Derivatization Derivatization NNP cord-258014-lzzi4rnz 186 33 Solution Solution NNP cord-258014-lzzi4rnz 186 34 1 1 CD cord-258014-lzzi4rnz 186 35 , , , cord-258014-lzzi4rnz 186 36 close close VBP cord-258014-lzzi4rnz 186 37 tightly tightly RB cord-258014-lzzi4rnz 186 38 the the DT cord-258014-lzzi4rnz 186 39 vials vial NNS cord-258014-lzzi4rnz 186 40 with with IN cord-258014-lzzi4rnz 186 41 closed closed JJ cord-258014-lzzi4rnz 186 42 caps cap NNS cord-258014-lzzi4rnz 186 43 and and CC cord-258014-lzzi4rnz 186 44 incubate incubate VB cord-258014-lzzi4rnz 186 45 at at IN cord-258014-lzzi4rnz 186 46 37 37 CD cord-258014-lzzi4rnz 186 47 • • NN cord-258014-lzzi4rnz 186 48 C c NN cord-258014-lzzi4rnz 186 49 for for IN cord-258014-lzzi4rnz 186 50 two two CD cord-258014-lzzi4rnz 186 51 hours hour NNS cord-258014-lzzi4rnz 186 52 . . . cord-258014-lzzi4rnz 187 1 Remove remove VB cord-258014-lzzi4rnz 187 2 samples sample NNS cord-258014-lzzi4rnz 187 3 , , , cord-258014-lzzi4rnz 187 4 add add VB cord-258014-lzzi4rnz 187 5 50 50 CD cord-258014-lzzi4rnz 187 6 µL µl CD cord-258014-lzzi4rnz 187 7 of of IN cord-258014-lzzi4rnz 187 8 the the DT cord-258014-lzzi4rnz 187 9 Derivatization Derivatization NNP cord-258014-lzzi4rnz 187 10 Solution Solution NNP cord-258014-lzzi4rnz 187 11 2 2 CD cord-258014-lzzi4rnz 187 12 ( ( -LRB- cord-258014-lzzi4rnz 187 13 see see VB cord-258014-lzzi4rnz 187 14 Note note NN cord-258014-lzzi4rnz 187 15 8) 8) NN cord-258014-lzzi4rnz 187 16 directly directly RB cord-258014-lzzi4rnz 187 17 to to IN cord-258014-lzzi4rnz 187 18 the the DT cord-258014-lzzi4rnz 187 19 reaction reaction NN cord-258014-lzzi4rnz 187 20 mixture mixture NN cord-258014-lzzi4rnz 187 21 in in IN cord-258014-lzzi4rnz 187 22 the the DT cord-258014-lzzi4rnz 187 23 vial vial NN cord-258014-lzzi4rnz 187 24 , , , cord-258014-lzzi4rnz 187 25 close close VB cord-258014-lzzi4rnz 187 26 tightly tightly RB cord-258014-lzzi4rnz 187 27 the the DT cord-258014-lzzi4rnz 187 28 vials vial NNS cord-258014-lzzi4rnz 187 29 with with IN cord-258014-lzzi4rnz 187 30 closed closed JJ cord-258014-lzzi4rnz 187 31 caps cap NNS cord-258014-lzzi4rnz 187 32 and and CC cord-258014-lzzi4rnz 187 33 incubate incubate VB cord-258014-lzzi4rnz 187 34 for for IN cord-258014-lzzi4rnz 187 35 an an DT cord-258014-lzzi4rnz 187 36 additional additional JJ cord-258014-lzzi4rnz 187 37 1 1 CD cord-258014-lzzi4rnz 187 38 h h NN cord-258014-lzzi4rnz 187 39 at at IN cord-258014-lzzi4rnz 187 40 60 60 CD cord-258014-lzzi4rnz 187 41 • • NNP cord-258014-lzzi4rnz 187 42 C. C. NNP cord-258014-lzzi4rnz 187 43 Transfer transfer VB cord-258014-lzzi4rnz 187 44 the the DT cord-258014-lzzi4rnz 187 45 reaction reaction NN cord-258014-lzzi4rnz 187 46 mixture mixture NN cord-258014-lzzi4rnz 187 47 to to IN cord-258014-lzzi4rnz 187 48 labeled label VBN cord-258014-lzzi4rnz 187 49 Eppendorf Eppendorf NNP cord-258014-lzzi4rnz 187 50 vial vial NN cord-258014-lzzi4rnz 187 51 and and CC cord-258014-lzzi4rnz 187 52 centrifuge centrifuge NN cord-258014-lzzi4rnz 187 53 at at IN cord-258014-lzzi4rnz 187 54 13,000 13,000 CD cord-258014-lzzi4rnz 187 55 rpm rpm NN cord-258014-lzzi4rnz 187 56 for for IN cord-258014-lzzi4rnz 187 57 10 10 CD cord-258014-lzzi4rnz 187 58 min min NN cord-258014-lzzi4rnz 187 59 at at IN cord-258014-lzzi4rnz 187 60 RT RT NNP cord-258014-lzzi4rnz 187 61 . . . cord-258014-lzzi4rnz 188 1 Transfer transfer VB cord-258014-lzzi4rnz 188 2 the the DT cord-258014-lzzi4rnz 188 3 supernatant supernatant NN cord-258014-lzzi4rnz 188 4 in in IN cord-258014-lzzi4rnz 188 5 the the DT cord-258014-lzzi4rnz 188 6 new new JJ cord-258014-lzzi4rnz 188 7 glass glass NN cord-258014-lzzi4rnz 188 8 vial vial NN cord-258014-lzzi4rnz 188 9 and and CC cord-258014-lzzi4rnz 188 10 immediately immediately RB cord-258014-lzzi4rnz 188 11 close close VB cord-258014-lzzi4rnz 188 12 each each DT cord-258014-lzzi4rnz 188 13 sample sample NN cord-258014-lzzi4rnz 188 14 with with IN cord-258014-lzzi4rnz 188 15 a a DT cord-258014-lzzi4rnz 188 16 solid solid JJ cord-258014-lzzi4rnz 188 17 plastic plastic NN cord-258014-lzzi4rnz 188 18 cap cap NN cord-258014-lzzi4rnz 188 19 , , , cord-258014-lzzi4rnz 188 20 thread thread NN cord-258014-lzzi4rnz 188 21 8 8 CD cord-258014-lzzi4rnz 188 22 - - SYM cord-258014-lzzi4rnz 188 23 425 425 CD cord-258014-lzzi4rnz 188 24 . . . cord-258014-lzzi4rnz 189 1 Store store VB cord-258014-lzzi4rnz 189 2 for for IN cord-258014-lzzi4rnz 189 3 a a DT cord-258014-lzzi4rnz 189 4 long long JJ cord-258014-lzzi4rnz 189 5 time time NN cord-258014-lzzi4rnz 189 6 at at IN cord-258014-lzzi4rnz 189 7 −20 −20 NNP cord-258014-lzzi4rnz 189 8 • • NNP cord-258014-lzzi4rnz 189 9 C C NNP cord-258014-lzzi4rnz 189 10 and and CC cord-258014-lzzi4rnz 189 11 for for IN cord-258014-lzzi4rnz 189 12 a a DT cord-258014-lzzi4rnz 189 13 short short JJ cord-258014-lzzi4rnz 189 14 time time NN cord-258014-lzzi4rnz 189 15 at at IN cord-258014-lzzi4rnz 189 16 4 4 CD cord-258014-lzzi4rnz 190 1 • • NNP cord-258014-lzzi4rnz 191 1 C C NNP cord-258014-lzzi4rnz 191 2 ( ( -LRB- cord-258014-lzzi4rnz 191 3 see see VB cord-258014-lzzi4rnz 191 4 Note Note NNP cord-258014-lzzi4rnz 191 5 9 9 CD cord-258014-lzzi4rnz 191 6 ) ) -RRB- cord-258014-lzzi4rnz 191 7 . . . cord-258014-lzzi4rnz 192 1 6 6 LS cord-258014-lzzi4rnz 192 2 . . . cord-258014-lzzi4rnz 193 1 For for IN cord-258014-lzzi4rnz 193 2 the the DT cord-258014-lzzi4rnz 193 3 analysis analysis NN cord-258014-lzzi4rnz 193 4 , , , cord-258014-lzzi4rnz 193 5 place place VB cord-258014-lzzi4rnz 193 6 a a DT cord-258014-lzzi4rnz 193 7 glass glass NN cord-258014-lzzi4rnz 193 8 insert insert NN cord-258014-lzzi4rnz 193 9 in in IN cord-258014-lzzi4rnz 193 10 the the DT cord-258014-lzzi4rnz 193 11 new new JJ cord-258014-lzzi4rnz 193 12 glass glass NN cord-258014-lzzi4rnz 193 13 vial vial NN cord-258014-lzzi4rnz 193 14 and and CC cord-258014-lzzi4rnz 193 15 dilute dilute VB cord-258014-lzzi4rnz 193 16 each each DT cord-258014-lzzi4rnz 193 17 sample sample NN cord-258014-lzzi4rnz 193 18 1:50 1:50 CD cord-258014-lzzi4rnz 193 19 with with IN cord-258014-lzzi4rnz 193 20 hexane hexane NN cord-258014-lzzi4rnz 193 21 prior prior RB cord-258014-lzzi4rnz 193 22 to to IN cord-258014-lzzi4rnz 193 23 GC GC NNP cord-258014-lzzi4rnz 193 24 / / SYM cord-258014-lzzi4rnz 193 25 MS MS NNP cord-258014-lzzi4rnz 193 26 analysis analysis NN cord-258014-lzzi4rnz 193 27 ( ( -LRB- cord-258014-lzzi4rnz 193 28 see see VB cord-258014-lzzi4rnz 193 29 Note Note NNP cord-258014-lzzi4rnz 193 30 10 10 CD cord-258014-lzzi4rnz 193 31 ) ) -RRB- cord-258014-lzzi4rnz 193 32 . . . cord-258014-lzzi4rnz 194 1 The the DT cord-258014-lzzi4rnz 194 2 mass mass NN cord-258014-lzzi4rnz 194 3 spectrometer spectrometer NN cord-258014-lzzi4rnz 194 4 must must MD cord-258014-lzzi4rnz 194 5 be be VB cord-258014-lzzi4rnz 194 6 tuned tune VBN cord-258014-lzzi4rnz 194 7 according accord VBG cord-258014-lzzi4rnz 194 8 to to IN cord-258014-lzzi4rnz 194 9 the the DT cord-258014-lzzi4rnz 194 10 manufacturer manufacturer NN cord-258014-lzzi4rnz 194 11 's 's POS cord-258014-lzzi4rnz 194 12 manuals manual NNS cord-258014-lzzi4rnz 194 13 for for IN cord-258014-lzzi4rnz 194 14 optimal optimal JJ cord-258014-lzzi4rnz 194 15 parameters parameter NNS cord-258014-lzzi4rnz 194 16 for for IN cord-258014-lzzi4rnz 194 17 ion ion NN cord-258014-lzzi4rnz 194 18 lenses lense NNS cord-258014-lzzi4rnz 194 19 , , , cord-258014-lzzi4rnz 194 20 detector detector NN cord-258014-lzzi4rnz 194 21 voltage voltage NN cord-258014-lzzi4rnz 194 22 , , , cord-258014-lzzi4rnz 194 23 and and CC cord-258014-lzzi4rnz 194 24 other other JJ cord-258014-lzzi4rnz 194 25 settings setting NNS cord-258014-lzzi4rnz 194 26 . . . cord-258014-lzzi4rnz 195 1 Usually usually RB cord-258014-lzzi4rnz 195 2 , , , cord-258014-lzzi4rnz 195 3 this this DT cord-258014-lzzi4rnz 195 4 can can MD cord-258014-lzzi4rnz 195 5 be be VB cord-258014-lzzi4rnz 195 6 performed perform VBN cord-258014-lzzi4rnz 195 7 in in IN cord-258014-lzzi4rnz 195 8 autotune autotune NN cord-258014-lzzi4rnz 195 9 operation operation NN cord-258014-lzzi4rnz 195 10 . . . cord-258014-lzzi4rnz 196 1 Inject inject VB cord-258014-lzzi4rnz 196 2 1 1 CD cord-258014-lzzi4rnz 196 3 µL µl CD cord-258014-lzzi4rnz 196 4 of of IN cord-258014-lzzi4rnz 196 5 each each DT cord-258014-lzzi4rnz 196 6 sample sample NN cord-258014-lzzi4rnz 196 7 including include VBG cord-258014-lzzi4rnz 196 8 quality quality NN cord-258014-lzzi4rnz 196 9 control control NN cord-258014-lzzi4rnz 196 10 samples sample NNS cord-258014-lzzi4rnz 196 11 in in IN cord-258014-lzzi4rnz 196 12 the the DT cord-258014-lzzi4rnz 196 13 GC GC NNP cord-258014-lzzi4rnz 196 14 - - HYPH cord-258014-lzzi4rnz 196 15 MS MS NNP cord-258014-lzzi4rnz 196 16 . . . cord-258014-lzzi4rnz 197 1 Separate separate JJ cord-258014-lzzi4rnz 197 2 metabolites metabolite NNS cord-258014-lzzi4rnz 197 3 using use VBG cord-258014-lzzi4rnz 197 4 a a DT cord-258014-lzzi4rnz 197 5 GC GC NNP cord-258014-lzzi4rnz 197 6 temperature temperature NN cord-258014-lzzi4rnz 197 7 ramping ramp VBG cord-258014-lzzi4rnz 197 8 program program NN cord-258014-lzzi4rnz 197 9 . . . cord-258014-lzzi4rnz 198 1 The the DT cord-258014-lzzi4rnz 198 2 GC GC NNP cord-258014-lzzi4rnz 198 3 oven oven NN cord-258014-lzzi4rnz 198 4 can can MD cord-258014-lzzi4rnz 198 5 be be VB cord-258014-lzzi4rnz 198 6 programmed program VBN cord-258014-lzzi4rnz 198 7 from from IN cord-258014-lzzi4rnz 198 8 100 100 CD cord-258014-lzzi4rnz 198 9 • • NNS cord-258014-lzzi4rnz 198 10 C c NN cord-258014-lzzi4rnz 198 11 to to IN cord-258014-lzzi4rnz 198 12 280 280 CD cord-258014-lzzi4rnz 199 1 • • NNP cord-258014-lzzi4rnz 200 1 C C NNP cord-258014-lzzi4rnz 200 2 at at IN cord-258014-lzzi4rnz 200 3 a a DT cord-258014-lzzi4rnz 200 4 rate rate NN cord-258014-lzzi4rnz 200 5 of of IN cord-258014-lzzi4rnz 200 6 4 4 CD cord-258014-lzzi4rnz 200 7 • • NN cord-258014-lzzi4rnz 200 8 C C NNP cord-258014-lzzi4rnz 200 9 / / SYM cord-258014-lzzi4rnz 200 10 min min NN cord-258014-lzzi4rnz 200 11 . . . cord-258014-lzzi4rnz 201 1 The the DT cord-258014-lzzi4rnz 201 2 injection injection NN cord-258014-lzzi4rnz 201 3 port port NN cord-258014-lzzi4rnz 201 4 temperature temperature NN cord-258014-lzzi4rnz 201 5 will will MD cord-258014-lzzi4rnz 201 6 be be VB cord-258014-lzzi4rnz 201 7 280 280 CD cord-258014-lzzi4rnz 201 8 • • NN cord-258014-lzzi4rnz 201 9 C c NN cord-258014-lzzi4rnz 201 10 , , , cord-258014-lzzi4rnz 201 11 and and CC cord-258014-lzzi4rnz 201 12 helium helium NN cord-258014-lzzi4rnz 201 13 is be VBZ cord-258014-lzzi4rnz 201 14 used use VBN cord-258014-lzzi4rnz 201 15 as as IN cord-258014-lzzi4rnz 201 16 the the DT cord-258014-lzzi4rnz 201 17 carrier carrier NN cord-258014-lzzi4rnz 201 18 gas gas NN cord-258014-lzzi4rnz 201 19 at at IN cord-258014-lzzi4rnz 201 20 a a DT cord-258014-lzzi4rnz 201 21 constant constant JJ cord-258014-lzzi4rnz 201 22 linear linear JJ cord-258014-lzzi4rnz 201 23 velocity velocity NN cord-258014-lzzi4rnz 201 24 of of IN cord-258014-lzzi4rnz 201 25 39 39 CD cord-258014-lzzi4rnz 201 26 cm cm NNS cord-258014-lzzi4rnz 201 27 / / SYM cord-258014-lzzi4rnz 201 28 s s NN cord-258014-lzzi4rnz 201 29 . . . cord-258014-lzzi4rnz 202 1 Injection injection NN cord-258014-lzzi4rnz 202 2 mode mode NN cord-258014-lzzi4rnz 202 3 - - HYPH cord-258014-lzzi4rnz 202 4 split split VBN cord-258014-lzzi4rnz 202 5 ( ( -LRB- cord-258014-lzzi4rnz 202 6 15:1 15:1 CD cord-258014-lzzi4rnz 202 7 ) ) -RRB- cord-258014-lzzi4rnz 202 8 . . . cord-258014-lzzi4rnz 203 1 Detect detect VB cord-258014-lzzi4rnz 203 2 metabolites metabolite NNS cord-258014-lzzi4rnz 203 3 by by IN cord-258014-lzzi4rnz 203 4 setting set VBG cord-258014-lzzi4rnz 203 5 the the DT cord-258014-lzzi4rnz 203 6 ion ion NN cord-258014-lzzi4rnz 203 7 source source NN cord-258014-lzzi4rnz 203 8 filament filament NN cord-258014-lzzi4rnz 203 9 energy energy NN cord-258014-lzzi4rnz 203 10 to to IN cord-258014-lzzi4rnz 203 11 70 70 CD cord-258014-lzzi4rnz 203 12 eV ev NN cord-258014-lzzi4rnz 203 13 , , , cord-258014-lzzi4rnz 203 14 the the DT cord-258014-lzzi4rnz 203 15 ion ion NN cord-258014-lzzi4rnz 203 16 source source NN cord-258014-lzzi4rnz 203 17 temperature temperature NN cord-258014-lzzi4rnz 203 18 −200 −200 NNP cord-258014-lzzi4rnz 203 19 • • NNP cord-258014-lzzi4rnz 203 20 C C NNP cord-258014-lzzi4rnz 203 21 , , , cord-258014-lzzi4rnz 203 22 and and CC cord-258014-lzzi4rnz 203 23 the the DT cord-258014-lzzi4rnz 203 24 scan scan JJ cord-258014-lzzi4rnz 203 25 range range NN cord-258014-lzzi4rnz 203 26 ( ( -LRB- cord-258014-lzzi4rnz 203 27 mass mass NN cord-258014-lzzi4rnz 203 28 - - HYPH cord-258014-lzzi4rnz 203 29 to to IN cord-258014-lzzi4rnz 203 30 - - HYPH cord-258014-lzzi4rnz 203 31 charge charge NN cord-258014-lzzi4rnz 203 32 , , , cord-258014-lzzi4rnz 203 33 m m NNP cord-258014-lzzi4rnz 203 34 / / SYM cord-258014-lzzi4rnz 203 35 z z NNP cord-258014-lzzi4rnz 203 36 ) ) -RRB- cord-258014-lzzi4rnz 203 37 35 35 CD cord-258014-lzzi4rnz 203 38 - - SYM cord-258014-lzzi4rnz 203 39 600 600 CD cord-258014-lzzi4rnz 203 40 Da Da NNS cord-258014-lzzi4rnz 203 41 . . . cord-258014-lzzi4rnz 204 1 Metabolomic Metabolomic NNP cord-258014-lzzi4rnz 204 2 Data Data NNP cord-258014-lzzi4rnz 204 3 Acquisition Acquisition NNP cord-258014-lzzi4rnz 204 4 and and CC cord-258014-lzzi4rnz 204 5 Analyses Analyses NNPS cord-258014-lzzi4rnz 204 6 Obtain obtain VBP cord-258014-lzzi4rnz 204 7 the the DT cord-258014-lzzi4rnz 204 8 total total JJ cord-258014-lzzi4rnz 204 9 ion ion NN cord-258014-lzzi4rnz 204 10 chromatogram chromatogram NNP cord-258014-lzzi4rnz 204 11 and and CC cord-258014-lzzi4rnz 204 12 mass mass NNP cord-258014-lzzi4rnz 204 13 chromatograms chromatogram NNS cord-258014-lzzi4rnz 204 14 for for IN cord-258014-lzzi4rnz 204 15 each each DT cord-258014-lzzi4rnz 204 16 detected detect VBN cord-258014-lzzi4rnz 204 17 metabolite metabolite NN cord-258014-lzzi4rnz 204 18 GC GC NNP cord-258014-lzzi4rnz 204 19 / / SYM cord-258014-lzzi4rnz 204 20 MS MS NNP cord-258014-lzzi4rnz 204 21 instruments instrument NNS cord-258014-lzzi4rnz 204 22 generate generate VBP cord-258014-lzzi4rnz 204 23 a a DT cord-258014-lzzi4rnz 204 24 single single JJ cord-258014-lzzi4rnz 204 25 file file NN cord-258014-lzzi4rnz 204 26 per per IN cord-258014-lzzi4rnz 204 27 sample sample NN cord-258014-lzzi4rnz 204 28 , , , cord-258014-lzzi4rnz 204 29 a a DT cord-258014-lzzi4rnz 204 30 list list NN cord-258014-lzzi4rnz 204 31 of of IN cord-258014-lzzi4rnz 204 32 mass mass JJ cord-258014-lzzi4rnz 204 33 spectra spectra NN cord-258014-lzzi4rnz 204 34 together together RB cord-258014-lzzi4rnz 204 35 with with IN cord-258014-lzzi4rnz 204 36 their -PRON- PRP$ cord-258014-lzzi4rnz 204 37 corresponding corresponding JJ cord-258014-lzzi4rnz 204 38 retention retention NN cord-258014-lzzi4rnz 204 39 times time NNS cord-258014-lzzi4rnz 204 40 ( ( -LRB- cord-258014-lzzi4rnz 204 41 RT RT NNP cord-258014-lzzi4rnz 204 42 ) ) -RRB- cord-258014-lzzi4rnz 204 43 . . . cord-258014-lzzi4rnz 205 1 These these DT cord-258014-lzzi4rnz 205 2 spectra spectrum NNS cord-258014-lzzi4rnz 205 3 are be VBP cord-258014-lzzi4rnz 205 4 commonly commonly RB cord-258014-lzzi4rnz 205 5 shown show VBN cord-258014-lzzi4rnz 205 6 on on IN cord-258014-lzzi4rnz 205 7 a a DT cord-258014-lzzi4rnz 205 8 chromatogram chromatogram NN cord-258014-lzzi4rnz 205 9 represented represent VBN cord-258014-lzzi4rnz 205 10 by by IN cord-258014-lzzi4rnz 205 11 RT RT NNP cord-258014-lzzi4rnz 205 12 on on IN cord-258014-lzzi4rnz 205 13 the the DT cord-258014-lzzi4rnz 205 14 horizontal horizontal JJ cord-258014-lzzi4rnz 205 15 axis axis NN cord-258014-lzzi4rnz 205 16 and and CC cord-258014-lzzi4rnz 205 17 signal signal JJ cord-258014-lzzi4rnz 205 18 intensity intensity NN cord-258014-lzzi4rnz 205 19 on on IN cord-258014-lzzi4rnz 205 20 the the DT cord-258014-lzzi4rnz 205 21 vertical vertical JJ cord-258014-lzzi4rnz 205 22 axis axis NN cord-258014-lzzi4rnz 205 23 ( ( -LRB- cord-258014-lzzi4rnz 205 24 Figure figure NN cord-258014-lzzi4rnz 205 25 2 2 CD cord-258014-lzzi4rnz 205 26 ) ) -RRB- cord-258014-lzzi4rnz 205 27 . . . cord-258014-lzzi4rnz 206 1 Identify identify VB cord-258014-lzzi4rnz 206 2 metabolites metabolite NNS cord-258014-lzzi4rnz 206 3 based base VBN cord-258014-lzzi4rnz 206 4 on on IN cord-258014-lzzi4rnz 206 5 the the DT cord-258014-lzzi4rnz 206 6 fragmentation fragmentation NN cord-258014-lzzi4rnz 206 7 pattern pattern NN cord-258014-lzzi4rnz 206 8 of of IN cord-258014-lzzi4rnz 206 9 molecular molecular JJ cord-258014-lzzi4rnz 206 10 ions ion NNS cord-258014-lzzi4rnz 206 11 ( ( -LRB- cord-258014-lzzi4rnz 206 12 characteristic characteristic JJ cord-258014-lzzi4rnz 206 13 fragment fragment NN cord-258014-lzzi4rnz 206 14 ions ion NNS cord-258014-lzzi4rnz 206 15 ) ) -RRB- cord-258014-lzzi4rnz 206 16 using use VBG cord-258014-lzzi4rnz 206 17 the the DT cord-258014-lzzi4rnz 206 18 GC GC NNP cord-258014-lzzi4rnz 206 19 / / SYM cord-258014-lzzi4rnz 206 20 MS MS NNP cord-258014-lzzi4rnz 206 21 manufacturer manufacturer NN cord-258014-lzzi4rnz 206 22 's 's POS cord-258014-lzzi4rnz 206 23 software software NN cord-258014-lzzi4rnz 206 24 equipped equip VBN cord-258014-lzzi4rnz 206 25 with with IN cord-258014-lzzi4rnz 206 26 NIST NIST NNP cord-258014-lzzi4rnz 206 27 / / SYM cord-258014-lzzi4rnz 206 28 EPA EPA NNP cord-258014-lzzi4rnz 206 29 / / SYM cord-258014-lzzi4rnz 206 30 NIH NIH NNP cord-258014-lzzi4rnz 206 31 Mass Mass NNP cord-258014-lzzi4rnz 206 32 spectral spectral JJ cord-258014-lzzi4rnz 206 33 Library Library NNP cord-258014-lzzi4rnz 206 34 ( ( -LRB- cord-258014-lzzi4rnz 206 35 NIST14 NIST14 NNP cord-258014-lzzi4rnz 206 36 ) ) -RRB- cord-258014-lzzi4rnz 207 1 ( ( -LRB- cord-258014-lzzi4rnz 207 2 see see VB cord-258014-lzzi4rnz 207 3 Note Note NNP cord-258014-lzzi4rnz 207 4 11 11 CD cord-258014-lzzi4rnz 207 5 ) ) -RRB- cord-258014-lzzi4rnz 207 6 . . . cord-258014-lzzi4rnz 208 1 GC GC NNP cord-258014-lzzi4rnz 208 2 / / SYM cord-258014-lzzi4rnz 208 3 MS MS NNP cord-258014-lzzi4rnz 208 4 instruments instrument NNS cord-258014-lzzi4rnz 208 5 generate generate VBP cord-258014-lzzi4rnz 208 6 a a DT cord-258014-lzzi4rnz 208 7 single single JJ cord-258014-lzzi4rnz 208 8 file file NN cord-258014-lzzi4rnz 208 9 per per IN cord-258014-lzzi4rnz 208 10 sample sample NN cord-258014-lzzi4rnz 208 11 , , , cord-258014-lzzi4rnz 208 12 a a DT cord-258014-lzzi4rnz 208 13 list list NN cord-258014-lzzi4rnz 208 14 of of IN cord-258014-lzzi4rnz 208 15 mass mass JJ cord-258014-lzzi4rnz 208 16 spectra spectra NN cord-258014-lzzi4rnz 208 17 together together RB cord-258014-lzzi4rnz 208 18 with with IN cord-258014-lzzi4rnz 208 19 their -PRON- PRP$ cord-258014-lzzi4rnz 208 20 corresponding corresponding JJ cord-258014-lzzi4rnz 208 21 retention retention NN cord-258014-lzzi4rnz 208 22 times time NNS cord-258014-lzzi4rnz 208 23 ( ( -LRB- cord-258014-lzzi4rnz 208 24 RT RT NNP cord-258014-lzzi4rnz 208 25 ) ) -RRB- cord-258014-lzzi4rnz 208 26 . . . cord-258014-lzzi4rnz 209 1 These these DT cord-258014-lzzi4rnz 209 2 spectra spectrum NNS cord-258014-lzzi4rnz 209 3 are be VBP cord-258014-lzzi4rnz 209 4 commonly commonly RB cord-258014-lzzi4rnz 209 5 shown show VBN cord-258014-lzzi4rnz 209 6 on on IN cord-258014-lzzi4rnz 209 7 a a DT cord-258014-lzzi4rnz 209 8 chromatogram chromatogram NN cord-258014-lzzi4rnz 209 9 represented represent VBN cord-258014-lzzi4rnz 209 10 by by IN cord-258014-lzzi4rnz 209 11 RT RT NNP cord-258014-lzzi4rnz 209 12 on on IN cord-258014-lzzi4rnz 209 13 the the DT cord-258014-lzzi4rnz 209 14 horizontal horizontal JJ cord-258014-lzzi4rnz 209 15 axis axis NN cord-258014-lzzi4rnz 209 16 and and CC cord-258014-lzzi4rnz 209 17 signal signal JJ cord-258014-lzzi4rnz 209 18 intensity intensity NN cord-258014-lzzi4rnz 209 19 on on IN cord-258014-lzzi4rnz 209 20 the the DT cord-258014-lzzi4rnz 209 21 vertical vertical JJ cord-258014-lzzi4rnz 209 22 axis axis NN cord-258014-lzzi4rnz 209 23 ( ( -LRB- cord-258014-lzzi4rnz 209 24 Figure figure NN cord-258014-lzzi4rnz 209 25 2 2 CD cord-258014-lzzi4rnz 209 26 ) ) -RRB- cord-258014-lzzi4rnz 209 27 . . . cord-258014-lzzi4rnz 210 1 2 2 LS cord-258014-lzzi4rnz 210 2 . . . cord-258014-lzzi4rnz 211 1 Identify identify VB cord-258014-lzzi4rnz 211 2 metabolites metabolite NNS cord-258014-lzzi4rnz 211 3 based base VBN cord-258014-lzzi4rnz 211 4 on on IN cord-258014-lzzi4rnz 211 5 the the DT cord-258014-lzzi4rnz 211 6 fragmentation fragmentation NN cord-258014-lzzi4rnz 211 7 pattern pattern NN cord-258014-lzzi4rnz 211 8 of of IN cord-258014-lzzi4rnz 211 9 molecular molecular JJ cord-258014-lzzi4rnz 211 10 ions ion NNS cord-258014-lzzi4rnz 211 11 ( ( -LRB- cord-258014-lzzi4rnz 211 12 characteristic characteristic JJ cord-258014-lzzi4rnz 211 13 fragment fragment NN cord-258014-lzzi4rnz 211 14 ions ion NNS cord-258014-lzzi4rnz 211 15 ) ) -RRB- cord-258014-lzzi4rnz 211 16 using use VBG cord-258014-lzzi4rnz 211 17 the the DT cord-258014-lzzi4rnz 211 18 GC GC NNP cord-258014-lzzi4rnz 211 19 / / SYM cord-258014-lzzi4rnz 211 20 MS MS NNP cord-258014-lzzi4rnz 211 21 manufacturer manufacturer NN cord-258014-lzzi4rnz 211 22 's 's POS cord-258014-lzzi4rnz 211 23 software software NN cord-258014-lzzi4rnz 211 24 equipped equip VBN cord-258014-lzzi4rnz 211 25 with with IN cord-258014-lzzi4rnz 211 26 NIST NIST NNP cord-258014-lzzi4rnz 211 27 / / SYM cord-258014-lzzi4rnz 211 28 EPA EPA NNP cord-258014-lzzi4rnz 211 29 / / SYM cord-258014-lzzi4rnz 211 30 NIH NIH NNP cord-258014-lzzi4rnz 211 31 Mass Mass NNP cord-258014-lzzi4rnz 211 32 spectral spectral JJ cord-258014-lzzi4rnz 211 33 Library Library NNP cord-258014-lzzi4rnz 211 34 ( ( -LRB- cord-258014-lzzi4rnz 211 35 NIST14 NIST14 NNP cord-258014-lzzi4rnz 211 36 ) ) -RRB- cord-258014-lzzi4rnz 212 1 ( ( -LRB- cord-258014-lzzi4rnz 212 2 see see VB cord-258014-lzzi4rnz 212 3 Note Note NNP cord-258014-lzzi4rnz 212 4 11 11 CD cord-258014-lzzi4rnz 212 5 ) ) -RRB- cord-258014-lzzi4rnz 212 6 . . . cord-258014-lzzi4rnz 212 7 _SP cord-258014-lzzi4rnz 213 1 Statistical statistical JJ cord-258014-lzzi4rnz 213 2 tests test NNS cord-258014-lzzi4rnz 213 3 on on IN cord-258014-lzzi4rnz 213 4 the the DT cord-258014-lzzi4rnz 213 5 beta beta NN cord-258014-lzzi4rnz 213 6 diversity diversity NN cord-258014-lzzi4rnz 213 7 can can MD cord-258014-lzzi4rnz 213 8 be be VB cord-258014-lzzi4rnz 213 9 completed complete VBN cord-258014-lzzi4rnz 213 10 via via IN cord-258014-lzzi4rnz 213 11 community community NN cord-258014-lzzi4rnz 213 12 - - HYPH cord-258014-lzzi4rnz 213 13 level level NN cord-258014-lzzi4rnz 213 14 differences difference NNS cord-258014-lzzi4rnz 213 15 between between IN cord-258014-lzzi4rnz 213 16 sample sample NN cord-258014-lzzi4rnz 213 17 groups group NNS cord-258014-lzzi4rnz 213 18 , , , cord-258014-lzzi4rnz 213 19 assessed assess VBN cord-258014-lzzi4rnz 213 20 using use VBG cord-258014-lzzi4rnz 213 21 the the DT cord-258014-lzzi4rnz 213 22 PERMANOVA PERMANOVA NNP cord-258014-lzzi4rnz 213 23 test test NN cord-258014-lzzi4rnz 213 24 , , , cord-258014-lzzi4rnz 213 25 which which WDT cord-258014-lzzi4rnz 213 26 allows allow VBZ cord-258014-lzzi4rnz 213 27 sample sample NN cord-258014-lzzi4rnz 213 28 - - HYPH cord-258014-lzzi4rnz 213 29 sample sample NN cord-258014-lzzi4rnz 213 30 distance distance NN cord-258014-lzzi4rnz 213 31 to to TO cord-258014-lzzi4rnz 213 32 be be VB cord-258014-lzzi4rnz 213 33 applied apply VBN cord-258014-lzzi4rnz 213 34 to to IN cord-258014-lzzi4rnz 213 35 an an DT cord-258014-lzzi4rnz 213 36 Analyses analysis NNS cord-258014-lzzi4rnz 213 37 of of IN cord-258014-lzzi4rnz 213 38 Variance variance NN cord-258014-lzzi4rnz 213 39 ( ( -LRB- cord-258014-lzzi4rnz 213 40 ANOVA)-like anova)-like WRB cord-258014-lzzi4rnz 213 41 framework framework NN cord-258014-lzzi4rnz 213 42 [ [ -LRB- cord-258014-lzzi4rnz 213 43 38 38 CD cord-258014-lzzi4rnz 213 44 ] ] -RRB- cord-258014-lzzi4rnz 213 45 . . . cord-258014-lzzi4rnz 214 1 This this DT cord-258014-lzzi4rnz 214 2 PERMANOVA PERMANOVA NNP cord-258014-lzzi4rnz 214 3 test test NN cord-258014-lzzi4rnz 214 4 is be VBZ cord-258014-lzzi4rnz 214 5 determined determine VBN cord-258014-lzzi4rnz 214 6 through through IN cord-258014-lzzi4rnz 214 7 permutations permutation NNS cord-258014-lzzi4rnz 214 8 and and CC cord-258014-lzzi4rnz 214 9 provides provide VBZ cord-258014-lzzi4rnz 214 10 strength strength NN cord-258014-lzzi4rnz 214 11 and and CC cord-258014-lzzi4rnz 214 12 statistical statistical JJ cord-258014-lzzi4rnz 214 13 significance significance NN cord-258014-lzzi4rnz 214 14 on on IN cord-258014-lzzi4rnz 214 15 sample sample NN cord-258014-lzzi4rnz 214 16 groupings grouping NNS cord-258014-lzzi4rnz 214 17 using use VBG cord-258014-lzzi4rnz 214 18 a a DT cord-258014-lzzi4rnz 214 19 Bray Bray NNP cord-258014-lzzi4rnz 214 20 - - HYPH cord-258014-lzzi4rnz 214 21 Curtis Curtis NNP cord-258014-lzzi4rnz 214 22 distance distance NN cord-258014-lzzi4rnz 214 23 matrix matrix NN cord-258014-lzzi4rnz 214 24 as as IN cord-258014-lzzi4rnz 214 25 the the DT cord-258014-lzzi4rnz 214 26 primary primary JJ cord-258014-lzzi4rnz 214 27 input input NN cord-258014-lzzi4rnz 214 28 . . . cord-258014-lzzi4rnz 215 1 Analyses analysis NNS cord-258014-lzzi4rnz 215 2 of of IN cord-258014-lzzi4rnz 215 3 Variance variance NN cord-258014-lzzi4rnz 215 4 tests test NNS cord-258014-lzzi4rnz 215 5 using use VBG cord-258014-lzzi4rnz 215 6 the the DT cord-258014-lzzi4rnz 215 7 aov aov NNP cord-258014-lzzi4rnz 215 8 function function NN cord-258014-lzzi4rnz 215 9 in in IN cord-258014-lzzi4rnz 215 10 R R NNP cord-258014-lzzi4rnz 215 11 ( ( -LRB- cord-258014-lzzi4rnz 215 12 Team Team NNP cord-258014-lzzi4rnz 215 13 , , , cord-258014-lzzi4rnz 215 14 2008 2008 CD cord-258014-lzzi4rnz 215 15 ) ) -RRB- cord-258014-lzzi4rnz 215 16 must must MD cord-258014-lzzi4rnz 215 17 be be VB cord-258014-lzzi4rnz 215 18 used use VBN cord-258014-lzzi4rnz 215 19 on on IN cord-258014-lzzi4rnz 215 20 the the DT cord-258014-lzzi4rnz 215 21 rarefied rarefied JJ cord-258014-lzzi4rnz 215 22 richness richness NN cord-258014-lzzi4rnz 215 23 ( ( -LRB- cord-258014-lzzi4rnz 215 24 alpha alpha NN cord-258014-lzzi4rnz 215 25 ) ) -RRB- cord-258014-lzzi4rnz 215 26 values value NNS cord-258014-lzzi4rnz 215 27 as as RB cord-258014-lzzi4rnz 215 28 well well RB cord-258014-lzzi4rnz 215 29 as as IN cord-258014-lzzi4rnz 215 30 for for IN cord-258014-lzzi4rnz 215 31 the the DT cord-258014-lzzi4rnz 215 32 Shannon Shannon NNP cord-258014-lzzi4rnz 215 33 diversity diversity NN cord-258014-lzzi4rnz 215 34 values value NNS cord-258014-lzzi4rnz 215 35 , , , cord-258014-lzzi4rnz 215 36 to to TO cord-258014-lzzi4rnz 215 37 find find VB cord-258014-lzzi4rnz 215 38 significant significant JJ cord-258014-lzzi4rnz 215 39 differences difference NNS cord-258014-lzzi4rnz 215 40 related relate VBN cord-258014-lzzi4rnz 215 41 to to IN cord-258014-lzzi4rnz 215 42 a a DT cord-258014-lzzi4rnz 215 43 given give VBN cord-258014-lzzi4rnz 215 44 category category NN cord-258014-lzzi4rnz 215 45 . . . cord-258014-lzzi4rnz 216 1 p p NN cord-258014-lzzi4rnz 216 2 - - : cord-258014-lzzi4rnz 216 3 Values value NNS cord-258014-lzzi4rnz 216 4 should should MD cord-258014-lzzi4rnz 216 5 be be VB cord-258014-lzzi4rnz 216 6 considered consider VBN cord-258014-lzzi4rnz 216 7 using use VBG cord-258014-lzzi4rnz 216 8 an an DT cord-258014-lzzi4rnz 216 9 FDR FDR NNP cord-258014-lzzi4rnz 216 10 of of IN cord-258014-lzzi4rnz 216 11 0.05 0.05 CD cord-258014-lzzi4rnz 216 12 to to TO cord-258014-lzzi4rnz 216 13 be be VB cord-258014-lzzi4rnz 216 14 considered consider VBN cord-258014-lzzi4rnz 216 15 significant significant JJ cord-258014-lzzi4rnz 216 16 . . . cord-258014-lzzi4rnz 217 1 3 3 LS cord-258014-lzzi4rnz 217 2 . . . cord-258014-lzzi4rnz 218 1 The the DT cord-258014-lzzi4rnz 218 2 differential differential JJ cord-258014-lzzi4rnz 218 3 abundance abundance NN cord-258014-lzzi4rnz 218 4 test test NN cord-258014-lzzi4rnz 218 5 for for IN cord-258014-lzzi4rnz 218 6 OTUs otu NNS cord-258014-lzzi4rnz 218 7 , , , cord-258014-lzzi4rnz 218 8 to to TO cord-258014-lzzi4rnz 218 9 identify identify VB cord-258014-lzzi4rnz 218 10 possible possible JJ cord-258014-lzzi4rnz 218 11 signatures signature NNS cord-258014-lzzi4rnz 218 12 significantly significantly RB cord-258014-lzzi4rnz 218 13 associated associate VBN cord-258014-lzzi4rnz 218 14 with with IN cord-258014-lzzi4rnz 218 15 the the DT cord-258014-lzzi4rnz 218 16 metadata metadata NN cord-258014-lzzi4rnz 218 17 categories category NNS cord-258014-lzzi4rnz 218 18 ( ( -LRB- cord-258014-lzzi4rnz 218 19 biomarkers biomarker NNS cord-258014-lzzi4rnz 218 20 ) ) -RRB- cord-258014-lzzi4rnz 218 21 , , , cord-258014-lzzi4rnz 218 22 can can MD cord-258014-lzzi4rnz 218 23 be be VB cord-258014-lzzi4rnz 218 24 completed complete VBN cord-258014-lzzi4rnz 218 25 with with IN cord-258014-lzzi4rnz 218 26 non non JJ cord-258014-lzzi4rnz 218 27 - - JJ cord-258014-lzzi4rnz 218 28 parametric parametric JJ cord-258014-lzzi4rnz 218 29 Wilcoxon Wilcoxon NNP cord-258014-lzzi4rnz 218 30 rank rank NN cord-258014-lzzi4rnz 218 31 sum sum NN cord-258014-lzzi4rnz 218 32 tests test NNS cord-258014-lzzi4rnz 218 33 that that WDT cord-258014-lzzi4rnz 218 34 have have VBP cord-258014-lzzi4rnz 218 35 lower low JJR cord-258014-lzzi4rnz 218 36 false false JJ cord-258014-lzzi4rnz 218 37 positive positive JJ cord-258014-lzzi4rnz 218 38 rates rate NNS cord-258014-lzzi4rnz 218 39 and and CC cord-258014-lzzi4rnz 218 40 are be VBP cord-258014-lzzi4rnz 218 41 more more RBR cord-258014-lzzi4rnz 218 42 robust robust JJ cord-258014-lzzi4rnz 218 43 to to IN cord-258014-lzzi4rnz 218 44 outliers outlier NNS cord-258014-lzzi4rnz 218 45 , , , cord-258014-lzzi4rnz 218 46 without without IN cord-258014-lzzi4rnz 218 47 needing need VBG cord-258014-lzzi4rnz 218 48 a a DT cord-258014-lzzi4rnz 218 49 normal normal JJ cord-258014-lzzi4rnz 218 50 distribution distribution NN cord-258014-lzzi4rnz 218 51 assumption assumption NN cord-258014-lzzi4rnz 218 52 . . . cord-258014-lzzi4rnz 219 1 Biomarker Biomarker NNP cord-258014-lzzi4rnz 219 2 analyses analysis NNS cord-258014-lzzi4rnz 219 3 can can MD cord-258014-lzzi4rnz 219 4 be be VB cord-258014-lzzi4rnz 219 5 identified identify VBN cord-258014-lzzi4rnz 219 6 also also RB cord-258014-lzzi4rnz 219 7 with with IN cord-258014-lzzi4rnz 219 8 LEFSE LEFSE NNP cord-258014-lzzi4rnz 219 9 [ [ -LRB- cord-258014-lzzi4rnz 219 10 34 34 CD cord-258014-lzzi4rnz 219 11 ] ] -RRB- cord-258014-lzzi4rnz 219 12 . . . cord-258014-lzzi4rnz 220 1 Quantify quantify VB cord-258014-lzzi4rnz 220 2 each each DT cord-258014-lzzi4rnz 220 3 peak peak NN cord-258014-lzzi4rnz 220 4 using use VBG cord-258014-lzzi4rnz 220 5 the the DT cord-258014-lzzi4rnz 220 6 maximum maximum JJ cord-258014-lzzi4rnz 220 7 peak peak NN cord-258014-lzzi4rnz 220 8 intensity intensity NN cord-258014-lzzi4rnz 220 9 value value NN cord-258014-lzzi4rnz 220 10 and and CC cord-258014-lzzi4rnz 220 11 organize organize VB cord-258014-lzzi4rnz 220 12 data datum NNS cord-258014-lzzi4rnz 220 13 as as IN cord-258014-lzzi4rnz 220 14 a a DT cord-258014-lzzi4rnz 220 15 " " `` cord-258014-lzzi4rnz 220 16 peak peak JJ cord-258014-lzzi4rnz 220 17 intensity intensity NN cord-258014-lzzi4rnz 220 18 table table NN cord-258014-lzzi4rnz 220 19 " " '' cord-258014-lzzi4rnz 220 20 using use VBG cord-258014-lzzi4rnz 220 21 MS MS NNP cord-258014-lzzi4rnz 220 22 Excel Excel NNP cord-258014-lzzi4rnz 220 23 or or CC cord-258014-lzzi4rnz 220 24 similar similar JJ cord-258014-lzzi4rnz 220 25 software software NN cord-258014-lzzi4rnz 220 26 ( ( -LRB- cord-258014-lzzi4rnz 220 27 see see VB cord-258014-lzzi4rnz 220 28 Note Note NNP cord-258014-lzzi4rnz 220 29 12 12 CD cord-258014-lzzi4rnz 220 30 ) ) -RRB- cord-258014-lzzi4rnz 220 31 . . . cord-258014-lzzi4rnz 221 1 The the DT cord-258014-lzzi4rnz 221 2 table table NN cord-258014-lzzi4rnz 221 3 must must MD cord-258014-lzzi4rnz 221 4 include include VB cord-258014-lzzi4rnz 221 5 metabolite metabolite JJ cord-258014-lzzi4rnz 221 6 identities identity NNS cord-258014-lzzi4rnz 221 7 and and CC cord-258014-lzzi4rnz 221 8 peak peak NN cord-258014-lzzi4rnz 221 9 intensity intensity NN cord-258014-lzzi4rnz 221 10 values value NNS cord-258014-lzzi4rnz 221 11 and and CC cord-258014-lzzi4rnz 221 12 saved save VBN cord-258014-lzzi4rnz 221 13 in in IN cord-258014-lzzi4rnz 221 14 Comma Comma NNP cord-258014-lzzi4rnz 221 15 Separated separate VBN cord-258014-lzzi4rnz 221 16 Values value NNS cord-258014-lzzi4rnz 221 17 ( ( -LRB- cord-258014-lzzi4rnz 221 18 .csv .csv NFP cord-258014-lzzi4rnz 221 19 ) ) -RRB- cord-258014-lzzi4rnz 221 20 or or CC cord-258014-lzzi4rnz 221 21 Tab Tab NNP cord-258014-lzzi4rnz 221 22 Delimited Delimited NNP cord-258014-lzzi4rnz 221 23 Text Text NNP cord-258014-lzzi4rnz 221 24 ( ( -LRB- cord-258014-lzzi4rnz 221 25 .txt .txt NFP cord-258014-lzzi4rnz 221 26 ) ) -RRB- cord-258014-lzzi4rnz 221 27 . . . cord-258014-lzzi4rnz 222 1 Perform perform VB cord-258014-lzzi4rnz 222 2 statistical statistical JJ cord-258014-lzzi4rnz 222 3 analyses analysis NNS cord-258014-lzzi4rnz 222 4 using use VBG cord-258014-lzzi4rnz 222 5 free free JJ cord-258014-lzzi4rnz 222 6 online online JJ cord-258014-lzzi4rnz 222 7 tool tool NN cord-258014-lzzi4rnz 223 1 MetaboAnalyst.ca metaboanalyst.ca JJ cord-258014-lzzi4rnz 223 2 [ [ -LRB- cord-258014-lzzi4rnz 223 3 39 39 CD cord-258014-lzzi4rnz 223 4 , , , cord-258014-lzzi4rnz 223 5 40 40 CD cord-258014-lzzi4rnz 223 6 ] ] -RRB- cord-258014-lzzi4rnz 224 1 ( ( -LRB- cord-258014-lzzi4rnz 224 2 see see VB cord-258014-lzzi4rnz 224 3 Note Note NNP cord-258014-lzzi4rnz 224 4 13 13 CD cord-258014-lzzi4rnz 224 5 ) ) -RRB- cord-258014-lzzi4rnz 224 6 . . . cord-258014-lzzi4rnz 225 1 2 2 LS cord-258014-lzzi4rnz 225 2 . . . cord-258014-lzzi4rnz 226 1 Open open JJ cord-258014-lzzi4rnz 226 2 MetaboAnalyst.ca metaboanalyst.ca NN cord-258014-lzzi4rnz 226 3 , , , cord-258014-lzzi4rnz 226 4 choose choose VB cord-258014-lzzi4rnz 226 5 " " `` cord-258014-lzzi4rnz 226 6 Statistical Statistical NNP cord-258014-lzzi4rnz 226 7 Analysis Analysis NNP cord-258014-lzzi4rnz 226 8 " " '' cord-258014-lzzi4rnz 226 9 , , , cord-258014-lzzi4rnz 226 10 upload upload VB cord-258014-lzzi4rnz 226 11 the the DT cord-258014-lzzi4rnz 226 12 " " `` cord-258014-lzzi4rnz 226 13 peak peak JJ cord-258014-lzzi4rnz 226 14 intensity intensity NN cord-258014-lzzi4rnz 226 15 table table NN cord-258014-lzzi4rnz 226 16 " " '' cord-258014-lzzi4rnz 226 17 and and CC cord-258014-lzzi4rnz 226 18 submit submit VB cord-258014-lzzi4rnz 226 19 . . . cord-258014-lzzi4rnz 227 1 If if IN cord-258014-lzzi4rnz 227 2 the the DT cord-258014-lzzi4rnz 227 3 table table NN cord-258014-lzzi4rnz 227 4 was be VBD cord-258014-lzzi4rnz 227 5 organized organize VBN cord-258014-lzzi4rnz 227 6 correctly correctly RB cord-258014-lzzi4rnz 227 7 and and CC cord-258014-lzzi4rnz 227 8 saved save VBN cord-258014-lzzi4rnz 227 9 in in IN cord-258014-lzzi4rnz 227 10 the the DT cord-258014-lzzi4rnz 227 11 required require VBN cord-258014-lzzi4rnz 227 12 format format NN cord-258014-lzzi4rnz 227 13 , , , cord-258014-lzzi4rnz 227 14 the the DT cord-258014-lzzi4rnz 227 15 " " `` cord-258014-lzzi4rnz 227 16 Data Data NNP cord-258014-lzzi4rnz 227 17 Integrity integrity NN cord-258014-lzzi4rnz 227 18 Check check NN cord-258014-lzzi4rnz 227 19 " " '' cord-258014-lzzi4rnz 227 20 will will MD cord-258014-lzzi4rnz 227 21 pass pass VB cord-258014-lzzi4rnz 227 22 the the DT cord-258014-lzzi4rnz 227 23 information information NN cord-258014-lzzi4rnz 227 24 for for IN cord-258014-lzzi4rnz 227 25 further further JJ cord-258014-lzzi4rnz 227 26 analysis analysis NN cord-258014-lzzi4rnz 227 27 . . . cord-258014-lzzi4rnz 228 1 Click click VB cord-258014-lzzi4rnz 228 2 the the DT cord-258014-lzzi4rnz 228 3 " " `` cord-258014-lzzi4rnz 228 4 Skip skip VB cord-258014-lzzi4rnz 228 5 " " '' cord-258014-lzzi4rnz 228 6 button button NN cord-258014-lzzi4rnz 228 7 to to TO cord-258014-lzzi4rnz 228 8 accept accept VB cord-258014-lzzi4rnz 228 9 the the DT cord-258014-lzzi4rnz 228 10 default default NN cord-258014-lzzi4rnz 228 11 practice practice NN cord-258014-lzzi4rnz 228 12 ( ( -LRB- cord-258014-lzzi4rnz 228 13 see see VB cord-258014-lzzi4rnz 228 14 Note Note NNP cord-258014-lzzi4rnz 228 15 14 14 CD cord-258014-lzzi4rnz 228 16 ) ) -RRB- cord-258014-lzzi4rnz 228 17 . . . cord-258014-lzzi4rnz 229 1 In in IN cord-258014-lzzi4rnz 229 2 " " `` cord-258014-lzzi4rnz 229 3 Data data NN cord-258014-lzzi4rnz 229 4 Filtering filtering NN cord-258014-lzzi4rnz 229 5 " " '' cord-258014-lzzi4rnz 229 6 click click NN cord-258014-lzzi4rnz 229 7 " " '' cord-258014-lzzi4rnz 229 8 None none NN cord-258014-lzzi4rnz 229 9 " " '' cord-258014-lzzi4rnz 229 10 . . . cord-258014-lzzi4rnz 230 1 4 4 LS cord-258014-lzzi4rnz 230 2 . . . cord-258014-lzzi4rnz 231 1 Perform perform VB cord-258014-lzzi4rnz 231 2 data datum NNS cord-258014-lzzi4rnz 231 3 normalization normalization NN cord-258014-lzzi4rnz 231 4 to to TO cord-258014-lzzi4rnz 231 5 remove remove VB cord-258014-lzzi4rnz 231 6 unwanted unwanted JJ cord-258014-lzzi4rnz 231 7 variations variation NNS cord-258014-lzzi4rnz 231 8 between between IN cord-258014-lzzi4rnz 231 9 the the DT cord-258014-lzzi4rnz 231 10 samples sample NNS cord-258014-lzzi4rnz 231 11 . . . cord-258014-lzzi4rnz 232 1 First first RB cord-258014-lzzi4rnz 232 2 , , , cord-258014-lzzi4rnz 232 3 perform perform VB cord-258014-lzzi4rnz 232 4 " " `` cord-258014-lzzi4rnz 232 5 sample sample NN cord-258014-lzzi4rnz 232 6 - - HYPH cord-258014-lzzi4rnz 232 7 specific specific JJ cord-258014-lzzi4rnz 232 8 normalization normalization NN cord-258014-lzzi4rnz 232 9 . . . cord-258014-lzzi4rnz 233 1 Click click VB cord-258014-lzzi4rnz 233 2 " " `` cord-258014-lzzi4rnz 233 3 Proceed proceed VB cord-258014-lzzi4rnz 233 4 " " '' cord-258014-lzzi4rnz 233 5 ( ( -LRB- cord-258014-lzzi4rnz 233 6 see see VB cord-258014-lzzi4rnz 233 7 Note Note NNP cord-258014-lzzi4rnz 233 8 15 15 CD cord-258014-lzzi4rnz 233 9 ) ) -RRB- cord-258014-lzzi4rnz 233 10 . . . cord-258014-lzzi4rnz 234 1 For for IN cord-258014-lzzi4rnz 234 2 two two CD cord-258014-lzzi4rnz 234 3 groups group NNS cord-258014-lzzi4rnz 234 4 ( ( -LRB- cord-258014-lzzi4rnz 234 5 control control NN cord-258014-lzzi4rnz 234 6 and and CC cord-258014-lzzi4rnz 234 7 experimental experimental JJ cord-258014-lzzi4rnz 234 8 ) ) -RRB- cord-258014-lzzi4rnz 234 9 perform perform VB cord-258014-lzzi4rnz 234 10 univariate univariate JJ cord-258014-lzzi4rnz 234 11 analysis analysis NN cord-258014-lzzi4rnz 234 12 ( ( -LRB- cord-258014-lzzi4rnz 234 13 fold fold VB cord-258014-lzzi4rnz 234 14 change change NN cord-258014-lzzi4rnz 234 15 analysis analysis NN cord-258014-lzzi4rnz 234 16 , , , cord-258014-lzzi4rnz 234 17 T t NN cord-258014-lzzi4rnz 234 18 - - HYPH cord-258014-lzzi4rnz 234 19 tests test NNS cord-258014-lzzi4rnz 234 20 , , , cord-258014-lzzi4rnz 234 21 volcano volcano NN cord-258014-lzzi4rnz 234 22 plot plot NN cord-258014-lzzi4rnz 234 23 , , , cord-258014-lzzi4rnz 234 24 correlation correlation NN cord-258014-lzzi4rnz 234 25 analysis analysis NN cord-258014-lzzi4rnz 234 26 ) ) -RRB- cord-258014-lzzi4rnz 234 27 . . . cord-258014-lzzi4rnz 235 1 6 6 LS cord-258014-lzzi4rnz 235 2 . . . cord-258014-lzzi4rnz 236 1 For for IN cord-258014-lzzi4rnz 236 2 more more JJR cord-258014-lzzi4rnz 236 3 groups group NNS cord-258014-lzzi4rnz 236 4 , , , cord-258014-lzzi4rnz 236 5 perform perform VB cord-258014-lzzi4rnz 236 6 chemometrics chemometric NNS cord-258014-lzzi4rnz 236 7 analysis analysis NN cord-258014-lzzi4rnz 237 1 ( ( -LRB- cord-258014-lzzi4rnz 237 2 Principal Principal NNP cord-258014-lzzi4rnz 237 3 Component Component NNP cord-258014-lzzi4rnz 237 4 Analysis Analysis NNP cord-258014-lzzi4rnz 237 5 ( ( -LRB- cord-258014-lzzi4rnz 237 6 PCA PCA NNP cord-258014-lzzi4rnz 237 7 ) ) -RRB- cord-258014-lzzi4rnz 237 8 , , . cord-258014-lzzi4rnz 238 1 Partial Partial NNP cord-258014-lzzi4rnz 238 2 Least Least NNP cord-258014-lzzi4rnz 238 3 Squares Squares NNPS cord-258014-lzzi4rnz 238 4 - - HYPH cord-258014-lzzi4rnz 238 5 Discriminant Discriminant NNP cord-258014-lzzi4rnz 238 6 Analysis Analysis NNP cord-258014-lzzi4rnz 238 7 ( ( -LRB- cord-258014-lzzi4rnz 238 8 PLS PLS NNP cord-258014-lzzi4rnz 238 9 - - HYPH cord-258014-lzzi4rnz 238 10 DA DA NNP cord-258014-lzzi4rnz 238 11 ) ) -RRB- cord-258014-lzzi4rnz 238 12 , , , cord-258014-lzzi4rnz 238 13 feature feature VBP cord-258014-lzzi4rnz 238 14 identification identification NN cord-258014-lzzi4rnz 238 15 and and CC cord-258014-lzzi4rnz 238 16 cluster cluster NN cord-258014-lzzi4rnz 238 17 analysis analysis NN cord-258014-lzzi4rnz 238 18 . . . cord-258014-lzzi4rnz 239 1 Detect detect VB cord-258014-lzzi4rnz 239 2 outliers outlier NNS cord-258014-lzzi4rnz 239 3 to to TO cord-258014-lzzi4rnz 239 4 improve improve VB cord-258014-lzzi4rnz 239 5 downstream downstream JJ cord-258014-lzzi4rnz 239 6 results result NNS cord-258014-lzzi4rnz 239 7 ( ( -LRB- cord-258014-lzzi4rnz 239 8 see see VB cord-258014-lzzi4rnz 239 9 Note Note NNP cord-258014-lzzi4rnz 239 10 16 16 CD cord-258014-lzzi4rnz 239 11 ) ) -RRB- cord-258014-lzzi4rnz 239 12 . . . cord-258014-lzzi4rnz 240 1 The the DT cord-258014-lzzi4rnz 240 2 integration integration NN cord-258014-lzzi4rnz 240 3 of of IN cord-258014-lzzi4rnz 240 4 metagenomics metagenomic NNS cord-258014-lzzi4rnz 240 5 and and CC cord-258014-lzzi4rnz 240 6 metabolomics metabolomic NNS cord-258014-lzzi4rnz 240 7 data datum NNS cord-258014-lzzi4rnz 240 8 could could MD cord-258014-lzzi4rnz 240 9 be be VB cord-258014-lzzi4rnz 240 10 performed perform VBN cord-258014-lzzi4rnz 240 11 using use VBG cord-258014-lzzi4rnz 240 12 Model model NN cord-258014-lzzi4rnz 240 13 - - HYPH cord-258014-lzzi4rnz 240 14 based base VBN cord-258014-lzzi4rnz 240 15 Integration Integration NNP cord-258014-lzzi4rnz 240 16 of of IN cord-258014-lzzi4rnz 240 17 Metabolite Metabolite NNP cord-258014-lzzi4rnz 240 18 Observations Observations NNPS cord-258014-lzzi4rnz 240 19 and and CC cord-258014-lzzi4rnz 240 20 Species Species NNPS cord-258014-lzzi4rnz 240 21 Abundances Abundances NNPS cord-258014-lzzi4rnz 240 22 2 2 CD cord-258014-lzzi4rnz 240 23 ( ( -LRB- cord-258014-lzzi4rnz 240 24 MIMOSA2 MIMOSA2 NNP cord-258014-lzzi4rnz 240 25 ) ) -RRB- cord-258014-lzzi4rnz 240 26 freely freely RB cord-258014-lzzi4rnz 240 27 available available JJ cord-258014-lzzi4rnz 240 28 at at IN cord-258014-lzzi4rnz 240 29 borensteinlab.com/software_MIMOSA2.html borensteinlab.com/software_mimosa2.html NN cord-258014-lzzi4rnz 240 30 [ [ -LRB- cord-258014-lzzi4rnz 240 31 41 41 CD cord-258014-lzzi4rnz 240 32 , , , cord-258014-lzzi4rnz 240 33 42 42 CD cord-258014-lzzi4rnz 240 34 ] ] -RRB- cord-258014-lzzi4rnz 240 35 . . . cord-258014-lzzi4rnz 241 1 MIMOSA2 MIMOSA2 NNP cord-258014-lzzi4rnz 242 1 summarizes summarize VBZ cord-258014-lzzi4rnz 242 2 paired pair VBN cord-258014-lzzi4rnz 242 3 microbiome microbiome NN cord-258014-lzzi4rnz 242 4 - - HYPH cord-258014-lzzi4rnz 242 5 metabolome metabolome NN cord-258014-lzzi4rnz 242 6 datasets dataset NNS cord-258014-lzzi4rnz 242 7 to to TO cord-258014-lzzi4rnz 242 8 support support VB cord-258014-lzzi4rnz 242 9 mechanistic mechanistic JJ cord-258014-lzzi4rnz 242 10 interpretation interpretation NN cord-258014-lzzi4rnz 242 11 and and CC cord-258014-lzzi4rnz 242 12 hypothesis hypothesis NN cord-258014-lzzi4rnz 242 13 generation generation NN cord-258014-lzzi4rnz 242 14 . . . cord-258014-lzzi4rnz 243 1 MIMOSA2 MIMOSA2 NNP cord-258014-lzzi4rnz 243 2 also also RB cord-258014-lzzi4rnz 243 3 characterizes characterize VBZ cord-258014-lzzi4rnz 243 4 the the DT cord-258014-lzzi4rnz 243 5 relative relative JJ cord-258014-lzzi4rnz 243 6 capacity capacity NN cord-258014-lzzi4rnz 243 7 of of IN cord-258014-lzzi4rnz 243 8 community community NN cord-258014-lzzi4rnz 243 9 members member NNS cord-258014-lzzi4rnz 243 10 to to TO cord-258014-lzzi4rnz 243 11 produce produce VB cord-258014-lzzi4rnz 243 12 or or CC cord-258014-lzzi4rnz 243 13 consume consume VB cord-258014-lzzi4rnz 243 14 metabolites metabolite NNS cord-258014-lzzi4rnz 243 15 based base VBN cord-258014-lzzi4rnz 243 16 on on IN cord-258014-lzzi4rnz 243 17 a a DT cord-258014-lzzi4rnz 243 18 priori priori FW cord-258014-lzzi4rnz 243 19 metabolic metabolic JJ cord-258014-lzzi4rnz 243 20 information information NN cord-258014-lzzi4rnz 243 21 of of IN cord-258014-lzzi4rnz 243 22 the the DT cord-258014-lzzi4rnz 243 23 activity activity NN cord-258014-lzzi4rnz 243 24 of of IN cord-258014-lzzi4rnz 243 25 metabolic metabolic JJ cord-258014-lzzi4rnz 243 26 enzymes enzyme NNS cord-258014-lzzi4rnz 243 27 for for IN cord-258014-lzzi4rnz 243 28 each each DT cord-258014-lzzi4rnz 243 29 species specie NNS cord-258014-lzzi4rnz 243 30 from from IN cord-258014-lzzi4rnz 243 31 the the DT cord-258014-lzzi4rnz 243 32 KEGG KEGG NNP cord-258014-lzzi4rnz 243 33 database database NN cord-258014-lzzi4rnz 243 34 , , , cord-258014-lzzi4rnz 243 35 describes describe VBZ cord-258014-lzzi4rnz 243 36 how how WRB cord-258014-lzzi4rnz 243 37 well well RB cord-258014-lzzi4rnz 243 38 each each DT cord-258014-lzzi4rnz 243 39 metabolite metabolite NN cord-258014-lzzi4rnz 243 40 can can MD cord-258014-lzzi4rnz 243 41 be be VB cord-258014-lzzi4rnz 243 42 predicted predict VBN cord-258014-lzzi4rnz 243 43 by by IN cord-258014-lzzi4rnz 243 44 metabolic metabolic JJ cord-258014-lzzi4rnz 243 45 potential potential NN cord-258014-lzzi4rnz 243 46 , , , cord-258014-lzzi4rnz 243 47 and and CC cord-258014-lzzi4rnz 243 48 estimates estimate VBZ cord-258014-lzzi4rnz 243 49 how how WRB cord-258014-lzzi4rnz 243 50 much much JJ cord-258014-lzzi4rnz 243 51 each each DT cord-258014-lzzi4rnz 243 52 taxon taxon NN cord-258014-lzzi4rnz 243 53 can can MD cord-258014-lzzi4rnz 243 54 explain explain VB cord-258014-lzzi4rnz 243 55 each each DT cord-258014-lzzi4rnz 243 56 metabolite metabolite NN cord-258014-lzzi4rnz 243 57 . . . cord-258014-lzzi4rnz 244 1 Prepare prepare VB cord-258014-lzzi4rnz 244 2 the the DT cord-258014-lzzi4rnz 244 3 output output NN cord-258014-lzzi4rnz 244 4 data data NN cord-258014-lzzi4rnz 244 5 files file NNS cord-258014-lzzi4rnz 244 6 and and CC cord-258014-lzzi4rnz 244 7 save save VB cord-258014-lzzi4rnz 244 8 in in IN cord-258014-lzzi4rnz 244 9 txt txt . cord-258014-lzzi4rnz 244 10 format format NN cord-258014-lzzi4rnz 244 11 for for IN cord-258014-lzzi4rnz 244 12 each each DT cord-258014-lzzi4rnz 244 13 sample sample NN cord-258014-lzzi4rnz 244 14 in in IN cord-258014-lzzi4rnz 244 15 either either DT cord-258014-lzzi4rnz 244 16 control control NN cord-258014-lzzi4rnz 244 17 or or CC cord-258014-lzzi4rnz 244 18 experimental experimental JJ cord-258014-lzzi4rnz 244 19 groups group NNS cord-258014-lzzi4rnz 244 20 separately separately RB cord-258014-lzzi4rnz 244 21 . . . cord-258014-lzzi4rnz 245 1 For for IN cord-258014-lzzi4rnz 245 2 " " `` cord-258014-lzzi4rnz 245 3 microbiome microbiome NN cord-258014-lzzi4rnz 245 4 " " '' cord-258014-lzzi4rnz 245 5 : : : cord-258014-lzzi4rnz 245 6 From from IN cord-258014-lzzi4rnz 245 7 QIITA QIITA NNP cord-258014-lzzi4rnz 245 8 or or CC cord-258014-lzzi4rnz 245 9 QIIME QIIME NNP cord-258014-lzzi4rnz 245 10 , , , cord-258014-lzzi4rnz 245 11 export export VB cord-258014-lzzi4rnz 245 12 the the DT cord-258014-lzzi4rnz 245 13 species species NN cord-258014-lzzi4rnz 245 14 table table NN cord-258014-lzzi4rnz 245 15 ( ( -LRB- cord-258014-lzzi4rnz 245 16 BIOM BIOM NNP cord-258014-lzzi4rnz 245 17 ) ) -RRB- cord-258014-lzzi4rnz 245 18 with with IN cord-258014-lzzi4rnz 245 19 Greengenes Greengenes NNP cord-258014-lzzi4rnz 245 20 ID ID NNP cord-258014-lzzi4rnz 245 21 as as IN cord-258014-lzzi4rnz 245 22 OTU otu NN cord-258014-lzzi4rnz 245 23 identifiers identifier NNS cord-258014-lzzi4rnz 245 24 [ [ -LRB- cord-258014-lzzi4rnz 245 25 43 43 CD cord-258014-lzzi4rnz 245 26 ] ] -RRB- cord-258014-lzzi4rnz 245 27 . . . cord-258014-lzzi4rnz 246 1 This this DT cord-258014-lzzi4rnz 246 2 will will MD cord-258014-lzzi4rnz 246 3 allow allow VB cord-258014-lzzi4rnz 246 4 for for IN cord-258014-lzzi4rnz 246 5 PICRUST PICRUST NNP cord-258014-lzzi4rnz 246 6 [ [ -LRB- cord-258014-lzzi4rnz 246 7 44 44 CD cord-258014-lzzi4rnz 246 8 ] ] -RRB- cord-258014-lzzi4rnz 246 9 to to TO cord-258014-lzzi4rnz 246 10 use use VB cord-258014-lzzi4rnz 246 11 the the DT cord-258014-lzzi4rnz 246 12 OTU OTU NNP cord-258014-lzzi4rnz 246 13 table table NN cord-258014-lzzi4rnz 246 14 and and CC cord-258014-lzzi4rnz 246 15 result result VB cord-258014-lzzi4rnz 246 16 in in IN cord-258014-lzzi4rnz 246 17 a a DT cord-258014-lzzi4rnz 246 18 functional functional JJ cord-258014-lzzi4rnz 246 19 - - HYPH cord-258014-lzzi4rnz 246 20 gene gene NN cord-258014-lzzi4rnz 246 21 - - HYPH cord-258014-lzzi4rnz 246 22 count count NN cord-258014-lzzi4rnz 246 23 matrix matrix NN cord-258014-lzzi4rnz 246 24 , , , cord-258014-lzzi4rnz 246 25 telling tell VBG cord-258014-lzzi4rnz 246 26 the the DT cord-258014-lzzi4rnz 246 27 count count NN cord-258014-lzzi4rnz 246 28 of of IN cord-258014-lzzi4rnz 246 29 each each DT cord-258014-lzzi4rnz 246 30 functional functional JJ cord-258014-lzzi4rnz 246 31 - - HYPH cord-258014-lzzi4rnz 246 32 gene gene NN cord-258014-lzzi4rnz 246 33 in in IN cord-258014-lzzi4rnz 246 34 each each DT cord-258014-lzzi4rnz 246 35 of of IN cord-258014-lzzi4rnz 246 36 the the DT cord-258014-lzzi4rnz 246 37 samples sample NNS cord-258014-lzzi4rnz 246 38 surveyed survey VBN cord-258014-lzzi4rnz 246 39 , , , cord-258014-lzzi4rnz 246 40 based base VBN cord-258014-lzzi4rnz 246 41 on on IN cord-258014-lzzi4rnz 246 42 the the DT cord-258014-lzzi4rnz 246 43 reference reference NN cord-258014-lzzi4rnz 246 44 genome genome NN cord-258014-lzzi4rnz 246 45 . . . cord-258014-lzzi4rnz 247 1 For for IN cord-258014-lzzi4rnz 247 2 " " `` cord-258014-lzzi4rnz 247 3 metabolome"-use metabolome"-use VB cord-258014-lzzi4rnz 247 4 KEGG KEGG NNP cord-258014-lzzi4rnz 247 5 [ [ -LRB- cord-258014-lzzi4rnz 247 6 45 45 CD cord-258014-lzzi4rnz 247 7 ] ] -RRB- cord-258014-lzzi4rnz 247 8 IDs id NNS cord-258014-lzzi4rnz 247 9 for for IN cord-258014-lzzi4rnz 247 10 each each DT cord-258014-lzzi4rnz 247 11 identified identify VBN cord-258014-lzzi4rnz 247 12 metabolite metabolite NN cord-258014-lzzi4rnz 248 1 and and CC cord-258014-lzzi4rnz 248 2 it -PRON- PRP cord-258014-lzzi4rnz 248 3 's be VBZ cord-258014-lzzi4rnz 248 4 identified identify VBN cord-258014-lzzi4rnz 248 5 concentration concentration NN cord-258014-lzzi4rnz 248 6 . . . cord-258014-lzzi4rnz 249 1 Log log VB cord-258014-lzzi4rnz 249 2 in in RP cord-258014-lzzi4rnz 249 3 to to IN cord-258014-lzzi4rnz 249 4 borensteinlab.com/software_MIMOSA2.html borensteinlab.com/software_mimosa2.html NN cord-258014-lzzi4rnz 249 5 . . . cord-258014-lzzi4rnz 250 1 The the DT cord-258014-lzzi4rnz 250 2 following following JJ cord-258014-lzzi4rnz 250 3 options option NNS cord-258014-lzzi4rnz 250 4 will will MD cord-258014-lzzi4rnz 250 5 be be VB cord-258014-lzzi4rnz 250 6 used use VBN cord-258014-lzzi4rnz 250 7 for for IN cord-258014-lzzi4rnz 250 8 the the DT cord-258014-lzzi4rnz 250 9 analysis analysis NN cord-258014-lzzi4rnz 250 10 : : : cord-258014-lzzi4rnz 250 11 4 4 CD cord-258014-lzzi4rnz 250 12 . . . cord-258014-lzzi4rnz 251 1 MIMOSA2 MIMOSA2 NNP cord-258014-lzzi4rnz 251 2 will will MD cord-258014-lzzi4rnz 251 3 calculate calculate VB cord-258014-lzzi4rnz 251 4 CMP CMP NNP cord-258014-lzzi4rnz 251 5 scores score NNS cord-258014-lzzi4rnz 251 6 for for IN cord-258014-lzzi4rnz 251 7 each each DT cord-258014-lzzi4rnz 251 8 metabolite metabolite NN cord-258014-lzzi4rnz 251 9 and and CC cord-258014-lzzi4rnz 251 10 generate generate VB cord-258014-lzzi4rnz 251 11 a a DT cord-258014-lzzi4rnz 251 12 tab tab NN cord-258014-lzzi4rnz 251 13 - - HYPH cord-258014-lzzi4rnz 251 14 delimited delimited JJ cord-258014-lzzi4rnz 251 15 file file NN cord-258014-lzzi4rnz 251 16 for for IN cord-258014-lzzi4rnz 251 17 each each DT cord-258014-lzzi4rnz 251 18 bacterial bacterial JJ cord-258014-lzzi4rnz 251 19 species specie NNS cord-258014-lzzi4rnz 251 20 in in IN cord-258014-lzzi4rnz 251 21 each each DT cord-258014-lzzi4rnz 251 22 condition condition NN cord-258014-lzzi4rnz 251 23 . . . cord-258014-lzzi4rnz 252 1 Additionally additionally RB cord-258014-lzzi4rnz 252 2 , , , cord-258014-lzzi4rnz 252 3 a a DT cord-258014-lzzi4rnz 252 4 contribution contribution NN cord-258014-lzzi4rnz 252 5 table table NN cord-258014-lzzi4rnz 252 6 will will MD cord-258014-lzzi4rnz 252 7 be be VB cord-258014-lzzi4rnz 252 8 generated generate VBN cord-258014-lzzi4rnz 252 9 as as IN cord-258014-lzzi4rnz 252 10 a a DT cord-258014-lzzi4rnz 252 11 tab tab NN cord-258014-lzzi4rnz 252 12 - - HYPH cord-258014-lzzi4rnz 252 13 delimited delimited JJ cord-258014-lzzi4rnz 252 14 file file NN cord-258014-lzzi4rnz 252 15 which which WDT cord-258014-lzzi4rnz 252 16 will will MD cord-258014-lzzi4rnz 252 17 relate relate VB cord-258014-lzzi4rnz 252 18 taxa taxon NNS cord-258014-lzzi4rnz 252 19 , , , cord-258014-lzzi4rnz 252 20 to to IN cord-258014-lzzi4rnz 252 21 KEGG KEGG VBN cord-258014-lzzi4rnz 252 22 metabolic metabolic JJ cord-258014-lzzi4rnz 252 23 pathways pathway NNS cord-258014-lzzi4rnz 252 24 with with IN cord-258014-lzzi4rnz 252 25 its -PRON- PRP$ cord-258014-lzzi4rnz 252 26 associated associated NN cord-258014-lzzi4rnz 252 27 p p NN cord-258014-lzzi4rnz 252 28 - - HYPH cord-258014-lzzi4rnz 252 29 value value NN cord-258014-lzzi4rnz 252 30 , , , cord-258014-lzzi4rnz 252 31 model model NN cord-258014-lzzi4rnz 252 32 slope slope NN cord-258014-lzzi4rnz 252 33 and and CC cord-258014-lzzi4rnz 252 34 VarShare VarShare NNP cord-258014-lzzi4rnz 252 35 ( ( -LRB- cord-258014-lzzi4rnz 252 36 represent represent VBP cord-258014-lzzi4rnz 252 37 the the DT cord-258014-lzzi4rnz 252 38 fraction fraction NN cord-258014-lzzi4rnz 252 39 of of IN cord-258014-lzzi4rnz 252 40 the the DT cord-258014-lzzi4rnz 252 41 variation variation NN cord-258014-lzzi4rnz 252 42 ) ) -RRB- cord-258014-lzzi4rnz 252 43 for for IN cord-258014-lzzi4rnz 252 44 each each DT cord-258014-lzzi4rnz 252 45 metabolite metabolite NN cord-258014-lzzi4rnz 252 46 explained explain VBN cord-258014-lzzi4rnz 252 47 by by IN cord-258014-lzzi4rnz 252 48 the the DT cord-258014-lzzi4rnz 252 49 taxon taxon NN cord-258014-lzzi4rnz 252 50 in in IN cord-258014-lzzi4rnz 252 51 question question NN cord-258014-lzzi4rnz 252 52 . . . cord-258014-lzzi4rnz 253 1 VarShare VarShare NNP cord-258014-lzzi4rnz 253 2 values value NNS cord-258014-lzzi4rnz 253 3 could could MD cord-258014-lzzi4rnz 253 4 be be VB cord-258014-lzzi4rnz 253 5 used use VBN cord-258014-lzzi4rnz 253 6 to to TO cord-258014-lzzi4rnz 253 7 build build VB cord-258014-lzzi4rnz 253 8 heatmaps heatmap NNS cord-258014-lzzi4rnz 253 9 in in IN cord-258014-lzzi4rnz 253 10 R R NNP cord-258014-lzzi4rnz 253 11 , , , cord-258014-lzzi4rnz 253 12 in in IN cord-258014-lzzi4rnz 253 13 the the DT cord-258014-lzzi4rnz 253 14 same same JJ cord-258014-lzzi4rnz 253 15 format format NN cord-258014-lzzi4rnz 253 16 as as IN cord-258014-lzzi4rnz 253 17 those those DT cord-258014-lzzi4rnz 253 18 for for IN cord-258014-lzzi4rnz 253 19 microbial microbial JJ cord-258014-lzzi4rnz 253 20 taxa taxon NNS cord-258014-lzzi4rnz 253 21 . . . cord-258014-lzzi4rnz 254 1 Finally finally RB cord-258014-lzzi4rnz 254 2 , , , cord-258014-lzzi4rnz 254 3 MIMOSA2 MIMOSA2 NNS cord-258014-lzzi4rnz 254 4 will will MD cord-258014-lzzi4rnz 254 5 identify identify VB cord-258014-lzzi4rnz 254 6 microbial microbial JJ cord-258014-lzzi4rnz 254 7 features feature NNS cord-258014-lzzi4rnz 254 8 that that WDT cord-258014-lzzi4rnz 254 9 may may MD cord-258014-lzzi4rnz 254 10 underlie underlie VB cord-258014-lzzi4rnz 254 11 differences difference NNS cord-258014-lzzi4rnz 254 12 in in IN cord-258014-lzzi4rnz 254 13 microbial microbial JJ cord-258014-lzzi4rnz 254 14 metabolite metabolite JJ cord-258014-lzzi4rnz 254 15 concentrations concentration NNS cord-258014-lzzi4rnz 254 16 between between IN cord-258014-lzzi4rnz 254 17 similar similar JJ cord-258014-lzzi4rnz 254 18 communities community NNS cord-258014-lzzi4rnz 254 19 . . . cord-258014-lzzi4rnz 255 1 It -PRON- PRP cord-258014-lzzi4rnz 255 2 answers answer VBZ cord-258014-lzzi4rnz 255 3 questions question NNS cord-258014-lzzi4rnz 255 4 such such JJ cord-258014-lzzi4rnz 255 5 as as IN cord-258014-lzzi4rnz 255 6 : : : cord-258014-lzzi4rnz 255 7 ( ( -LRB- cord-258014-lzzi4rnz 255 8 1 1 LS cord-258014-lzzi4rnz 255 9 ) ) -RRB- cord-258014-lzzi4rnz 255 10 Do do VB cord-258014-lzzi4rnz 255 11 the the DT cord-258014-lzzi4rnz 255 12 metabolites metabolite NNS cord-258014-lzzi4rnz 255 13 in in IN cord-258014-lzzi4rnz 255 14 a a DT cord-258014-lzzi4rnz 255 15 given give VBN cord-258014-lzzi4rnz 255 16 dataset dataset NN cord-258014-lzzi4rnz 255 17 appear appear VBP cord-258014-lzzi4rnz 255 18 to to TO cord-258014-lzzi4rnz 255 19 vary vary VB cord-258014-lzzi4rnz 255 20 depending depend VBG cord-258014-lzzi4rnz 255 21 on on IN cord-258014-lzzi4rnz 255 22 microbiome microbiome NN cord-258014-lzzi4rnz 255 23 composition composition NN cord-258014-lzzi4rnz 255 24 ? ? . cord-258014-lzzi4rnz 256 1 Which which WDT cord-258014-lzzi4rnz 256 2 ones one NNS cord-258014-lzzi4rnz 256 3 ? ? . cord-258014-lzzi4rnz 257 1 ( ( -LRB- cord-258014-lzzi4rnz 257 2 2 2 LS cord-258014-lzzi4rnz 257 3 ) ) -RRB- cord-258014-lzzi4rnz 257 4 Can Can MD cord-258014-lzzi4rnz 257 5 differences difference NNS cord-258014-lzzi4rnz 257 6 in in IN cord-258014-lzzi4rnz 257 7 microbiome microbiome NN cord-258014-lzzi4rnz 257 8 metabolic metabolic JJ cord-258014-lzzi4rnz 257 9 capabilities capability NNS cord-258014-lzzi4rnz 257 10 explain explain VBP cord-258014-lzzi4rnz 257 11 metabolite metabolite NN cord-258014-lzzi4rnz 257 12 variation variation NN cord-258014-lzzi4rnz 257 13 ? ? . cord-258014-lzzi4rnz 258 1 ( ( -LRB- cord-258014-lzzi4rnz 258 2 3 3 LS cord-258014-lzzi4rnz 258 3 ) ) -RRB- cord-258014-lzzi4rnz 259 1 Which which WDT cord-258014-lzzi4rnz 259 2 taxa taxon NNS cord-258014-lzzi4rnz 259 3 , , , cord-258014-lzzi4rnz 259 4 genes gene NNS cord-258014-lzzi4rnz 259 5 , , , cord-258014-lzzi4rnz 259 6 and and CC cord-258014-lzzi4rnz 259 7 reactions reaction NNS cord-258014-lzzi4rnz 259 8 appear appear VBP cord-258014-lzzi4rnz 259 9 to to TO cord-258014-lzzi4rnz 259 10 be be VB cord-258014-lzzi4rnz 259 11 playing play VBG cord-258014-lzzi4rnz 259 12 a a DT cord-258014-lzzi4rnz 259 13 role role NN cord-258014-lzzi4rnz 259 14 in in IN cord-258014-lzzi4rnz 259 15 metabolite metabolite JJ cord-258014-lzzi4rnz 259 16 differences difference NNS cord-258014-lzzi4rnz 259 17 ? ? . cord-258014-lzzi4rnz 260 1 [ [ -LRB- cord-258014-lzzi4rnz 260 2 42 42 CD cord-258014-lzzi4rnz 260 3 ] ] -RRB- cord-258014-lzzi4rnz 