id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000799-s70onyix	Wang, Ting	Particulate matter air pollution disrupts endothelial cell barrier via calpain-mediated tight junction protein degradation	2012-08-29		.txt	text/plain	5884	363	46	title: Particulate matter air pollution disrupts endothelial cell barrier via calpain-mediated tight junction protein degradation OBJECTIVES: We explored the role of tight junction proteins as targets for PM-induced loss of lung endothelial cell (EC) barrier integrity and enhanced cardiopulmonary dysfunction. We investigated the role of ROS and calpain in PM-induced pulmonary inflammation by examining protein leakage, white blood cell infiltration (inflammatory leukocytes), and the release of proinflammatory cytokines into BAL fluids ( Figure 5 ). These data represent the first evidence that calpain signaling, via calcium leakage from activated TRPM2 by ROS, plays a critical role in modulating endothelial cell barrier function, resulting in tight junction protein ZO-1 degradation (Additional file 1 Figure S7 ). Particulate matter induces endothelial cell intracellular oxidative stress, which leads to the activation of calpain, one of the major cytoskeletal regulators.	./cache/cord-000799-s70onyix.txt	./txt/cord-000799-s70onyix.txt