id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002178-ggtxuulg	Mauk, Michael G.	Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification	2015-10-20		.txt	text/plain	5753	257	39	A portable, miniaturized POC NAAT with performance comparable to conventional PCR (polymerase-chain reaction)-based tests in clinical laboratories can be realized with a disposable, palm-sized, plastic microfluidic chip in which: (1) nucleic acids (NAs) are extracted from relatively large (~mL) volume sample lysates using an embedded porous silica glass fiber or cellulose binding phase ("membrane") to capture sample NAs in a flow-through, filtration mode; (2) NAs captured on the membrane are isothermally (~65 °C) amplified; (3) amplicon production is monitored by real-time fluorescence detection, such as with a smartphone CCD camera serving as a low-cost detector; and (4) paraffin-encapsulated, lyophilized reagents for temperature-activated release are pre-stored in the chip.	./cache/cord-002178-ggtxuulg.txt	./txt/cord-002178-ggtxuulg.txt