260 4 . . . cord-258014-lzzi4rnz 261 1 Methoxyamine Methoxyamine NNP cord-258014-lzzi4rnz 261 2 hydrochloride hydrochloride NN cord-258014-lzzi4rnz 261 3 is be VBZ cord-258014-lzzi4rnz 261 4 very very RB cord-258014-lzzi4rnz 261 5 sensitive sensitive JJ cord-258014-lzzi4rnz 261 6 to to IN cord-258014-lzzi4rnz 261 7 moisture moisture NN cord-258014-lzzi4rnz 261 8 . . . cord-258014-lzzi4rnz 262 1 Storage storage NN cord-258014-lzzi4rnz 262 2 is be VBZ cord-258014-lzzi4rnz 262 3 recommended recommend VBN cord-258014-lzzi4rnz 262 4 under under IN cord-258014-lzzi4rnz 262 5 the the DT cord-258014-lzzi4rnz 262 6 vacuum vacuum NN cord-258014-lzzi4rnz 262 7 at at IN cord-258014-lzzi4rnz 262 8 RT RT NNP cord-258014-lzzi4rnz 262 9 . . . cord-258014-lzzi4rnz 263 1 Glass glass NN cord-258014-lzzi4rnz 263 2 inserts insert NNS cord-258014-lzzi4rnz 263 3 decrease decrease VBP cord-258014-lzzi4rnz 263 4 the the DT cord-258014-lzzi4rnz 263 5 surface surface NN cord-258014-lzzi4rnz 263 6 area area NN cord-258014-lzzi4rnz 263 7 inside inside IN cord-258014-lzzi4rnz 263 8 the the DT cord-258014-lzzi4rnz 263 9 vial vial NN cord-258014-lzzi4rnz 263 10 allowing allow VBG cord-258014-lzzi4rnz 263 11 to to TO cord-258014-lzzi4rnz 263 12 achieve achieve VB cord-258014-lzzi4rnz 263 13 maximum maximum JJ cord-258014-lzzi4rnz 263 14 sample sample NN cord-258014-lzzi4rnz 263 15 recovery recovery NN cord-258014-lzzi4rnz 263 16 and and CC cord-258014-lzzi4rnz 263 17 easier easy JJR cord-258014-lzzi4rnz 263 18 sample sample NN cord-258014-lzzi4rnz 263 19 removal removal NN cord-258014-lzzi4rnz 263 20 . . . cord-258014-lzzi4rnz 264 1 De De NNP cord-258014-lzzi4rnz 264 2 - - NN cord-258014-lzzi4rnz 264 3 assemble assemble VB cord-258014-lzzi4rnz 264 4 the the DT cord-258014-lzzi4rnz 264 5 screw screw NN cord-258014-lzzi4rnz 264 6 cap cap NN cord-258014-lzzi4rnz 264 7 and and CC cord-258014-lzzi4rnz 264 8 remove remove VB cord-258014-lzzi4rnz 264 9 silicone silicone NN cord-258014-lzzi4rnz 264 10 septum septum NN cord-258014-lzzi4rnz 264 11 . . . cord-258014-lzzi4rnz 265 1 Perforate perforate VB cord-258014-lzzi4rnz 265 2 it -PRON- PRP cord-258014-lzzi4rnz 265 3 using use VBG cord-258014-lzzi4rnz 265 4 scissors scissor NNS cord-258014-lzzi4rnz 265 5 and and CC cord-258014-lzzi4rnz 265 6 mount mount VB cord-258014-lzzi4rnz 265 7 the the DT cord-258014-lzzi4rnz 265 8 vials vial NNS cord-258014-lzzi4rnz 265 9 onto onto IN cord-258014-lzzi4rnz 265 10 a a DT cord-258014-lzzi4rnz 265 11 rotary rotary JJ cord-258014-lzzi4rnz 265 12 vacuum vacuum NN cord-258014-lzzi4rnz 265 13 evaporator evaporator NN cord-258014-lzzi4rnz 265 14 . . . cord-258014-lzzi4rnz 266 1 This this DT cord-258014-lzzi4rnz 266 2 step step NN cord-258014-lzzi4rnz 266 3 is be VBZ cord-258014-lzzi4rnz 266 4 required require VBN cord-258014-lzzi4rnz 266 5 to to TO cord-258014-lzzi4rnz 266 6 minimize minimize VB cord-258014-lzzi4rnz 266 7 sample sample NN cord-258014-lzzi4rnz 266 8 cross cross JJ cord-258014-lzzi4rnz 266 9 - - NN cord-258014-lzzi4rnz 266 10 contamination contamination NN cord-258014-lzzi4rnz 266 11 during during IN cord-258014-lzzi4rnz 266 12 evaporation evaporation NN cord-258014-lzzi4rnz 266 13 . . . cord-258014-lzzi4rnz 267 1 Preparation preparation NN cord-258014-lzzi4rnz 267 2 of of IN cord-258014-lzzi4rnz 267 3 the the DT cord-258014-lzzi4rnz 267 4 reference reference NN cord-258014-lzzi4rnz 267 5 pool pool NN cord-258014-lzzi4rnz 267 6 quality quality NN cord-258014-lzzi4rnz 267 7 control control NN cord-258014-lzzi4rnz 267 8 samples sample NNS cord-258014-lzzi4rnz 267 9 . . . cord-258014-lzzi4rnz 268 1 Prepare prepare VB cord-258014-lzzi4rnz 268 2 a a DT cord-258014-lzzi4rnz 268 3 large large JJ cord-258014-lzzi4rnz 268 4 pool pool NN cord-258014-lzzi4rnz 268 5 sample sample NN cord-258014-lzzi4rnz 268 6 during during IN cord-258014-lzzi4rnz 268 7 the the DT cord-258014-lzzi4rnz 268 8 preparation preparation NN cord-258014-lzzi4rnz 268 9 of of IN cord-258014-lzzi4rnz 268 10 sample sample NN cord-258014-lzzi4rnz 268 11 extracts extract NNS cord-258014-lzzi4rnz 268 12 by by IN cord-258014-lzzi4rnz 268 13 aliquoting aliquote VBG cord-258014-lzzi4rnz 268 14 100 100 CD cord-258014-lzzi4rnz 268 15 µl µl IN cord-258014-lzzi4rnz 268 16 from from IN cord-258014-lzzi4rnz 268 17 each each DT cord-258014-lzzi4rnz 268 18 1 1 CD cord-258014-lzzi4rnz 268 19 mL ml NN cord-258014-lzzi4rnz 268 20 authentic authentic JJ cord-258014-lzzi4rnz 268 21 subject subject NN cord-258014-lzzi4rnz 268 22 sample sample NN cord-258014-lzzi4rnz 268 23 . . . cord-258014-lzzi4rnz 269 1 After after IN cord-258014-lzzi4rnz 269 2 a a DT cord-258014-lzzi4rnz 269 3 collection collection NN cord-258014-lzzi4rnz 269 4 of of IN cord-258014-lzzi4rnz 269 5 a a DT cord-258014-lzzi4rnz 269 6 large large JJ cord-258014-lzzi4rnz 269 7 pool pool NN cord-258014-lzzi4rnz 269 8 sample sample NN cord-258014-lzzi4rnz 269 9 , , , cord-258014-lzzi4rnz 269 10 aliquot aliquot VBN cord-258014-lzzi4rnz 269 11 all all DT cord-258014-lzzi4rnz 269 12 for for IN cord-258014-lzzi4rnz 269 13 1 1 CD cord-258014-lzzi4rnz 269 14 mL ml NN cord-258014-lzzi4rnz 269 15 in in IN cord-258014-lzzi4rnz 269 16 separate separate JJ cord-258014-lzzi4rnz 269 17 vials vial NNS cord-258014-lzzi4rnz 269 18 ( ( -LRB- cord-258014-lzzi4rnz 269 19 thread thread NN cord-258014-lzzi4rnz 269 20 8 8 CD cord-258014-lzzi4rnz 269 21 - - SYM cord-258014-lzzi4rnz 269 22 425 425 CD cord-258014-lzzi4rnz 269 23 ) ) -RRB- cord-258014-lzzi4rnz 269 24 , , , cord-258014-lzzi4rnz 269 25 label label VB cord-258014-lzzi4rnz 269 26 all all DT cord-258014-lzzi4rnz 269 27 as as IN cord-258014-lzzi4rnz 269 28 reference reference NN cord-258014-lzzi4rnz 269 29 quality quality NN cord-258014-lzzi4rnz 269 30 controls control NNS cord-258014-lzzi4rnz 269 31 and and CC cord-258014-lzzi4rnz 269 32 process process VBP cord-258014-lzzi4rnz 269 33 similar similar JJ cord-258014-lzzi4rnz 269 34 to to IN cord-258014-lzzi4rnz 269 35 authentic authentic JJ cord-258014-lzzi4rnz 269 36 subject subject JJ cord-258014-lzzi4rnz 269 37 samples sample NNS cord-258014-lzzi4rnz 269 38 . . . cord-258014-lzzi4rnz 270 1 Continue continue VB cord-258014-lzzi4rnz 270 2 as as IN cord-258014-lzzi4rnz 270 3 instructed instruct VBN cord-258014-lzzi4rnz 270 4 in in IN cord-258014-lzzi4rnz 270 5 Section Section NNP cord-258014-lzzi4rnz 270 6 3.3.7 3.3.7 CD cord-258014-lzzi4rnz 270 7 . . . cord-258014-lzzi4rnz 271 1 The the DT cord-258014-lzzi4rnz 271 2 derivatization derivatization NN cord-258014-lzzi4rnz 271 3 solution solution NN cord-258014-lzzi4rnz 271 4 without without IN cord-258014-lzzi4rnz 271 5 the the DT cord-258014-lzzi4rnz 271 6 sample sample NN cord-258014-lzzi4rnz 271 7 only only RB cord-258014-lzzi4rnz 271 8 , , , cord-258014-lzzi4rnz 271 9 and and CC cord-258014-lzzi4rnz 271 10 empty empty JJ cord-258014-lzzi4rnz 271 11 vials vial NNS cord-258014-lzzi4rnz 271 12 , , , cord-258014-lzzi4rnz 271 13 must must MD cord-258014-lzzi4rnz 271 14 also also RB cord-258014-lzzi4rnz 271 15 be be VB cord-258014-lzzi4rnz 271 16 included include VBN cord-258014-lzzi4rnz 271 17 as as IN cord-258014-lzzi4rnz 271 18 quality quality NN cord-258014-lzzi4rnz 271 19 controls control NNS cord-258014-lzzi4rnz 271 20 in in IN cord-258014-lzzi4rnz 271 21 the the DT cord-258014-lzzi4rnz 271 22 analysis analysis NN cord-258014-lzzi4rnz 271 23 to to TO cord-258014-lzzi4rnz 271 24 ensure ensure VB cord-258014-lzzi4rnz 271 25 that that IN cord-258014-lzzi4rnz 271 26 identified identify VBN cord-258014-lzzi4rnz 271 27 metabolites metabolite NNS cord-258014-lzzi4rnz 271 28 are be VBP cord-258014-lzzi4rnz 271 29 derived derive VBN cord-258014-lzzi4rnz 271 30 from from IN cord-258014-lzzi4rnz 271 31 the the DT cord-258014-lzzi4rnz 271 32 sample sample NN cord-258014-lzzi4rnz 271 33 [ [ -LRB- cord-258014-lzzi4rnz 271 34 46 46 CD cord-258014-lzzi4rnz 271 35 ] ] -RRB- cord-258014-lzzi4rnz 271 36 . . . cord-258014-lzzi4rnz 272 1 Attach attach VB cord-258014-lzzi4rnz 272 2 the the DT cord-258014-lzzi4rnz 272 3 glass glass NN cord-258014-lzzi4rnz 272 4 vials vial NNS cord-258014-lzzi4rnz 272 5 to to IN cord-258014-lzzi4rnz 272 6 the the DT cord-258014-lzzi4rnz 272 7 rotary rotary JJ cord-258014-lzzi4rnz 272 8 evaporator evaporator NN cord-258014-lzzi4rnz 272 9 [ [ -LRB- cord-258014-lzzi4rnz 272 10 47 47 CD cord-258014-lzzi4rnz 272 11 ] ] -RRB- cord-258014-lzzi4rnz 272 12 . . . cord-258014-lzzi4rnz 273 1 6 6 LS cord-258014-lzzi4rnz 273 2 . . . cord-258014-lzzi4rnz 274 1 Derivatization Derivatization NNP cord-258014-lzzi4rnz 274 2 Solution Solution NNP cord-258014-lzzi4rnz 274 3 1 1 CD cord-258014-lzzi4rnz 274 4 must must MD cord-258014-lzzi4rnz 274 5 be be VB cord-258014-lzzi4rnz 274 6 freshly freshly RB cord-258014-lzzi4rnz 274 7 prepared prepare VBN cord-258014-lzzi4rnz 274 8 . . . cord-258014-lzzi4rnz 275 1 7 7 CD cord-258014-lzzi4rnz 275 2 . . . cord-258014-lzzi4rnz 276 1 During during IN cord-258014-lzzi4rnz 276 2 derivatization derivatization NN cord-258014-lzzi4rnz 276 3 , , , cord-258014-lzzi4rnz 276 4 avoid avoid VB cord-258014-lzzi4rnz 276 5 contact contact NN cord-258014-lzzi4rnz 276 6 with with IN cord-258014-lzzi4rnz 276 7 moisture moisture NN cord-258014-lzzi4rnz 276 8 , , , cord-258014-lzzi4rnz 276 9 which which WDT cord-258014-lzzi4rnz 276 10 will will MD cord-258014-lzzi4rnz 276 11 result result VB cord-258014-lzzi4rnz 276 12 in in IN cord-258014-lzzi4rnz 276 13 metabolite metabolite JJ cord-258014-lzzi4rnz 276 14 degradation degradation NN cord-258014-lzzi4rnz 276 15 . . . cord-258014-lzzi4rnz 277 1 If if IN cord-258014-lzzi4rnz 277 2 samples sample NNS cord-258014-lzzi4rnz 277 3 are be VBP cord-258014-lzzi4rnz 277 4 not not RB cord-258014-lzzi4rnz 277 5 dried dry VBN cord-258014-lzzi4rnz 277 6 , , , cord-258014-lzzi4rnz 277 7 it -PRON- PRP cord-258014-lzzi4rnz 277 8 is be VBZ cord-258014-lzzi4rnz 277 9 necessary necessary JJ cord-258014-lzzi4rnz 277 10 to to TO cord-258014-lzzi4rnz 277 11 proceed proceed VB cord-258014-lzzi4rnz 277 12 to to IN cord-258014-lzzi4rnz 277 13 additional additional JJ cord-258014-lzzi4rnz 277 14 evaporation evaporation NN cord-258014-lzzi4rnz 277 15 as as IN cord-258014-lzzi4rnz 277 16 said say VBN cord-258014-lzzi4rnz 277 17 in in IN cord-258014-lzzi4rnz 277 18 Sections section NNS cord-258014-lzzi4rnz 277 19 3.4.9 3.4.9 CD cord-258014-lzzi4rnz 277 20 - - HYPH cord-258014-lzzi4rnz 277 21 3.4.11 3.4.11 CD cord-258014-lzzi4rnz 277 22 . . . cord-258014-lzzi4rnz 278 1 8 8 CD cord-258014-lzzi4rnz 278 2 . . . cord-258014-lzzi4rnz 279 1 Derivatization Derivatization NNP cord-258014-lzzi4rnz 279 2 Solution Solution NNP cord-258014-lzzi4rnz 279 3 2 2 CD cord-258014-lzzi4rnz 279 4 must must MD cord-258014-lzzi4rnz 279 5 be be VB cord-258014-lzzi4rnz 279 6 used use VBN cord-258014-lzzi4rnz 279 7 from from IN cord-258014-lzzi4rnz 279 8 freshly freshly RB cord-258014-lzzi4rnz 279 9 opened open VBN cord-258014-lzzi4rnz 279 10 vials vial NNS cord-258014-lzzi4rnz 279 11 . . . cord-258014-lzzi4rnz 280 1 9 9 LS cord-258014-lzzi4rnz 280 2 . . . cord-258014-lzzi4rnz 281 1 If if IN cord-258014-lzzi4rnz 281 2 an an DT cord-258014-lzzi4rnz 281 3 analysis analysis NN cord-258014-lzzi4rnz 281 4 is be VBZ cord-258014-lzzi4rnz 281 5 performed perform VBN cord-258014-lzzi4rnz 281 6 after after IN cord-258014-lzzi4rnz 281 7 samples sample NNS cord-258014-lzzi4rnz 281 8 preparation preparation NN cord-258014-lzzi4rnz 281 9 , , , cord-258014-lzzi4rnz 281 10 wait wait VB cord-258014-lzzi4rnz 281 11 two two CD cord-258014-lzzi4rnz 281 12 hours hour NNS cord-258014-lzzi4rnz 281 13 before before IN cord-258014-lzzi4rnz 281 14 injecting inject VBG cord-258014-lzzi4rnz 281 15 the the DT cord-258014-lzzi4rnz 281 16 first first JJ cord-258014-lzzi4rnz 281 17 sample sample NN cord-258014-lzzi4rnz 281 18 into into IN cord-258014-lzzi4rnz 281 19 the the DT cord-258014-lzzi4rnz 281 20 GC GC NNP cord-258014-lzzi4rnz 281 21 - - HYPH cord-258014-lzzi4rnz 281 22 MS MS NNP cord-258014-lzzi4rnz 281 23 . . . cord-258014-lzzi4rnz 282 1 10 10 CD cord-258014-lzzi4rnz 282 2 . . . cord-258014-lzzi4rnz 283 1 The the DT cord-258014-lzzi4rnz 283 2 sample sample NN cord-258014-lzzi4rnz 283 3 concentration concentration NN cord-258014-lzzi4rnz 283 4 must must MD cord-258014-lzzi4rnz 283 5 be be VB cord-258014-lzzi4rnz 283 6 adequately adequately RB cord-258014-lzzi4rnz 283 7 adjusted adjust VBN cord-258014-lzzi4rnz 283 8 to to TO cord-258014-lzzi4rnz 283 9 give give VB cord-258014-lzzi4rnz 283 10 sufficient sufficient JJ cord-258014-lzzi4rnz 283 11 chromatogram chromatogram NN cord-258014-lzzi4rnz 283 12 , , , cord-258014-lzzi4rnz 283 13 then then RB cord-258014-lzzi4rnz 283 14 it -PRON- PRP cord-258014-lzzi4rnz 283 15 needs need VBZ cord-258014-lzzi4rnz 283 16 to to TO cord-258014-lzzi4rnz 283 17 be be VB cord-258014-lzzi4rnz 283 18 concentrated concentrate VBN cord-258014-lzzi4rnz 283 19 before before IN cord-258014-lzzi4rnz 283 20 injecting inject VBG cord-258014-lzzi4rnz 283 21 the the DT cord-258014-lzzi4rnz 283 22 sample sample NN cord-258014-lzzi4rnz 283 23 into into IN cord-258014-lzzi4rnz 283 24 the the DT cord-258014-lzzi4rnz 283 25 gas gas NN cord-258014-lzzi4rnz 283 26 chromatograph chromatograph NN cord-258014-lzzi4rnz 283 27 . . . cord-258014-lzzi4rnz 284 1 The the DT cord-258014-lzzi4rnz 284 2 dilution dilution NN cord-258014-lzzi4rnz 284 3 of of IN cord-258014-lzzi4rnz 284 4 a a DT cord-258014-lzzi4rnz 284 5 sample sample NN cord-258014-lzzi4rnz 284 6 ( ( -LRB- cord-258014-lzzi4rnz 284 7 1:50 1:50 CD cord-258014-lzzi4rnz 284 8 ) ) -RRB- cord-258014-lzzi4rnz 284 9 was be VBD cord-258014-lzzi4rnz 284 10 found find VBN cord-258014-lzzi4rnz 284 11 to to TO cord-258014-lzzi4rnz 284 12 be be VB cord-258014-lzzi4rnz 284 13 sufficient sufficient JJ cord-258014-lzzi4rnz 284 14 for for IN cord-258014-lzzi4rnz 284 15 the the DT cord-258014-lzzi4rnz 284 16 analysis analysis NN cord-258014-lzzi4rnz 284 17 . . . cord-258014-lzzi4rnz 285 1 However however RB cord-258014-lzzi4rnz 285 2 , , , cord-258014-lzzi4rnz 285 3 if if IN cord-258014-lzzi4rnz 285 4 the the DT cord-258014-lzzi4rnz 285 5 signal signal NN cord-258014-lzzi4rnz 285 6 is be VBZ cord-258014-lzzi4rnz 285 7 too too RB cord-258014-lzzi4rnz 285 8 low low JJ cord-258014-lzzi4rnz 285 9 , , , cord-258014-lzzi4rnz 285 10 the the DT cord-258014-lzzi4rnz 285 11 concentration concentration NN cord-258014-lzzi4rnz 285 12 could could MD cord-258014-lzzi4rnz 285 13 be be VB cord-258014-lzzi4rnz 285 14 increased increase VBN cord-258014-lzzi4rnz 285 15 . . . cord-258014-lzzi4rnz 286 1 Derivatization derivatization NN cord-258014-lzzi4rnz 286 2 solution solution NN cord-258014-lzzi4rnz 286 3 without without IN cord-258014-lzzi4rnz 286 4 the the DT cord-258014-lzzi4rnz 286 5 sample sample NN cord-258014-lzzi4rnz 286 6 only only RB cord-258014-lzzi4rnz 286 7 , , , cord-258014-lzzi4rnz 286 8 and and CC cord-258014-lzzi4rnz 286 9 empty empty JJ cord-258014-lzzi4rnz 286 10 vials vial NNS cord-258014-lzzi4rnz 286 11 , , , cord-258014-lzzi4rnz 286 12 must must MD cord-258014-lzzi4rnz 286 13 also also RB cord-258014-lzzi4rnz 286 14 be be VB cord-258014-lzzi4rnz 286 15 included include VBN cord-258014-lzzi4rnz 286 16 as as IN cord-258014-lzzi4rnz 286 17 quality quality NN cord-258014-lzzi4rnz 286 18 controls control NNS cord-258014-lzzi4rnz 286 19 in in IN cord-258014-lzzi4rnz 286 20 the the DT cord-258014-lzzi4rnz 286 21 analysis analysis NN cord-258014-lzzi4rnz 286 22 to to TO cord-258014-lzzi4rnz 286 23 ensure ensure VB cord-258014-lzzi4rnz 286 24 that that IN cord-258014-lzzi4rnz 286 25 identified identify VBN cord-258014-lzzi4rnz 286 26 metabolites metabolite NNS cord-258014-lzzi4rnz 286 27 11 11 CD cord-258014-lzzi4rnz 286 28 . . . cord-258014-lzzi4rnz 287 1 MS MS NNP cord-258014-lzzi4rnz 287 2 detects detect VBZ cord-258014-lzzi4rnz 287 3 the the DT cord-258014-lzzi4rnz 287 4 mass mass NN cord-258014-lzzi4rnz 287 5 of of IN cord-258014-lzzi4rnz 287 6 the the DT cord-258014-lzzi4rnz 287 7 molecular molecular JJ cord-258014-lzzi4rnz 287 8 ions ion NNS cord-258014-lzzi4rnz 287 9 and and CC cord-258014-lzzi4rnz 287 10 the the DT cord-258014-lzzi4rnz 287 11 masses masse NNS cord-258014-lzzi4rnz 287 12 of of IN cord-258014-lzzi4rnz 287 13 the the DT cord-258014-lzzi4rnz 287 14 fragment fragment NN cord-258014-lzzi4rnz 287 15 ions ion NNS cord-258014-lzzi4rnz 287 16 . . . cord-258014-lzzi4rnz 288 1 The the DT cord-258014-lzzi4rnz 288 2 database database NN cord-258014-lzzi4rnz 288 3 contains contain VBZ cord-258014-lzzi4rnz 288 4 extensive extensive JJ cord-258014-lzzi4rnz 288 5 information information NN cord-258014-lzzi4rnz 288 6 on on IN cord-258014-lzzi4rnz 288 7 many many JJ cord-258014-lzzi4rnz 288 8 compounds compound NNS cord-258014-lzzi4rnz 288 9 including include VBG cord-258014-lzzi4rnz 288 10 the the DT cord-258014-lzzi4rnz 288 11 masses masse NNS cord-258014-lzzi4rnz 288 12 of of IN cord-258014-lzzi4rnz 288 13 fragment fragment NN cord-258014-lzzi4rnz 288 14 ions ion NNS cord-258014-lzzi4rnz 288 15 that that WDT cord-258014-lzzi4rnz 288 16 help help VBP cord-258014-lzzi4rnz 288 17 to to TO cord-258014-lzzi4rnz 288 18 perform perform VB cord-258014-lzzi4rnz 288 19 successful successful JJ cord-258014-lzzi4rnz 288 20 metabolite metabolite JJ cord-258014-lzzi4rnz 288 21 identification identification NN cord-258014-lzzi4rnz 288 22 . . . cord-258014-lzzi4rnz 289 1 In in IN cord-258014-lzzi4rnz 289 2 addition addition NN cord-258014-lzzi4rnz 289 3 , , , cord-258014-lzzi4rnz 289 4 to to TO cord-258014-lzzi4rnz 289 5 ensure ensure VB cord-258014-lzzi4rnz 289 6 a a DT cord-258014-lzzi4rnz 289 7 valid valid JJ cord-258014-lzzi4rnz 289 8 peak peak NN cord-258014-lzzi4rnz 289 9 identification identification NN cord-258014-lzzi4rnz 289 10 , , , cord-258014-lzzi4rnz 289 11 data datum NNS cord-258014-lzzi4rnz 289 12 can can MD cord-258014-lzzi4rnz 289 13 be be VB cord-258014-lzzi4rnz 289 14 also also RB cord-258014-lzzi4rnz 289 15 processed process VBN cord-258014-lzzi4rnz 289 16 using use VBG cord-258014-lzzi4rnz 289 17 a a DT cord-258014-lzzi4rnz 289 18 deconvolution deconvolution NN cord-258014-lzzi4rnz 289 19 method method NN cord-258014-lzzi4rnz 289 20 , , , cord-258014-lzzi4rnz 289 21 which which WDT cord-258014-lzzi4rnz 289 22 is be VBZ cord-258014-lzzi4rnz 289 23 a a DT cord-258014-lzzi4rnz 289 24 signal signal JJ cord-258014-lzzi4rnz 289 25 processing processing NN cord-258014-lzzi4rnz 289 26 technique technique NN cord-258014-lzzi4rnz 289 27 that that WDT cord-258014-lzzi4rnz 289 28 estimates estimate VBZ cord-258014-lzzi4rnz 289 29 the the DT cord-258014-lzzi4rnz 289 30 relative relative JJ cord-258014-lzzi4rnz 289 31 area area NN cord-258014-lzzi4rnz 289 32 corresponding correspond VBG cord-258014-lzzi4rnz 289 33 to to IN cord-258014-lzzi4rnz 289 34 each each DT cord-258014-lzzi4rnz 289 35 peak peak NN cord-258014-lzzi4rnz 289 36 when when WRB cord-258014-lzzi4rnz 289 37 multiple multiple JJ cord-258014-lzzi4rnz 289 38 peaks peak NNS cord-258014-lzzi4rnz 289 39 overlap overlap VBP cord-258014-lzzi4rnz 289 40 within within IN cord-258014-lzzi4rnz 289 41 the the DT cord-258014-lzzi4rnz 289 42 same same JJ cord-258014-lzzi4rnz 289 43 spectral spectral JJ cord-258014-lzzi4rnz 289 44 region region NN cord-258014-lzzi4rnz 289 45 . . . cord-258014-lzzi4rnz 290 1 This this DT cord-258014-lzzi4rnz 290 2 is be VBZ cord-258014-lzzi4rnz 290 3 especially especially RB cord-258014-lzzi4rnz 290 4 important important JJ cord-258014-lzzi4rnz 290 5 for for IN cord-258014-lzzi4rnz 290 6 low low JJ cord-258014-lzzi4rnz 290 7 abundant abundant JJ cord-258014-lzzi4rnz 290 8 metabolites metabolite NNS cord-258014-lzzi4rnz 290 9 that that WDT cord-258014-lzzi4rnz 290 10 might may MD cord-258014-lzzi4rnz 290 11 co co VB cord-258014-lzzi4rnz 290 12 - - VB cord-258014-lzzi4rnz 290 13 elute elute VB cord-258014-lzzi4rnz 290 14 with with IN cord-258014-lzzi4rnz 290 15 abundant abundant JJ cord-258014-lzzi4rnz 290 16 major major JJ cord-258014-lzzi4rnz 290 17 peaks peak NNS cord-258014-lzzi4rnz 290 18 . . . cord-258014-lzzi4rnz 291 1 For for IN cord-258014-lzzi4rnz 291 2 general general JJ cord-258014-lzzi4rnz 291 3 quadrupole quadrupole JJ cord-258014-lzzi4rnz 291 4 mass mass NN cord-258014-lzzi4rnz 291 5 spectrometers spectrometer NNS cord-258014-lzzi4rnz 291 6 , , , cord-258014-lzzi4rnz 291 7 data datum NNS cord-258014-lzzi4rnz 291 8 deconvolution deconvolution NN cord-258014-lzzi4rnz 291 9 by by IN cord-258014-lzzi4rnz 291 10 the the DT cord-258014-lzzi4rnz 291 11 freely freely RB cord-258014-lzzi4rnz 291 12 available available JJ cord-258014-lzzi4rnz 291 13 software software NN cord-258014-lzzi4rnz 291 14 AMDIS AMDIS NNP cord-258014-lzzi4rnz 291 15 is be VBZ cord-258014-lzzi4rnz 291 16 recommended recommend VBN cord-258014-lzzi4rnz 291 17 ( ( -LRB- cord-258014-lzzi4rnz 291 18 http://chemdata.nist.gov/mass-spc/amdis/ http://chemdata.nist.gov/mass-spc/amdis/ ADD cord-258014-lzzi4rnz 291 19 ) ) -RRB- cord-258014-lzzi4rnz 291 20 . . . cord-258014-lzzi4rnz 292 1 12 12 CD cord-258014-lzzi4rnz 292 2 . . . cord-258014-lzzi4rnz 293 1 Some some DT cord-258014-lzzi4rnz 293 2 of of IN cord-258014-lzzi4rnz 293 3 the the DT cord-258014-lzzi4rnz 293 4 metabolite metabolite NN cord-258014-lzzi4rnz 293 5 's 's POS cord-258014-lzzi4rnz 293 6 so so RB cord-258014-lzzi4rnz 293 7 - - HYPH cord-258014-lzzi4rnz 293 8 called call VBN cord-258014-lzzi4rnz 293 9 " " `` cord-258014-lzzi4rnz 293 10 missing missing JJ cord-258014-lzzi4rnz 293 11 values value NNS cord-258014-lzzi4rnz 293 12 " " '' cord-258014-lzzi4rnz 293 13 will will MD cord-258014-lzzi4rnz 293 14 not not RB cord-258014-lzzi4rnz 293 15 be be VB cord-258014-lzzi4rnz 293 16 identified identify VBN cord-258014-lzzi4rnz 293 17 in in IN cord-258014-lzzi4rnz 293 18 chromatograms chromatogram NNS cord-258014-lzzi4rnz 293 19 . . . cord-258014-lzzi4rnz 294 1 Therefore therefore RB cord-258014-lzzi4rnz 294 2 , , , cord-258014-lzzi4rnz 294 3 when when WRB cord-258014-lzzi4rnz 294 4 composing compose VBG cord-258014-lzzi4rnz 294 5 the the DT cord-258014-lzzi4rnz 294 6 table table NN cord-258014-lzzi4rnz 294 7 for for IN cord-258014-lzzi4rnz 294 8 the the DT cord-258014-lzzi4rnz 294 9 analysis analysis NN cord-258014-lzzi4rnz 294 10 , , , cord-258014-lzzi4rnz 294 11 missing miss VBG cord-258014-lzzi4rnz 294 12 values value NNS cord-258014-lzzi4rnz 294 13 should should MD cord-258014-lzzi4rnz 294 14 be be VB cord-258014-lzzi4rnz 294 15 presented present VBN cord-258014-lzzi4rnz 294 16 as as IN cord-258014-lzzi4rnz 294 17 empty empty JJ cord-258014-lzzi4rnz 294 18 values value NNS cord-258014-lzzi4rnz 294 19 or or CC cord-258014-lzzi4rnz 294 20 NA NA NNP cord-258014-lzzi4rnz 294 21 without without IN cord-258014-lzzi4rnz 294 22 quotes quote NNS cord-258014-lzzi4rnz 294 23 . . . cord-258014-lzzi4rnz 295 1 13 13 CD cord-258014-lzzi4rnz 295 2 . . . cord-258014-lzzi4rnz 296 1 MetaboAnalyst MetaboAnalyst NNP cord-258014-lzzi4rnz 296 2 is be VBZ cord-258014-lzzi4rnz 296 3 a a DT cord-258014-lzzi4rnz 296 4 web web NN cord-258014-lzzi4rnz 296 5 server server NN cord-258014-lzzi4rnz 296 6 designed design VBN cord-258014-lzzi4rnz 296 7 to to TO cord-258014-lzzi4rnz 296 8 permit permit VB cord-258014-lzzi4rnz 296 9 comprehensive comprehensive JJ cord-258014-lzzi4rnz 296 10 metabolomics metabolomic NNS cord-258014-lzzi4rnz 296 11 data data NN cord-258014-lzzi4rnz 296 12 analysis analysis NN cord-258014-lzzi4rnz 296 13 , , , cord-258014-lzzi4rnz 296 14 visualization visualization NN cord-258014-lzzi4rnz 296 15 , , , cord-258014-lzzi4rnz 296 16 and and CC cord-258014-lzzi4rnz 296 17 interpretation interpretation NN cord-258014-lzzi4rnz 296 18 [ [ -LRB- cord-258014-lzzi4rnz 296 19 39 39 CD cord-258014-lzzi4rnz 296 20 ] ] -RRB- cord-258014-lzzi4rnz 296 21 . . . cord-258014-lzzi4rnz 297 1 The the DT cord-258014-lzzi4rnz 297 2 web web NN cord-258014-lzzi4rnz 297 3 interfaces interface NNS cord-258014-lzzi4rnz 297 4 of of IN cord-258014-lzzi4rnz 297 5 MetaboAnalyst MetaboAnalyst NNP cord-258014-lzzi4rnz 297 6 are be VBP cord-258014-lzzi4rnz 297 7 designed design VBN cord-258014-lzzi4rnz 297 8 to to TO cord-258014-lzzi4rnz 297 9 be be VB cord-258014-lzzi4rnz 297 10 self self NN cord-258014-lzzi4rnz 297 11 - - HYPH cord-258014-lzzi4rnz 297 12 explanatory explanatory JJ cord-258014-lzzi4rnz 297 13 . . . cord-258014-lzzi4rnz 298 1 Therefore therefore RB cord-258014-lzzi4rnz 298 2 , , , cord-258014-lzzi4rnz 298 3 most most JJS cord-258014-lzzi4rnz 298 4 steps step NNS cord-258014-lzzi4rnz 298 5 are be VBP cord-258014-lzzi4rnz 298 6 documented document VBN cord-258014-lzzi4rnz 298 7 on on IN cord-258014-lzzi4rnz 298 8 top top NN cord-258014-lzzi4rnz 298 9 of of IN cord-258014-lzzi4rnz 298 10 the the DT cord-258014-lzzi4rnz 298 11 corresponding correspond VBG cord-258014-lzzi4rnz 298 12 pages page NNS cord-258014-lzzi4rnz 298 13 . . . cord-258014-lzzi4rnz 299 1 Available available JJ cord-258014-lzzi4rnz 299 2 tutorials tutorial NNS cord-258014-lzzi4rnz 299 3 and and CC cord-258014-lzzi4rnz 299 4 sample sample NN cord-258014-lzzi4rnz 299 5 data data NN cord-258014-lzzi4rnz 299 6 sets set NNS cord-258014-lzzi4rnz 299 7 complement complement VBP cord-258014-lzzi4rnz 299 8 the the DT cord-258014-lzzi4rnz 299 9 information information NN cord-258014-lzzi4rnz 299 10 by by IN cord-258014-lzzi4rnz 299 11 providing provide VBG cord-258014-lzzi4rnz 299 12 step step NN cord-258014-lzzi4rnz 299 13 - - HYPH cord-258014-lzzi4rnz 299 14 by by IN cord-258014-lzzi4rnz 299 15 - - HYPH cord-258014-lzzi4rnz 299 16 step step NN cord-258014-lzzi4rnz 299 17 instructions instruction NNS cord-258014-lzzi4rnz 299 18 for for IN cord-258014-lzzi4rnz 299 19 several several JJ cord-258014-lzzi4rnz 299 20 most most JJS cord-258014-lzzi4rnz 299 21 common common JJ cord-258014-lzzi4rnz 299 22 tasks task NNS cord-258014-lzzi4rnz 299 23 . . . cord-258014-lzzi4rnz 300 1 14 14 CD cord-258014-lzzi4rnz 300 2 . . . cord-258014-lzzi4rnz 301 1 This this DT cord-258014-lzzi4rnz 301 2 step step NN cord-258014-lzzi4rnz 301 3 is be VBZ cord-258014-lzzi4rnz 301 4 strongly strongly RB cord-258014-lzzi4rnz 301 5 recommended recommend VBN cord-258014-lzzi4rnz 301 6 for for IN cord-258014-lzzi4rnz 301 7 metabolomics metabolomic NNS cord-258014-lzzi4rnz 301 8 datasets dataset NNS cord-258014-lzzi4rnz 301 9 with with IN cord-258014-lzzi4rnz 301 10 a a DT cord-258014-lzzi4rnz 301 11 large large JJ cord-258014-lzzi4rnz 301 12 number number NN cord-258014-lzzi4rnz 301 13 of of IN cord-258014-lzzi4rnz 301 14 variables variable NNS cord-258014-lzzi4rnz 301 15 ( ( -LRB- cord-258014-lzzi4rnz 301 16 more more JJR cord-258014-lzzi4rnz 301 17 than than IN cord-258014-lzzi4rnz 301 18 2000 2000 CD cord-258014-lzzi4rnz 301 19 metabolites metabolite NNS cord-258014-lzzi4rnz 301 20 ) ) -RRB- cord-258014-lzzi4rnz 301 21 since since IN cord-258014-lzzi4rnz 301 22 many many JJ cord-258014-lzzi4rnz 301 23 of of IN cord-258014-lzzi4rnz 301 24 them -PRON- PRP cord-258014-lzzi4rnz 301 25 are be VBP cord-258014-lzzi4rnz 301 26 from from IN cord-258014-lzzi4rnz 301 27 baseline baseline JJ cord-258014-lzzi4rnz 301 28 noises noise NNS cord-258014-lzzi4rnz 301 29 . . . cord-258014-lzzi4rnz 302 1 Usually usually RB cord-258014-lzzi4rnz 302 2 , , , cord-258014-lzzi4rnz 302 3 the the DT cord-258014-lzzi4rnz 302 4 GC GC NNP cord-258014-lzzi4rnz 302 5 / / SYM cord-258014-lzzi4rnz 302 6 MS MS NNP cord-258014-lzzi4rnz 302 7 analysis analysis NN cord-258014-lzzi4rnz 302 8 of of IN cord-258014-lzzi4rnz 302 9 brain brain NN cord-258014-lzzi4rnz 302 10 tissues tissue NNS cord-258014-lzzi4rnz 302 11 could could MD cord-258014-lzzi4rnz 302 12 identify identify VB cord-258014-lzzi4rnz 302 13 less less JJR cord-258014-lzzi4rnz 302 14 than than IN cord-258014-lzzi4rnz 302 15 60 60 CD cord-258014-lzzi4rnz 302 16 variables variable NNS cord-258014-lzzi4rnz 302 17 . . . cord-258014-lzzi4rnz 303 1 15 15 CD cord-258014-lzzi4rnz 303 2 . . . cord-258014-lzzi4rnz 304 1 The the DT cord-258014-lzzi4rnz 304 2 normalization normalization NN cord-258014-lzzi4rnz 304 3 procedures procedure NNS cord-258014-lzzi4rnz 304 4 are be VBP cord-258014-lzzi4rnz 304 5 grouped group VBN cord-258014-lzzi4rnz 304 6 into into IN cord-258014-lzzi4rnz 304 7 three three CD cord-258014-lzzi4rnz 304 8 categories category NNS cord-258014-lzzi4rnz 304 9 . . . cord-258014-lzzi4rnz 305 1 The the DT cord-258014-lzzi4rnz 305 2 sample sample NN cord-258014-lzzi4rnz 305 3 normalization normalization NN cord-258014-lzzi4rnz 305 4 allows allow VBZ cord-258014-lzzi4rnz 305 5 general general JJ cord-258014-lzzi4rnz 305 6 - - HYPH cord-258014-lzzi4rnz 305 7 purpose purpose NN cord-258014-lzzi4rnz 305 8 adjustment adjustment NN cord-258014-lzzi4rnz 305 9 for for IN cord-258014-lzzi4rnz 305 10 differences difference NNS cord-258014-lzzi4rnz 305 11 among among IN cord-258014-lzzi4rnz 305 12 your -PRON- PRP$ cord-258014-lzzi4rnz 305 13 sample sample NN cord-258014-lzzi4rnz 305 14 , , , cord-258014-lzzi4rnz 305 15 which which WDT cord-258014-lzzi4rnz 305 16 can can MD cord-258014-lzzi4rnz 305 17 include include VB cord-258014-lzzi4rnz 305 18 the the DT cord-258014-lzzi4rnz 305 19 protein protein NN cord-258014-lzzi4rnz 305 20 content content NN cord-258014-lzzi4rnz 305 21 in in IN cord-258014-lzzi4rnz 305 22 the the DT cord-258014-lzzi4rnz 305 23 sample sample NN cord-258014-lzzi4rnz 305 24 or or CC cord-258014-lzzi4rnz 305 25 intensity intensity NN cord-258014-lzzi4rnz 305 26 value value NN cord-258014-lzzi4rnz 305 27 of of IN cord-258014-lzzi4rnz 305 28 internal internal JJ cord-258014-lzzi4rnz 305 29 standard standard NN cord-258014-lzzi4rnz 305 30 spiked spike VBD cord-258014-lzzi4rnz 305 31 into into IN cord-258014-lzzi4rnz 305 32 the the DT cord-258014-lzzi4rnz 305 33 derivatization derivatization NN cord-258014-lzzi4rnz 305 34 mixture mixture NN cord-258014-lzzi4rnz 305 35 before before IN cord-258014-lzzi4rnz 305 36 GC GC NNP cord-258014-lzzi4rnz 305 37 / / SYM cord-258014-lzzi4rnz 305 38 MS MS NNP cord-258014-lzzi4rnz 305 39 analysis analysis NN cord-258014-lzzi4rnz 305 40 . . . cord-258014-lzzi4rnz 306 1 Data datum NNS cord-258014-lzzi4rnz 306 2 can can MD cord-258014-lzzi4rnz 306 3 be be VB cord-258014-lzzi4rnz 306 4 further further RB cord-258014-lzzi4rnz 306 5 transformed transform VBN cord-258014-lzzi4rnz 306 6 by by IN cord-258014-lzzi4rnz 306 7 " " `` cord-258014-lzzi4rnz 306 8 log log NN cord-258014-lzzi4rnz 306 9 " " '' cord-258014-lzzi4rnz 306 10 or or CC cord-258014-lzzi4rnz 306 11 " " `` cord-258014-lzzi4rnz 306 12 cube cube NN cord-258014-lzzi4rnz 306 13 root root NN cord-258014-lzzi4rnz 306 14 " " '' cord-258014-lzzi4rnz 306 15 and/or and/or CC cord-258014-lzzi4rnz 306 16 scaled scale VBN cord-258014-lzzi4rnz 306 17 via via IN cord-258014-lzzi4rnz 306 18 " " `` cord-258014-lzzi4rnz 306 19 mean mean JJ cord-258014-lzzi4rnz 306 20 centering centering NN cord-258014-lzzi4rnz 306 21 , , , cord-258014-lzzi4rnz 306 22 autoscaling autoscaling NN cord-258014-lzzi4rnz 306 23 , , , cord-258014-lzzi4rnz 306 24 Pareto Pareto NNP cord-258014-lzzi4rnz 306 25 scaling scaling NN cord-258014-lzzi4rnz 306 26 , , , cord-258014-lzzi4rnz 306 27 and and CC cord-258014-lzzi4rnz 306 28 range range VB cord-258014-lzzi4rnz 306 29 scaling scaling NN cord-258014-lzzi4rnz 306 30 " " '' cord-258014-lzzi4rnz 306 31 ( ( -LRB- cord-258014-lzzi4rnz 306 32 please please UH cord-258014-lzzi4rnz 306 33 see see VB cord-258014-lzzi4rnz 306 34 the the DT cord-258014-lzzi4rnz 306 35 description description NN cord-258014-lzzi4rnz 306 36 of of IN cord-258014-lzzi4rnz 306 37 each each DT cord-258014-lzzi4rnz 306 38 in in IN cord-258014-lzzi4rnz 306 39 the the DT cord-258014-lzzi4rnz 306 40 MetaboAnalyst MetaboAnalyst NNP cord-258014-lzzi4rnz 306 41 program program NN cord-258014-lzzi4rnz 306 42 ) ) -RRB- cord-258014-lzzi4rnz 306 43 . . . cord-258014-lzzi4rnz 307 1 According accord VBG cord-258014-lzzi4rnz 307 2 to to IN cord-258014-lzzi4rnz 307 3 [ [ -LRB- cord-258014-lzzi4rnz 307 4 48 48 CD cord-258014-lzzi4rnz 307 5 ] ] -RRB- cord-258014-lzzi4rnz 307 6 autoscaling autoscaling NN cord-258014-lzzi4rnz 307 7 and and CC cord-258014-lzzi4rnz 307 8 range range NN cord-258014-lzzi4rnz 307 9 scaling scale VBG cord-258014-lzzi4rnz 307 10 performed perform VBN cord-258014-lzzi4rnz 307 11 better well RBR cord-258014-lzzi4rnz 307 12 than than IN cord-258014-lzzi4rnz 307 13 the the DT cord-258014-lzzi4rnz 307 14 other other JJ cord-258014-lzzi4rnz 307 15 pretreatment pretreatment NN cord-258014-lzzi4rnz 307 16 methods method NNS cord-258014-lzzi4rnz 307 17 and and CC cord-258014-lzzi4rnz 307 18 showed show VBD cord-258014-lzzi4rnz 307 19 biologically biologically RB cord-258014-lzzi4rnz 307 20 sensible sensible JJ cord-258014-lzzi4rnz 307 21 results result NNS cord-258014-lzzi4rnz 307 22 in in IN cord-258014-lzzi4rnz 307 23 chemometrics chemometric NNS cord-258014-lzzi4rnz 307 24 analysis analysis NN cord-258014-lzzi4rnz 307 25 . . . cord-258014-lzzi4rnz 308 1 Data data NN cord-258014-lzzi4rnz 308 2 transformation transformation NN cord-258014-lzzi4rnz 308 3 and and CC cord-258014-lzzi4rnz 308 4 scaling scaling NN cord-258014-lzzi4rnz 308 5 are be VBP cord-258014-lzzi4rnz 308 6 two two CD cord-258014-lzzi4rnz 308 7 different different JJ cord-258014-lzzi4rnz 308 8 approaches approach NNS cord-258014-lzzi4rnz 308 9 to to TO cord-258014-lzzi4rnz 308 10 make make VB cord-258014-lzzi4rnz 308 11 individual individual JJ cord-258014-lzzi4rnz 308 12 features feature VBZ cord-258014-lzzi4rnz 308 13 more more RBR cord-258014-lzzi4rnz 308 14 comparable comparable JJ cord-258014-lzzi4rnz 308 15 that that WDT cord-258014-lzzi4rnz 308 16 can can MD cord-258014-lzzi4rnz 308 17 be be VB cord-258014-lzzi4rnz 308 18 used use VBN cord-258014-lzzi4rnz 308 19 individually individually RB cord-258014-lzzi4rnz 308 20 or or CC cord-258014-lzzi4rnz 308 21 in in IN cord-258014-lzzi4rnz 308 22 combination combination NN cord-258014-lzzi4rnz 308 23 . . . cord-258014-lzzi4rnz 309 1 Scaling scaling NN cord-258014-lzzi4rnz 309 2 is be VBZ cord-258014-lzzi4rnz 309 3 a a DT cord-258014-lzzi4rnz 309 4 useful useful JJ cord-258014-lzzi4rnz 309 5 procedure procedure NN cord-258014-lzzi4rnz 309 6 when when WRB cord-258014-lzzi4rnz 309 7 variables variable NNS cord-258014-lzzi4rnz 309 8 are be VBP cord-258014-lzzi4rnz 309 9 of of IN cord-258014-lzzi4rnz 309 10 very very RB cord-258014-lzzi4rnz 309 11 different different JJ cord-258014-lzzi4rnz 309 12 orders order NNS cord-258014-lzzi4rnz 309 13 of of IN cord-258014-lzzi4rnz 309 14 magnitude magnitude NN cord-258014-lzzi4rnz 309 15 [ [ -LRB- cord-258014-lzzi4rnz 309 16 49 49 CD cord-258014-lzzi4rnz 309 17 ] ] -RRB- cord-258014-lzzi4rnz 309 18 . . . cord-258014-lzzi4rnz 310 1 16 16 CD cord-258014-lzzi4rnz 310 2 . . . cord-258014-lzzi4rnz 311 1 Detect detect VB cord-258014-lzzi4rnz 311 2 outliers outlier NNS cord-258014-lzzi4rnz 311 3 . . . cord-258014-lzzi4rnz 312 1 This this DT cord-258014-lzzi4rnz 312 2 can can MD cord-258014-lzzi4rnz 312 3 be be VB cord-258014-lzzi4rnz 312 4 carried carry VBN cord-258014-lzzi4rnz 312 5 out out RP cord-258014-lzzi4rnz 312 6 by by IN cord-258014-lzzi4rnz 312 7 visual visual JJ cord-258014-lzzi4rnz 312 8 inspection inspection NN cord-258014-lzzi4rnz 312 9 of of IN cord-258014-lzzi4rnz 312 10 the the DT cord-258014-lzzi4rnz 312 11 PCA PCA NNP cord-258014-lzzi4rnz 312 12 plot plot NN cord-258014-lzzi4rnz 312 13 to to TO cord-258014-lzzi4rnz 312 14 identify identify VB cord-258014-lzzi4rnz 312 15 samples sample NNS cord-258014-lzzi4rnz 312 16 - - : cord-258014-lzzi4rnz 312 17 outliers outlier NNS cord-258014-lzzi4rnz 312 18 outside outside IN cord-258014-lzzi4rnz 312 19 the the DT cord-258014-lzzi4rnz 312 20 Hotelling Hotelling NNP cord-258014-lzzi4rnz 312 21 T2 T2 NNP cord-258014-lzzi4rnz 312 22 95 95 CD cord-258014-lzzi4rnz 312 23 % % NN cord-258014-lzzi4rnz 312 24 confidence confidence NN cord-258014-lzzi4rnz 312 25 ellipse ellipse NN cord-258014-lzzi4rnz 312 26 [ [ -LRB- cord-258014-lzzi4rnz 312 27 40 40 CD cord-258014-lzzi4rnz 312 28 ] ] -RRB- cord-258014-lzzi4rnz 312 29 . . . cord-258014-lzzi4rnz 313 1 Many many JJ cord-258014-lzzi4rnz 313 2 outliers outlier NNS cord-258014-lzzi4rnz 313 3 could could MD cord-258014-lzzi4rnz 313 4 be be VB cord-258014-lzzi4rnz 313 5 corrected correct VBN cord-258014-lzzi4rnz 313 6 by by IN cord-258014-lzzi4rnz 313 7 normalization normalization NN cord-258014-lzzi4rnz 313 8 or or CC cord-258014-lzzi4rnz 313 9 excluded exclude VBN cord-258014-lzzi4rnz 313 10 from from IN cord-258014-lzzi4rnz 313 11 the the DT cord-258014-lzzi4rnz 313 12 analysis analysis NN cord-258014-lzzi4rnz 313 13 . . . cord-258014-lzzi4rnz 314 1 In in IN cord-258014-lzzi4rnz 314 2 many many JJ cord-258014-lzzi4rnz 314 3 cases case NNS cord-258014-lzzi4rnz 314 4 , , , cord-258014-lzzi4rnz 314 5 outliers outlier NNS cord-258014-lzzi4rnz 314 6 are be VBP cord-258014-lzzi4rnz 314 7 the the DT cord-258014-lzzi4rnz 314 8 result result NN cord-258014-lzzi4rnz 314 9 of of IN cord-258014-lzzi4rnz 314 10 operational operational JJ cord-258014-lzzi4rnz 314 11 errors error NNS cord-258014-lzzi4rnz 314 12 during during IN cord-258014-lzzi4rnz 314 13 the the DT cord-258014-lzzi4rnz 314 14 analytical analytical JJ cord-258014-lzzi4rnz 314 15 process process NN cord-258014-lzzi4rnz 314 16 . . . cord-258014-lzzi4rnz 315 1 If if IN cord-258014-lzzi4rnz 315 2 those those DT cord-258014-lzzi4rnz 315 3 values value NNS cord-258014-lzzi4rnz 315 4 can can MD cord-258014-lzzi4rnz 315 5 not not RB cord-258014-lzzi4rnz 315 6 be be VB cord-258014-lzzi4rnz 315 7 corrected correct VBN cord-258014-lzzi4rnz 315 8 , , , cord-258014-lzzi4rnz 315 9 they -PRON- PRP cord-258014-lzzi4rnz 315 10 should should MD cord-258014-lzzi4rnz 315 11 be be VB cord-258014-lzzi4rnz 315 12 removed remove VBN cord-258014-lzzi4rnz 315 13 from from IN cord-258014-lzzi4rnz 315 14 the the DT cord-258014-lzzi4rnz 315 15 analysis analysis NN cord-258014-lzzi4rnz 315 16 , , , cord-258014-lzzi4rnz 315 17 but but CC cord-258014-lzzi4rnz 315 18 always always RB cord-258014-lzzi4rnz 315 19 justified justified JJ cord-258014-lzzi4rnz 315 20 [ [ -LRB- cord-258014-lzzi4rnz 315 21 39 39 CD cord-258014-lzzi4rnz 315 22 ] ] -RRB- cord-258014-lzzi4rnz 315 23 . . . cord-258014-lzzi4rnz 316 1 Please please UH cord-258014-lzzi4rnz 316 2 refer refer VB cord-258014-lzzi4rnz 316 3 to to IN cord-258014-lzzi4rnz 316 4 MetaboAnalyst.ca metaboanalyst.ca JJ cord-258014-lzzi4rnz 316 5 tutorials tutorial NNS cord-258014-lzzi4rnz 316 6 , , , cord-258014-lzzi4rnz 316 7 or or CC cord-258014-lzzi4rnz 316 8 FAQ FAQ NNP cord-258014-lzzi4rnz 316 9 to to TO cord-258014-lzzi4rnz 316 10 select select VB cord-258014-lzzi4rnz 316 11 the the DT cord-258014-lzzi4rnz 316 12 statistical statistical JJ cord-258014-lzzi4rnz 316 13 analysis analysis NN cord-258014-lzzi4rnz 316 14 method method NN cord-258014-lzzi4rnz 316 15 . . . cord-258014-lzzi4rnz 317 1 17 17 CD cord-258014-lzzi4rnz 317 2 . . . cord-258014-lzzi4rnz 318 1 Well well RB cord-258014-lzzi4rnz 318 2 - - HYPH cord-258014-lzzi4rnz 318 3 predicted predict VBN cord-258014-lzzi4rnz 318 4 