id	sid	tid	token	lemma	pos
cord-016179-4i1n9j4x	1	1	key	key	NN
cord-016179-4i1n9j4x	1	2	:	:	:
cord-016179-4i1n9j4x	2	1	cord-016179	cord-016179	NNP
cord-016179-4i1n9j4x	2	2	-	-	HYPH
cord-016179-4i1n9j4x	2	3	4i1n9j4x	4i1n9j4x	CD
cord-016179-4i1n9j4x	2	4	authors	author	NNS
cord-016179-4i1n9j4x	2	5	:	:	:
cord-016179-4i1n9j4x	2	6	Chen	Chen	NNP
cord-016179-4i1n9j4x	2	7	,	,	,
cord-016179-4i1n9j4x	2	8	Yi	Yi	NNP
cord-016179-4i1n9j4x	2	9	-	-	HYPH
cord-016179-4i1n9j4x	2	10	Ning	Ning	NNP
cord-016179-4i1n9j4x	2	11	;	;	:
cord-016179-4i1n9j4x	2	12	Wu	Wu	NNP
cord-016179-4i1n9j4x	2	13	,	,	,
cord-016179-4i1n9j4x	2	14	Ching	Ching	NNP
cord-016179-4i1n9j4x	2	15	Ching	Ching	NNP
cord-016179-4i1n9j4x	2	16	;	;	:
cord-016179-4i1n9j4x	2	17	Lin	Lin	NNP
cord-016179-4i1n9j4x	2	18	,	,	,
cord-016179-4i1n9j4x	3	1	Tsang	Tsang	NNP
cord-016179-4i1n9j4x	3	2	Long	Long	NNP
cord-016179-4i1n9j4x	3	3	title	title	NN
cord-016179-4i1n9j4x	3	4	:	:	:
cord-016179-4i1n9j4x	3	5	Real	real	JJ
cord-016179-4i1n9j4x	3	6	-	-	HYPH
cord-016179-4i1n9j4x	3	7	Time	time	NN
cord-016179-4i1n9j4x	3	8	Reverse	Reverse	NNP
cord-016179-4i1n9j4x	3	9	Transcription	Transcription	NNP
cord-016179-4i1n9j4x	3	10	-	-	HYPH
cord-016179-4i1n9j4x	3	11	Polymerase	Polymerase	NNP
cord-016179-4i1n9j4x	3	12	Chain	Chain	NNP
cord-016179-4i1n9j4x	3	13	Reaction	reaction	NN
cord-016179-4i1n9j4x	3	14	for	for	IN
cord-016179-4i1n9j4x	3	15	Detection	Detection	NNP
cord-016179-4i1n9j4x	3	16	and	and	CC
cord-016179-4i1n9j4x	3	17	Quantitation	Quantitation	NNP
cord-016179-4i1n9j4x	3	18	of	of	IN
cord-016179-4i1n9j4x	3	19	Turkey	Turkey	NNP
cord-016179-4i1n9j4x	3	20	Coronavirus	Coronavirus	NNP
cord-016179-4i1n9j4x	3	21	RNA	RNA	NNP
cord-016179-4i1n9j4x	3	22	in	in	IN
cord-016179-4i1n9j4x	3	23	Feces	fece	NNS
cord-016179-4i1n9j4x	3	24	and	and	CC
cord-016179-4i1n9j4x	3	25	Intestine	intestine	NN
cord-016179-4i1n9j4x	3	26	Tissues	Tissues	NNP
cord-016179-4i1n9j4x	3	27	date	date	NN
cord-016179-4i1n9j4x	3	28	:	:	:
cord-016179-4i1n9j4x	3	29	2015	2015	CD
cord-016179-4i1n9j4x	3	30	-	-	HYPH
cord-016179-4i1n9j4x	3	31	09	09	CD
cord-016179-4i1n9j4x	3	32	-	-	SYM
cord-016179-4i1n9j4x	3	33	10	10	CD
cord-016179-4i1n9j4x	3	34	journal	journal	NN
cord-016179-4i1n9j4x	3	35	:	:	:
cord-016179-4i1n9j4x	4	1	Animal	Animal	NNP
cord-016179-4i1n9j4x	4	2	Coronaviruses	Coronaviruses	NNP
cord-016179-4i1n9j4x	4	3	DOI	DOI	NNP
cord-016179-4i1n9j4x	4	4	:	:	:
cord-016179-4i1n9j4x	4	5	10.1007/978	10.1007/978	CD
cord-016179-4i1n9j4x	4	6	-	-	HYPH
cord-016179-4i1n9j4x	4	7	1	1	CD
cord-016179-4i1n9j4x	4	8	-	-	HYPH
cord-016179-4i1n9j4x	4	9	4939	4939	CD
cord-016179-4i1n9j4x	4	10	-	-	HYPH
cord-016179-4i1n9j4x	4	11	3414	3414	CD
cord-016179-4i1n9j4x	4	12	-	-	HYPH
cord-016179-4i1n9j4x	4	13	0_13	0_13	CD
cord-016179-4i1n9j4x	5	1	sha	sha	NNP
cord-016179-4i1n9j4x	5	2	:	:	:
cord-016179-4i1n9j4x	6	1	7eb5dfd9186a6d77260cc08c45f01c2438ca288c	7eb5dfd9186a6d77260cc08c45f01c2438ca288c	NNP
cord-016179-4i1n9j4x	6	2	doc_id	doc_id	CD
cord-016179-4i1n9j4x	6	3	:	:	:
cord-016179-4i1n9j4x	6	4	16179	16179	CD
cord-016179-4i1n9j4x	6	5	cord_uid	cord_uid	NNS
cord-016179-4i1n9j4x	6	6	:	:	:
cord-016179-4i1n9j4x	7	1	4i1n9j4x	4i1n9j4x	CD
cord-016179-4i1n9j4x	8	1	Turkey	Turkey	NNP
cord-016179-4i1n9j4x	8	2	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	9	1	(	(	-LRB-
cord-016179-4i1n9j4x	9	2	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	9	3	)	)	-RRB-
cord-016179-4i1n9j4x	9	4	infection	infection	NN
cord-016179-4i1n9j4x	9	5	causes	cause	VBZ
cord-016179-4i1n9j4x	9	6	acute	acute	JJ
cord-016179-4i1n9j4x	9	7	atrophic	atrophic	JJ
cord-016179-4i1n9j4x	9	8	enteritis	enteritis	NN
cord-016179-4i1n9j4x	9	9	in	in	IN
cord-016179-4i1n9j4x	9	10	turkey	turkey	NNP
cord-016179-4i1n9j4x	9	11	poults	poult	NNS
cord-016179-4i1n9j4x	9	12	,	,	,
cord-016179-4i1n9j4x	9	13	leading	lead	VBG
cord-016179-4i1n9j4x	9	14	to	to	IN
cord-016179-4i1n9j4x	9	15	significant	significant	JJ
cord-016179-4i1n9j4x	9	16	economic	economic	JJ
cord-016179-4i1n9j4x	9	17	loss	loss	NN
cord-016179-4i1n9j4x	9	18	in	in	IN
cord-016179-4i1n9j4x	9	19	the	the	DT
cord-016179-4i1n9j4x	9	20	turkey	turkey	NN
cord-016179-4i1n9j4x	9	21	industry	industry	NN
cord-016179-4i1n9j4x	9	22	.	.	.
cord-016179-4i1n9j4x	10	1	Rapid	rapid	JJ
cord-016179-4i1n9j4x	10	2	detection	detection	NN
cord-016179-4i1n9j4x	10	3	,	,	,
cord-016179-4i1n9j4x	10	4	differentiation	differentiation	NN
cord-016179-4i1n9j4x	10	5	,	,	,
cord-016179-4i1n9j4x	10	6	and	and	CC
cord-016179-4i1n9j4x	10	7	quantitation	quantitation	NN
cord-016179-4i1n9j4x	10	8	of	of	IN
cord-016179-4i1n9j4x	10	9	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	10	10	are	be	VBP
cord-016179-4i1n9j4x	10	11	critical	critical	JJ
cord-016179-4i1n9j4x	10	12	to	to	IN
cord-016179-4i1n9j4x	10	13	the	the	DT
cord-016179-4i1n9j4x	10	14	diagnosis	diagnosis	NN
cord-016179-4i1n9j4x	10	15	and	and	CC
cord-016179-4i1n9j4x	10	16	control	control	NN
cord-016179-4i1n9j4x	10	17	of	of	IN
cord-016179-4i1n9j4x	10	18	the	the	DT
cord-016179-4i1n9j4x	10	19	disease	disease	NN
cord-016179-4i1n9j4x	10	20	.	.	.
cord-016179-4i1n9j4x	11	1	A	a	DT
cord-016179-4i1n9j4x	11	2	specific	specific	JJ
cord-016179-4i1n9j4x	11	3	one	one	CD
cord-016179-4i1n9j4x	11	4	-	-	HYPH
cord-016179-4i1n9j4x	11	5	step	step	NN
cord-016179-4i1n9j4x	11	6	real	real	JJ
cord-016179-4i1n9j4x	11	7	-	-	HYPH
cord-016179-4i1n9j4x	11	8	time	time	NN
cord-016179-4i1n9j4x	11	9	reverse	reverse	NN
cord-016179-4i1n9j4x	11	10	transcription	transcription	NN
cord-016179-4i1n9j4x	11	11	-	-	HYPH
cord-016179-4i1n9j4x	11	12	polymerase	polymerase	NN
cord-016179-4i1n9j4x	11	13	chain	chain	NN
cord-016179-4i1n9j4x	11	14	reaction	reaction	NN
cord-016179-4i1n9j4x	11	15	(	(	-LRB-
cord-016179-4i1n9j4x	11	16	RT	RT	NNP
cord-016179-4i1n9j4x	11	17	-	-	HYPH
cord-016179-4i1n9j4x	11	18	PCR	PCR	NNP
cord-016179-4i1n9j4x	11	19	)	)	-RRB-
cord-016179-4i1n9j4x	11	20	assay	assay	NN
cord-016179-4i1n9j4x	11	21	using	use	VBG
cord-016179-4i1n9j4x	11	22	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	11	23	-	-	HYPH
cord-016179-4i1n9j4x	11	24	specific	specific	JJ
cord-016179-4i1n9j4x	11	25	primers	primer	NNS
cord-016179-4i1n9j4x	11	26	and	and	CC
cord-016179-4i1n9j4x	11	27	dual	dual	RB
cord-016179-4i1n9j4x	11	28	-	-	HYPH
cord-016179-4i1n9j4x	11	29	labeled	label	VBN
cord-016179-4i1n9j4x	11	30	fluorescent	fluorescent	JJ
cord-016179-4i1n9j4x	11	31	probe	probe	NN
cord-016179-4i1n9j4x	11	32	for	for	IN
cord-016179-4i1n9j4x	11	33	detection	detection	NN
cord-016179-4i1n9j4x	11	34	and	and	CC
cord-016179-4i1n9j4x	11	35	quantitation	quantitation	NN
cord-016179-4i1n9j4x	11	36	of	of	IN
cord-016179-4i1n9j4x	11	37	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	11	38	in	in	IN
cord-016179-4i1n9j4x	11	39	feces	fece	NNS
cord-016179-4i1n9j4x	11	40	and	and	CC
cord-016179-4i1n9j4x	11	41	intestine	intestine	NN
cord-016179-4i1n9j4x	11	42	tissues	tissue	NNS
cord-016179-4i1n9j4x	11	43	is	be	VBZ
cord-016179-4i1n9j4x	11	44	described	describe	VBN
cord-016179-4i1n9j4x	11	45	in	in	IN
cord-016179-4i1n9j4x	11	46	this	this	DT
cord-016179-4i1n9j4x	11	47	chapter	chapter	NN
cord-016179-4i1n9j4x	11	48	.	.	.
cord-016179-4i1n9j4x	12	1	The	the	DT
cord-016179-4i1n9j4x	12	2	fluorogenic	fluorogenic	JJ
cord-016179-4i1n9j4x	12	3	probe	probe	NN
cord-016179-4i1n9j4x	12	4	labeled	label	VBN
cord-016179-4i1n9j4x	12	5	with	with	IN
cord-016179-4i1n9j4x	12	6	a	a	DT
cord-016179-4i1n9j4x	12	7	reporter	reporter	NN
cord-016179-4i1n9j4x	12	8	dye	dye	NN
cord-016179-4i1n9j4x	12	9	(	(	-LRB-
cord-016179-4i1n9j4x	12	10	FAM	FAM	NNP
cord-016179-4i1n9j4x	12	11	,	,	,
cord-016179-4i1n9j4x	12	12	6-carboxytetramethylrhodamine	6-carboxytetramethylrhodamine	CD
cord-016179-4i1n9j4x	12	13	)	)	-RRB-
cord-016179-4i1n9j4x	12	14	and	and	CC
cord-016179-4i1n9j4x	12	15	a	a	DT
cord-016179-4i1n9j4x	12	16	quencher	quencher	NN
cord-016179-4i1n9j4x	12	17	dye	dye	NN
cord-016179-4i1n9j4x	12	18	(	(	-LRB-
cord-016179-4i1n9j4x	12	19	Absolute	Absolute	NNP
cord-016179-4i1n9j4x	12	20	Quencher	Quencher	NNP
cord-016179-4i1n9j4x	12	21	™	™	NN
cord-016179-4i1n9j4x	12	22	)	)	-RRB-
cord-016179-4i1n9j4x	12	23	was	be	VBD
cord-016179-4i1n9j4x	12	24	designed	design	VBN
cord-016179-4i1n9j4x	12	25	to	to	TO
cord-016179-4i1n9j4x	12	26	bind	bind	VB
cord-016179-4i1n9j4x	12	27	to	to	IN
cord-016179-4i1n9j4x	12	28	a	a	DT
cord-016179-4i1n9j4x	12	29	186	186	CD
cord-016179-4i1n9j4x	12	30	base	base	JJ
cord-016179-4i1n9j4x	12	31	-	-	HYPH
cord-016179-4i1n9j4x	12	32	pair	pair	NN
cord-016179-4i1n9j4x	12	33	fragment	fragment	NN
cord-016179-4i1n9j4x	12	34	flanked	flank	VBN
cord-016179-4i1n9j4x	12	35	by	by	IN
cord-016179-4i1n9j4x	12	36	the	the	DT
cord-016179-4i1n9j4x	12	37	two	two	CD
cord-016179-4i1n9j4x	12	38	PCR	PCR	NNP
cord-016179-4i1n9j4x	12	39	primers	primer	NNS
cord-016179-4i1n9j4x	12	40	targeting	target	VBG
cord-016179-4i1n9j4x	12	41	the	the	DT
cord-016179-4i1n9j4x	12	42	3′	3′	CD
cord-016179-4i1n9j4x	12	43	end	end	NN
cord-016179-4i1n9j4x	12	44	of	of	IN
cord-016179-4i1n9j4x	12	45	spike	spike	NN
cord-016179-4i1n9j4x	12	46	gene	gene	NN
cord-016179-4i1n9j4x	12	47	(	(	-LRB-
cord-016179-4i1n9j4x	12	48	S2	S2	NNP
cord-016179-4i1n9j4x	12	49	)	)	-RRB-
cord-016179-4i1n9j4x	12	50	of	of	IN
cord-016179-4i1n9j4x	12	51	TCoV.	TCoV.	NNP
cord-016179-4i1n9j4x	13	1	The	the	DT
cord-016179-4i1n9j4x	13	2	assay	assay	NN
cord-016179-4i1n9j4x	13	3	is	be	VBZ
cord-016179-4i1n9j4x	13	4	highly	highly	RB
cord-016179-4i1n9j4x	13	5	specific	specific	JJ
cord-016179-4i1n9j4x	13	6	and	and	CC
cord-016179-4i1n9j4x	13	7	sensitive	sensitive	JJ
cord-016179-4i1n9j4x	13	8	and	and	CC
cord-016179-4i1n9j4x	13	9	can	can	MD
cord-016179-4i1n9j4x	13	10	quantitate	quantitate	VB
cord-016179-4i1n9j4x	13	11	between	between	IN
cord-016179-4i1n9j4x	13	12	10(2	10(2	CD
cord-016179-4i1n9j4x	13	13	)	)	-RRB-
cord-016179-4i1n9j4x	13	14	and	and	CC
cord-016179-4i1n9j4x	13	15	10(10	10(10	CD
cord-016179-4i1n9j4x	13	16	)	)	-RRB-
cord-016179-4i1n9j4x	13	17	copies	copy	NNS
cord-016179-4i1n9j4x	13	18	/	/	SYM
cord-016179-4i1n9j4x	13	19	mL	ml	NN
cord-016179-4i1n9j4x	13	20	of	of	IN
cord-016179-4i1n9j4x	13	21	viral	viral	JJ
cord-016179-4i1n9j4x	13	22	genome	genome	NN
cord-016179-4i1n9j4x	13	23	.	.	.
cord-016179-4i1n9j4x	14	1	It	-PRON-	PRP
cord-016179-4i1n9j4x	14	2	is	be	VBZ
cord-016179-4i1n9j4x	14	3	useful	useful	JJ
cord-016179-4i1n9j4x	14	4	in	in	IN
cord-016179-4i1n9j4x	14	5	monitoring	monitor	VBG
cord-016179-4i1n9j4x	14	6	the	the	DT
cord-016179-4i1n9j4x	14	7	progression	progression	NN
cord-016179-4i1n9j4x	14	8	of	of	IN
cord-016179-4i1n9j4x	14	9	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	14	10	-	-	HYPH
cord-016179-4i1n9j4x	14	11	induced	induce	VBN
cord-016179-4i1n9j4x	14	12	atrophic	atrophic	JJ
cord-016179-4i1n9j4x	14	13	enteritis	enteritis	NN
cord-016179-4i1n9j4x	14	14	in	in	IN
cord-016179-4i1n9j4x	14	15	the	the	DT
cord-016179-4i1n9j4x	14	16	turkey	turkey	NN
cord-016179-4i1n9j4x	14	17	flocks	flock	NNS
cord-016179-4i1n9j4x	14	18	.	.	.
cord-016179-4i1n9j4x	15	1	Turkey	Turkey	NNP
cord-016179-4i1n9j4x	15	2	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	15	3	(	(	-LRB-
cord-016179-4i1n9j4x	15	4	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	15	5	)	)	-RRB-
cord-016179-4i1n9j4x	15	6	causes	cause	VBZ
cord-016179-4i1n9j4x	15	7	atrophic	atrophic	JJ
cord-016179-4i1n9j4x	15	8	enteritis	enteritis	NN
cord-016179-4i1n9j4x	15	9	in	in	IN
cord-016179-4i1n9j4x	15	10	turkeys	turkey	NNS
cord-016179-4i1n9j4x	15	11	and	and	CC
cord-016179-4i1n9j4x	15	12	outbreaks	outbreak	NNS
cord-016179-4i1n9j4x	15	13	or	or	CC
cord-016179-4i1n9j4x	15	14	cases	case	NNS
cord-016179-4i1n9j4x	15	15	of	of	IN
cord-016179-4i1n9j4x	15	16	turkey	turkey	NNP
cord-016179-4i1n9j4x	15	17	coronaviral	coronaviral	NNP
cord-016179-4i1n9j4x	15	18	enteritis	enteritis	NNP
cord-016179-4i1n9j4x	15	19	occurred	occur	VBD
cord-016179-4i1n9j4x	15	20	and	and	CC
cord-016179-4i1n9j4x	15	21	still	still	RB
cord-016179-4i1n9j4x	15	22	occurs	occur	VBZ
cord-016179-4i1n9j4x	15	23	in	in	IN
cord-016179-4i1n9j4x	15	24	the	the	DT
cord-016179-4i1n9j4x	15	25	USA	USA	NNP
cord-016179-4i1n9j4x	16	1	[	[	-LRB-
cord-016179-4i1n9j4x	16	2	1	1	CD
cord-016179-4i1n9j4x	16	3	]	]	-RRB-
cord-016179-4i1n9j4x	16	4	,	,	,
cord-016179-4i1n9j4x	16	5	Canada	Canada	NNP
cord-016179-4i1n9j4x	16	6	[	[	-LRB-
cord-016179-4i1n9j4x	16	7	2	2	CD
cord-016179-4i1n9j4x	16	8	]	]	-RRB-
cord-016179-4i1n9j4x	16	9	,	,	,
cord-016179-4i1n9j4x	16	10	Brazil	Brazil	NNP
cord-016179-4i1n9j4x	16	11	[	[	-LRB-
cord-016179-4i1n9j4x	16	12	3	3	CD
cord-016179-4i1n9j4x	16	13	]	]	-RRB-
cord-016179-4i1n9j4x	16	14	,	,	,
cord-016179-4i1n9j4x	16	15	and	and	CC
cord-016179-4i1n9j4x	16	16	Europe	Europe	NNP
cord-016179-4i1n9j4x	16	17	[	[	-LRB-
cord-016179-4i1n9j4x	16	18	4	4	CD
cord-016179-4i1n9j4x	16	19	]	]	-RRB-
cord-016179-4i1n9j4x	16	20	.	.	.
cord-016179-4i1n9j4x	17	1	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	17	2	belongs	belong	VBZ
cord-016179-4i1n9j4x	17	3	to	to	TO
cord-016179-4i1n9j4x	17	4	species	species	VB
cord-016179-4i1n9j4x	17	5	Avian	avian	JJ
cord-016179-4i1n9j4x	17	6	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	17	7	of	of	IN
cord-016179-4i1n9j4x	17	8	the	the	DT
cord-016179-4i1n9j4x	17	9	genus	genus	NN
cord-016179-4i1n9j4x	17	10	Gammacoronavirus	Gammacoronavirus	NNP
cord-016179-4i1n9j4x	17	11	in	in	IN
cord-016179-4i1n9j4x	17	12	the	the	DT
cord-016179-4i1n9j4x	17	13	family	family	NN
cord-016179-4i1n9j4x	17	14	Coronaviridae	Coronaviridae	NNP
cord-016179-4i1n9j4x	17	15	.	.	.
cord-016179-4i1n9j4x	18	1	The	the	DT
cord-016179-4i1n9j4x	18	2	genome	genome	NN
cord-016179-4i1n9j4x	18	3	of	of	IN
cord-016179-4i1n9j4x	18	4	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	18	5	is	be	VBZ
cord-016179-4i1n9j4x	18	6	a	a	DT
cord-016179-4i1n9j4x	18	7	linear	linear	JJ
cord-016179-4i1n9j4x	18	8	positive	positive	JJ
cord-016179-4i1n9j4x	18	9	-	-	HYPH
cord-016179-4i1n9j4x	18	10	sense	sense	NN
cord-016179-4i1n9j4x	18	11	single	single	JJ
cord-016179-4i1n9j4x	18	12	-	-	HYPH
cord-016179-4i1n9j4x	18	13	stranded	strand	VBN
cord-016179-4i1n9j4x	18	14	RNA	rna	NN
cord-016179-4i1n9j4x	18	15	encoding	encode	VBG
cord-016179-4i1n9j4x	18	16	three	three	CD
cord-016179-4i1n9j4x	18	17	major	major	JJ
cord-016179-4i1n9j4x	18	18	structural	structural	JJ
cord-016179-4i1n9j4x	18	19	proteins	protein	NNS
cord-016179-4i1n9j4x	18	20	including	include	VBG
cord-016179-4i1n9j4x	18	21	spike	spike	NN
cord-016179-4i1n9j4x	18	22	(	(	-LRB-
cord-016179-4i1n9j4x	18	23	S	S	NNP
cord-016179-4i1n9j4x	18	24	)	)	-RRB-
cord-016179-4i1n9j4x	18	25	,	,	,
cord-016179-4i1n9j4x	18	26	membrane	membrane	NN
cord-016179-4i1n9j4x	18	27	(	(	-LRB-
cord-016179-4i1n9j4x	18	28	M	M	NNP
cord-016179-4i1n9j4x	18	29	)	)	-RRB-
cord-016179-4i1n9j4x	18	30	,	,	,
cord-016179-4i1n9j4x	18	31	and	and	CC
cord-016179-4i1n9j4x	18	32	nucleocapsid	nucleocapsid	NN
cord-016179-4i1n9j4x	18	33	(	(	-LRB-
cord-016179-4i1n9j4x	18	34	N	N	NNP
cord-016179-4i1n9j4x	18	35	)	)	-RRB-
cord-016179-4i1n9j4x	18	36	protein	protein	NN
cord-016179-4i1n9j4x	18	37	.	.	.
cord-016179-4i1n9j4x	19	1	The	the	DT
cord-016179-4i1n9j4x	19	2	amino	amino	NN
cord-016179-4i1n9j4x	19	3	terminal	terminal	NN
cord-016179-4i1n9j4x	19	4	region	region	NN
cord-016179-4i1n9j4x	19	5	of	of	IN
cord-016179-4i1n9j4x	19	6	S	S	NNP
cord-016179-4i1n9j4x	19	7	protein	protein	NN
cord-016179-4i1n9j4x	19	8	(	(	-LRB-
cord-016179-4i1n9j4x	19	9	S1	s1	NN
cord-016179-4i1n9j4x	19	10	)	)	-RRB-
cord-016179-4i1n9j4x	19	11	containing	contain	VBG
cord-016179-4i1n9j4x	19	12	receptor	receptor	NN
cord-016179-4i1n9j4x	19	13	-	-	HYPH
cord-016179-4i1n9j4x	19	14	binding	bind	VBG
cord-016179-4i1n9j4x	19	15	domain	domain	NN
cord-016179-4i1n9j4x	19	16	and	and	CC
cord-016179-4i1n9j4x	19	17	neutralizing	neutralize	VBG
cord-016179-4i1n9j4x	19	18	epitopes	epitope	NNS
cord-016179-4i1n9j4x	19	19	can	can	MD
cord-016179-4i1n9j4x	19	20	determine	determine	VB
cord-016179-4i1n9j4x	19	21	host	host	NN
cord-016179-4i1n9j4x	19	22	specificity	specificity	NN
cord-016179-4i1n9j4x	19	23	and	and	CC
cord-016179-4i1n9j4x	19	24	induce	induce	VB
cord-016179-4i1n9j4x	19	25	the	the	DT
cord-016179-4i1n9j4x	19	26	production	production	NN
cord-016179-4i1n9j4x	19	27	of	of	IN
cord-016179-4i1n9j4x	19	28	neutralizing	neutralize	VBG
cord-016179-4i1n9j4x	19	29	antibodies	antibody	NNS
cord-016179-4i1n9j4x	19	30	[	[	-LRB-
cord-016179-4i1n9j4x	19	31	5	5	CD
cord-016179-4i1n9j4x	19	32	]	]	-RRB-
cord-016179-4i1n9j4x	19	33	.	.	.
cord-016179-4i1n9j4x	20	1	The	the	DT
cord-016179-4i1n9j4x	20	2	carboxyl	carboxyl	NN
cord-016179-4i1n9j4x	20	3	terminal	terminal	NN
cord-016179-4i1n9j4x	20	4	region	region	NN
cord-016179-4i1n9j4x	20	5	of	of	IN
cord-016179-4i1n9j4x	20	6	S	S	NNP
cord-016179-4i1n9j4x	20	7	protein	protein	NN
cord-016179-4i1n9j4x	20	8	(	(	-LRB-
cord-016179-4i1n9j4x	20	9	S2	s2	NN
cord-016179-4i1n9j4x	20	10	)	)	-RRB-
cord-016179-4i1n9j4x	20	11	consisting	consist	VBG
cord-016179-4i1n9j4x	20	12	of	of	IN
cord-016179-4i1n9j4x	20	13	transmembrane	transmembrane	NN
cord-016179-4i1n9j4x	20	14	domain	domain	NN
cord-016179-4i1n9j4x	20	15	is	be	VBZ
cord-016179-4i1n9j4x	20	16	responsible	responsible	JJ
cord-016179-4i1n9j4x	20	17	for	for	IN
cord-016179-4i1n9j4x	20	18	cell	cell	NN
cord-016179-4i1n9j4x	20	19	fusion	fusion	NN
cord-016179-4i1n9j4x	20	20	and	and	CC
cord-016179-4i1n9j4x	20	21	virus	virus	NN
cord-016179-4i1n9j4x	20	22	assembly	assembly	NN
cord-016179-4i1n9j4x	20	23	[	[	-LRB-
cord-016179-4i1n9j4x	20	24	6	6	CD
cord-016179-4i1n9j4x	20	25	]	]	-RRB-
cord-016179-4i1n9j4x	20	26	.	.	.
cord-016179-4i1n9j4x	21	1	S	s	NN
cord-016179-4i1n9j4x	21	2	gene	gene	NN
cord-016179-4i1n9j4x	21	3	is	be	VBZ
cord-016179-4i1n9j4x	21	4	a	a	DT
cord-016179-4i1n9j4x	21	5	more	more	RBR
cord-016179-4i1n9j4x	21	6	common	common	JJ
cord-016179-4i1n9j4x	21	7	target	target	NN
cord-016179-4i1n9j4x	21	8	used	use	VBN
cord-016179-4i1n9j4x	21	9	for	for	IN
cord-016179-4i1n9j4x	21	10	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	21	11	(	(	-LRB-
cord-016179-4i1n9j4x	21	12	CoV	cov	NN
cord-016179-4i1n9j4x	21	13	)	)	-RRB-
cord-016179-4i1n9j4x	21	14	differentiation	differentiation	NN
cord-016179-4i1n9j4x	21	15	because	because	IN
cord-016179-4i1n9j4x	21	16	S	s	NN
cord-016179-4i1n9j4x	21	17	gene	gene	NN
cord-016179-4i1n9j4x	21	18	is	be	VBZ
cord-016179-4i1n9j4x	21	19	highly	highly	RB
cord-016179-4i1n9j4x	21	20	variable	variable	JJ
cord-016179-4i1n9j4x	21	21	among	among	IN
cord-016179-4i1n9j4x	21	22	different	different	JJ
cord-016179-4i1n9j4x	21	23	CoVs	CoVs	NNP
cord-016179-4i1n9j4x	21	24	while	while	IN
cord-016179-4i1n9j4x	21	25	M	M	NNP
cord-016179-4i1n9j4x	21	26	and	and	CC
cord-016179-4i1n9j4x	21	27	N	n	CD
cord-016179-4i1n9j4x	21	28	genes	gene	NNS
cord-016179-4i1n9j4x	21	29	are	be	VBP
cord-016179-4i1n9j4x	21	30	more	more	RBR
cord-016179-4i1n9j4x	21	31	conserved	conserve	VBN
cord-016179-4i1n9j4x	21	32	.	.	.
