id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-308687-wrzzb9cy	Brunner, Jesse L.	Pooled samples and eDNA-based detection can facilitate the “clean trade” of aquatic animals	2020-06-24		.txt	text/plain	6488	322	46	swabs) and eDNA samples collected from finite populations and discuss key assumptions and considerations for their use with a focus on detecting Batrachochytrium salamandrivorans, an emerging pathogen that threatens global salamander diversity. www.nature.com/scientificreports www.nature.com/scientificreports/ context of screening U.S. service members for syphilis, pooling samples to reduce the number of tests required to detect rare infections is common in numerous contexts 22 , including surveys for disease freedom. In this paper I therefore develop formulae for imperfect tests of pooled samples in closed populations and eDNA, discuss the key assumptions and considerations in their application, and illustrate how eDNA may be especially useful for detecting infections in the live animal trade. Pooling and batch-processing samples collected from individual animals (e.g., swabs) can reduce several fold the number of diagnostic tests (e.g., DNA extractions and PCR reactions) required to detect rare infections.	./cache/cord-308687-wrzzb9cy.txt	./txt/cord-308687-wrzzb9cy.txt