id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103735-nil1vv6h	Alfano, Niccolo	Non-invasive surveys of mammalian viruses using environmental DNA	2020-03-29		.txt	text/plain	5829	373	53	Using a curated set of RNA oligonucleotides based on the ViroChip microarray assay [7] as baits in a hybridization capture system, multiple mammalian RNA and DNA viruses were detected from both eDNA and iDNA samples. Highlights Environmental DNA (water and blood-sucking leeches) provided a non-invasive method of screening wildlife for viruses A comprehensive viral RNA oligonucleotide bait set was developed to capture known and unknown mammalian virus diversity Leech blood meal host determination and viruses identified were congruent Viruses determined from water correlated with known and observed species visiting the water sources In brief Alfano, Dayaram, et al. In filtered water and sediment samples collected from the same waterhole, only one virus per sample was generally identified and in one location (WM20 and SM20) contigs from different viral families were isolated based on sample type. We provide evidence that environmental and invertebrate-derived DNA samples including waterhole water, sediment and wild haematophagous terrestrial leeches can be used to survey known and unknown viruses.	./cache/cord-103735-nil1vv6h.txt	./txt/cord-103735-nil1vv6h.txt