cord-016179-4i1n9j4x	22	1	Within	within	IN
cord-016179-4i1n9j4x	22	2	the	the	DT
cord-016179-4i1n9j4x	22	3	S	S	NNP
cord-016179-4i1n9j4x	22	4	gene	gene	NN
cord-016179-4i1n9j4x	22	5	,	,	,
cord-016179-4i1n9j4x	22	6	the	the	DT
cord-016179-4i1n9j4x	22	7	S2	s2	NN
cord-016179-4i1n9j4x	22	8	gene	gene	NN
cord-016179-4i1n9j4x	22	9	is	be	VBZ
cord-016179-4i1n9j4x	22	10	more	more	RBR
cord-016179-4i1n9j4x	22	11	conserved	conserved	JJ
cord-016179-4i1n9j4x	22	12	than	than	IN
cord-016179-4i1n9j4x	22	13	S1	s1	NN
cord-016179-4i1n9j4x	22	14	gene	gene	NN
cord-016179-4i1n9j4x	22	15	between	between	IN
cord-016179-4i1n9j4x	22	16	different	different	JJ
cord-016179-4i1n9j4x	22	17	CoVs	CoVs	NNP
cord-016179-4i1n9j4x	22	18	and	and	CC
cord-016179-4i1n9j4x	22	19	between	between	IN
cord-016179-4i1n9j4x	22	20	different	different	JJ
cord-016179-4i1n9j4x	22	21	isolates	isolate	NNS
cord-016179-4i1n9j4x	22	22	or	or	CC
cord-016179-4i1n9j4x	22	23	strains	strain	NNS
cord-016179-4i1n9j4x	22	24	of	of	IN
cord-016179-4i1n9j4x	22	25	the	the	DT
cord-016179-4i1n9j4x	22	26	same	same	JJ
cord-016179-4i1n9j4x	22	27	CoV	cov	NN
cord-016179-4i1n9j4x	22	28	[	[	-LRB-
cord-016179-4i1n9j4x	22	29	7	7	CD
cord-016179-4i1n9j4x	22	30	]	]	-RRB-
cord-016179-4i1n9j4x	22	31	.	.	.
cord-016179-4i1n9j4x	23	1	Therefore	therefore	RB
cord-016179-4i1n9j4x	23	2	,	,	,
cord-016179-4i1n9j4x	23	3	S2	S2	NNP
cord-016179-4i1n9j4x	23	4	gene	gene	NN
cord-016179-4i1n9j4x	23	5	is	be	VBZ
cord-016179-4i1n9j4x	23	6	chosen	choose	VBN
cord-016179-4i1n9j4x	23	7	as	as	IN
cord-016179-4i1n9j4x	23	8	a	a	DT
cord-016179-4i1n9j4x	23	9	target	target	NN
cord-016179-4i1n9j4x	23	10	to	to	TO
cord-016179-4i1n9j4x	23	11	detect	detect	VB
cord-016179-4i1n9j4x	23	12	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	23	13	and	and	CC
cord-016179-4i1n9j4x	23	14	differentiate	differentiate	VB
cord-016179-4i1n9j4x	23	15	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	23	16	from	from	IN
cord-016179-4i1n9j4x	23	17	other	other	JJ
cord-016179-4i1n9j4x	23	18	CoVs	CoVs	NNP
cord-016179-4i1n9j4x	23	19	.	.	.
cord-016179-4i1n9j4x	24	1	There	there	EX
cord-016179-4i1n9j4x	24	2	is	be	VBZ
cord-016179-4i1n9j4x	24	3	no	no	DT
cord-016179-4i1n9j4x	24	4	cell	cell	NN
cord-016179-4i1n9j4x	24	5	culture	culture	NN
cord-016179-4i1n9j4x	24	6	system	system	NN
cord-016179-4i1n9j4x	24	7	for	for	IN
cord-016179-4i1n9j4x	24	8	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	24	9	;	;	:
cord-016179-4i1n9j4x	24	10	thus	thus	RB
cord-016179-4i1n9j4x	24	11	virus	virus	NN
cord-016179-4i1n9j4x	24	12	isolation	isolation	NN
cord-016179-4i1n9j4x	24	13	is	be	VBZ
cord-016179-4i1n9j4x	24	14	not	not	RB
cord-016179-4i1n9j4x	24	15	feasible	feasible	JJ
cord-016179-4i1n9j4x	24	16	.	.	.
cord-016179-4i1n9j4x	25	1	Because	because	IN
cord-016179-4i1n9j4x	25	2	the	the	DT
cord-016179-4i1n9j4x	25	3	sequence	sequence	NN
cord-016179-4i1n9j4x	25	4	information	information	NN
cord-016179-4i1n9j4x	25	5	of	of	IN
cord-016179-4i1n9j4x	25	6	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	25	7	is	be	VBZ
cord-016179-4i1n9j4x	25	8	available	available	JJ
cord-016179-4i1n9j4x	25	9	,	,	,
cord-016179-4i1n9j4x	25	10	reverse	reverse	JJ
cord-016179-4i1n9j4x	25	11	transcription	transcription	NN
cord-016179-4i1n9j4x	25	12	-	-	HYPH
cord-016179-4i1n9j4x	25	13	polymerase	polymerase	NN
cord-016179-4i1n9j4x	25	14	chain	chain	NN
cord-016179-4i1n9j4x	25	15	reaction	reaction	NN
cord-016179-4i1n9j4x	25	16	(	(	-LRB-
cord-016179-4i1n9j4x	25	17	RT	RT	NNP
cord-016179-4i1n9j4x	25	18	-	-	HYPH
cord-016179-4i1n9j4x	25	19	PCR)-based	pcr)-base	VBN
cord-016179-4i1n9j4x	25	20	methods	method	NNS
cord-016179-4i1n9j4x	25	21	with	with	IN
cord-016179-4i1n9j4x	25	22	high	high	JJ
cord-016179-4i1n9j4x	25	23	specificity	specificity	NN
cord-016179-4i1n9j4x	25	24	and	and	CC
cord-016179-4i1n9j4x	25	25	sensitivity	sensitivity	NN
cord-016179-4i1n9j4x	25	26	have	have	VBP
cord-016179-4i1n9j4x	25	27	been	be	VBN
cord-016179-4i1n9j4x	25	28	developed	develop	VBN
cord-016179-4i1n9j4x	25	29	[	[	-LRB-
cord-016179-4i1n9j4x	25	30	7	7	CD
cord-016179-4i1n9j4x	25	31	,	,	,
cord-016179-4i1n9j4x	25	32	8	8	CD
cord-016179-4i1n9j4x	25	33	]	]	-RRB-
cord-016179-4i1n9j4x	25	34	.	.	.
cord-016179-4i1n9j4x	26	1	Real	real	JJ
cord-016179-4i1n9j4x	26	2	-	-	HYPH
cord-016179-4i1n9j4x	26	3	time	time	NN
cord-016179-4i1n9j4x	26	4	RT	RT	NNP
cord-016179-4i1n9j4x	26	5	-	-	HYPH
cord-016179-4i1n9j4x	26	6	PCR	PCR	NNP
cord-016179-4i1n9j4x	26	7	illustrated	illustrate	VBN
cord-016179-4i1n9j4x	26	8	here	here	RB
cord-016179-4i1n9j4x	26	9	uses	use	VBZ
cord-016179-4i1n9j4x	26	10	a	a	DT
cord-016179-4i1n9j4x	26	11	pair	pair	NN
cord-016179-4i1n9j4x	26	12	of	of	IN
cord-016179-4i1n9j4x	26	13	TCoV	tcov	NN
cord-016179-4i1n9j4x	26	14	-	-	HYPH
cord-016179-4i1n9j4x	26	15	specific	specific	JJ
cord-016179-4i1n9j4x	26	16	primers	primer	NNS
cord-016179-4i1n9j4x	26	17	targeting	target	VBG
cord-016179-4i1n9j4x	26	18	a	a	DT
cord-016179-4i1n9j4x	26	19	186	186	CD
cord-016179-4i1n9j4x	26	20	base	base	JJ
cord-016179-4i1n9j4x	26	21	-	-	HYPH
cord-016179-4i1n9j4x	26	22	pair	pair	NN
cord-016179-4i1n9j4x	26	23	fragment	fragment	NN
cord-016179-4i1n9j4x	26	24	of	of	IN
cord-016179-4i1n9j4x	26	25	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	26	26	S2	S2	NNP
cord-016179-4i1n9j4x	26	27	gene	gene	NN
cord-016179-4i1n9j4x	26	28	and	and	CC
cord-016179-4i1n9j4x	26	29	a	a	DT
cord-016179-4i1n9j4x	26	30	dual	dual	RB
cord-016179-4i1n9j4x	26	31	-	-	HYPH
cord-016179-4i1n9j4x	26	32	labeled	label	VBN
cord-016179-4i1n9j4x	26	33	probe	probe	NN
cord-016179-4i1n9j4x	26	34	with	with	IN
cord-016179-4i1n9j4x	26	35	a	a	DT
cord-016179-4i1n9j4x	26	36	reporter	reporter	NN
cord-016179-4i1n9j4x	26	37	dye	dye	NN
cord-016179-4i1n9j4x	26	38	(	(	-LRB-
cord-016179-4i1n9j4x	26	39	FAM	FAM	NNP
cord-016179-4i1n9j4x	26	40	)	)	-RRB-
cord-016179-4i1n9j4x	26	41	and	and	CC
cord-016179-4i1n9j4x	26	42	a	a	DT
cord-016179-4i1n9j4x	26	43	quencher	quencher	NN
cord-016179-4i1n9j4x	26	44	dye	dye	NN
cord-016179-4i1n9j4x	26	45	(	(	-LRB-
cord-016179-4i1n9j4x	26	46	Absolute	Absolute	NNP
cord-016179-4i1n9j4x	26	47	Quencher	Quencher	NNP
cord-016179-4i1n9j4x	26	48	™	™	NN
cord-016179-4i1n9j4x	26	49	)	)	-RRB-
cord-016179-4i1n9j4x	26	50	combined	combine	VBN
cord-016179-4i1n9j4x	26	51	with	with	IN
cord-016179-4i1n9j4x	26	52	the	the	DT
cord-016179-4i1n9j4x	26	53	5′	5′	CD
cord-016179-4i1n9j4x	26	54	to	to	IN
cord-016179-4i1n9j4x	26	55	3′	3′	CD
cord-016179-4i1n9j4x	26	56	exonuclease	exonuclease	NN
cord-016179-4i1n9j4x	26	57	activity	activity	NN
cord-016179-4i1n9j4x	26	58	of	of	IN
cord-016179-4i1n9j4x	26	59	Taq	taq	NN
cord-016179-4i1n9j4x	26	60	polymerase	polymerase	NN
cord-016179-4i1n9j4x	26	61	to	to	TO
cord-016179-4i1n9j4x	26	62	increase	increase	VB
cord-016179-4i1n9j4x	26	63	the	the	DT
cord-016179-4i1n9j4x	26	64	release	release	NN
cord-016179-4i1n9j4x	26	65	of	of	IN
cord-016179-4i1n9j4x	26	66	reporter	reporter	NN
cord-016179-4i1n9j4x	26	67	dye	dye	NN
cord-016179-4i1n9j4x	26	68	fluorescence	fluorescence	NN
cord-016179-4i1n9j4x	26	69	in	in	IN
cord-016179-4i1n9j4x	26	70	the	the	DT
cord-016179-4i1n9j4x	26	71	course	course	NN
cord-016179-4i1n9j4x	26	72	of	of	IN
cord-016179-4i1n9j4x	26	73	PCR	PCR	NNP
cord-016179-4i1n9j4x	26	74	amplification	amplification	NN
cord-016179-4i1n9j4x	26	75	[	[	-LRB-
cord-016179-4i1n9j4x	26	76	9	9	CD
cord-016179-4i1n9j4x	26	77	]	]	-RRB-
cord-016179-4i1n9j4x	26	78	.	.	.
cord-016179-4i1n9j4x	27	1	Quantitative	quantitative	JJ
cord-016179-4i1n9j4x	27	2	data	datum	NNS
cord-016179-4i1n9j4x	27	3	can	can	MD
cord-016179-4i1n9j4x	27	4	be	be	VB
cord-016179-4i1n9j4x	27	5	accessed	access	VBN
cord-016179-4i1n9j4x	27	6	by	by	IN
cord-016179-4i1n9j4x	27	7	the	the	DT
cord-016179-4i1n9j4x	27	8	standard	standard	JJ
cord-016179-4i1n9j4x	27	9	curve	curve	NN
cord-016179-4i1n9j4x	27	10	established	establish	VBN
cord-016179-4i1n9j4x	27	11	with	with	IN
cord-016179-4i1n9j4x	27	12	serial	serial	JJ
cord-016179-4i1n9j4x	27	13	dilutions	dilution	NNS
cord-016179-4i1n9j4x	27	14	of	of	IN
cord-016179-4i1n9j4x	27	15	standard	standard	JJ
cord-016179-4i1n9j4x	27	16	RNA	RNA	NNP
cord-016179-4i1n9j4x	27	17	.	.	.
cord-016179-4i1n9j4x	28	1	The	the	DT
cord-016179-4i1n9j4x	28	2	procedure	procedure	NN
cord-016179-4i1n9j4x	28	3	does	do	VBZ
cord-016179-4i1n9j4x	28	4	not	not	RB
cord-016179-4i1n9j4x	28	5	need	need	VB
cord-016179-4i1n9j4x	28	6	post	post	JJ
cord-016179-4i1n9j4x	28	7	-	-	JJ
cord-016179-4i1n9j4x	28	8	PCR	pcr	JJ
cord-016179-4i1n9j4x	28	9	electrophoresis	electrophoresis	NN
cord-016179-4i1n9j4x	28	10	,	,	,
cord-016179-4i1n9j4x	28	11	so	so	CC
cord-016179-4i1n9j4x	28	12	the	the	DT
cord-016179-4i1n9j4x	28	13	processing	processing	NN
cord-016179-4i1n9j4x	28	14	time	time	NN
cord-016179-4i1n9j4x	28	15	can	can	MD
cord-016179-4i1n9j4x	28	16	be	be	VB
cord-016179-4i1n9j4x	28	17	significantly	significantly	RB
cord-016179-4i1n9j4x	28	18	reduced	reduce	VBN
cord-016179-4i1n9j4x	28	19	and	and	CC
cord-016179-4i1n9j4x	28	20	the	the	DT
cord-016179-4i1n9j4x	28	21	risks	risk	NNS
cord-016179-4i1n9j4x	28	22	for	for	IN
cord-016179-4i1n9j4x	28	23	carryover	carryover	NN
cord-016179-4i1n9j4x	28	24	and	and	CC
cord-016179-4i1n9j4x	28	25	cross	cross	NN
cord-016179-4i1n9j4x	28	26	-	-	NN
cord-016179-4i1n9j4x	28	27	contamination	contamination	NN
cord-016179-4i1n9j4x	28	28	between	between	IN
cord-016179-4i1n9j4x	28	29	samples	sample	NNS
cord-016179-4i1n9j4x	28	30	can	can	MD
cord-016179-4i1n9j4x	28	31	be	be	VB
cord-016179-4i1n9j4x	28	32	lessened	lessen	VBN
cord-016179-4i1n9j4x	28	33	.	.	.
cord-016179-4i1n9j4x	29	1	In	in	IN
cord-016179-4i1n9j4x	29	2	this	this	DT
cord-016179-4i1n9j4x	29	3	chapter	chapter	NN
cord-016179-4i1n9j4x	29	4	,	,	,
cord-016179-4i1n9j4x	29	5	the	the	DT
cord-016179-4i1n9j4x	29	6	protocol	protocol	NN
cord-016179-4i1n9j4x	29	7	for	for	IN
cord-016179-4i1n9j4x	29	8	one	one	CD
cord-016179-4i1n9j4x	29	9	-	-	HYPH
cord-016179-4i1n9j4x	29	10	step	step	NN
cord-016179-4i1n9j4x	29	11	real	real	JJ
cord-016179-4i1n9j4x	29	12	-	-	HYPH
cord-016179-4i1n9j4x	29	13	time	time	NN
cord-016179-4i1n9j4x	29	14	RT	rt	NN
cord-016179-4i1n9j4x	29	15	-	-	HYPH
cord-016179-4i1n9j4x	29	16	PCR	PCR	NNP
cord-016179-4i1n9j4x	29	17	to	to	TO
cord-016179-4i1n9j4x	29	18	detect	detect	VB
cord-016179-4i1n9j4x	29	19	,	,	,
cord-016179-4i1n9j4x	29	20	differentiate	differentiate	VB
cord-016179-4i1n9j4x	29	21	,	,	,
cord-016179-4i1n9j4x	29	22	and	and	CC
cord-016179-4i1n9j4x	29	23	quantitative	quantitative	JJ
cord-016179-4i1n9j4x	29	24	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	29	25	RNA	RNA	NNP
cord-016179-4i1n9j4x	29	26	in	in	IN
cord-016179-4i1n9j4x	29	27	the	the	DT
cord-016179-4i1n9j4x	29	28	feces	fece	NNS
cord-016179-4i1n9j4x	29	29	and	and	CC
cord-016179-4i1n9j4x	29	30	intestinal	intestinal	JJ
cord-016179-4i1n9j4x	29	31	tissue	tissue	NN
cord-016179-4i1n9j4x	29	32	is	be	VBZ
cord-016179-4i1n9j4x	29	33	presented	present	VBN
cord-016179-4i1n9j4x	29	34	.	.	.
cord-016179-4i1n9j4x	30	1	In	in	IN
cord-016179-4i1n9j4x	30	2	step	step	NN
cord-016179-4i1n9j4x	30	3	1	1	CD
cord-016179-4i1n9j4x	30	4	,	,	,
cord-016179-4i1n9j4x	30	5	feces	fece	NNS
cord-016179-4i1n9j4x	30	6	or	or	CC
cord-016179-4i1n9j4x	30	7	intestine	intestine	NN
cord-016179-4i1n9j4x	30	8	tissues	tissue	NNS
cord-016179-4i1n9j4x	30	9	were	be	VBD
cord-016179-4i1n9j4x	30	10	collected	collect	VBN
cord-016179-4i1n9j4x	30	11	into	into	IN
cord-016179-4i1n9j4x	30	12	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	30	13	RNA	RNA	NNP
cord-016179-4i1n9j4x	30	14	stabilization	stabilization	NN
cord-016179-4i1n9j4x	30	15	reagent	reagent	NN
cord-016179-4i1n9j4x	30	16	.	.	.
cord-016179-4i1n9j4x	31	1	In	in	IN
cord-016179-4i1n9j4x	31	2	step	step	NN
cord-016179-4i1n9j4x	31	3	2	2	CD
cord-016179-4i1n9j4x	31	4	,	,	,
cord-016179-4i1n9j4x	31	5	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	31	6	RNA	RNA	NNP
cord-016179-4i1n9j4x	31	7	was	be	VBD
cord-016179-4i1n9j4x	31	8	extracted	extract	VBN
cord-016179-4i1n9j4x	31	9	from	from	IN
cord-016179-4i1n9j4x	31	10	feces	fece	NNS
cord-016179-4i1n9j4x	31	11	using	use	VBG
cord-016179-4i1n9j4x	31	12	QIAamp	QIAamp	NNP
cord-016179-4i1n9j4x	31	13	viral	viral	JJ
cord-016179-4i1n9j4x	31	14	RNA	RNA	NNP
cord-016179-4i1n9j4x	31	15	mini	mini	JJ
cord-016179-4i1n9j4x	31	16	kit	kit	NN
cord-016179-4i1n9j4x	31	17	or	or	CC
cord-016179-4i1n9j4x	31	18	intestine	intestine	NN
cord-016179-4i1n9j4x	31	19	tissues	tissue	NNS
cord-016179-4i1n9j4x	31	20	using	use	VBG
cord-016179-4i1n9j4x	31	21	RNAeasy	RNAeasy	NNP
cord-016179-4i1n9j4x	31	22	mini	mini	JJ
cord-016179-4i1n9j4x	31	23	kit	kit	NN
cord-016179-4i1n9j4x	31	24	.	.	.
cord-016179-4i1n9j4x	32	1	In	in	IN
cord-016179-4i1n9j4x	32	2	step	step	NN
cord-016179-4i1n9j4x	32	3	3	3	CD
cord-016179-4i1n9j4x	32	4	,	,	,
cord-016179-4i1n9j4x	32	5	the	the	DT
cord-016179-4i1n9j4x	32	6	extracted	extract	VBN
cord-016179-4i1n9j4x	32	7	RNA	RNA	NNP
cord-016179-4i1n9j4x	32	8	was	be	VBD
cord-016179-4i1n9j4x	32	9	subjected	subject	VBN
cord-016179-4i1n9j4x	32	10	to	to	IN
cord-016179-4i1n9j4x	32	11	one	one	CD
cord-016179-4i1n9j4x	32	12	-	-	HYPH
cord-016179-4i1n9j4x	32	13	step	step	NN
cord-016179-4i1n9j4x	32	14	real	real	JJ
cord-016179-4i1n9j4x	32	15	-	-	HYPH
cord-016179-4i1n9j4x	32	16	time	time	NN
cord-016179-4i1n9j4x	32	17	RT	RT	NNP
cord-016179-4i1n9j4x	32	18	-	-	HYPH
cord-016179-4i1n9j4x	32	19	PCR	PCR	NNP
cord-016179-4i1n9j4x	32	20	for	for	IN
cord-016179-4i1n9j4x	32	21	detection	detection	NN
cord-016179-4i1n9j4x	32	22	and	and	CC
cord-016179-4i1n9j4x	32	23	quantitation	quantitation	NN
cord-016179-4i1n9j4x	32	24	of	of	IN
cord-016179-4i1n9j4x	32	25	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	32	26	in	in	IN
cord-016179-4i1n9j4x	32	27	feces	fece	NNS
cord-016179-4i1n9j4x	32	28	or	or	CC
cord-016179-4i1n9j4x	32	29	intestine	intestine	NN
cord-016179-4i1n9j4x	32	30	tissues	tissue	NNS
cord-016179-4i1n9j4x	32	31	.	.	.
cord-016179-4i1n9j4x	33	1	In	in	IN
cord-016179-4i1n9j4x	33	2	step	step	NN
cord-016179-4i1n9j4x	33	3	4	4	CD
cord-016179-4i1n9j4x	33	4	,	,	,
cord-016179-4i1n9j4x	33	5	a	a	DT
cord-016179-4i1n9j4x	33	6	standard	standard	JJ
cord-016179-4i1n9j4x	33	7	curve	curve	NN
cord-016179-4i1n9j4x	33	8	was	be	VBD
cord-016179-4i1n9j4x	33	9	established	establish	VBN
cord-016179-4i1n9j4x	33	10	by	by	IN
cord-016179-4i1n9j4x	33	11	serially	serially	RB
cord-016179-4i1n9j4x	33	12	diluted	dilute	VBN
cord-016179-4i1n9j4x	33	13	in	in	IN
cord-016179-4i1n9j4x	33	14	vitro	vitro	FW
cord-016179-4i1n9j4x	33	15	-	-	HYPH
cord-016179-4i1n9j4x	33	16	transcribed	transcribe	VBN
cord-016179-4i1n9j4x	33	17	RNA	RNA	NNP
cord-016179-4i1n9j4x	33	18	for	for	IN
cord-016179-4i1n9j4x	33	19	absolute	absolute	JJ
cord-016179-4i1n9j4x	33	20	quantitation	quantitation	NN
cord-016179-4i1n9j4x	33	21	of	of	IN
cord-016179-4i1n9j4x	33	22	TCoV.	TCoV.	NNP
cord-016179-4i1n9j4x	33	23	(	(	-LRB-
cord-016179-4i1n9j4x	33	24	c	c	NN
cord-016179-4i1n9j4x	33	25	)	)	-RRB-
cord-016179-4i1n9j4x	33	26	Add	add	VB
cord-016179-4i1n9j4x	33	27	30	30	CD
cord-016179-4i1n9j4x	33	28	mL	ml	NN
cord-016179-4i1n9j4x	33	29	of	of	IN
cord-016179-4i1n9j4x	33	30	96	96	CD
cord-016179-4i1n9j4x	33	31	-	-	SYM
cord-016179-4i1n9j4x	33	32	100	100	CD
cord-016179-4i1n9j4x	33	33	%	%	NN
cord-016179-4i1n9j4x	33	34	ethanol	ethanol	NN
cord-016179-4i1n9j4x	33	35	into	into	IN
cord-016179-4i1n9j4x	33	36	13	13	CD
cord-016179-4i1n9j4x	33	37	mL	mL	NNS
cord-016179-4i1n9j4x	33	38	of	of	IN
cord-016179-4i1n9j4x	33	39	concentrated	concentrated	JJ
cord-016179-4i1n9j4x	33	40	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	33	41	AW2	AW2	NNP
cord-016179-4i1n9j4x	33	42	.	.	.
cord-016179-4i1n9j4x	34	1	Real	real	JJ
cord-016179-4i1n9j4x	34	2	-	-	HYPH
cord-016179-4i1n9j4x	34	3	Time	time	NN
cord-016179-4i1n9j4x	34	4	RT	RT	NNP
cord-016179-4i1n9j4x	34	5	-	-	HYPH
cord-016179-4i1n9j4x	34	6	PCR	PCR	NNP
cord-016179-4i1n9j4x	34	7	for	for	IN
cord-016179-4i1n9j4x	34	8	Turkey	Turkey	NNP
cord-016179-4i1n9j4x	34	9	Coronavirus	Coronavirus	NNP
cord-016179-4i1n9j4x	34	10	RNA	RNA	NNP
cord-016179-4i1n9j4x	34	11	4	4	CD
cord-016179-4i1n9j4x	34	12	.	.	.
cord-016179-4i1n9j4x	35	1	RNeasy	RNeasy	NNP
cord-016179-4i1n9j4x	35	2	mini	mini	JJ
cord-016179-4i1n9j4x	35	3	kit	kit	NN
cord-016179-4i1n9j4x	35	4	(	(	-LRB-
cord-016179-4i1n9j4x	35	5	Qiagen	Qiagen	NNP
cord-016179-4i1n9j4x	35	6	,	,	,
cord-016179-4i1n9j4x	35	7	Valencia	Valencia	NNP
cord-016179-4i1n9j4x	35	8	,	,	,
cord-016179-4i1n9j4x	35	9	CA	CA	NNP
cord-016179-4i1n9j4x	35	10	,	,	,
cord-016179-4i1n9j4x	35	11	USA	USA	NNP
cord-016179-4i1n9j4x	35	12	)	)	-RRB-
cord-016179-4i1n9j4x	35	13	:	:	:
cord-016179-4i1n9j4x	36	1	The	the	DT
cord-016179-4i1n9j4x	36	2	kit	kit	NN
cord-016179-4i1n9j4x	36	3	contains	contain	VBZ
cord-016179-4i1n9j4x	36	4	Buffer	buffer	NN
cord-016179-4i1n9j4x	36	5	RLT	RLT	NNP
cord-016179-4i1n9j4x	36	6	(	(	-LRB-
cord-016179-4i1n9j4x	36	7	lysis	lysis	NN
cord-016179-4i1n9j4x	36	8	)	)	-RRB-
cord-016179-4i1n9j4x	36	9	,	,	,
cord-016179-4i1n9j4x	36	10	RW1	RW1	NNP
cord-016179-4i1n9j4x	36	11	(	(	-LRB-
cord-016179-4i1n9j4x	36	12	wash	wash	NNP
cord-016179-4i1n9j4x	36	13	)	)	-RRB-
cord-016179-4i1n9j4x	36	14	,	,	,
cord-016179-4i1n9j4x	36	15	and	and	CC
cord-016179-4i1n9j4x	36	16	RPE	RPE	NNP
cord-016179-4i1n9j4x	36	17	(	(	-LRB-
cord-016179-4i1n9j4x	36	18	elute	elute	VB
cord-016179-4i1n9j4x	36	19	)	)	-RRB-
cord-016179-4i1n9j4x	36	20	.	.	.
cord-016179-4i1n9j4x	37	1	Buffer	buffer	NN
cord-016179-4i1n9j4x	37	2	RLT	RLT	NNP
cord-016179-4i1n9j4x	37	3	and	and	CC
cord-016179-4i1n9j4x	37	4	AW1	aw1	NN
cord-016179-4i1n9j4x	37	5	contain	contain	VBP
cord-016179-4i1n9j4x	37	6	guanidine	guanidine	NN
cord-016179-4i1n9j4x	37	7	thiocyanate	thiocyanate	NN
cord-016179-4i1n9j4x	37	8	,	,	,
cord-016179-4i1n9j4x	37	9	which	which	WDT
cord-016179-4i1n9j4x	37	10	is	be	VBZ
cord-016179-4i1n9j4x	37	11	harmful	harmful	JJ
cord-016179-4i1n9j4x	37	12	.	.	.
cord-016179-4i1n9j4x	38	1	RNA	RNA	NNP
cord-016179-4i1n9j4x	38	2	from	from	IN
cord-016179-4i1n9j4x	38	3	intestine	intestine	NN
cord-016179-4i1n9j4x	38	4	tissues	tissue	NNS
cord-016179-4i1n9j4x	38	5	can	can	MD
cord-016179-4i1n9j4x	38	6	bind	bind	VB
cord-016179-4i1n9j4x	38	7	to	to	IN
cord-016179-4i1n9j4x	38	8	silica	silica	NN
cord-016179-4i1n9j4x	38	9	membrane	membrane	NN
cord-016179-4i1n9j4x	38	10	of	of	IN
cord-016179-4i1n9j4x	38	11	RNeasy	RNeasy	NNP
cord-016179-4i1n9j4x	38	12	spin	spin	NN
cord-016179-4i1n9j4x	38	13	columns	column	NNS
cord-016179-4i1n9j4x	38	14	with	with	IN
cord-016179-4i1n9j4x	38	15	a	a	DT
cord-016179-4i1n9j4x	38	16	binding	binding	JJ
cord-016179-4i1n9j4x	38	17	capacity	capacity	NN
cord-016179-4i1n9j4x	38	18	of	of	IN
cord-016179-4i1n9j4x	38	19	100	100	CD
cord-016179-4i1n9j4x	38	20	μg	μg	NNS
cord-016179-4i1n9j4x	38	21	.	.	.
cord-016179-4i1n9j4x	39	1	(	(	-LRB-
cord-016179-4i1n9j4x	39	2	a	a	LS
cord-016179-4i1n9j4x	39	3	)	)	-RRB-
cord-016179-4i1n9j4x	39	4	Add	add	VB
cord-016179-4i1n9j4x	39	5	10	10	CD
cord-016179-4i1n9j4x	40	1	μL	μL	NFP
cord-016179-4i1n9j4x	40	2	β	β	NN
cord-016179-4i1n9j4x	40	3	-	-	HYPH
cord-016179-4i1n9j4x	40	4	mercaptoethanol	mercaptoethanol	NNP
cord-016179-4i1n9j4x	40	5	(	(	-LRB-
cord-016179-4i1n9j4x	40	6	β	β	NN
cord-016179-4i1n9j4x	40	7	-	-	HYPH
cord-016179-4i1n9j4x	40	8	ME	ME	NNP
cord-016179-4i1n9j4x	40	9	)	)	-RRB-
cord-016179-4i1n9j4x	40	10	per	per	IN
cord-016179-4i1n9j4x	40	11	1	1	CD
cord-016179-4i1n9j4x	40	12	mL	ml	NN
cord-016179-4i1n9j4x	40	13	Buffer	buffer	NN
cord-016179-4i1n9j4x	40	14	RLT	RLT	NNP
cord-016179-4i1n9j4x	40	15	in	in	IN
cord-016179-4i1n9j4x	40	16	a	a	DT
cord-016179-4i1n9j4x	40	17	fume	fume	NN
cord-016179-4i1n9j4x	40	18	hood	hood	NN
cord-016179-4i1n9j4x	40	19	.	.	.
cord-016179-4i1n9j4x	41	1	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	41	2	BLT	BLT	NNP
cord-016179-4i1n9j4x	41	3	with	with	IN
cord-016179-4i1n9j4x	41	4	β	β	NN
cord-016179-4i1n9j4x	41	5	-	-	:
cord-016179-4i1n9j4x	41	6	ME	ME	NNP
cord-016179-4i1n9j4x	41	7	can	can	MD
cord-016179-4i1n9j4x	41	8	be	be	VB
cord-016179-4i1n9j4x	41	9	stored	store	VBN
cord-016179-4i1n9j4x	41	10	at	at	IN
cord-016179-4i1n9j4x	41	11	room	room	NN
cord-016179-4i1n9j4x	41	12	temperature	temperature	NN
cord-016179-4i1n9j4x	41	13	for	for	IN
cord-016179-4i1n9j4x	41	14	up	up	IN
cord-016179-4i1n9j4x	41	15	to	to	TO
cord-016179-4i1n9j4x	41	16	1	1	CD
cord-016179-4i1n9j4x	41	17	month	month	NN
cord-016179-4i1n9j4x	41	18	.	.	.
