id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346906-1wmp43ti	Lewandowski, Kuiama	Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples	2019-12-23		.txt	text/plain	6766	328	43	We determine its sensitivity compared to that of existing diagnostic methods and its accuracy compared to short-read (Illumina) sequencing, using clinical samples from hospital patients during an influenza season and samples from a controlled laboratory infection in ferrets. During the study, respiratory samples submitted to the clinical diagnostic laboratory were routinely tested by a PCR-based test using the GeneXpert assay (Cepheid) to detect influenza A and B viruses and respiratory syncytial virus (RSV). Comparing this final method with our original protocol, using triplicate extractions from the pooled set of influenza A virus-positive samples demonstrated no significant loss in performance in the more rapid protocol (Fig. S3) , and we adopted this approach as our routine protocol, giving a wet-lab processing time of ϳ8 h. Future application of this method will involve real-time laboratory testing of respiratory samples, running the platform head to head with existing clinical diagnostics to further assess sensitivity and specificity, and using influenza virus sequence data to investigate transmission events.	./cache/cord-346906-1wmp43ti.txt	./txt/cord-346906-1wmp43ti.txt