metabolites metabolite NNS cord-258014-lzzi4rnz 318 5 were be VBD cord-258014-lzzi4rnz 318 6 identified identify VBN cord-258014-lzzi4rnz 318 7 by by IN cord-258014-lzzi4rnz 318 8 the the DT cord-258014-lzzi4rnz 318 9 Community Community NNP cord-258014-lzzi4rnz 318 10 Metabolic Metabolic NNP cord-258014-lzzi4rnz 318 11 Potential Potential NNP cord-258014-lzzi4rnz 318 12 ( ( -LRB- cord-258014-lzzi4rnz 318 13 CMP CMP NNP cord-258014-lzzi4rnz 318 14 ) ) -RRB- cord-258014-lzzi4rnz 318 15 scores score NNS cord-258014-lzzi4rnz 318 16 model model VBP cord-258014-lzzi4rnz 318 17 in in IN cord-258014-lzzi4rnz 318 18 groups group NNS cord-258014-lzzi4rnz 318 19 by by IN cord-258014-lzzi4rnz 318 20 examining examine VBG cord-258014-lzzi4rnz 318 21 of of IN cord-258014-lzzi4rnz 318 22 the the DT cord-258014-lzzi4rnz 318 23 total total JJ cord-258014-lzzi4rnz 318 24 pool pool NN cord-258014-lzzi4rnz 318 25 of of IN cord-258014-lzzi4rnz 318 26 metabolites metabolite NNS cord-258014-lzzi4rnz 318 27 with with IN cord-258014-lzzi4rnz 318 28 positive positive JJ cord-258014-lzzi4rnz 318 29 model model NN cord-258014-lzzi4rnz 318 30 slope slope NN cord-258014-lzzi4rnz 318 31 and and CC cord-258014-lzzi4rnz 318 32 a a DT cord-258014-lzzi4rnz 318 33 model model NN cord-258014-lzzi4rnz 318 34 p p NN cord-258014-lzzi4rnz 318 35 - - HYPH cord-258014-lzzi4rnz 318 36 value value NN cord-258014-lzzi4rnz 318 37 < < XX cord-258014-lzzi4rnz 318 38 0.1 0.1 CD cord-258014-lzzi4rnz 318 39 . . . cord-258014-lzzi4rnz 319 1 Given give VBN cord-258014-lzzi4rnz 319 2 that that IN cord-258014-lzzi4rnz 319 3 MIMOSA2 MIMOSA2 NNS cord-258014-lzzi4rnz 319 4 is be VBZ cord-258014-lzzi4rnz 319 5 prone prone JJ cord-258014-lzzi4rnz 319 6 to to IN cord-258014-lzzi4rnz 319 7 false false JJ cord-258014-lzzi4rnz 319 8 negatives negative NNS cord-258014-lzzi4rnz 319 9 because because IN cord-258014-lzzi4rnz 319 10 its -PRON- PRP$ cord-258014-lzzi4rnz 319 11 model model NN cord-258014-lzzi4rnz 319 12 is be VBZ cord-258014-lzzi4rnz 319 13 approximate approximate JJ cord-258014-lzzi4rnz 319 14 , , , cord-258014-lzzi4rnz 319 15 it -PRON- PRP cord-258014-lzzi4rnz 319 16 requires require VBZ cord-258014-lzzi4rnz 319 17 a a DT cord-258014-lzzi4rnz 319 18 weak weak JJ cord-258014-lzzi4rnz 319 19 statistical statistical JJ cord-258014-lzzi4rnz 319 20 threshold threshold NN cord-258014-lzzi4rnz 319 21 ( ( -LRB- cord-258014-lzzi4rnz 319 22 p p NN cord-258014-lzzi4rnz 319 23 - - HYPH cord-258014-lzzi4rnz 319 24 value value NN cord-258014-lzzi4rnz 319 25 < < XX cord-258014-lzzi4rnz 319 26 0.1 0.1 CD cord-258014-lzzi4rnz 319 27 ) ) -RRB- cord-258014-lzzi4rnz 319 28 to to TO cord-258014-lzzi4rnz 319 29 capture capture VB cord-258014-lzzi4rnz 319 30 more more JJR cord-258014-lzzi4rnz 319 31 relationships relationship NNS cord-258014-lzzi4rnz 319 32 of of IN cord-258014-lzzi4rnz 319 33 possible possible JJ cord-258014-lzzi4rnz 319 34 interest interest NN cord-258014-lzzi4rnz 319 35 [ [ -LRB- cord-258014-lzzi4rnz 319 36 18 18 CD cord-258014-lzzi4rnz 319 37 , , , cord-258014-lzzi4rnz 319 38 41 41 CD cord-258014-lzzi4rnz 319 39 , , , cord-258014-lzzi4rnz 319 40 42 42 CD cord-258014-lzzi4rnz 319 41 ] ] -RRB- cord-258014-lzzi4rnz 319 42 . . . cord-258014-lzzi4rnz 320 1 Additional additional JJ cord-258014-lzzi4rnz 320 2 information information NN cord-258014-lzzi4rnz 320 3 could could MD cord-258014-lzzi4rnz 320 4 be be VB cord-258014-lzzi4rnz 320 5 found find VBN cord-258014-lzzi4rnz 320 6 at at IN cord-258014-lzzi4rnz 320 7 borensteinlab.com/software_MIMOSA2.html borensteinlab.com/software_mimosa2.html NN cord-258014-lzzi4rnz 320 8 helper helper NN cord-258014-lzzi4rnz 320 9 . . . cord-258014-lzzi4rnz 321 1 We -PRON- PRP cord-258014-lzzi4rnz 321 2 are be VBP cord-258014-lzzi4rnz 321 3 assisting assist VBG cord-258014-lzzi4rnz 321 4 a a DT cord-258014-lzzi4rnz 321 5 new new JJ cord-258014-lzzi4rnz 321 6 stage stage NN cord-258014-lzzi4rnz 321 7 of of IN cord-258014-lzzi4rnz 321 8 microbiome microbiome NN cord-258014-lzzi4rnz 321 9 research research NN cord-258014-lzzi4rnz 321 10 , , , cord-258014-lzzi4rnz 321 11 which which WDT cord-258014-lzzi4rnz 321 12 moves move VBZ cord-258014-lzzi4rnz 321 13 beyond beyond IN cord-258014-lzzi4rnz 321 14 the the DT cord-258014-lzzi4rnz 321 15 typical typical JJ cord-258014-lzzi4rnz 321 16 16S 16s JJ cord-258014-lzzi4rnz 321 17 profiles profile NNS cord-258014-lzzi4rnz 321 18 listing list VBG cord-258014-lzzi4rnz 321 19 bacteria bacteria NNS cord-258014-lzzi4rnz 321 20 associated associate VBN cord-258014-lzzi4rnz 321 21 to to IN cord-258014-lzzi4rnz 321 22 a a DT cord-258014-lzzi4rnz 321 23 given give VBN cord-258014-lzzi4rnz 321 24 body body NN cord-258014-lzzi4rnz 321 25 site site NN cord-258014-lzzi4rnz 321 26 or or CC cord-258014-lzzi4rnz 321 27 disease disease NN cord-258014-lzzi4rnz 321 28 , , , cord-258014-lzzi4rnz 321 29 and and CC cord-258014-lzzi4rnz 321 30 offers offer VBZ cord-258014-lzzi4rnz 321 31 the the DT cord-258014-lzzi4rnz 321 32 novel novel JJ cord-258014-lzzi4rnz 321 33 integration integration NN cord-258014-lzzi4rnz 321 34 of of IN cord-258014-lzzi4rnz 321 35 other other JJ cord-258014-lzzi4rnz 321 36 omics omic NNS cord-258014-lzzi4rnz 321 37 approaches approach NNS cord-258014-lzzi4rnz 321 38 , , , cord-258014-lzzi4rnz 321 39 towards towards IN cord-258014-lzzi4rnz 321 40 a a DT cord-258014-lzzi4rnz 321 41 better well JJR cord-258014-lzzi4rnz 321 42 understanding understanding NN cord-258014-lzzi4rnz 321 43 of of IN cord-258014-lzzi4rnz 321 44 community community NN cord-258014-lzzi4rnz 321 45 functions function NNS cord-258014-lzzi4rnz 321 46 in in IN cord-258014-lzzi4rnz 321 47 the the DT cord-258014-lzzi4rnz 321 48 disease disease NN cord-258014-lzzi4rnz 321 49 , , , cord-258014-lzzi4rnz 321 50 and and CC cord-258014-lzzi4rnz 321 51 interactions interaction NNS cord-258014-lzzi4rnz 321 52 with with IN cord-258014-lzzi4rnz 321 53 the the DT cord-258014-lzzi4rnz 321 54 host host NN cord-258014-lzzi4rnz 321 55 . . . cord-258014-lzzi4rnz 322 1 Emphasis emphasis NN cord-258014-lzzi4rnz 322 2 is be VBZ cord-258014-lzzi4rnz 322 3 now now RB cord-258014-lzzi4rnz 322 4 given give VBN cord-258014-lzzi4rnz 322 5 to to IN cord-258014-lzzi4rnz 322 6 the the DT cord-258014-lzzi4rnz 322 7 causality causality NN cord-258014-lzzi4rnz 322 8 of of IN cord-258014-lzzi4rnz 322 9 microbiome microbiome NN cord-258014-lzzi4rnz 322 10 research research NN cord-258014-lzzi4rnz 322 11 and and CC cord-258014-lzzi4rnz 322 12 the the DT cord-258014-lzzi4rnz 322 13 development development NN cord-258014-lzzi4rnz 322 14 of of IN cord-258014-lzzi4rnz 322 15 mechanistic mechanistic JJ cord-258014-lzzi4rnz 322 16 understandings understanding NNS cord-258014-lzzi4rnz 322 17 of of IN cord-258014-lzzi4rnz 322 18 the the DT cord-258014-lzzi4rnz 322 19 influence influence NN cord-258014-lzzi4rnz 322 20 of of IN cord-258014-lzzi4rnz 322 21 microbiota microbiota NN cord-258014-lzzi4rnz 322 22 , , , cord-258014-lzzi4rnz 322 23 including include VBG cord-258014-lzzi4rnz 322 24 their -PRON- PRP$ cord-258014-lzzi4rnz 322 25 metabolic metabolic JJ cord-258014-lzzi4rnz 322 26 activities activity NNS cord-258014-lzzi4rnz 322 27 . . . cord-258014-lzzi4rnz 323 1 Thus thus RB cord-258014-lzzi4rnz 323 2 , , , cord-258014-lzzi4rnz 323 3 the the DT cord-258014-lzzi4rnz 323 4 integration integration NN cord-258014-lzzi4rnz 323 5 of of IN cord-258014-lzzi4rnz 323 6 metagenomics metagenomic NNS cord-258014-lzzi4rnz 323 7 and and CC cord-258014-lzzi4rnz 323 8 metabolomics metabolomic NNS cord-258014-lzzi4rnz 323 9 is be VBZ cord-258014-lzzi4rnz 323 10 becoming become VBG cord-258014-lzzi4rnz 323 11 a a DT cord-258014-lzzi4rnz 323 12 very very RB cord-258014-lzzi4rnz 323 13 promising promising JJ cord-258014-lzzi4rnz 323 14 approach approach NN cord-258014-lzzi4rnz 323 15 in in IN cord-258014-lzzi4rnz 323 16 the the DT cord-258014-lzzi4rnz 323 17 exploration exploration NN cord-258014-lzzi4rnz 323 18 of of IN cord-258014-lzzi4rnz 323 19 associations association NNS cord-258014-lzzi4rnz 323 20 between between IN cord-258014-lzzi4rnz 323 21 the the DT cord-258014-lzzi4rnz 323 22 host host NN cord-258014-lzzi4rnz 323 23 microbiome microbiome NN cord-258014-lzzi4rnz 323 24 and and CC cord-258014-lzzi4rnz 323 25 circulating circulate VBG cord-258014-lzzi4rnz 323 26 metabolites metabolite NNS cord-258014-lzzi4rnz 323 27 with with IN cord-258014-lzzi4rnz 323 28 the the DT cord-258014-lzzi4rnz 323 29 aim aim NN cord-258014-lzzi4rnz 323 30 of of IN cord-258014-lzzi4rnz 323 31 understanding understand VBG cord-258014-lzzi4rnz 323 32 the the DT cord-258014-lzzi4rnz 323 33 complex complex JJ cord-258014-lzzi4rnz 323 34 bacterial bacterial JJ cord-258014-lzzi4rnz 323 35 interactions interaction NNS cord-258014-lzzi4rnz 323 36 with with IN cord-258014-lzzi4rnz 323 37 the the DT cord-258014-lzzi4rnz 323 38 host host NN cord-258014-lzzi4rnz 323 39 and and CC cord-258014-lzzi4rnz 323 40 their -PRON- PRP$ cord-258014-lzzi4rnz 323 41 contributions contribution NNS cord-258014-lzzi4rnz 323 42 to to IN cord-258014-lzzi4rnz 323 43 systemic systemic JJ cord-258014-lzzi4rnz 323 44 health health NN cord-258014-lzzi4rnz 323 45 . . . cord-258014-lzzi4rnz 324 1 Using use VBG cord-258014-lzzi4rnz 324 2 urine urine NN cord-258014-lzzi4rnz 324 3 as as IN cord-258014-lzzi4rnz 324 4 a a DT cord-258014-lzzi4rnz 324 5 liquid liquid JJ cord-258014-lzzi4rnz 324 6 biopsy biopsy NN cord-258014-lzzi4rnz 324 7 is be VBZ cord-258014-lzzi4rnz 324 8 gaining gain VBG cord-258014-lzzi4rnz 324 9 more more JJR cord-258014-lzzi4rnz 324 10 popularity popularity NN cord-258014-lzzi4rnz 324 11 . . . cord-258014-lzzi4rnz 325 1 Indeed indeed RB cord-258014-lzzi4rnz 325 2 , , , cord-258014-lzzi4rnz 325 3 evidence evidence NN cord-258014-lzzi4rnz 325 4 suggests suggest VBZ cord-258014-lzzi4rnz 325 5 that that IN cord-258014-lzzi4rnz 325 6 biomarkers biomarker NNS cord-258014-lzzi4rnz 325 7 for for IN cord-258014-lzzi4rnz 325 8 cervical cervical JJ cord-258014-lzzi4rnz 325 9 cancer cancer NN cord-258014-lzzi4rnz 325 10 may may MD cord-258014-lzzi4rnz 325 11 be be VB cord-258014-lzzi4rnz 325 12 exfoliated exfoliate VBN cord-258014-lzzi4rnz 325 13 as as IN cord-258014-lzzi4rnz 325 14 debris debris NN cord-258014-lzzi4rnz 325 15 in in IN cord-258014-lzzi4rnz 325 16 urine urine NN cord-258014-lzzi4rnz 325 17 , , , cord-258014-lzzi4rnz 325 18 which which WDT cord-258014-lzzi4rnz 325 19 could could MD cord-258014-lzzi4rnz 325 20 facilitate facilitate VB cord-258014-lzzi4rnz 325 21 diagnostics diagnostic NNS cord-258014-lzzi4rnz 325 22 [ [ -LRB- cord-258014-lzzi4rnz 325 23 50 50 CD cord-258014-lzzi4rnz 325 24 ] ] -RRB- cord-258014-lzzi4rnz 325 25 . . . cord-258014-lzzi4rnz 326 1 Since since IN cord-258014-lzzi4rnz 326 2 circulating circulate VBG cord-258014-lzzi4rnz 326 3 metabolites metabolite NNS cord-258014-lzzi4rnz 326 4 are be VBP cord-258014-lzzi4rnz 326 5 finally finally RB cord-258014-lzzi4rnz 326 6 excreted excrete VBN cord-258014-lzzi4rnz 326 7 through through IN cord-258014-lzzi4rnz 326 8 urine urine NN cord-258014-lzzi4rnz 326 9 [ [ -LRB- cord-258014-lzzi4rnz 326 10 51 51 CD cord-258014-lzzi4rnz 326 11 ] ] -RRB- cord-258014-lzzi4rnz 326 12 , , , cord-258014-lzzi4rnz 326 13 recent recent JJ cord-258014-lzzi4rnz 326 14 studies study NNS cord-258014-lzzi4rnz 326 15 suggest suggest VBP cord-258014-lzzi4rnz 326 16 that that IN cord-258014-lzzi4rnz 326 17 exploring explore VBG cord-258014-lzzi4rnz 326 18 the the DT cord-258014-lzzi4rnz 326 19 association association NN cord-258014-lzzi4rnz 326 20 between between IN cord-258014-lzzi4rnz 326 21 urine urine NN cord-258014-lzzi4rnz 326 22 metabolome metabolome NNP cord-258014-lzzi4rnz 326 23 and and CC cord-258014-lzzi4rnz 326 24 host host NN cord-258014-lzzi4rnz 326 25 microbiome microbiome NN cord-258014-lzzi4rnz 326 26 ( ( -LRB- cord-258014-lzzi4rnz 326 27 gut gut VB cord-258014-lzzi4rnz 326 28 or or CC cord-258014-lzzi4rnz 326 29 even even RB cord-258014-lzzi4rnz 326 30 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 326 31 ) ) -RRB- cord-258014-lzzi4rnz 326 32 may may MD cord-258014-lzzi4rnz 326 33 complement complement VB cord-258014-lzzi4rnz 326 34 sequencing sequencing NN cord-258014-lzzi4rnz 326 35 - - HYPH cord-258014-lzzi4rnz 326 36 based base VBN cord-258014-lzzi4rnz 326 37 approaches approach NNS cord-258014-lzzi4rnz 326 38 with with IN cord-258014-lzzi4rnz 326 39 a a DT cord-258014-lzzi4rnz 326 40 functional functional JJ cord-258014-lzzi4rnz 326 41 readout readout NN cord-258014-lzzi4rnz 326 42 of of IN cord-258014-lzzi4rnz 326 43 the the DT cord-258014-lzzi4rnz 326 44 host host NN cord-258014-lzzi4rnz 326 45 microbiome microbiome NN cord-258014-lzzi4rnz 326 46 and and CC cord-258014-lzzi4rnz 326 47 its -PRON- PRP$ cord-258014-lzzi4rnz 326 48 interaction interaction NN cord-258014-lzzi4rnz 326 49 with with IN cord-258014-lzzi4rnz 326 50 host host NN cord-258014-lzzi4rnz 326 51 [ [ -LRB- cord-258014-lzzi4rnz 326 52 52 52 CD cord-258014-lzzi4rnz 326 53 , , , cord-258014-lzzi4rnz 326 54 53 53 CD cord-258014-lzzi4rnz 326 55 ] ] -RRB- cord-258014-lzzi4rnz 326 56 . . . cord-258014-lzzi4rnz 327 1 Indeed indeed RB cord-258014-lzzi4rnz 327 2 , , , cord-258014-lzzi4rnz 327 3 a a DT cord-258014-lzzi4rnz 327 4 recent recent JJ cord-258014-lzzi4rnz 327 5 study study NN cord-258014-lzzi4rnz 327 6 coupling couple VBG cord-258014-lzzi4rnz 327 7 gut gut NN cord-258014-lzzi4rnz 327 8 microbiome microbiome NN cord-258014-lzzi4rnz 327 9 and and CC cord-258014-lzzi4rnz 327 10 urine urine NN cord-258014-lzzi4rnz 327 11 metabolomics metabolomic NNS cord-258014-lzzi4rnz 327 12 suggests suggest VBZ cord-258014-lzzi4rnz 327 13 that that IN cord-258014-lzzi4rnz 327 14 gut gut VB cord-258014-lzzi4rnz 327 15 bacterial bacterial JJ cord-258014-lzzi4rnz 327 16 compositional compositional JJ cord-258014-lzzi4rnz 327 17 changes change NNS cord-258014-lzzi4rnz 327 18 could could MD cord-258014-lzzi4rnz 327 19 eventually eventually RB cord-258014-lzzi4rnz 327 20 be be VB cord-258014-lzzi4rnz 327 21 monitored monitor VBN cord-258014-lzzi4rnz 327 22 and and CC cord-258014-lzzi4rnz 327 23 probed probe VBN cord-258014-lzzi4rnz 327 24 using use VBG cord-258014-lzzi4rnz 327 25 urine urine NN cord-258014-lzzi4rnz 327 26 metabolomics metabolomic NNS cord-258014-lzzi4rnz 327 27 [ [ -LRB- cord-258014-lzzi4rnz 327 28 52 52 CD cord-258014-lzzi4rnz 327 29 ] ] -RRB- cord-258014-lzzi4rnz 327 30 . . . cord-258014-lzzi4rnz 328 1 In in IN cord-258014-lzzi4rnz 328 2 addition addition NN cord-258014-lzzi4rnz 328 3 , , , cord-258014-lzzi4rnz 328 4 the the DT cord-258014-lzzi4rnz 328 5 application application NN cord-258014-lzzi4rnz 328 6 of of IN cord-258014-lzzi4rnz 328 7 the the DT cord-258014-lzzi4rnz 328 8 current current JJ cord-258014-lzzi4rnz 328 9 protocol protocol NN cord-258014-lzzi4rnz 328 10 for for IN cord-258014-lzzi4rnz 328 11 functional functional JJ cord-258014-lzzi4rnz 328 12 characterization characterization NN cord-258014-lzzi4rnz 328 13 of of IN cord-258014-lzzi4rnz 328 14 vaginal vaginal JJ cord-258014-lzzi4rnz 328 15 and and CC cord-258014-lzzi4rnz 328 16 cervical cervical JJ cord-258014-lzzi4rnz 328 17 hr hr NN cord-258014-lzzi4rnz 328 18 HPV HPV NNP cord-258014-lzzi4rnz 328 19 microbiota microbiota NN cord-258014-lzzi4rnz 328 20 and and CC cord-258014-lzzi4rnz 328 21 urinary urinary JJ cord-258014-lzzi4rnz 328 22 metabolome metabolome NNP cord-258014-lzzi4rnz 328 23 has have VBZ cord-258014-lzzi4rnz 328 24 identified identify VBN cord-258014-lzzi4rnz 328 25 species specie NNS cord-258014-lzzi4rnz 328 26 - - : cord-258014-lzzi4rnz 328 27 contributors contributor NNS cord-258014-lzzi4rnz 328 28 to to IN cord-258014-lzzi4rnz 328 29 the the DT cord-258014-lzzi4rnz 328 30 pool pool NN cord-258014-lzzi4rnz 328 31 of of IN cord-258014-lzzi4rnz 328 32 several several JJ cord-258014-lzzi4rnz 328 33 circulating circulate VBG cord-258014-lzzi4rnz 328 34 urinary urinary JJ cord-258014-lzzi4rnz 328 35 metabolites metabolite NNS cord-258014-lzzi4rnz 328 36 associated associate VBN cord-258014-lzzi4rnz 328 37 with with IN cord-258014-lzzi4rnz 328 38 cervical cervical JJ cord-258014-lzzi4rnz 328 39 cancer cancer NN cord-258014-lzzi4rnz 328 40 development development NN cord-258014-lzzi4rnz 328 41 [ [ -LRB- cord-258014-lzzi4rnz 328 42 18 18 CD cord-258014-lzzi4rnz 328 43 ] ] -RRB- cord-258014-lzzi4rnz 328 44 . . . cord-258014-lzzi4rnz 329 1 The the DT cord-258014-lzzi4rnz 329 2 high high JJ cord-258014-lzzi4rnz 329 3 throughput throughput NN cord-258014-lzzi4rnz 329 4 analyses analysis NNS cord-258014-lzzi4rnz 329 5 of of IN cord-258014-lzzi4rnz 329 6 these these DT cord-258014-lzzi4rnz 329 7 two two CD cord-258014-lzzi4rnz 329 8 datasets dataset NNS cord-258014-lzzi4rnz 329 9 could could MD cord-258014-lzzi4rnz 329 10 be be VB cord-258014-lzzi4rnz 329 11 key key JJ cord-258014-lzzi4rnz 329 12 for for IN cord-258014-lzzi4rnz 329 13 the the DT cord-258014-lzzi4rnz 329 14 development development NN cord-258014-lzzi4rnz 329 15 of of IN cord-258014-lzzi4rnz 329 16 new new JJ cord-258014-lzzi4rnz 329 17 tools tool NNS cord-258014-lzzi4rnz 329 18 ( ( -LRB- cord-258014-lzzi4rnz 329 19 like like IN cord-258014-lzzi4rnz 329 20 portable portable JJ cord-258014-lzzi4rnz 329 21 devices device NNS cord-258014-lzzi4rnz 329 22 ) ) -RRB- cord-258014-lzzi4rnz 329 23 to to TO cord-258014-lzzi4rnz 329 24 give give VB cord-258014-lzzi4rnz 329 25 women woman NNS cord-258014-lzzi4rnz 329 26 feasible feasible JJ cord-258014-lzzi4rnz 329 27 self self NN cord-258014-lzzi4rnz 329 28 - - HYPH cord-258014-lzzi4rnz 329 29 testing testing NN cord-258014-lzzi4rnz 329 30 , , , cord-258014-lzzi4rnz 329 31 and and CC cord-258014-lzzi4rnz 329 32 to to TO cord-258014-lzzi4rnz 329 33 allow allow VB cord-258014-lzzi4rnz 329 34 them -PRON- PRP cord-258014-lzzi4rnz 329 35 to to TO cord-258014-lzzi4rnz 329 36 be be VB cord-258014-lzzi4rnz 329 37 informed inform VBN cord-258014-lzzi4rnz 329 38 of of IN cord-258014-lzzi4rnz 329 39 their -PRON- PRP$ cord-258014-lzzi4rnz 329 40 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 329 41 dysbiosis dysbiosis NN cord-258014-lzzi4rnz 329 42 via via IN cord-258014-lzzi4rnz 329 43 vaginal vaginal JJ cord-258014-lzzi4rnz 329 44 or or CC cord-258014-lzzi4rnz 329 45 urine urine NN cord-258014-lzzi4rnz 329 46 metabolomic metabolomic JJ cord-258014-lzzi4rnz 329 47 screening screening NN cord-258014-lzzi4rnz 329 48 , , , cord-258014-lzzi4rnz 329 49 which which WDT cord-258014-lzzi4rnz 329 50 could could MD cord-258014-lzzi4rnz 329 51 benefit benefit VB cord-258014-lzzi4rnz 329 52 low low JJ cord-258014-lzzi4rnz 329 53 - - HYPH cord-258014-lzzi4rnz 329 54 income income NN cord-258014-lzzi4rnz 329 55 countries country NNS cord-258014-lzzi4rnz 329 56 , , , cord-258014-lzzi4rnz 329 57 and and CC cord-258014-lzzi4rnz 329 58 those those DT cord-258014-lzzi4rnz 329 59 with with IN cord-258014-lzzi4rnz 329 60 a a DT cord-258014-lzzi4rnz 329 61 lack lack NN cord-258014-lzzi4rnz 329 62 of of IN cord-258014-lzzi4rnz 329 63 access access NN cord-258014-lzzi4rnz 329 64 to to IN cord-258014-lzzi4rnz 329 65 cervical cervical JJ cord-258014-lzzi4rnz 329 66 surveillance surveillance NN cord-258014-lzzi4rnz 329 67 . . . cord-258014-lzzi4rnz 330 1 Taken take VBN cord-258014-lzzi4rnz 330 2 together together RB cord-258014-lzzi4rnz 330 3 , , , cord-258014-lzzi4rnz 330 4 this this DT cord-258014-lzzi4rnz 330 5 report report NN cord-258014-lzzi4rnz 330 6 is be VBZ cord-258014-lzzi4rnz 330 7 a a DT cord-258014-lzzi4rnz 330 8 simple simple JJ cord-258014-lzzi4rnz 330 9 and and CC cord-258014-lzzi4rnz 330 10 easy easy JJ cord-258014-lzzi4rnz 330 11 - - : cord-258014-lzzi4rnz 330 12 to to TO cord-258014-lzzi4rnz 330 13 follow follow VB cord-258014-lzzi4rnz 330 14 protocol protocol NN cord-258014-lzzi4rnz 330 15 , , , cord-258014-lzzi4rnz 330 16 aimed aim VBN cord-258014-lzzi4rnz 330 17 to to TO cord-258014-lzzi4rnz 330 18 be be VB cord-258014-lzzi4rnz 330 19 used use VBN cord-258014-lzzi4rnz 330 20 as as IN cord-258014-lzzi4rnz 330 21 a a DT cord-258014-lzzi4rnz 330 22 guide guide NN cord-258014-lzzi4rnz 330 23 for for IN cord-258014-lzzi4rnz 330 24 those those DT cord-258014-lzzi4rnz 330 25 taking take VBG cord-258014-lzzi4rnz 330 26 on on RP cord-258014-lzzi4rnz 330 27 microbiota microbiota NN cord-258014-lzzi4rnz 330 28 and and CC cord-258014-lzzi4rnz 330 29 metabolic metabolic JJ cord-258014-lzzi4rnz 330 30 analyses analysis NNS cord-258014-lzzi4rnz 330 31 using use VBG cord-258014-lzzi4rnz 330 32 human human JJ cord-258014-lzzi4rnz 330 33 biospecimens biospecimen NNS cord-258014-lzzi4rnz 330 34 , , , cord-258014-lzzi4rnz 330 35 such such JJ cord-258014-lzzi4rnz 330 36 as as IN cord-258014-lzzi4rnz 330 37 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 330 38 microbiota microbiota NN cord-258014-lzzi4rnz 330 39 and and CC cord-258014-lzzi4rnz 330 40 urine urine NN cord-258014-lzzi4rnz 330 41 metabolomics metabolomic NNS cord-258014-lzzi4rnz 330 42 [ [ -LRB- cord-258014-lzzi4rnz 330 43 5 5 CD cord-258014-lzzi4rnz 330 44 , , , cord-258014-lzzi4rnz 330 45 18 18 CD cord-258014-lzzi4rnz 330 46 , , , cord-258014-lzzi4rnz 330 47 52 52 CD cord-258014-lzzi4rnz 330 48 ] ] -RRB- cord-258014-lzzi4rnz 330 49 . . . cord-258014-lzzi4rnz 331 1 Data data NN cord-258014-lzzi4rnz 331 2 integration integration NN cord-258014-lzzi4rnz 331 3 via via IN cord-258014-lzzi4rnz 331 4 the the DT cord-258014-lzzi4rnz 331 5 use use NN cord-258014-lzzi4rnz 331 6 of of IN cord-258014-lzzi4rnz 331 7 freely freely RB cord-258014-lzzi4rnz 331 8 available available JJ cord-258014-lzzi4rnz 331 9 MIMOSA2 MIMOSA2 NNS cord-258014-lzzi4rnz 331 10 software software NN cord-258014-lzzi4rnz 331 11 [ [ -LRB- cord-258014-lzzi4rnz 331 12 42 42 CD cord-258014-lzzi4rnz 331 13 ] ] -RRB- cord-258014-lzzi4rnz 331 14 facilitates facilitate VBZ cord-258014-lzzi4rnz 331 15 analyses analysis NNS cord-258014-lzzi4rnz 331 16 providing provide VBG cord-258014-lzzi4rnz 331 17 easy easy JJ cord-258014-lzzi4rnz 331 18 export export NN cord-258014-lzzi4rnz 331 19 files file NNS cord-258014-lzzi4rnz 331 20 that that WDT cord-258014-lzzi4rnz 331 21 can can MD cord-258014-lzzi4rnz 331 22 be be VB cord-258014-lzzi4rnz 331 23 plotted plot VBN cord-258014-lzzi4rnz 331 24 in in IN cord-258014-lzzi4rnz 331 25 any any DT cord-258014-lzzi4rnz 331 26 other other JJ cord-258014-lzzi4rnz 331 27 widely widely RB cord-258014-lzzi4rnz 331 28 used use VBN cord-258014-lzzi4rnz 331 29 packages package NNS cord-258014-lzzi4rnz 331 30 such such JJ cord-258014-lzzi4rnz 331 31 as as IN cord-258014-lzzi4rnz 331 32 R R NNP cord-258014-lzzi4rnz 331 33 [ [ -LRB- cord-258014-lzzi4rnz 331 34 54 54 CD cord-258014-lzzi4rnz 331 35 ] ] -RRB- cord-258014-lzzi4rnz 331 36 . . . cord-258014-lzzi4rnz 332 1 Generating generate VBG cord-258014-lzzi4rnz 332 2 data datum NNS cord-258014-lzzi4rnz 332 3 that that WDT cord-258014-lzzi4rnz 332 4 combines combine VBZ cord-258014-lzzi4rnz 332 5 both both CC cord-258014-lzzi4rnz 332 6 the the DT cord-258014-lzzi4rnz 332 7 microbiome microbiome NN cord-258014-lzzi4rnz 332 8 and and CC cord-258014-lzzi4rnz 332 9 metabolomics metabolomics NN cord-258014-lzzi4rnz 332 10 analyses analysis NNS cord-258014-lzzi4rnz 332 11 will will MD cord-258014-lzzi4rnz 332 12 guide guide VB cord-258014-lzzi4rnz 332 13 the the DT cord-258014-lzzi4rnz 332 14 development development NN cord-258014-lzzi4rnz 332 15 of of IN cord-258014-lzzi4rnz 332 16 tools tool NNS cord-258014-lzzi4rnz 332 17 aimed aim VBN cord-258014-lzzi4rnz 332 18 at at IN cord-258014-lzzi4rnz 332 19 detecting detect VBG cord-258014-lzzi4rnz 332 20 dysbiosis dysbiosis NN cord-258014-lzzi4rnz 332 21 via via IN cord-258014-lzzi4rnz 332 22 changes change NNS cord-258014-lzzi4rnz 332 23 of of IN cord-258014-lzzi4rnz 332 24 the the DT cord-258014-lzzi4rnz 332 25 microbial microbial JJ cord-258014-lzzi4rnz 332 26 profiles profile NNS cord-258014-lzzi4rnz 332 27 and and CC cord-258014-lzzi4rnz 332 28 metabolomic metabolomic JJ cord-258014-lzzi4rnz 332 29 signatures signature NNS cord-258014-lzzi4rnz 332 30 , , , cord-258014-lzzi4rnz 332 31 which which WDT cord-258014-lzzi4rnz 332 32 will will MD cord-258014-lzzi4rnz 332 33 be be VB cord-258014-lzzi4rnz 332 34 useful useful JJ cord-258014-lzzi4rnz 332 35 for for IN cord-258014-lzzi4rnz 332 36 new new JJ cord-258014-lzzi4rnz 332 37 point point NN cord-258014-lzzi4rnz 332 38 - - HYPH cord-258014-lzzi4rnz 332 39 of of IN cord-258014-lzzi4rnz 332 40 - - HYPH cord-258014-lzzi4rnz 332 41 care care NN cord-258014-lzzi4rnz 332 42 diagnostics diagnostic NNS cord-258014-lzzi4rnz 332 43 . . . cord-258014-lzzi4rnz 332 44 _SP cord-258014-lzzi4rnz 333 1 The the DT cord-258014-lzzi4rnz 333 2 authors author NNS cord-258014-lzzi4rnz 333 3 declare declare VBP cord-258014-lzzi4rnz 333 4 no no DT cord-258014-lzzi4rnz 333 5 conflict conflict NN cord-258014-lzzi4rnz 333 6 of of IN cord-258014-lzzi4rnz 333 7 interest interest NN cord-258014-lzzi4rnz 333 8 . . . cord-258014-lzzi4rnz 334 1 An an DT cord-258014-lzzi4rnz 334 2 ecological ecological JJ cord-258014-lzzi4rnz 334 3 and and CC cord-258014-lzzi4rnz 334 4 evolutionary evolutionary JJ cord-258014-lzzi4rnz 334 5 perspective perspective NN cord-258014-lzzi4rnz 334 6 on on IN cord-258014-lzzi4rnz 334 7 human human NN cord-258014-lzzi4rnz 334 8 - - HYPH cord-258014-lzzi4rnz 334 9 microbe microbe NN cord-258014-lzzi4rnz 334 10 mutualism mutualism NN cord-258014-lzzi4rnz 334 11 and and CC cord-258014-lzzi4rnz 334 12 disease disease NN cord-258014-lzzi4rnz 334 13 Human human JJ cord-258014-lzzi4rnz 334 14 microbial microbial JJ cord-258014-lzzi4rnz 334 15 ecology ecology NN cord-258014-lzzi4rnz 334 16 and and CC cord-258014-lzzi4rnz 334 17 the the DT cord-258014-lzzi4rnz 334 18 rising rise VBG cord-258014-lzzi4rnz 334 19 new new JJ cord-258014-lzzi4rnz 334 20 medicine medicine NN cord-258014-lzzi4rnz 334 21 Role role NN cord-258014-lzzi4rnz 334 22 of of IN cord-258014-lzzi4rnz 334 23 the the DT cord-258014-lzzi4rnz 334 24 microbiome microbiome NN cord-258014-lzzi4rnz 334 25 in in IN cord-258014-lzzi4rnz 334 26 human human JJ cord-258014-lzzi4rnz 334 27 development development NN cord-258014-lzzi4rnz 334 28 Vaginal vaginal JJ cord-258014-lzzi4rnz 334 29 microbiome microbiome NN cord-258014-lzzi4rnz 334 30 of of IN cord-258014-lzzi4rnz 334 31 reproductive reproductive JJ cord-258014-lzzi4rnz 334 32 - - HYPH cord-258014-lzzi4rnz 334 33 age age NN cord-258014-lzzi4rnz 334 34 women woman NNS cord-258014-lzzi4rnz 334 35 Cervicovaginal Cervicovaginal NNP cord-258014-lzzi4rnz 334 36 Fungi Fungi NNPS cord-258014-lzzi4rnz 334 37 and and CC cord-258014-lzzi4rnz 334 38 Bacteria Bacteria NNS cord-258014-lzzi4rnz 335 1 Associated associate VBN cord-258014-lzzi4rnz 335 2 With with IN cord-258014-lzzi4rnz 335 3 Cervical cervical JJ cord-258014-lzzi4rnz 335 4 Intraepithelial Intraepithelial NNP cord-258014-lzzi4rnz 335 5 Neoplasia Neoplasia NNP cord-258014-lzzi4rnz 335 6 and and CC cord-258014-lzzi4rnz 335 7 High High NNP cord-258014-lzzi4rnz 335 8 - - HYPH cord-258014-lzzi4rnz 335 9 Risk risk NN cord-258014-lzzi4rnz 335 10 Human Human NNP cord-258014-lzzi4rnz 335 11 Papillomavirus Papillomavirus NNP cord-258014-lzzi4rnz 335 12 Infections Infections NNPS cord-258014-lzzi4rnz 335 13 in in IN cord-258014-lzzi4rnz 335 14 a a DT cord-258014-lzzi4rnz 335 15 Hispanic hispanic JJ cord-258014-lzzi4rnz 335 16 Population population NN cord-258014-lzzi4rnz 336 1 The the DT cord-258014-lzzi4rnz 336 2 cervical cervical JJ cord-258014-lzzi4rnz 336 3 microbiome microbiome NN cord-258014-lzzi4rnz 336 4 over over IN cord-258014-lzzi4rnz 336 5 7 7 CD cord-258014-lzzi4rnz 336 6 years year NNS cord-258014-lzzi4rnz 336 7 and and CC cord-258014-lzzi4rnz 336 8 a a DT cord-258014-lzzi4rnz 336 9 comparison comparison NN cord-258014-lzzi4rnz 336 10 of of IN cord-258014-lzzi4rnz 336 11 methodologies methodology NNS cord-258014-lzzi4rnz 336 12 for for IN cord-258014-lzzi4rnz 336 13 its -PRON- PRP$ cord-258014-lzzi4rnz 336 14 characterization characterization NN cord-258014-lzzi4rnz 336 15 Cervical Cervical NNP cord-258014-lzzi4rnz 336 16 Microbiome Microbiome NNP cord-258014-lzzi4rnz 336 17 and and CC cord-258014-lzzi4rnz 336 18 Cytokine Cytokine NNP cord-258014-lzzi4rnz 336 19 Profile Profile NNP cord-258014-lzzi4rnz 336 20 at at IN cord-258014-lzzi4rnz 336 21 Various various JJ cord-258014-lzzi4rnz 336 22 Stages stage NNS cord-258014-lzzi4rnz 336 23 of of IN cord-258014-lzzi4rnz 336 24 Cervical Cervical NNP cord-258014-lzzi4rnz 336 25 Cancer Cancer NNP cord-258014-lzzi4rnz 336 26 : : : cord-258014-lzzi4rnz 337 1 A a DT cord-258014-lzzi4rnz 337 2 Pilot Pilot NNP cord-258014-lzzi4rnz 337 3 Study study NN cord-258014-lzzi4rnz 338 1 The the DT cord-258014-lzzi4rnz 338 2 effect effect NN cord-258014-lzzi4rnz 338 3 of of IN cord-258014-lzzi4rnz 338 4 vaginal vaginal JJ cord-258014-lzzi4rnz 338 5 douching douching NN cord-258014-lzzi4rnz 338 6 cessation cessation NN cord-258014-lzzi4rnz 338 7 on on IN cord-258014-lzzi4rnz 338 8 bacterial bacterial JJ cord-258014-lzzi4rnz 338 9 vaginosis vaginosis NN cord-258014-lzzi4rnz 338 10 : : : cord-258014-lzzi4rnz 338 11 A a DT cord-258014-lzzi4rnz 338 12 pilot pilot NN cord-258014-lzzi4rnz 338 13 study study NN cord-258014-lzzi4rnz 338 14 Diversity diversity NN cord-258014-lzzi4rnz 338 15 of of IN cord-258014-lzzi4rnz 338 16 human human JJ cord-258014-lzzi4rnz 338 17 vaginal vaginal JJ cord-258014-lzzi4rnz 338 18 bacterial bacterial JJ cord-258014-lzzi4rnz 338 19 communities community NNS cord-258014-lzzi4rnz 338 20 and and CC cord-258014-lzzi4rnz 338 21 associations association NNS cord-258014-lzzi4rnz 338 22 with with IN cord-258014-lzzi4rnz 338 23 clinically clinically RB cord-258014-lzzi4rnz 338 24 defined define VBN cord-258014-lzzi4rnz 338 25 bacterial bacterial JJ cord-258014-lzzi4rnz 338 26 vaginosis vaginosis NN cord-258014-lzzi4rnz 338 27 An an DT cord-258014-lzzi4rnz 338 28 association association NN cord-258014-lzzi4rnz 338 29 of of IN cord-258014-lzzi4rnz 338 30 cervical cervical JJ cord-258014-lzzi4rnz 338 31 inflammation inflammation NN cord-258014-lzzi4rnz 338 32 with with IN cord-258014-lzzi4rnz 338 33 high high JJ cord-258014-lzzi4rnz 338 34 - - HYPH cord-258014-lzzi4rnz 338 35 grade grade NN cord-258014-lzzi4rnz 338 36 cervical cervical JJ cord-258014-lzzi4rnz 338 37 neoplasia neoplasia NN cord-258014-lzzi4rnz 338 38 in in IN cord-258014-lzzi4rnz 338 39 women woman NNS cord-258014-lzzi4rnz 338 40 infected infect VBN cord-258014-lzzi4rnz 338 41 with with IN cord-258014-lzzi4rnz 338 42 oncogenic oncogenic JJ cord-258014-lzzi4rnz 338 43 human human JJ cord-258014-lzzi4rnz 338 44 papillomavirus papillomavirus NN cord-258014-lzzi4rnz 338 45 ( ( -LRB- cord-258014-lzzi4rnz 338 46 HPV HPV NNP cord-258014-lzzi4rnz 338 47 ) ) -RRB- cord-258014-lzzi4rnz 338 48 Deciphering decipher VBG cord-258014-lzzi4rnz 338 49 the the DT cord-258014-lzzi4rnz 338 50 complex complex JJ cord-258014-lzzi4rnz 338 51 interplay interplay NN cord-258014-lzzi4rnz 338 52 between between IN cord-258014-lzzi4rnz 338 53 microbiota microbiota NN cord-258014-lzzi4rnz 338 54 , , , cord-258014-lzzi4rnz 338 55 HPV hpv NN cord-258014-lzzi4rnz 338 56 , , , cord-258014-lzzi4rnz 338 57 inflammation inflammation NN cord-258014-lzzi4rnz 338 58 and and CC cord-258014-lzzi4rnz 338 59 cancer cancer NN cord-258014-lzzi4rnz 338 60 through through IN cord-258014-lzzi4rnz 338 61 cervicovaginal cervicovaginal JJ cord-258014-lzzi4rnz 338 62 metabolic metabolic JJ cord-258014-lzzi4rnz 338 63 profiling profiling NN cord-258014-lzzi4rnz 339 1 The the DT cord-258014-lzzi4rnz 339 2 impact impact NN cord-258014-lzzi4rnz 339 3 of of IN cord-258014-lzzi4rnz 339 4 regular regular JJ cord-258014-lzzi4rnz 339 5 vaginal vaginal JJ cord-258014-lzzi4rnz 339 6 pH ph NN cord-258014-lzzi4rnz 339 7 screening screening NN cord-258014-lzzi4rnz 339 8 on on IN cord-258014-lzzi4rnz 339 9 the the DT cord-258014-lzzi4rnz 339 10 diagnosis diagnosis NN cord-258014-lzzi4rnz 339 11 of of IN cord-258014-lzzi4rnz 339 12 bacterial bacterial JJ cord-258014-lzzi4rnz 339 13 vaginosis vaginosis NN cord-258014-lzzi4rnz 339 14 in in IN cord-258014-lzzi4rnz 339 15 pregnancy pregnancy NN cord-258014-lzzi4rnz 339 16 Vaginal vaginal JJ cord-258014-lzzi4rnz 339 17 fluid fluid NN cord-258014-lzzi4rnz 339 18 pH ph NN cord-258014-lzzi4rnz 339 19 , , , cord-258014-lzzi4rnz 339 20 cervicovaginitis cervicovaginitis NNP cord-258014-lzzi4rnz 339 21 and and CC cord-258014-lzzi4rnz 339 22 cervical cervical JJ cord-258014-lzzi4rnz 339 23 length length NN cord-258014-lzzi4rnz 339 24 in in IN cord-258014-lzzi4rnz 339 25 pregnancy pregnancy NN cord-258014-lzzi4rnz 340 1 The the DT cord-258014-lzzi4rnz 340 2 causal causal JJ cord-258014-lzzi4rnz 340 3 relation relation NN cord-258014-lzzi4rnz 340 4 between between IN cord-258014-lzzi4rnz 340 5 human human JJ cord-258014-lzzi4rnz 340 6 papillomavirus papillomavirus NN cord-258014-lzzi4rnz 340 7 and and CC cord-258014-lzzi4rnz 340 8 cervical cervical JJ cord-258014-lzzi4rnz 340 9 cancer cancer NN cord-258014-lzzi4rnz 340 10 Human Human NNP cord-258014-lzzi4rnz 340 11 papillomavirus papillomavirus NN cord-258014-lzzi4rnz 340 12 and and CC cord-258014-lzzi4rnz 340 13 oral oral JJ cord-258014-lzzi4rnz 340 14 cancer cancer NN cord-258014-lzzi4rnz 340 15 : : : cord-258014-lzzi4rnz 340 16 The the DT cord-258014-lzzi4rnz 340 17 International International NNP cord-258014-lzzi4rnz 340 18 Agency Agency NNP cord-258014-lzzi4rnz 340 19 for for IN cord-258014-lzzi4rnz 340 20 Research Research NNP cord-258014-lzzi4rnz 340 21 on on IN cord-258014-lzzi4rnz 340 22 Cancer Cancer NNP cord-258014-lzzi4rnz 340 23 multicenter multicenter JJ cord-258014-lzzi4rnz 340 24 study study NN cord-258014-lzzi4rnz 341 1 The the DT cord-258014-lzzi4rnz 341 2 microbiota microbiota NN cord-258014-lzzi4rnz 341 3 associated associate VBN cord-258014-lzzi4rnz 341 4 to to IN cord-258014-lzzi4rnz 341 5 cervical cervical JJ cord-258014-lzzi4rnz 341 6 and and CC cord-258014-lzzi4rnz 341 7 anal anal JJ cord-258014-lzzi4rnz 341 8 HPV HPV NNP cord-258014-lzzi4rnz 341 9 infections infection NNS cord-258014-lzzi4rnz 341 10 in in IN cord-258014-lzzi4rnz 341 11 a a DT cord-258014-lzzi4rnz 341 12 Hispanic hispanic JJ cord-258014-lzzi4rnz 341 13 population population NN cord-258014-lzzi4rnz 341 14 Variations variation NNS cord-258014-lzzi4rnz 341 15 in in IN cord-258014-lzzi4rnz 341 16 gut gut NN cord-258014-lzzi4rnz 341 17 microbiota microbiota NNS cord-258014-lzzi4rnz 341 18 and and CC cord-258014-lzzi4rnz 341 19 fecal fecal JJ cord-258014-lzzi4rnz 341 20 metabolic metabolic JJ cord-258014-lzzi4rnz 341 21 phenotype phenotype NN cord-258014-lzzi4rnz 341 22 associated associate VBN cord-258014-lzzi4rnz 341 23 with with IN cord-258014-lzzi4rnz 341 24 depression depression NN cord-258014-lzzi4rnz 341 25 by by IN cord-258014-lzzi4rnz 341 26 16S 16S NNP cord-258014-lzzi4rnz 341 27 rRNA rRNA NNP cord-258014-lzzi4rnz 341 28 gene gene NN cord-258014-lzzi4rnz 341 29 sequencing sequencing NN cord-258014-lzzi4rnz 341 30 and and CC cord-258014-lzzi4rnz 341 31 LC LC NNP cord-258014-lzzi4rnz 341 32 / / SYM cord-258014-lzzi4rnz 341 33 MS MS NNP cord-258014-lzzi4rnz 341 34 - - HYPH cord-258014-lzzi4rnz 341 35 based base VBN cord-258014-lzzi4rnz 341 36 metabolomics metabolomic NNS cord-258014-lzzi4rnz 341 37 Cervicovaginal Cervicovaginal NNP cord-258014-lzzi4rnz 341 38 Microbiome Microbiome NNP cord-258014-lzzi4rnz 341 39 and and CC cord-258014-lzzi4rnz 341 40 Urine Urine NNP cord-258014-lzzi4rnz 341 41 Metabolome Metabolome NNP cord-258014-lzzi4rnz 341 42 Paired Paired NNP cord-258014-lzzi4rnz 341 43 Analysis Analysis NNP cord-258014-lzzi4rnz 341 44 Reveals reveal VBZ cord-258014-lzzi4rnz 341 45 Niche Niche NNP cord-258014-lzzi4rnz 341 46 Partitioning Partitioning NNP cord-258014-lzzi4rnz 341 47 of of IN cord-258014-lzzi4rnz 341 48 the the DT cord-258014-lzzi4rnz 341 49 Microbiota Microbiota NNP cord-258014-lzzi4rnz 341 50 in in IN cord-258014-lzzi4rnz 341 51 Patients Patients NNPS cord-258014-lzzi4rnz 341 52 with with IN cord-258014-lzzi4rnz 341 53 Human Human NNP cord-258014-lzzi4rnz 341 54 Papilloma Papilloma NNP cord-258014-lzzi4rnz 341 55 Virus Virus NNP cord-258014-lzzi4rnz 341 56 Infections Infections NNP cord-258014-lzzi4rnz 341 57 Identification Identification NNP cord-258014-lzzi4rnz 341 58 of of IN cord-258014-lzzi4rnz 341 59 a a DT cord-258014-lzzi4rnz 341 60 novel novel JJ cord-258014-lzzi4rnz 341 61 human human JJ cord-258014-lzzi4rnz 341 62 papillomavirus papillomavirus NN cord-258014-lzzi4rnz 341 63 by by IN cord-258014-lzzi4rnz 341 64 metagenomic metagenomic NNP cord-258014-lzzi4rnz 341 65 analysis analysis NN cord-258014-lzzi4rnz 341 66 of of IN cord-258014-lzzi4rnz 341 67 vaginal vaginal JJ cord-258014-lzzi4rnz 341 68 swab swab NN cord-258014-lzzi4rnz 341 69 samples sample NNS cord-258014-lzzi4rnz 341 70 from from IN cord-258014-lzzi4rnz 341 71 pregnant pregnant JJ cord-258014-lzzi4rnz 341 72 women woman NNS cord-258014-lzzi4rnz 341 73 Advantages Advantages NNPS cord-258014-lzzi4rnz 341 74 of of IN cord-258014-lzzi4rnz 341 75 meta meta NNP cord-258014-lzzi4rnz 341 76 - - HYPH cord-258014-lzzi4rnz 341 77 total total JJ cord-258014-lzzi4rnz 341 78 RNA RNA NNP cord-258014-lzzi4rnz 341 79 sequencing sequencing NN cord-258014-lzzi4rnz 341 80 ( ( -LRB- cord-258014-lzzi4rnz 341 81 MeTRS MeTRS NNP cord-258014-lzzi4rnz 341 82 ) ) -RRB- cord-258014-lzzi4rnz 341 83 over over IN cord-258014-lzzi4rnz 341 84 shotgun shotgun NN cord-258014-lzzi4rnz 341 85 metagenomics metagenomic NNS cord-258014-lzzi4rnz 341 86 and and CC cord-258014-lzzi4rnz 341 87 amplicon amplicon NN cord-258014-lzzi4rnz 341 88 - - HYPH cord-258014-lzzi4rnz 341 89 based base VBN cord-258014-lzzi4rnz 341 90 sequencing sequencing NN cord-258014-lzzi4rnz 341 91 in in IN cord-258014-lzzi4rnz 341 92 the the DT cord-258014-lzzi4rnz 341 93 profiling profiling NN cord-258014-lzzi4rnz 341 94 of of IN cord-258014-lzzi4rnz 341 95 complex complex JJ cord-258014-lzzi4rnz 341 96 microbial microbial JJ cord-258014-lzzi4rnz 341 97 communities community NNS cord-258014-lzzi4rnz 341 98 Nanowire Nanowire NNP cord-258014-lzzi4rnz 341 99 sensors sensor NNS cord-258014-lzzi4rnz 341 100 monitor monitor VBP cord-258014-lzzi4rnz 341 101 bacterial bacterial JJ cord-258014-lzzi4rnz 341 102 growth growth NN cord-258014-lzzi4rnz 341 103 kinetics kinetic NNS cord-258014-lzzi4rnz 341 104 and and CC cord-258014-lzzi4rnz 341 105 response response NN cord-258014-lzzi4rnz 341 106 to to IN cord-258014-lzzi4rnz 341 107 antibiotics antibiotic NNS cord-258014-lzzi4rnz 341 108 Development Development NNP cord-258014-lzzi4rnz 341 109 and and CC cord-258014-lzzi4rnz 341 110 use use NN cord-258014-lzzi4rnz 341 111 of of IN cord-258014-lzzi4rnz 341 112 fluorescent fluorescent JJ cord-258014-lzzi4rnz 341 113 nanosensors nanosensor NNS cord-258014-lzzi4rnz 341 114 for for IN cord-258014-lzzi4rnz 341 115 metabolite metabolite NN cord-258014-lzzi4rnz 341 116 imaging imaging NN cord-258014-lzzi4rnz 341 117 in in IN cord-258014-lzzi4rnz 341 118 living living NN cord-258014-lzzi4rnz 341 119 cells