cord-016179-4i1n9j4x	42	1	(	(	-LRB-
cord-016179-4i1n9j4x	42	2	b	b	LS
cord-016179-4i1n9j4x	42	3	)	)	-RRB-
cord-016179-4i1n9j4x	42	4	Add	add	VB
cord-016179-4i1n9j4x	42	5	44	44	CD
cord-016179-4i1n9j4x	42	6	mL	ml	NN
cord-016179-4i1n9j4x	42	7	of	of	IN
cord-016179-4i1n9j4x	42	8	96	96	CD
cord-016179-4i1n9j4x	42	9	-	-	SYM
cord-016179-4i1n9j4x	42	10	100	100	CD
cord-016179-4i1n9j4x	42	11	%	%	NN
cord-016179-4i1n9j4x	42	12	ethanol	ethanol	NN
cord-016179-4i1n9j4x	42	13	to	to	IN
cord-016179-4i1n9j4x	42	14	11	11	CD
cord-016179-4i1n9j4x	42	15	mL	mL	NNS
cord-016179-4i1n9j4x	42	16	of	of	IN
cord-016179-4i1n9j4x	42	17	concentrated	concentrated	JJ
cord-016179-4i1n9j4x	42	18	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	42	19	RPE	RPE	NNP
cord-016179-4i1n9j4x	42	20	.	.	.
cord-016179-4i1n9j4x	43	1	1	1	LS
cord-016179-4i1n9j4x	43	2	.	.	.
cord-016179-4i1n9j4x	44	1	Use	use	VB
cord-016179-4i1n9j4x	44	2	sterile	sterile	JJ
cord-016179-4i1n9j4x	44	3	forceps	forceps	NNS
cord-016179-4i1n9j4x	44	4	and	and	CC
cord-016179-4i1n9j4x	44	5	scissors	scissor	NNS
cord-016179-4i1n9j4x	44	6	to	to	TO
cord-016179-4i1n9j4x	44	7	cut	cut	VB
cord-016179-4i1n9j4x	44	8	0.5	0.5	CD
cord-016179-4i1n9j4x	44	9	cm	cm	NN
cord-016179-4i1n9j4x	44	10	long	long	JJ
cord-016179-4i1n9j4x	44	11	segment	segment	NN
cord-016179-4i1n9j4x	44	12	of	of	IN
cord-016179-4i1n9j4x	44	13	duodenum	duodenum	NN
cord-016179-4i1n9j4x	44	14	,	,	,
cord-016179-4i1n9j4x	44	15	jejunum	jejunum	NNS
cord-016179-4i1n9j4x	44	16	,	,	,
cord-016179-4i1n9j4x	44	17	ileum	ileum	NN
cord-016179-4i1n9j4x	44	18	,	,	,
cord-016179-4i1n9j4x	44	19	or	or	CC
cord-016179-4i1n9j4x	44	20	cecum	cecum	NN
cord-016179-4i1n9j4x	44	21	.	.	.
cord-016179-4i1n9j4x	45	1	8	8	CD
cord-016179-4i1n9j4x	45	2	.	.	.
cord-016179-4i1n9j4x	46	1	Add	add	VB
cord-016179-4i1n9j4x	46	2	500	500	CD
cord-016179-4i1n9j4x	46	3	μL	μL	NNS
cord-016179-4i1n9j4x	46	4	of	of	IN
cord-016179-4i1n9j4x	46	5	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	46	6	AW1	aw1	NN
cord-016179-4i1n9j4x	46	7	and	and	CC
cord-016179-4i1n9j4x	46	8	centrifuge	centrifuge	NN
cord-016179-4i1n9j4x	46	9	at	at	IN
cord-016179-4i1n9j4x	46	10	8000	8000	CD
cord-016179-4i1n9j4x	46	11	rpm	rpm	NN
cord-016179-4i1n9j4x	46	12	for	for	IN
cord-016179-4i1n9j4x	46	13	1	1	CD
cord-016179-4i1n9j4x	46	14	min	min	NN
cord-016179-4i1n9j4x	46	15	.	.	.
cord-016179-4i1n9j4x	47	1	Place	place	VB
cord-016179-4i1n9j4x	47	2	the	the	DT
cord-016179-4i1n9j4x	47	3	QIAamp	QIAamp	NNP
cord-016179-4i1n9j4x	47	4	spin	spin	NN
cord-016179-4i1n9j4x	47	5	column	column	NN
cord-016179-4i1n9j4x	47	6	into	into	IN
cord-016179-4i1n9j4x	47	7	a	a	DT
cord-016179-4i1n9j4x	47	8	clean	clean	JJ
cord-016179-4i1n9j4x	47	9	2	2	CD
cord-016179-4i1n9j4x	47	10	mL	ml	NN
cord-016179-4i1n9j4x	47	11	collection	collection	NN
cord-016179-4i1n9j4x	47	12	tube	tube	NN
cord-016179-4i1n9j4x	47	13	and	and	CC
cord-016179-4i1n9j4x	47	14	discard	discard	VB
cord-016179-4i1n9j4x	47	15	the	the	DT
cord-016179-4i1n9j4x	47	16	tube	tube	NN
cord-016179-4i1n9j4x	47	17	containing	contain	VBG
cord-016179-4i1n9j4x	47	18	the	the	DT
cord-016179-4i1n9j4x	47	19	filtrate	filtrate	NN
cord-016179-4i1n9j4x	47	20	.	.	.
cord-016179-4i1n9j4x	48	1	9	9	LS
cord-016179-4i1n9j4x	48	2	.	.	.
cord-016179-4i1n9j4x	49	1	Add	add	VB
cord-016179-4i1n9j4x	49	2	500	500	CD
cord-016179-4i1n9j4x	49	3	μL	μL	NNS
cord-016179-4i1n9j4x	49	4	of	of	IN
cord-016179-4i1n9j4x	49	5	Buffer	buffer	NN
cord-016179-4i1n9j4x	49	6	AW2	AW2	NNP
cord-016179-4i1n9j4x	49	7	and	and	CC
cord-016179-4i1n9j4x	49	8	centrifuge	centrifuge	NN
cord-016179-4i1n9j4x	49	9	at	at	IN
cord-016179-4i1n9j4x	49	10	14,000	14,000	CD
cord-016179-4i1n9j4x	49	11	rpm	rpm	NN
cord-016179-4i1n9j4x	49	12	(	(	-LRB-
cord-016179-4i1n9j4x	49	13	18407	18407	CD
cord-016179-4i1n9j4x	49	14	×	×	NN
cord-016179-4i1n9j4x	49	15	g	g	NN
cord-016179-4i1n9j4x	49	16	)	)	-RRB-
cord-016179-4i1n9j4x	49	17	for	for	IN
cord-016179-4i1n9j4x	49	18	3	3	CD
cord-016179-4i1n9j4x	49	19	min	min	NN
cord-016179-4i1n9j4x	49	20	.	.	.
cord-016179-4i1n9j4x	50	1	Change	change	VB
cord-016179-4i1n9j4x	50	2	the	the	DT
cord-016179-4i1n9j4x	50	3	direction	direction	NN
cord-016179-4i1n9j4x	50	4	of	of	IN
cord-016179-4i1n9j4x	50	5	tubes	tube	NNS
cord-016179-4i1n9j4x	50	6	and	and	CC
cord-016179-4i1n9j4x	50	7	centrifuge	centrifuge	NN
cord-016179-4i1n9j4x	50	8	at	at	IN
cord-016179-4i1n9j4x	50	9	14,000	14,000	CD
cord-016179-4i1n9j4x	50	10	rpm	rpm	NN
cord-016179-4i1n9j4x	50	11	for	for	IN
cord-016179-4i1n9j4x	50	12	another	another	DT
cord-016179-4i1n9j4x	50	13	1	1	CD
cord-016179-4i1n9j4x	50	14	min	min	NN
cord-016179-4i1n9j4x	50	15	.	.	.
cord-016179-4i1n9j4x	51	1	10	10	CD
cord-016179-4i1n9j4x	51	2	.	.	.
cord-016179-4i1n9j4x	52	1	Place	place	VB
cord-016179-4i1n9j4x	52	2	the	the	DT
cord-016179-4i1n9j4x	52	3	QIAamp	QIAamp	NNP
cord-016179-4i1n9j4x	52	4	spin	spin	NN
cord-016179-4i1n9j4x	52	5	column	column	NN
cord-016179-4i1n9j4x	52	6	in	in	IN
cord-016179-4i1n9j4x	52	7	a	a	DT
cord-016179-4i1n9j4x	52	8	clean	clean	JJ
cord-016179-4i1n9j4x	52	9	1.7	1.7	CD
cord-016179-4i1n9j4x	52	10	mL	ml	NN
cord-016179-4i1n9j4x	52	11	microcentrifuge	microcentrifuge	NN
cord-016179-4i1n9j4x	52	12	tube	tube	NN
cord-016179-4i1n9j4x	52	13	and	and	CC
cord-016179-4i1n9j4x	52	14	add	add	VB
cord-016179-4i1n9j4x	52	15	60	60	CD
cord-016179-4i1n9j4x	52	16	μL	μL	NNS
cord-016179-4i1n9j4x	52	17	of	of	IN
cord-016179-4i1n9j4x	52	18	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	52	19	AVE	AVE	NNP
cord-016179-4i1n9j4x	52	20	.	.	.
cord-016179-4i1n9j4x	53	1	Incubate	incubate	VB
cord-016179-4i1n9j4x	53	2	at	at	IN
cord-016179-4i1n9j4x	53	3	room	room	NN
cord-016179-4i1n9j4x	53	4	temperature	temperature	NN
cord-016179-4i1n9j4x	53	5	for	for	IN
cord-016179-4i1n9j4x	53	6	1	1	CD
cord-016179-4i1n9j4x	53	7	min	min	NN
cord-016179-4i1n9j4x	53	8	and	and	CC
cord-016179-4i1n9j4x	53	9	centrifuge	centrifuge	NN
cord-016179-4i1n9j4x	53	10	at	at	IN
cord-016179-4i1n9j4x	53	11	8000	8000	CD
cord-016179-4i1n9j4x	53	12	rpm	rpm	NN
cord-016179-4i1n9j4x	53	13	for	for	IN
cord-016179-4i1n9j4x	53	14	1	1	CD
cord-016179-4i1n9j4x	53	15	min	min	NN
cord-016179-4i1n9j4x	53	16	.	.	.
cord-016179-4i1n9j4x	54	1	11	11	CD
cord-016179-4i1n9j4x	54	2	.	.	.
cord-016179-4i1n9j4x	55	1	Take	take	VB
cord-016179-4i1n9j4x	55	2	4	4	CD
cord-016179-4i1n9j4x	56	1	μL	μL	.
cord-016179-4i1n9j4x	57	1	RNA	RNA	NNP
cord-016179-4i1n9j4x	57	2	into	into	IN
cord-016179-4i1n9j4x	57	3	96	96	CD
cord-016179-4i1n9j4x	57	4	μL	μL	NFP
cord-016179-4i1n9j4x	57	5	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	57	6	AVE	AVE	NNP
cord-016179-4i1n9j4x	57	7	and	and	CC
cord-016179-4i1n9j4x	57	8	measure	measure	VB
cord-016179-4i1n9j4x	57	9	the	the	DT
cord-016179-4i1n9j4x	57	10	amount	amount	NN
cord-016179-4i1n9j4x	57	11	of	of	IN
cord-016179-4i1n9j4x	57	12	RNA	RNA	NNP
cord-016179-4i1n9j4x	57	13	by	by	IN
cord-016179-4i1n9j4x	57	14	GeneQuant	GeneQuant	NNP
cord-016179-4i1n9j4x	57	15	following	follow	VBG
cord-016179-4i1n9j4x	57	16	the	the	DT
cord-016179-4i1n9j4x	57	17	manufacturer	manufacturer	NN
cord-016179-4i1n9j4x	57	18	's	's	POS
cord-016179-4i1n9j4x	57	19	instruction	instruction	NN
cord-016179-4i1n9j4x	57	20	.	.	.
cord-016179-4i1n9j4x	58	1	12	12	CD
cord-016179-4i1n9j4x	58	2	.	.	.
cord-016179-4i1n9j4x	59	1	Aliquot	Aliquot	NNP
cord-016179-4i1n9j4x	59	2	the	the	DT
cord-016179-4i1n9j4x	59	3	RNA	RNA	NNP
cord-016179-4i1n9j4x	59	4	into	into	IN
cord-016179-4i1n9j4x	59	5	5	5	CD
cord-016179-4i1n9j4x	59	6	μL	μl	NN
cord-016179-4i1n9j4x	60	1	each	each	DT
cord-016179-4i1n9j4x	60	2	tube	tube	NN
cord-016179-4i1n9j4x	60	3	and	and	CC
cord-016179-4i1n9j4x	60	4	store	store	NN
cord-016179-4i1n9j4x	60	5	in	in	IN
cord-016179-4i1n9j4x	60	6	−80	−80	NNP
cord-016179-4i1n9j4x	60	7	°	°	NN
cord-016179-4i1n9j4x	60	8	C	c	NN
cord-016179-4i1n9j4x	60	9	freezer	freezer	NN
cord-016179-4i1n9j4x	60	10	for	for	IN
cord-016179-4i1n9j4x	60	11	use	use	NN
cord-016179-4i1n9j4x	60	12	in	in	IN
cord-016179-4i1n9j4x	60	13	real	real	JJ
cord-016179-4i1n9j4x	60	14	-	-	HYPH
cord-016179-4i1n9j4x	60	15	time	time	NN
cord-016179-4i1n9j4x	60	16	RT	RT	NNP
cord-016179-4i1n9j4x	60	17	-	-	HYPH
cord-016179-4i1n9j4x	60	18	PCR	PCR	NNP
cord-016179-4i1n9j4x	60	19	.	.	.
cord-016179-4i1n9j4x	61	1	1	1	LS
cord-016179-4i1n9j4x	61	2	.	.	.
cord-016179-4i1n9j4x	62	1	Take	take	VB
cord-016179-4i1n9j4x	62	2	intestine	intestine	NN
cord-016179-4i1n9j4x	62	3	segment	segment	NN
cord-016179-4i1n9j4x	62	4	out	out	IN
cord-016179-4i1n9j4x	62	5	of	of	IN
cord-016179-4i1n9j4x	62	6	1.7	1.7	CD
cord-016179-4i1n9j4x	62	7	mL	ml	NN
cord-016179-4i1n9j4x	62	8	microcentrifuge	microcentrifuge	NN
cord-016179-4i1n9j4x	62	9	tubes	tube	NNS
cord-016179-4i1n9j4x	62	10	containing	contain	VBG
cord-016179-4i1n9j4x	62	11	1	1	CD
cord-016179-4i1n9j4x	62	12	mL	ml	NN
cord-016179-4i1n9j4x	62	13	of	of	IN
cord-016179-4i1n9j4x	62	14	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	62	15	RNA	RNA	NNP
cord-016179-4i1n9j4x	62	16	stabilization	stabilization	NN
cord-016179-4i1n9j4x	62	17	reagent	reagent	NN
cord-016179-4i1n9j4x	62	18	and	and	CC
cord-016179-4i1n9j4x	62	19	put	put	VBD
cord-016179-4i1n9j4x	62	20	the	the	DT
cord-016179-4i1n9j4x	62	21	tissue	tissue	NN
cord-016179-4i1n9j4x	62	22	specimen	specimen	NN
cord-016179-4i1n9j4x	62	23	on	on	IN
cord-016179-4i1n9j4x	62	24	top	top	NN
cord-016179-4i1n9j4x	62	25	of	of	IN
cord-016179-4i1n9j4x	62	26	the	the	DT
cord-016179-4i1n9j4x	62	27	plastic	plastic	JJ
cord-016179-4i1n9j4x	62	28	paper	paper	NN
cord-016179-4i1n9j4x	62	29	treated	treat	VBN
cord-016179-4i1n9j4x	62	30	with	with	IN
cord-016179-4i1n9j4x	62	31	RNase	RNase	NNP
cord-016179-4i1n9j4x	62	32	Away	away	RB
cord-016179-4i1n9j4x	62	33	and	and	CC
cord-016179-4i1n9j4x	62	34	DEPC	DEPC	NNP
cord-016179-4i1n9j4x	62	35	H	H	NNP
cord-016179-4i1n9j4x	62	36	2	2	CD
cord-016179-4i1n9j4x	62	37	O.	O.	NNP
cord-016179-4i1n9j4x	62	38	14	14	CD
cord-016179-4i1n9j4x	62	39	.	.	.
