id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301823-fbeb1nw1	Sridhar, Sushmita	A blueprint for the implementation of a validated approach for the detection of SARS-Cov2 in clinical samples in academic facilities	2020-10-21		.txt	text/plain	6118	309	51	Here we describe our experience in establishing a COVID-19 diagnostics laboratory in an academic containment level 2 (CL2) research facility (UK) in which we validated and established a real-time PCR workflow to detect SARS-CoV2 in nose and throat swabs from HCWs. We developed an assay and workflow over eight working days (set-up to validation to screening) that can produce a quantitative diagnostic result ~4 hours after swabbing. Establishing and validating the workflow in our setting Establishing a workflow for SARS-Cov2 qRT-PCR Upon the decision to rapidly establish the qRT-PCR assay we identified several challenges, and these included: a) establishment and validation of a method suitable for diagnostic reporting, b) safe extraction of nucleic acid from a highly transmissible virus, c) accessing reagents required for performing extractions and amplifications, d) establishing a "clean" diagnostic workflow to minimise the risk of contamination, and e) creating a system in which HCWs could be swabbed and the data reported confidentially within a specified timeframe.	./cache/cord-301823-fbeb1nw1.txt	./txt/cord-301823-fbeb1nw1.txt