cell NNS cord-258014-lzzi4rnz 341 120 Manual Manual NNP cord-258014-lzzi4rnz 341 121 of of IN cord-258014-lzzi4rnz 341 122 Procedures Procedures NNPS cord-258014-lzzi4rnz 341 123 - - : cord-258014-lzzi4rnz 341 124 Human Human NNP cord-258014-lzzi4rnz 341 125 Microbiome Microbiome NNP cord-258014-lzzi4rnz 341 126 Project Project NNP cord-258014-lzzi4rnz 341 127 : : : cord-258014-lzzi4rnz 341 128 Core Core NNP cord-258014-lzzi4rnz 341 129 Microbiome Microbiome NNP cord-258014-lzzi4rnz 341 130 Sampling Sampling NNP cord-258014-lzzi4rnz 341 131 , , , cord-258014-lzzi4rnz 341 132 Protocol Protocol NNP cord-258014-lzzi4rnz 341 133 A A NNP cord-258014-lzzi4rnz 341 134 , , , cord-258014-lzzi4rnz 341 135 HMP HMP NNP cord-258014-lzzi4rnz 341 136 Protocol Protocol NNP cord-258014-lzzi4rnz 341 137 # # NN cord-258014-lzzi4rnz 341 138 07 07 CD cord-258014-lzzi4rnz 341 139 - - HYPH cord-258014-lzzi4rnz 341 140 001 001 CD cord-258014-lzzi4rnz 341 141 Development Development NNP cord-258014-lzzi4rnz 341 142 and and CC cord-258014-lzzi4rnz 341 143 clinical clinical JJ cord-258014-lzzi4rnz 341 144 evaluation evaluation NN cord-258014-lzzi4rnz 341 145 of of IN cord-258014-lzzi4rnz 341 146 a a DT cord-258014-lzzi4rnz 341 147 highly highly RB cord-258014-lzzi4rnz 341 148 sensitive sensitive JJ cord-258014-lzzi4rnz 341 149 PCR pcr NN cord-258014-lzzi4rnz 341 150 - - HYPH cord-258014-lzzi4rnz 341 151 reverse reverse JJ cord-258014-lzzi4rnz 341 152 hybridization hybridization NN cord-258014-lzzi4rnz 341 153 line line NN cord-258014-lzzi4rnz 341 154 probe probe NN cord-258014-lzzi4rnz 341 155 assay assay NN cord-258014-lzzi4rnz 341 156 for for IN cord-258014-lzzi4rnz 341 157 detection detection NN cord-258014-lzzi4rnz 341 158 and and CC cord-258014-lzzi4rnz 341 159 identification identification NN cord-258014-lzzi4rnz 341 160 of of IN cord-258014-lzzi4rnz 341 161 anogenital anogenital JJ cord-258014-lzzi4rnz 341 162 human human JJ cord-258014-lzzi4rnz 341 163 papillomavirus papillomavirus NN cord-258014-lzzi4rnz 341 164 Reactivation Reactivation NNP cord-258014-lzzi4rnz 341 165 of of IN cord-258014-lzzi4rnz 341 166 Latent Latent NNP cord-258014-lzzi4rnz 341 167 HPV HPV NNP cord-258014-lzzi4rnz 341 168 Infections infection NNS cord-258014-lzzi4rnz 342 1 After after IN cord-258014-lzzi4rnz 342 2 Renal Renal NNP cord-258014-lzzi4rnz 342 3 Transplantation Transplantation NNP cord-258014-lzzi4rnz 342 4 High high JJ cord-258014-lzzi4rnz 342 5 Rate rate NN cord-258014-lzzi4rnz 342 6 of of IN cord-258014-lzzi4rnz 342 7 Infection infection NN cord-258014-lzzi4rnz 342 8 by by IN cord-258014-lzzi4rnz 342 9 Only only JJ cord-258014-lzzi4rnz 342 10 Oncogenic oncogenic JJ cord-258014-lzzi4rnz 342 11 Human Human NNP cord-258014-lzzi4rnz 342 12 Papillomavirus Papillomavirus NNP cord-258014-lzzi4rnz 342 13 in in IN cord-258014-lzzi4rnz 342 14 Amerindians Amerindians NNP cord-258014-lzzi4rnz 342 15 Ultra ultra JJ cord-258014-lzzi4rnz 342 16 - - JJ cord-258014-lzzi4rnz 342 17 high high JJ cord-258014-lzzi4rnz 342 18 - - HYPH cord-258014-lzzi4rnz 342 19 throughput throughput NN cord-258014-lzzi4rnz 342 20 microbial microbial JJ cord-258014-lzzi4rnz 342 21 community community NN cord-258014-lzzi4rnz 342 22 analysis analysis NN cord-258014-lzzi4rnz 342 23 on on IN cord-258014-lzzi4rnz 343 1 the the DT cord-258014-lzzi4rnz 343 2 Illumina Illumina NNP cord-258014-lzzi4rnz 343 3 HiSeq HiSeq NNP cord-258014-lzzi4rnz 343 4 and and CC cord-258014-lzzi4rnz 343 5 MiSeq MiSeq NNP cord-258014-lzzi4rnz 343 6 platforms platform NNS cord-258014-lzzi4rnz 343 7 Qiita Qiita NNP cord-258014-lzzi4rnz 343 8 : : : cord-258014-lzzi4rnz 344 1 Rapid Rapid NNP cord-258014-lzzi4rnz 344 2 , , , cord-258014-lzzi4rnz 344 3 web web NN cord-258014-lzzi4rnz 344 4 - - HYPH cord-258014-lzzi4rnz 344 5 enabled enable VBN cord-258014-lzzi4rnz 344 6 microbiome microbiome NN cord-258014-lzzi4rnz 344 7 meta meta NNP cord-258014-lzzi4rnz 344 8 - - HYPH cord-258014-lzzi4rnz 344 9 analysis analysis NNP cord-258014-lzzi4rnz 344 10 phyloseq phyloseq NN cord-258014-lzzi4rnz 344 11 : : : cord-258014-lzzi4rnz 345 1 An an DT cord-258014-lzzi4rnz 345 2 R r NN cord-258014-lzzi4rnz 345 3 package package NN cord-258014-lzzi4rnz 345 4 for for IN cord-258014-lzzi4rnz 345 5 reproducible reproducible JJ cord-258014-lzzi4rnz 345 6 interactive interactive JJ cord-258014-lzzi4rnz 345 7 analysis analysis NN cord-258014-lzzi4rnz 345 8 and and CC cord-258014-lzzi4rnz 345 9 graphics graphic NNS cord-258014-lzzi4rnz 345 10 of of IN cord-258014-lzzi4rnz 345 11 microbiome microbiome NNP cord-258014-lzzi4rnz 345 12 census census NNP cord-258014-lzzi4rnz 345 13 data datum NNS cord-258014-lzzi4rnz 345 14 MicrobiomeAnalyst MicrobiomeAnalyst NNP cord-258014-lzzi4rnz 345 15 : : : cord-258014-lzzi4rnz 346 1 A a DT cord-258014-lzzi4rnz 346 2 web web NN cord-258014-lzzi4rnz 346 3 - - HYPH cord-258014-lzzi4rnz 346 4 based base VBN cord-258014-lzzi4rnz 346 5 tool tool NN cord-258014-lzzi4rnz 346 6 for for IN cord-258014-lzzi4rnz 346 7 comprehensive comprehensive JJ cord-258014-lzzi4rnz 346 8 statistical statistical JJ cord-258014-lzzi4rnz 346 9 , , , cord-258014-lzzi4rnz 346 10 visual visual JJ cord-258014-lzzi4rnz 346 11 and and CC cord-258014-lzzi4rnz 346 12 meta meta JJ cord-258014-lzzi4rnz 346 13 - - HYPH cord-258014-lzzi4rnz 346 14 analysis analysis NN cord-258014-lzzi4rnz 346 15 of of IN cord-258014-lzzi4rnz 346 16 microbiome microbiome NN cord-258014-lzzi4rnz 346 17 data datum NNS cord-258014-lzzi4rnz 347 1 The the DT cord-258014-lzzi4rnz 347 2 Mathematical Mathematical NNP cord-258014-lzzi4rnz 347 3 Theory Theory NNP cord-258014-lzzi4rnz 347 4 of of IN cord-258014-lzzi4rnz 347 5 Communication Communication NNP cord-258014-lzzi4rnz 347 6 Elegant Elegant NNP cord-258014-lzzi4rnz 347 7 Graphics Graphics NNPS cord-258014-lzzi4rnz 347 8 for for IN cord-258014-lzzi4rnz 347 9 Data Data NNP cord-258014-lzzi4rnz 347 10 Analysis Analysis NNP cord-258014-lzzi4rnz 347 11 Pretty Pretty NNP cord-258014-lzzi4rnz 347 12 Heatmaps Heatmaps NNP cord-258014-lzzi4rnz 347 13 Metagenomic Metagenomic NNP cord-258014-lzzi4rnz 347 14 biomarker biomarker NN cord-258014-lzzi4rnz 347 15 discovery discovery NN cord-258014-lzzi4rnz 347 16 and and CC cord-258014-lzzi4rnz 347 17 explanation explanation NN cord-258014-lzzi4rnz 347 18 R r NN cord-258014-lzzi4rnz 347 19 : : : cord-258014-lzzi4rnz 347 20 A a DT cord-258014-lzzi4rnz 347 21 Language Language NNP cord-258014-lzzi4rnz 347 22 and and CC cord-258014-lzzi4rnz 347 23 Environment Environment NNP cord-258014-lzzi4rnz 347 24 for for IN cord-258014-lzzi4rnz 347 25 Statistical Statistical NNP cord-258014-lzzi4rnz 347 26 Computing Computing NNP cord-258014-lzzi4rnz 347 27 ; ; : cord-258014-lzzi4rnz 347 28 Foundation Foundation NNP cord-258014-lzzi4rnz 347 29 for for IN cord-258014-lzzi4rnz 347 30 statistical statistical JJ cord-258014-lzzi4rnz 347 31 computing computing NN cord-258014-lzzi4rnz 347 32 Community Community NNP cord-258014-lzzi4rnz 347 33 Ecology Ecology NNP cord-258014-lzzi4rnz 347 34 Package Package NNP cord-258014-lzzi4rnz 347 35 . . . cord-258014-lzzi4rnz 348 1 R R NNP cord-258014-lzzi4rnz 348 2 Package Package NNP cord-258014-lzzi4rnz 348 3 Version Version NNP cord-258014-lzzi4rnz 348 4 2.4 2.4 CD cord-258014-lzzi4rnz 348 5 - - SYM cord-258014-lzzi4rnz 348 6 4 4 CD cord-258014-lzzi4rnz 348 7 . . . cord-258014-lzzi4rnz 349 1 R R NNP cord-258014-lzzi4rnz 349 2 Development Development NNP cord-258014-lzzi4rnz 349 3 Core Core NNP cord-258014-lzzi4rnz 349 4 Team Team NNP cord-258014-lzzi4rnz 349 5 . . . cord-258014-lzzi4rnz 350 1 R r NN cord-258014-lzzi4rnz 350 2 : : : cord-258014-lzzi4rnz 351 1 A a DT cord-258014-lzzi4rnz 351 2 Language Language NNP cord-258014-lzzi4rnz 351 3 and and CC cord-258014-lzzi4rnz 351 4 Environment Environment NNP cord-258014-lzzi4rnz 351 5 for for IN cord-258014-lzzi4rnz 351 6 Statistical Statistical NNP cord-258014-lzzi4rnz 351 7 Computing Computing NNP cord-258014-lzzi4rnz 351 8 ; ; : cord-258014-lzzi4rnz 351 9 R R NNP cord-258014-lzzi4rnz 351 10 Foundation Foundation NNP cord-258014-lzzi4rnz 351 11 for for IN cord-258014-lzzi4rnz 351 12 Statistical Statistical NNP cord-258014-lzzi4rnz 351 13 Computing Computing NNP cord-258014-lzzi4rnz 352 1 UniFrac UniFrac NNP cord-258014-lzzi4rnz 352 2 - - : cord-258014-lzzi4rnz 352 3 An an DT cord-258014-lzzi4rnz 352 4 online online JJ cord-258014-lzzi4rnz 352 5 tool tool NN cord-258014-lzzi4rnz 352 6 for for IN cord-258014-lzzi4rnz 352 7 comparing compare VBG cord-258014-lzzi4rnz 352 8 microbial microbial JJ cord-258014-lzzi4rnz 352 9 community community NN cord-258014-lzzi4rnz 352 10 diversity diversity NN cord-258014-lzzi4rnz 352 11 in in IN cord-258014-lzzi4rnz 352 12 a a DT cord-258014-lzzi4rnz 352 13 phylogenetic phylogenetic JJ cord-258014-lzzi4rnz 352 14 context context NN cord-258014-lzzi4rnz 353 1 A a DT cord-258014-lzzi4rnz 353 2 new new JJ cord-258014-lzzi4rnz 353 3 method method NN cord-258014-lzzi4rnz 353 4 for for IN cord-258014-lzzi4rnz 353 5 non non JJ cord-258014-lzzi4rnz 353 6 - - JJ cord-258014-lzzi4rnz 353 7 parametric parametric JJ cord-258014-lzzi4rnz 353 8 multivariate multivariate JJ cord-258014-lzzi4rnz 353 9 analysis analysis NN cord-258014-lzzi4rnz 353 10 of of IN cord-258014-lzzi4rnz 353 11 variance variance NN cord-258014-lzzi4rnz 353 12 MetaboAnalyst MetaboAnalyst NNP cord-258014-lzzi4rnz 353 13 3.0-Making 3.0-making CD cord-258014-lzzi4rnz 353 14 metabolomics metabolomic NNS cord-258014-lzzi4rnz 353 15 more more RBR cord-258014-lzzi4rnz 353 16 meaningful meaningful JJ cord-258014-lzzi4rnz 353 17 Web web NN cord-258014-lzzi4rnz 353 18 - - HYPH cord-258014-lzzi4rnz 353 19 based base VBN cord-258014-lzzi4rnz 353 20 inference inference NN cord-258014-lzzi4rnz 353 21 of of IN cord-258014-lzzi4rnz 353 22 biological biological JJ cord-258014-lzzi4rnz 353 23 patterns pattern NNS cord-258014-lzzi4rnz 353 24 , , , cord-258014-lzzi4rnz 353 25 functions function NNS cord-258014-lzzi4rnz 353 26 and and CC cord-258014-lzzi4rnz 353 27 pathways pathway NNS cord-258014-lzzi4rnz 353 28 from from IN cord-258014-lzzi4rnz 353 29 metabolomic metabolomic NNP cord-258014-lzzi4rnz 353 30 data datum NNS cord-258014-lzzi4rnz 353 31 using use VBG cord-258014-lzzi4rnz 353 32 MetaboAnalyst MetaboAnalyst NNP cord-258014-lzzi4rnz 353 33 Defining Defining NNP cord-258014-lzzi4rnz 353 34 and and CC cord-258014-lzzi4rnz 353 35 Evaluating evaluate VBG cord-258014-lzzi4rnz 353 36 Microbial Microbial NNP cord-258014-lzzi4rnz 353 37 Contributions Contributions NNPS cord-258014-lzzi4rnz 353 38 to to IN cord-258014-lzzi4rnz 353 39 Metabolite Metabolite NNP cord-258014-lzzi4rnz 353 40 Variation variation NN cord-258014-lzzi4rnz 353 41 in in IN cord-258014-lzzi4rnz 353 42 Microbiome Microbiome NNP cord-258014-lzzi4rnz 353 43 - - HYPH cord-258014-lzzi4rnz 353 44 Metabolome Metabolome NNP cord-258014-lzzi4rnz 353 45 Association Association NNP cord-258014-lzzi4rnz 353 46 Studies Studies NNP cord-258014-lzzi4rnz 353 47 Metabolic Metabolic NNP cord-258014-lzzi4rnz 353 48 Model Model NNP cord-258014-lzzi4rnz 353 49 - - HYPH cord-258014-lzzi4rnz 353 50 Based Based NNP cord-258014-lzzi4rnz 353 51 Integration Integration NNP cord-258014-lzzi4rnz 353 52 of of IN cord-258014-lzzi4rnz 353 53 Microbiome Microbiome NNP cord-258014-lzzi4rnz 353 54 Taxonomic Taxonomic NNP cord-258014-lzzi4rnz 353 55 and and CC cord-258014-lzzi4rnz 353 56 Metabolomic Metabolomic NNP cord-258014-lzzi4rnz 353 57 Profiles Profiles NNPS cord-258014-lzzi4rnz 353 58 Elucidates elucidate VBZ cord-258014-lzzi4rnz 353 59 Mechanistic mechanistic JJ cord-258014-lzzi4rnz 353 60 Links link NNS cord-258014-lzzi4rnz 353 61 between between IN cord-258014-lzzi4rnz 353 62 Ecological ecological JJ cord-258014-lzzi4rnz 353 63 and and CC cord-258014-lzzi4rnz 353 64 Metabolic metabolic JJ cord-258014-lzzi4rnz 353 65 Variation variation NN cord-258014-lzzi4rnz 353 66 . . . cord-258014-lzzi4rnz 354 1 mSystems mSystems NNP cord-258014-lzzi4rnz 354 2 Greengenes Greengenes NNP cord-258014-lzzi4rnz 354 3 , , , cord-258014-lzzi4rnz 354 4 a a DT cord-258014-lzzi4rnz 354 5 chimera chimera NN cord-258014-lzzi4rnz 354 6 - - HYPH cord-258014-lzzi4rnz 354 7 checked check VBN cord-258014-lzzi4rnz 354 8 16S 16S NNP cord-258014-lzzi4rnz 354 9 rRNA rRNA NNP cord-258014-lzzi4rnz 354 10 gene gene NN cord-258014-lzzi4rnz 354 11 database database NN cord-258014-lzzi4rnz 354 12 and and CC cord-258014-lzzi4rnz 354 13 workbench workbench NN cord-258014-lzzi4rnz 354 14 compatible compatible JJ cord-258014-lzzi4rnz 354 15 with with IN cord-258014-lzzi4rnz 354 16 ARB ARB NNP cord-258014-lzzi4rnz 354 17 Predictive Predictive NNP cord-258014-lzzi4rnz 354 18 functional functional JJ cord-258014-lzzi4rnz 354 19 profiling profiling NN cord-258014-lzzi4rnz 354 20 of of IN cord-258014-lzzi4rnz 354 21 microbial microbial JJ cord-258014-lzzi4rnz 354 22 communities community NNS cord-258014-lzzi4rnz 354 23 using use VBG cord-258014-lzzi4rnz 354 24 16S 16S NNP cord-258014-lzzi4rnz 354 25 rRNA rrna NN cord-258014-lzzi4rnz 354 26 marker marker NN cord-258014-lzzi4rnz 354 27 gene gene NN cord-258014-lzzi4rnz 354 28 sequences sequence NNS cord-258014-lzzi4rnz 355 1 Discussion discussion NN cord-258014-lzzi4rnz 355 2 101 101 CD cord-258014-lzzi4rnz 355 3 - - SYM cord-258014-lzzi4rnz 355 4 103 103 CD cord-258014-lzzi4rnz 355 5 Metabolomics Metabolomics NNPS cord-258014-lzzi4rnz 355 6 by by IN cord-258014-lzzi4rnz 355 7 Gas Gas NNP cord-258014-lzzi4rnz 355 8 Chromatography Chromatography NNP cord-258014-lzzi4rnz 355 9 - - HYPH cord-258014-lzzi4rnz 355 10 Mass Mass NNP cord-258014-lzzi4rnz 355 11 Spectrometry Spectrometry NNP cord-258014-lzzi4rnz 355 12 : : : cord-258014-lzzi4rnz 356 1 Combined Combined NNP cord-258014-lzzi4rnz 356 2 Targeted Targeted NNP cord-258014-lzzi4rnz 356 3 and and CC cord-258014-lzzi4rnz 356 4 Untargeted untargeted JJ cord-258014-lzzi4rnz 356 5 Profiling Profiling NNP cord-258014-lzzi4rnz 356 6 Metabolomics Metabolomics NNPS cord-258014-lzzi4rnz 356 7 Analysis Analysis NNP cord-258014-lzzi4rnz 356 8 of of IN cord-258014-lzzi4rnz 356 9 Glutamate Glutamate NNP cord-258014-lzzi4rnz 356 10 Receptor Receptor NNP cord-258014-lzzi4rnz 356 11 Function Function NNP cord-258014-lzzi4rnz 356 12 Centering Centering NNP cord-258014-lzzi4rnz 356 13 , , , cord-258014-lzzi4rnz 356 14 scaling scaling NN cord-258014-lzzi4rnz 356 15 , , , cord-258014-lzzi4rnz 356 16 and and CC cord-258014-lzzi4rnz 356 17 transformations transformation NNS cord-258014-lzzi4rnz 356 18 : : : cord-258014-lzzi4rnz 357 1 Improving improve VBG cord-258014-lzzi4rnz 357 2 the the DT cord-258014-lzzi4rnz 357 3 biological biological JJ cord-258014-lzzi4rnz 357 4 information information NN cord-258014-lzzi4rnz 357 5 content content NN cord-258014-lzzi4rnz 357 6 of of IN cord-258014-lzzi4rnz 357 7 metabolomics metabolomic NNS cord-258014-lzzi4rnz 357 8 data datum NNS cord-258014-lzzi4rnz 357 9 Standardizing Standardizing NNP cord-258014-lzzi4rnz 357 10 GC GC NNP cord-258014-lzzi4rnz 357 11 - - HYPH cord-258014-lzzi4rnz 357 12 MS MS NNP cord-258014-lzzi4rnz 357 13 metabolomics metabolomic VBZ cord-258014-lzzi4rnz 357 14 Urine urine NN cord-258014-lzzi4rnz 357 15 and and CC cord-258014-lzzi4rnz 357 16 bladder bladder NN cord-258014-lzzi4rnz 357 17 washing washing NN cord-258014-lzzi4rnz 357 18 cytology cytology NN cord-258014-lzzi4rnz 357 19 for for IN cord-258014-lzzi4rnz 357 20 detection detection NN cord-258014-lzzi4rnz 357 21 of of IN cord-258014-lzzi4rnz 357 22 urothelial urothelial JJ cord-258014-lzzi4rnz 357 23 carcinoma carcinoma NN cord-258014-lzzi4rnz 357 24 : : : cord-258014-lzzi4rnz 358 1 Standard standard JJ cord-258014-lzzi4rnz 358 2 test test NN cord-258014-lzzi4rnz 358 3 with with IN cord-258014-lzzi4rnz 358 4 new new JJ cord-258014-lzzi4rnz 358 5 possibilities possibility NNS cord-258014-lzzi4rnz 358 6 Uremic uremic JJ cord-258014-lzzi4rnz 358 7 solutes solute NNS cord-258014-lzzi4rnz 358 8 from from IN cord-258014-lzzi4rnz 358 9 colon colon NN cord-258014-lzzi4rnz 358 10 microbes microbe NNS cord-258014-lzzi4rnz 359 1 An an DT cord-258014-lzzi4rnz 359 2 untargeted untargeted JJ cord-258014-lzzi4rnz 359 3 fecal fecal NN cord-258014-lzzi4rnz 359 4 and and CC cord-258014-lzzi4rnz 359 5 urine urine NN cord-258014-lzzi4rnz 359 6 metabolomics metabolomic NNS cord-258014-lzzi4rnz 359 7 analysis analysis NN cord-258014-lzzi4rnz 359 8 of of IN cord-258014-lzzi4rnz 359 9 the the DT cord-258014-lzzi4rnz 359 10 interplay interplay NN cord-258014-lzzi4rnz 359 11 between between IN cord-258014-lzzi4rnz 359 12 the the DT cord-258014-lzzi4rnz 359 13 gut gut NN cord-258014-lzzi4rnz 359 14 microbiome microbiome NN cord-258014-lzzi4rnz 359 15 , , , cord-258014-lzzi4rnz 359 16 diet diet NN cord-258014-lzzi4rnz 359 17 and and CC cord-258014-lzzi4rnz 359 18 human human JJ cord-258014-lzzi4rnz 359 19 metabolism metabolism NN cord-258014-lzzi4rnz 359 20 in in IN cord-258014-lzzi4rnz 359 21 Indian indian JJ cord-258014-lzzi4rnz 359 22 and and CC cord-258014-lzzi4rnz 359 23 Chinese chinese JJ cord-258014-lzzi4rnz 359 24 adults adult NNS cord-258014-lzzi4rnz 359 25 Chapter Chapter NNP cord-258014-lzzi4rnz 359 26 8-Handing 8-Handing NNP cord-258014-lzzi4rnz 359 27 on on IN cord-258014-lzzi4rnz 359 28 Health Health NNP cord-258014-lzzi4rnz 359 29 to to IN cord-258014-lzzi4rnz 359 30 the the DT cord-258014-lzzi4rnz 359 31 Next next JJ cord-258014-lzzi4rnz 359 32 Generation generation NN cord-258014-lzzi4rnz 359 33 : : : cord-258014-lzzi4rnz 359 34 Early early JJ cord-258014-lzzi4rnz 359 35 Life Life NNP cord-258014-lzzi4rnz 359 36 Exposures exposure NNS cord-258014-lzzi4rnz 359 37 Discriminating discriminate VBG cord-258014-lzzi4rnz 359 38 high high JJ cord-258014-lzzi4rnz 359 39 - - HYPH cord-258014-lzzi4rnz 359 40 risk risk NN cord-258014-lzzi4rnz 359 41 cervical cervical JJ cord-258014-lzzi4rnz 359 42 Human Human NNP cord-258014-lzzi4rnz 359 43 Papilloma Papilloma NNP cord-258014-lzzi4rnz 359 44 Virus Virus NNP cord-258014-lzzi4rnz 359 45 infections infection NNS cord-258014-lzzi4rnz 359 46 with with IN cord-258014-lzzi4rnz 359 47 urinary urinary JJ cord-258014-lzzi4rnz 359 48 biomarkers biomarker NNS cord-258014-lzzi4rnz 359 49 via via IN cord-258014-lzzi4rnz 359 50 non non JJ cord-258014-lzzi4rnz 359 51 - - JJ cord-258014-lzzi4rnz 359 52 targeted targeted JJ cord-258014-lzzi4rnz 359 53 GC GC NNP cord-258014-lzzi4rnz 359 54 - - HYPH cord-258014-lzzi4rnz 359 55 MS MS NNP cord-258014-lzzi4rnz 359 56 - - HYPH cord-258014-lzzi4rnz 359 57 based base VBN cord-258014-lzzi4rnz 359 58 metabolomics metabolomic NNS cord-258014-lzzi4rnz 360 1 This this DT cord-258014-lzzi4rnz 360 2 article article NN cord-258014-lzzi4rnz 360 3 is be VBZ cord-258014-lzzi4rnz 360 4 an an DT cord-258014-lzzi4rnz 360 5 open open JJ cord-258014-lzzi4rnz 360 6 access access NN cord-258014-lzzi4rnz 360 7 article article NN cord-258014-lzzi4rnz 360 8 distributed distribute VBN cord-258014-lzzi4rnz 360 9 under under IN cord-258014-lzzi4rnz 360 10 the the DT cord-258014-lzzi4rnz 360 11 terms term NNS cord-258014-lzzi4rnz 360 12 and and CC cord-258014-lzzi4rnz 360 13 conditions condition NNS cord-258014-lzzi4rnz 360 14 of of IN cord-258014-lzzi4rnz 360 15 the the DT cord-258014-lzzi4rnz 360 16 Creative Creative NNP cord-258014-lzzi4rnz 360 17 Commons Commons NNP cord-258014-lzzi4rnz 360 18 Attribution Attribution NNP cord-258014-lzzi4rnz 360 19 ( ( -LRB- cord-258014-lzzi4rnz 360 20 CC CC NNP cord-258014-lzzi4rnz 360 21 BY by IN cord-258014-lzzi4rnz 360 22 ) ) -RRB- cord-258014-lzzi4rnz 360 23 license license NN