cord-016179-4i1n9j4x	63	1	Aliquot	Aliquot	NNP
cord-016179-4i1n9j4x	63	2	the	the	DT
cord-016179-4i1n9j4x	63	3	RNA	RNA	NNP
cord-016179-4i1n9j4x	63	4	into	into	IN
cord-016179-4i1n9j4x	63	5	5	5	CD
cord-016179-4i1n9j4x	63	6	μL	μl	NN
cord-016179-4i1n9j4x	63	7	tube	tube	NN
cord-016179-4i1n9j4x	63	8	individually	individually	RB
cord-016179-4i1n9j4x	63	9	and	and	CC
cord-016179-4i1n9j4x	63	10	store	store	VB
cord-016179-4i1n9j4x	63	11	in	in	IN
cord-016179-4i1n9j4x	63	12	−80	−80	NNP
cord-016179-4i1n9j4x	63	13	°	°	NN
cord-016179-4i1n9j4x	63	14	C	c	NN
cord-016179-4i1n9j4x	63	15	freezer	freezer	NN
cord-016179-4i1n9j4x	63	16	for	for	IN
cord-016179-4i1n9j4x	63	17	use	use	NN
cord-016179-4i1n9j4x	63	18	in	in	IN
cord-016179-4i1n9j4x	63	19	real	real	JJ
cord-016179-4i1n9j4x	63	20	-	-	HYPH
cord-016179-4i1n9j4x	63	21	time	time	NN
cord-016179-4i1n9j4x	63	22	RT	RT	NNP
cord-016179-4i1n9j4x	63	23	-	-	HYPH
cord-016179-4i1n9j4x	63	24	PCR	PCR	NNP
cord-016179-4i1n9j4x	63	25	.	.	.
cord-016179-4i1n9j4x	64	1	1	1	LS
cord-016179-4i1n9j4x	64	2	.	.	.
cord-016179-4i1n9j4x	65	1	Prepare	prepare	VB
cord-016179-4i1n9j4x	65	2	a	a	DT
cord-016179-4i1n9j4x	65	3	total	total	NN
cord-016179-4i1n9j4x	65	4	of	of	IN
cord-016179-4i1n9j4x	65	5	25	25	CD
cord-016179-4i1n9j4x	65	6	μL	μL	NNS
cord-016179-4i1n9j4x	65	7	of	of	IN
cord-016179-4i1n9j4x	65	8	reaction	reaction	NN
cord-016179-4i1n9j4x	65	9	mixture	mixture	NN
cord-016179-4i1n9j4x	65	10	(	(	-LRB-
cord-016179-4i1n9j4x	65	11	Table	table	NN
cord-016179-4i1n9j4x	65	12	2	2	CD
cord-016179-4i1n9j4x	65	13	)	)	-RRB-
cord-016179-4i1n9j4x	65	14	on	on	IN
cord-016179-4i1n9j4x	65	15	ice	ice	NN
cord-016179-4i1n9j4x	65	16	by	by	IN
cord-016179-4i1n9j4x	65	17	using	use	VBG
cord-016179-4i1n9j4x	65	18	TCoV	tcov	NN
cord-016179-4i1n9j4x	65	19	-	-	HYPH
cord-016179-4i1n9j4x	65	20	specific	specific	JJ
cord-016179-4i1n9j4x	65	21	primers	primer	NNS
cord-016179-4i1n9j4x	65	22	,	,	,
cord-016179-4i1n9j4x	65	23	probe	probe	NN
cord-016179-4i1n9j4x	65	24	,	,	,
cord-016179-4i1n9j4x	65	25	reaction	reaction	NN
cord-016179-4i1n9j4x	65	26	buffer	buffer	NN
cord-016179-4i1n9j4x	65	27	from	from	IN
cord-016179-4i1n9j4x	65	28	Platinum	Platinum	NNP
cord-016179-4i1n9j4x	66	1	®	®	.
cord-016179-4i1n9j4x	66	2	Quantitative	Quantitative	NNP
cord-016179-4i1n9j4x	66	3	RT	RT	NNP
cord-016179-4i1n9j4x	66	4	-	-	HYPH
cord-016179-4i1n9j4x	66	5	PCR	PCR	NNP
cord-016179-4i1n9j4x	66	6	ThermoScript	ThermoScript	NNP
cord-016179-4i1n9j4x	66	7	™	™	CD
cord-016179-4i1n9j4x	66	8	One	one	CD
cord-016179-4i1n9j4x	66	9	-	-	HYPH
cord-016179-4i1n9j4x	66	10	Step	Step	NNP
cord-016179-4i1n9j4x	66	11	System	System	NNP
cord-016179-4i1n9j4x	66	12	,	,	,
cord-016179-4i1n9j4x	66	13	and	and	CC
cord-016179-4i1n9j4x	66	14	5	5	CD
cord-016179-4i1n9j4x	66	15	μL	μL	NNS
cord-016179-4i1n9j4x	66	16	of	of	IN
cord-016179-4i1n9j4x	66	17	RNA	rna	NN
cord-016179-4i1n9j4x	66	18	template	template	NN
cord-016179-4i1n9j4x	66	19	.	.	.
cord-016179-4i1n9j4x	67	1	Mix	mix	VB
cord-016179-4i1n9j4x	67	2	gently	gently	RB
cord-016179-4i1n9j4x	67	3	by	by	IN
cord-016179-4i1n9j4x	67	4	pipetting	pipette	VBG
cord-016179-4i1n9j4x	67	5	up	up	RP
cord-016179-4i1n9j4x	67	6	and	and	CC
cord-016179-4i1n9j4x	67	7	down	down	RB
cord-016179-4i1n9j4x	67	8	(	(	-LRB-
cord-016179-4i1n9j4x	67	9	see	see	VB
cord-016179-4i1n9j4x	67	10	Note	Note	NNP
cord-016179-4i1n9j4x	67	11	4	4	CD
cord-016179-4i1n9j4x	67	12	)	)	-RRB-
cord-016179-4i1n9j4x	67	13	.	.	.
cord-016179-4i1n9j4x	68	1	1	1	LS
cord-016179-4i1n9j4x	68	2	.	.	.
cord-016179-4i1n9j4x	69	1	Amplify	amplify	VB
cord-016179-4i1n9j4x	69	2	partial	partial	JJ
cord-016179-4i1n9j4x	69	3	S2	s2	NN
cord-016179-4i1n9j4x	69	4	gene	gene	NN
cord-016179-4i1n9j4x	69	5	of	of	IN
cord-016179-4i1n9j4x	69	6	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	69	7	in	in	IN
cord-016179-4i1n9j4x	69	8	the	the	DT
cord-016179-4i1n9j4x	69	9	region	region	NN
cord-016179-4i1n9j4x	69	10	encompassing	encompass	VBG
cord-016179-4i1n9j4x	69	11	the	the	DT
cord-016179-4i1n9j4x	69	12	fragment	fragment	NN
cord-016179-4i1n9j4x	69	13	targeted	target	VBN
cord-016179-4i1n9j4x	69	14	by	by	IN
cord-016179-4i1n9j4x	69	15	real	real	JJ
cord-016179-4i1n9j4x	69	16	-	-	HYPH
cord-016179-4i1n9j4x	69	17	time	time	NN
cord-016179-4i1n9j4x	69	18	RT	RT	NNP
cord-016179-4i1n9j4x	69	19	-	-	HYPH
cord-016179-4i1n9j4x	69	20	PCR	PCR	NNP
cord-016179-4i1n9j4x	69	21	by	by	IN
cord-016179-4i1n9j4x	69	22	PCR	PCR	NNP
cord-016179-4i1n9j4x	69	23	with	with	IN
cord-016179-4i1n9j4x	69	24	forward	forward	JJ
cord-016179-4i1n9j4x	69	25	primer	primer	NN
cord-016179-4i1n9j4x	69	26	6F	6F	NNP
cord-016179-4i1n9j4x	69	27	and	and	CC
cord-016179-4i1n9j4x	69	28	reverse	reverse	JJ
cord-016179-4i1n9j4x	69	29	primer	primer	NN
cord-016179-4i1n9j4x	69	30	6R	6r	NN
cord-016179-4i1n9j4x	69	31	(	(	-LRB-
cord-016179-4i1n9j4x	69	32	Table	Table	NNP
cord-016179-4i1n9j4x	69	33	1	1	CD
cord-016179-4i1n9j4x	69	34	)	)	-RRB-
cord-016179-4i1n9j4x	69	35	and	and	CC
cord-016179-4i1n9j4x	69	36	the	the	DT
cord-016179-4i1n9j4x	69	37	amplified	amplify	VBN
cord-016179-4i1n9j4x	69	38	fragment	fragment	NN
cord-016179-4i1n9j4x	69	39	is	be	VBZ
cord-016179-4i1n9j4x	69	40	designed	design	VBN
cord-016179-4i1n9j4x	69	41	as	as	IN
cord-016179-4i1n9j4x	69	42	6F/6R	6f/6r	CD
cord-016179-4i1n9j4x	69	43	fragment	fragment	NN
cord-016179-4i1n9j4x	69	44	.	.	.
cord-016179-4i1n9j4x	70	1	The	the	DT
cord-016179-4i1n9j4x	70	2	fragments	fragment	NNS
cord-016179-4i1n9j4x	70	3	underlined	underline	VBN
cord-016179-4i1n9j4x	70	4	in	in	IN
cord-016179-4i1n9j4x	70	5	primers	primer	NNS
cord-016179-4i1n9j4x	70	6	6F	6F	NNP
cord-016179-4i1n9j4x	70	7	and	and	CC
cord-016179-4i1n9j4x	70	8	6R	6R	NNP
cord-016179-4i1n9j4x	70	9	are	be	VBP
cord-016179-4i1n9j4x	70	10	recognized	recognize	VBN
cord-016179-4i1n9j4x	70	11	by	by	IN
cord-016179-4i1n9j4x	70	12	restriction	restriction	NN
cord-016179-4i1n9j4x	70	13	enzymes	enzyme	NNS
cord-016179-4i1n9j4x	70	14	NcoI	NcoI	NNP
cord-016179-4i1n9j4x	70	15	and	and	CC
cord-016179-4i1n9j4x	70	16	KpnI	KpnI	NNP
cord-016179-4i1n9j4x	70	17	,	,	,
cord-016179-4i1n9j4x	70	18	respectively	respectively	RB
cord-016179-4i1n9j4x	70	19	.	.	.
cord-016179-4i1n9j4x	71	1	2	2	LS
cord-016179-4i1n9j4x	71	2	.	.	.
cord-016179-4i1n9j4x	72	1	Double	double	JJ
cord-016179-4i1n9j4x	72	2	-	-	HYPH
cord-016179-4i1n9j4x	72	3	digest	digest	VB
cord-016179-4i1n9j4x	72	4	the	the	DT
cord-016179-4i1n9j4x	72	5	pTriEx-3	pTriEx-3	NNP
cord-016179-4i1n9j4x	72	6	DNA	DNA	NNP
cord-016179-4i1n9j4x	72	7	-	-	HYPH
cord-016179-4i1n9j4x	72	8	Novagen	Novagen	NNP
cord-016179-4i1n9j4x	72	9	(	(	-LRB-
cord-016179-4i1n9j4x	72	10	1	1	CD
cord-016179-4i1n9j4x	72	11	-	-	SYM
cord-016179-4i1n9j4x	72	12	3	3	CD
cord-016179-4i1n9j4x	72	13	μg	μg	NNP
cord-016179-4i1n9j4x	72	14	total	total	NN
cord-016179-4i1n9j4x	72	15	)	)	-RRB-
cord-016179-4i1n9j4x	72	16	or	or	CC
cord-016179-4i1n9j4x	72	17	6F/6R	6f/6r	CD
cord-016179-4i1n9j4x	72	18	fragment	fragment	NN
cord-016179-4i1n9j4x	72	19	(	(	-LRB-
cord-016179-4i1n9j4x	72	20	1	1	CD
cord-016179-4i1n9j4x	72	21	-	-	SYM
cord-016179-4i1n9j4x	72	22	3	3	CD
cord-016179-4i1n9j4x	72	23	μg	μg	NNP
cord-016179-4i1n9j4x	72	24	total	total	NN
cord-016179-4i1n9j4x	72	25	)	)	-RRB-
cord-016179-4i1n9j4x	72	26	with	with	IN
cord-016179-4i1n9j4x	72	27	1	1	CD
cord-016179-4i1n9j4x	72	28	μL	μL	NNS
cord-016179-4i1n9j4x	72	29	of	of	IN
cord-016179-4i1n9j4x	72	30	1/10	1/10	CD
cord-016179-4i1n9j4x	72	31	diluted	dilute	VBN
cord-016179-4i1n9j4x	72	32	NcoI	NcoI	NNP
cord-016179-4i1n9j4x	72	33	and	and	CC
cord-016179-4i1n9j4x	72	34	1	1	CD
cord-016179-4i1n9j4x	72	35	μL	μL	NNS
cord-016179-4i1n9j4x	72	36	of	of	IN
cord-016179-4i1n9j4x	72	37	1/10	1/10	CD
cord-016179-4i1n9j4x	72	38	diluted	dilute	VBN
cord-016179-4i1n9j4x	72	39	KpnI	KpnI	NNP
cord-016179-4i1n9j4x	72	40	(	(	-LRB-
cord-016179-4i1n9j4x	72	41	NEB	NEB	NNP
cord-016179-4i1n9j4x	72	42	)	)	-RRB-
cord-016179-4i1n9j4x	72	43	in	in	IN
cord-016179-4i1n9j4x	72	44	50	50	CD
cord-016179-4i1n9j4x	72	45	μL	μL	NNP
cord-016179-4i1n9j4x	72	46	reaction	reaction	NN
cord-016179-4i1n9j4x	72	47	containing	contain	VBG
cord-016179-4i1n9j4x	72	48	5	5	CD
cord-016179-4i1n9j4x	72	49	μL	μl	NN
cord-016179-4i1n9j4x	72	50	of	of	IN
cord-016179-4i1n9j4x	72	51	10×	10×	CD
cord-016179-4i1n9j4x	72	52	NEB	NEB	NNP
cord-016179-4i1n9j4x	72	53	®	®	NNP
cord-016179-4i1n9j4x	72	54	Buffer	Buffer	NNP
cord-016179-4i1n9j4x	72	55	1	1	CD
cord-016179-4i1n9j4x	72	56	and	and	CC
cord-016179-4i1n9j4x	72	57	5	5	CD
cord-016179-4i1n9j4x	72	58	μL	μL	NNS
cord-016179-4i1n9j4x	72	59	of	of	IN
cord-016179-4i1n9j4x	72	60	bovine	bovine	JJ
cord-016179-4i1n9j4x	72	61	serum	serum	NN
cord-016179-4i1n9j4x	72	62	albumin	albumin	NN
cord-016179-4i1n9j4x	72	63	(	(	-LRB-
cord-016179-4i1n9j4x	72	64	BSA	BSA	NNP
cord-016179-4i1n9j4x	72	65	,	,	,
cord-016179-4i1n9j4x	72	66	1	1	CD
cord-016179-4i1n9j4x	72	67	mg	mg	NNP
cord-016179-4i1n9j4x	72	68	/	/	SYM
cord-016179-4i1n9j4x	72	69	mL	mL	NNP
cord-016179-4i1n9j4x	72	70	)	)	-RRB-
cord-016179-4i1n9j4x	72	71	.	.	.
