id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298295-epxd03pt	Eckermann, M.	3d Virtual Patho-Histology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography	2020-06-23		.txt	text/plain	6497	369	51	We present a new approach of three-dimensional (3d) virtual histology and patho-histology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal-architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we can propose multi-scale phase contrast x-ray tomography as a novel tool to unravel the patho-physiology of Covid-19, extending conventional histology by a third dimension and allowing for a full quantification of tissue remodeling.By combining parallel and cone beam geometry, autopsy samples with a cross section of 4mm are scanned and reconstructed at a resolution and image quality which allows for the segmentation of individual cells. The 3d virtual pathohistology approach for Covid-19 presented here was realized by implementing a novel multi-scale phase contrast x-ray tomography concept, with dedicated xray optics and instrumentation to image the tissue structure on multiple length scales, while at the same time covering large reconstruction volumes.	./cache/cord-298295-epxd03pt.txt	./txt/cord-298295-epxd03pt.txt