cord-016179-4i1n9j4x	73	1	Incubate	incubate	VB
cord-016179-4i1n9j4x	73	2	the	the	DT
cord-016179-4i1n9j4x	73	3	reaction	reaction	NN
cord-016179-4i1n9j4x	73	4	at	at	IN
cord-016179-4i1n9j4x	73	5	37	37	CD
cord-016179-4i1n9j4x	73	6	°	°	NN
cord-016179-4i1n9j4x	73	7	C	c	NN
cord-016179-4i1n9j4x	73	8	for	for	IN
cord-016179-4i1n9j4x	73	9	1	1	CD
cord-016179-4i1n9j4x	73	10	h.	h.	NN
cord-016179-4i1n9j4x	73	11	3	3	CD
cord-016179-4i1n9j4x	73	12	.	.	.
cord-016179-4i1n9j4x	74	1	Ligate	Ligate	NNP
cord-016179-4i1n9j4x	74	2	the	the	DT
cord-016179-4i1n9j4x	74	3	treated	treat	VBN
cord-016179-4i1n9j4x	74	4	pTriEx-3	pTriEx-3	NNP
cord-016179-4i1n9j4x	74	5	and	and	CC
cord-016179-4i1n9j4x	74	6	6	6	CD
cord-016179-4i1n9j4x	74	7	F/6R	f/6r	NN
cord-016179-4i1n9j4x	74	8	fragment	fragment	NN
cord-016179-4i1n9j4x	74	9	(	(	-LRB-
cord-016179-4i1n9j4x	74	10	638	638	CD
cord-016179-4i1n9j4x	74	11	bp	bp	NNP
cord-016179-4i1n9j4x	74	12	)	)	-RRB-
cord-016179-4i1n9j4x	74	13	using	use	VBG
cord-016179-4i1n9j4x	74	14	T4	T4	NNP
cord-016179-4i1n9j4x	74	15	DNA	DNA	NNP
cord-016179-4i1n9j4x	74	16	ligase	ligase	NN
cord-016179-4i1n9j4x	74	17	in	in	IN
cord-016179-4i1n9j4x	74	18	20	20	CD
cord-016179-4i1n9j4x	74	19	μL	μl	NN
cord-016179-4i1n9j4x	74	20	reaction	reaction	NN
cord-016179-4i1n9j4x	74	21	to	to	TO
cord-016179-4i1n9j4x	74	22	become	become	VB
cord-016179-4i1n9j4x	74	23	pTriEx3	pTriEx3	NNP
cord-016179-4i1n9j4x	74	24	-	-	HYPH
cord-016179-4i1n9j4x	74	25	6F/6R.	6F/6R.	NNP
cord-016179-4i1n9j4x	75	1	The	the	DT
cord-016179-4i1n9j4x	75	2	reaction	reaction	NN
cord-016179-4i1n9j4x	75	3	solution	solution	NN
cord-016179-4i1n9j4x	75	4	contains	contain	VBZ
cord-016179-4i1n9j4x	75	5	total	total	JJ
cord-016179-4i1n9j4x	75	6	64	64	CD
cord-016179-4i1n9j4x	75	7	ng	ng	NN
cord-016179-4i1n9j4x	75	8	of	of	IN
cord-016179-4i1n9j4x	75	9	the	the	DT
cord-016179-4i1n9j4x	75	10	10	10	CD
cord-016179-4i1n9j4x	75	11	6	6	CD
cord-016179-4i1n9j4x	75	12	023	023	CD
cord-016179-4i1n9j4x	75	13	10	10	CD
cord-016179-4i1n9j4x	75	14	9	9	CD
cord-016179-4i1n9j4x	75	15	2	2	CD
cord-016179-4i1n9j4x	75	16	3	3	CD
cord-016179-4i1n9j4x	75	17	1	1	CD
cord-016179-4i1n9j4x	75	18	.	.	.
cord-016179-4i1n9j4x	76	1	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	76	2	stabilization	stabilization	NN
cord-016179-4i1n9j4x	76	3	reagent	reagent	NN
cord-016179-4i1n9j4x	76	4	is	be	VBZ
cord-016179-4i1n9j4x	76	5	not	not	RB
cord-016179-4i1n9j4x	76	6	designed	design	VBN
cord-016179-4i1n9j4x	76	7	for	for	IN
cord-016179-4i1n9j4x	76	8	fecal	fecal	JJ
cord-016179-4i1n9j4x	76	9	samples	sample	NNS
cord-016179-4i1n9j4x	76	10	but	but	CC
cord-016179-4i1n9j4x	76	11	the	the	DT
cord-016179-4i1n9j4x	76	12	previous	previous	JJ
cord-016179-4i1n9j4x	76	13	study	study	NN
cord-016179-4i1n9j4x	76	14	[	[	-LRB-
cord-016179-4i1n9j4x	76	15	10	10	CD
cord-016179-4i1n9j4x	76	16	]	]	-RRB-
cord-016179-4i1n9j4x	76	17	showed	show	VBD
cord-016179-4i1n9j4x	76	18	a	a	DT
cord-016179-4i1n9j4x	76	19	good	good	JJ
cord-016179-4i1n9j4x	76	20	stability	stability	NN
cord-016179-4i1n9j4x	76	21	of	of	IN
cord-016179-4i1n9j4x	76	22	viral	viral	JJ
cord-016179-4i1n9j4x	76	23	RNA	RNA	NNP
cord-016179-4i1n9j4x	76	24	in	in	IN
cord-016179-4i1n9j4x	76	25	turkey	turkey	NN
cord-016179-4i1n9j4x	76	26	feces	fece	NNS
cord-016179-4i1n9j4x	76	27	by	by	IN
cord-016179-4i1n9j4x	76	28	using	use	VBG
cord-016179-4i1n9j4x	76	29	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	76	30	reagent	reagent	NN
cord-016179-4i1n9j4x	76	31	.	.	.
cord-016179-4i1n9j4x	77	1	It	-PRON-	PRP
cord-016179-4i1n9j4x	77	2	is	be	VBZ
cord-016179-4i1n9j4x	77	3	very	very	RB
cord-016179-4i1n9j4x	77	4	critical	critical	JJ
cord-016179-4i1n9j4x	77	5	to	to	TO
cord-016179-4i1n9j4x	77	6	mix	mix	VB
cord-016179-4i1n9j4x	77	7	feces	fece	NNS
cord-016179-4i1n9j4x	77	8	or	or	CC
cord-016179-4i1n9j4x	77	9	cloacal	cloacal	JJ
cord-016179-4i1n9j4x	77	10	swab	swab	NN
cord-016179-4i1n9j4x	77	11	with	with	IN
cord-016179-4i1n9j4x	77	12	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	77	13	to	to	TO
cord-016179-4i1n9j4x	77	14	make	make	VB
cord-016179-4i1n9j4x	77	15	feces	fece	NNS
cord-016179-4i1n9j4x	77	16	submerged	submerge	VBN
cord-016179-4i1n9j4x	77	17	and	and	CC
cord-016179-4i1n9j4x	77	18	contact	contact	NN
cord-016179-4i1n9j4x	77	19	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	77	20	reagent	reagent	NN
cord-016179-4i1n9j4x	77	21	completely	completely	RB
cord-016179-4i1n9j4x	77	22	for	for	IN
cord-016179-4i1n9j4x	77	23	the	the	DT
cord-016179-4i1n9j4x	77	24	stability	stability	NN
cord-016179-4i1n9j4x	77	25	of	of	IN
cord-016179-4i1n9j4x	77	26	RNA	RNA	NNP
cord-016179-4i1n9j4x	77	27	in	in	IN
cord-016179-4i1n9j4x	77	28	feces	fece	NNS
cord-016179-4i1n9j4x	77	29	.	.	.
cord-016179-4i1n9j4x	78	1	2	2	LS
cord-016179-4i1n9j4x	78	2	.	.	.
cord-016179-4i1n9j4x	79	1	The	the	DT
cord-016179-4i1n9j4x	79	2	thickness	thickness	NN
cord-016179-4i1n9j4x	79	3	of	of	IN
cord-016179-4i1n9j4x	79	4	tissue	tissue	NN
cord-016179-4i1n9j4x	79	5	must	must	MD
cord-016179-4i1n9j4x	79	6	be	be	VB
cord-016179-4i1n9j4x	79	7	less	less	JJR
cord-016179-4i1n9j4x	79	8	than	than	IN
cord-016179-4i1n9j4x	79	9	0.5	0.5	CD
cord-016179-4i1n9j4x	79	10	cm	cm	NNS
cord-016179-4i1n9j4x	79	11	.	.	.
cord-016179-4i1n9j4x	80	1	RNA	RNA	NNP
cord-016179-4i1n9j4x	80	2	in	in	IN
cord-016179-4i1n9j4x	80	3	harvested	harvest	VBN
cord-016179-4i1n9j4x	80	4	intestine	intestine	NN
cord-016179-4i1n9j4x	80	5	tissue	tissue	NN
cord-016179-4i1n9j4x	80	6	is	be	VBZ
cord-016179-4i1n9j4x	80	7	not	not	RB
cord-016179-4i1n9j4x	80	8	protected	protect	VBN
cord-016179-4i1n9j4x	80	9	until	until	IN
cord-016179-4i1n9j4x	80	10	the	the	DT
cord-016179-4i1n9j4x	80	11	tissue	tissue	NN
cord-016179-4i1n9j4x	80	12	is	be	VBZ
cord-016179-4i1n9j4x	80	13	completely	completely	RB
cord-016179-4i1n9j4x	80	14	submerged	submerge	VBN
cord-016179-4i1n9j4x	80	15	in	in	IN
cord-016179-4i1n9j4x	80	16	a	a	DT
cord-016179-4i1n9j4x	80	17	sufficient	sufficient	JJ
cord-016179-4i1n9j4x	80	18	volume	volume	NN
cord-016179-4i1n9j4x	80	19	of	of	IN
cord-016179-4i1n9j4x	80	20	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	80	21	RNA	RNA	NNP
cord-016179-4i1n9j4x	80	22	Stabilization	Stabilization	NNP
cord-016179-4i1n9j4x	80	23	Reagent	Reagent	NNP
cord-016179-4i1n9j4x	80	24	at	at	IN
cord-016179-4i1n9j4x	80	25	about	about	RB
cord-016179-4i1n9j4x	80	26	10	10	CD
cord-016179-4i1n9j4x	80	27	μL	μl	NN
cord-016179-4i1n9j4x	80	28	reagent	reagent	NN
cord-016179-4i1n9j4x	80	29	per	per	IN
cord-016179-4i1n9j4x	80	30	1	1	CD
cord-016179-4i1n9j4x	80	31	mg	mg	CD
cord-016179-4i1n9j4x	80	32	tissue	tissue	NN
cord-016179-4i1n9j4x	80	33	.	.	.
cord-016179-4i1n9j4x	81	1	The	the	DT
cord-016179-4i1n9j4x	81	2	intestinal	intestinal	JJ
cord-016179-4i1n9j4x	81	3	content	content	NN
cord-016179-4i1n9j4x	81	4	may	may	MD
cord-016179-4i1n9j4x	81	5	interfere	interfere	VB
cord-016179-4i1n9j4x	81	6	with	with	IN
cord-016179-4i1n9j4x	81	7	the	the	DT
cord-016179-4i1n9j4x	81	8	reaction	reaction	NN
cord-016179-4i1n9j4x	81	9	of	of	IN
cord-016179-4i1n9j4x	81	10	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	81	11	reagent	reagent	NN
cord-016179-4i1n9j4x	81	12	,	,	,
cord-016179-4i1n9j4x	81	13	so	so	CC
cord-016179-4i1n9j4x	81	14	it	-PRON-	PRP
cord-016179-4i1n9j4x	81	15	is	be	VBZ
cord-016179-4i1n9j4x	81	16	critical	critical	JJ
cord-016179-4i1n9j4x	81	17	to	to	TO
cord-016179-4i1n9j4x	81	18	wash	wash	VB
cord-016179-4i1n9j4x	81	19	off	off	RP
cord-016179-4i1n9j4x	81	20	the	the	DT
cord-016179-4i1n9j4x	81	21	intestinal	intestinal	JJ
cord-016179-4i1n9j4x	81	22	contents	content	NNS
cord-016179-4i1n9j4x	81	23	.	.	.
cord-016179-4i1n9j4x	82	1	Insufficient	Insufficient	NNP
cord-016179-4i1n9j4x	82	2	RNA	RNA	NNP
cord-016179-4i1n9j4x	82	3	reagent	reagent	NN
cord-016179-4i1n9j4x	82	4	may	may	MD
cord-016179-4i1n9j4x	82	5	cause	cause	VB
cord-016179-4i1n9j4x	82	6	RNA	RNA	NNP
cord-016179-4i1n9j4x	82	7	degradation	degradation	NN
cord-016179-4i1n9j4x	82	8	during	during	IN
cord-016179-4i1n9j4x	82	9	storage	storage	NN
cord-016179-4i1n9j4x	82	10	.	.	.
cord-016179-4i1n9j4x	83	1	It	-PRON-	PRP
cord-016179-4i1n9j4x	83	2	is	be	VBZ
cord-016179-4i1n9j4x	83	3	not	not	RB
cord-016179-4i1n9j4x	83	4	recommended	recommend	VBN
cord-016179-4i1n9j4x	83	5	to	to	TO
cord-016179-4i1n9j4x	83	6	harvest	harvest	VB
cord-016179-4i1n9j4x	83	7	tissues	tissue	NNS
cord-016179-4i1n9j4x	83	8	frozen	freeze	VBN
cord-016179-4i1n9j4x	83	9	in	in	IN
cord-016179-4i1n9j4x	83	10	liquid	liquid	JJ
cord-016179-4i1n9j4x	83	11	nitrogen	nitrogen	NN
cord-016179-4i1n9j4x	83	12	or	or	CC
cord-016179-4i1n9j4x	83	13	dry	dry	JJ
cord-016179-4i1n9j4x	83	14	ice	ice	NN
cord-016179-4i1n9j4x	83	15	and	and	CC
cord-016179-4i1n9j4x	83	16	later	later	RB
cord-016179-4i1n9j4x	83	17	thaw	thaw	VB
cord-016179-4i1n9j4x	83	18	them	-PRON-	PRP
cord-016179-4i1n9j4x	83	19	for	for	IN
cord-016179-4i1n9j4x	83	20	RNAlater	RNAlater	NNP
cord-016179-4i1n9j4x	83	21	storage	storage	NN
cord-016179-4i1n9j4x	83	22	or	or	CC
cord-016179-4i1n9j4x	83	23	RNA	RNA	NNP
cord-016179-4i1n9j4x	83	24	extraction	extraction	NN
cord-016179-4i1n9j4x	83	25	because	because	IN
cord-016179-4i1n9j4x	83	26	the	the	DT
cord-016179-4i1n9j4x	83	27	process	process	NN
cord-016179-4i1n9j4x	83	28	would	would	MD
cord-016179-4i1n9j4x	83	29	cause	cause	VB
cord-016179-4i1n9j4x	83	30	severe	severe	JJ
cord-016179-4i1n9j4x	83	31	RNA	RNA	NNP
cord-016179-4i1n9j4x	83	32	degradation	degradation	NN
cord-016179-4i1n9j4x	83	33	.	.	.
cord-016179-4i1n9j4x	84	1	3	3	LS
cord-016179-4i1n9j4x	84	2	.	.	.
cord-016179-4i1n9j4x	85	1	The	the	DT
cord-016179-4i1n9j4x	85	2	heat	heat	NN
cord-016179-4i1n9j4x	85	3	produced	produce	VBN
cord-016179-4i1n9j4x	85	4	by	by	IN
cord-016179-4i1n9j4x	85	5	the	the	DT
cord-016179-4i1n9j4x	85	6	process	process	NN
cord-016179-4i1n9j4x	85	7	of	of	IN
cord-016179-4i1n9j4x	85	8	hominization	hominization	NN
cord-016179-4i1n9j4x	85	9	can	can	MD
cord-016179-4i1n9j4x	85	10	damage	damage	VB
cord-016179-4i1n9j4x	85	11	the	the	DT
cord-016179-4i1n9j4x	85	12	integrity	integrity	NN
cord-016179-4i1n9j4x	85	13	of	of	IN
cord-016179-4i1n9j4x	85	14	RNA	RNA	NNP
cord-016179-4i1n9j4x	85	15	in	in	IN
cord-016179-4i1n9j4x	85	16	intestine	intestine	NN
cord-016179-4i1n9j4x	85	17	tissues	tissue	NNS
cord-016179-4i1n9j4x	85	18	leading	lead	VBG
cord-016179-4i1n9j4x	85	19	to	to	IN
cord-016179-4i1n9j4x	85	20	low	low	JJ
cord-016179-4i1n9j4x	85	21	sensitivity	sensitivity	NN
cord-016179-4i1n9j4x	85	22	of	of	IN
cord-016179-4i1n9j4x	85	23	real	real	JJ
cord-016179-4i1n9j4x	85	24	-	-	HYPH
cord-016179-4i1n9j4x	85	25	time	time	NN
cord-016179-4i1n9j4x	85	26	RT	RT	NNP
cord-016179-4i1n9j4x	85	27	-	-	HYPH
cord-016179-4i1n9j4x	85	28	PCR	PCR	NNP
cord-016179-4i1n9j4x	85	29	.	.	.
cord-016179-4i1n9j4x	86	1	Therefore	therefore	RB
cord-016179-4i1n9j4x	86	2	,	,	,
cord-016179-4i1n9j4x	86	3	it	-PRON-	PRP
cord-016179-4i1n9j4x	86	4	is	be	VBZ
cord-016179-4i1n9j4x	86	5	recommended	recommend	VBN
cord-016179-4i1n9j4x	86	6	to	to	TO
cord-016179-4i1n9j4x	86	7	homogenize	homogenize	VB
cord-016179-4i1n9j4x	86	8	the	the	DT
cord-016179-4i1n9j4x	86	9	intestine	intestine	NN
cord-016179-4i1n9j4x	86	10	tissue	tissue	NN
cord-016179-4i1n9j4x	86	11	for	for	IN
cord-016179-4i1n9j4x	86	12	30	30	CD
cord-016179-4i1n9j4x	86	13	s	s	NN
cord-016179-4i1n9j4x	86	14	and	and	CC
cord-016179-4i1n9j4x	86	15	cool	cool	VB
cord-016179-4i1n9j4x	86	16	the	the	DT
cord-016179-4i1n9j4x	86	17	tube	tube	NN
cord-016179-4i1n9j4x	86	18	in	in	IN
cord-016179-4i1n9j4x	86	19	ice	ice	NN
cord-016179-4i1n9j4x	86	20	in	in	IN
cord-016179-4i1n9j4x	86	21	cycles	cycle	NNS
cord-016179-4i1n9j4x	86	22	until	until	IN
cord-016179-4i1n9j4x	86	23	the	the	DT
cord-016179-4i1n9j4x	86	24	intestine	intestine	NN
cord-016179-4i1n9j4x	86	25	tissue	tissue	NN
cord-016179-4i1n9j4x	86	26	is	be	VBZ
cord-016179-4i1n9j4x	86	27	homogenized	homogenize	VBN
cord-016179-4i1n9j4x	86	28	thoroughly	thoroughly	RB
cord-016179-4i1n9j4x	86	29	.	.	.
cord-016179-4i1n9j4x	87	1	4	4	LS
cord-016179-4i1n9j4x	87	2	.	.	.
cord-016179-4i1n9j4x	88	1	Real	real	JJ
cord-016179-4i1n9j4x	88	2	-	-	HYPH
cord-016179-4i1n9j4x	88	3	time	time	NN
cord-016179-4i1n9j4x	88	4	RT	RT	NNP
cord-016179-4i1n9j4x	88	5	-	-	HYPH
cord-016179-4i1n9j4x	88	6	PCR	PCR	NNP
cord-016179-4i1n9j4x	88	7	is	be	VBZ
cord-016179-4i1n9j4x	88	8	a	a	DT
cord-016179-4i1n9j4x	88	9	very	very	RB
cord-016179-4i1n9j4x	88	10	sensitive	sensitive	JJ
cord-016179-4i1n9j4x	88	11	assay	assay	NN
cord-016179-4i1n9j4x	88	12	,	,	,
cord-016179-4i1n9j4x	88	13	so	so	CC
cord-016179-4i1n9j4x	88	14	it	-PRON-	PRP
cord-016179-4i1n9j4x	88	15	is	be	VBZ
cord-016179-4i1n9j4x	88	16	very	very	RB
cord-016179-4i1n9j4x	88	17	easy	easy	JJ
cord-016179-4i1n9j4x	88	18	to	to	TO
cord-016179-4i1n9j4x	88	19	be	be	VB
cord-016179-4i1n9j4x	88	20	interfered	interfere	VBN
cord-016179-4i1n9j4x	88	21	by	by	IN
cord-016179-4i1n9j4x	88	22	bubbles	bubble	NNS
cord-016179-4i1n9j4x	88	23	created	create	VBN
cord-016179-4i1n9j4x	88	24	by	by	IN
cord-016179-4i1n9j4x	88	25	pipetting	pipette	VBG
cord-016179-4i1n9j4x	88	26	.	.	.
cord-016179-4i1n9j4x	89	1	To	to	TO
cord-016179-4i1n9j4x	89	2	use	use	VB
cord-016179-4i1n9j4x	89	3	pipettes	pipette	NNS
cord-016179-4i1n9j4x	89	4	and	and	CC
cord-016179-4i1n9j4x	89	5	take	take	VB
cord-016179-4i1n9j4x	89	6	small	small	JJ
cord-016179-4i1n9j4x	89	7	volume	volume	NN
cord-016179-4i1n9j4x	89	8	of	of	IN
cord-016179-4i1n9j4x	89	9	reagents	reagent	NNS
cord-016179-4i1n9j4x	89	10	(	(	-LRB-
cord-016179-4i1n9j4x	89	11	<	<	XX
cord-016179-4i1n9j4x	89	12	10	10	CD
cord-016179-4i1n9j4x	89	13	μL	μL	NNS
cord-016179-4i1n9j4x	89	14	)	)	-RRB-
cord-016179-4i1n9j4x	90	1	precisely	precisely	RB
cord-016179-4i1n9j4x	90	2	,	,	,
cord-016179-4i1n9j4x	90	3	it	-PRON-	PRP
cord-016179-4i1n9j4x	90	4	is	be	VBZ
cord-016179-4i1n9j4x	90	5	recommended	recommend	VBN
cord-016179-4i1n9j4x	90	6	to	to	TO
cord-016179-4i1n9j4x	90	7	practice	practice	VB
cord-016179-4i1n9j4x	90	8	before	before	IN
cord-016179-4i1n9j4x	90	9	performing	perform	VBG
cord-016179-4i1n9j4x	90	10	the	the	DT
cord-016179-4i1n9j4x	90	11	real	real	JJ
cord-016179-4i1n9j4x	90	12	test	test	NN
cord-016179-4i1n9j4x	90	13	.	.	.
cord-016179-4i1n9j4x	91	1	In	in	IN
cord-016179-4i1n9j4x	91	2	addition	addition	NN
cord-016179-4i1n9j4x	91	3	,	,	,
cord-016179-4i1n9j4x	91	4	the	the	DT
cord-016179-4i1n9j4x	91	5	nonspecific	nonspecific	JJ
cord-016179-4i1n9j4x	91	6	reactions	reaction	NNS
cord-016179-4i1n9j4x	91	7	can	can	MD
cord-016179-4i1n9j4x	91	8	be	be	VB
cord-016179-4i1n9j4x	91	9	minimized	minimize	VBN
cord-016179-4i1n9j4x	91	10	by	by	IN
cord-016179-4i1n9j4x	91	11	mixing	mix	VBG
cord-016179-4i1n9j4x	91	12	reagents	reagent	NNS
cord-016179-4i1n9j4x	91	13	on	on	IN
cord-016179-4i1n9j4x	91	14	the	the	DT
cord-016179-4i1n9j4x	91	15	precooled	precooled	JJ
cord-016179-4i1n9j4x	91	16	(	(	-LRB-
cord-016179-4i1n9j4x	91	17	store	store	NN
cord-016179-4i1n9j4x	91	18	at	at	IN
cord-016179-4i1n9j4x	91	19	−20	−20	NNP
cord-016179-4i1n9j4x	91	20	°	°	NNP
cord-016179-4i1n9j4x	91	21	C	C	NNP
cord-016179-4i1n9j4x	91	22	freezer	freezer	NN
cord-016179-4i1n9j4x	91	23	)	)	-RRB-
cord-016179-4i1n9j4x	91	24	aluminum	aluminum	NN
cord-016179-4i1n9j4x	91	25	loading	loading	NN
cord-016179-4i1n9j4x	91	26	block	block	NN
cord-016179-4i1n9j4x	91	27	(	(	-LRB-
cord-016179-4i1n9j4x	91	28	Qiagen	Qiagen	NNP
cord-016179-4i1n9j4x	91	29	)	)	-RRB-
cord-016179-4i1n9j4x	91	30	on	on	IN
cord-016179-4i1n9j4x	91	31	ice	ice	NN
cord-016179-4i1n9j4x	91	32	.	.	.
cord-016179-4i1n9j4x	92	1	5	5	LS
cord-016179-4i1n9j4x	92	2	.	.	.
cord-016179-4i1n9j4x	93	1	Example	example	NN
cord-016179-4i1n9j4x	93	2	of	of	IN
cord-016179-4i1n9j4x	93	3	running	run	VBG
cord-016179-4i1n9j4x	93	4	a	a	DT
cord-016179-4i1n9j4x	93	5	set	set	NN
cord-016179-4i1n9j4x	93	6	of	of	IN
cord-016179-4i1n9j4x	93	7	samples	sample	NNS
cord-016179-4i1n9j4x	93	8	from	from	IN
cord-016179-4i1n9j4x	93	9	the	the	DT
cord-016179-4i1n9j4x	93	10	setup	setup	NN
cord-016179-4i1n9j4x	93	11	to	to	IN
cord-016179-4i1n9j4x	93	12	the	the	DT
cord-016179-4i1n9j4x	93	13	end	end	NN
cord-016179-4i1n9j4x	93	14	of	of	IN
cord-016179-4i1n9j4x	93	15	calculating	calculate	VBG
cord-016179-4i1n9j4x	93	16	the	the	DT
cord-016179-4i1n9j4x	93	17	copy	copy	NN
cord-016179-4i1n9j4x	93	18	number	number	NN
cord-016179-4i1n9j4x	93	19	is	be	VBZ
cord-016179-4i1n9j4x	93	20	illustrated	illustrate	VBN
cord-016179-4i1n9j4x	93	21	below	below	IN
cord-016179-4i1n9j4x	93	22	.	.	.
cord-016179-4i1n9j4x	94	1	First	first	RB
cord-016179-4i1n9j4x	94	2	,	,	,
cord-016179-4i1n9j4x	94	3	collect	collect	VB
cord-016179-4i1n9j4x	94	4	ileum	ileum	NN
cord-016179-4i1n9j4x	94	5	and	and	CC
cord-016179-4i1n9j4x	94	6	cecum	cecum	NN
cord-016179-4i1n9j4x	94	7	samples	sample	NNS
cord-016179-4i1n9j4x	94	8	in	in	IN
cord-016179-4i1n9j4x	94	9	RNAlater	rnalater	NN
cord-016179-4i1n9j4x	94	10	solution	solution	NN
cord-016179-4i1n9j4x	94	11	from	from	IN
cord-016179-4i1n9j4x	94	12	three	three	CD
cord-016179-4i1n9j4x	94	13	turkeys	turkey	NNS
cord-016179-4i1n9j4x	94	14	at	at	IN
cord-016179-4i1n9j4x	94	15	5	5	CD
cord-016179-4i1n9j4x	94	16	days	day	NNS
cord-016179-4i1n9j4x	94	17	post	post	NN
cord-016179-4i1n9j4x	94	18	-	-	NN
cord-016179-4i1n9j4x	94	19	infection	infection	NN
cord-016179-4i1n9j4x	94	20	of	of	IN
cord-016179-4i1n9j4x	94	21	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	94	22	and	and	CC
cord-016179-4i1n9j4x	94	23	the	the	DT
cord-016179-4i1n9j4x	94	24	same	same	JJ
cord-016179-4i1n9j4x	94	25	kind	kind	NN
cord-016179-4i1n9j4x	94	26	of	of	IN
cord-016179-4i1n9j4x	94	27	samples	sample	NNS
cord-016179-4i1n9j4x	94	28	from	from	IN
cord-016179-4i1n9j4x	94	29	negative	negative	JJ
cord-016179-4i1n9j4x	94	30	control	control	NN
cord-016179-4i1n9j4x	94	31	turkeys	turkey	NNS
cord-016179-4i1n9j4x	94	32	without	without	IN
cord-016179-4i1n9j4x	94	33	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	94	34	infection	infection	NN
cord-016179-4i1n9j4x	94	35	.	.	.
cord-016179-4i1n9j4x	95	1	Second	second	RB
cord-016179-4i1n9j4x	95	2	,	,	,
cord-016179-4i1n9j4x	95	3	label	label	VB
cord-016179-4i1n9j4x	95	4	the	the	DT
cord-016179-4i1n9j4x	95	5	samples	sample	NNS
cord-016179-4i1n9j4x	95	6	from	from	IN
cord-016179-4i1n9j4x	95	7	turkeys	turkey	NNS
cord-016179-4i1n9j4x	95	8	infected	infect	VBN
cord-016179-4i1n9j4x	95	9	with	with	IN
cord-016179-4i1n9j4x	95	10	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	95	11	as	as	IN
cord-016179-4i1n9j4x	95	12	d5-i1	d5-i1	NNP
cord-016179-4i1n9j4x	95	13	to	to	IN
cord-016179-4i1n9j4x	95	14	d5-i3	d5-i3	NNS
cord-016179-4i1n9j4x	95	15	and	and	CC
cord-016179-4i1n9j4x	95	16	d5-c1to	d5-c1to	NNP
cord-016179-4i1n9j4x	95	17	d5-c3	d5-c3	NNP
cord-016179-4i1n9j4x	95	18	,	,	,
cord-016179-4i1n9j4x	95	19	and	and	CC
cord-016179-4i1n9j4x	95	20	those	those	DT
cord-016179-4i1n9j4x	95	21	from	from	IN
cord-016179-4i1n9j4x	95	22	negative	negative	JJ
cord-016179-4i1n9j4x	95	23	control	control	NN
cord-016179-4i1n9j4x	95	24	turkeys	turkey	NNS
cord-016179-4i1n9j4x	95	25	without	without	IN
cord-016179-4i1n9j4x	95	26	TCoV	TCoV	NNP
cord-016179-4i1n9j4x	95	27	infection	infection	NN
cord-016179-4i1n9j4x	95	28	as	as	IN
cord-016179-4i1n9j4x	95	29	N	n	CD
cord-016179-4i1n9j4x	95	30	-	-	NN
cord-016179-4i1n9j4x	95	31	i1	i1	NN
cord-016179-4i1n9j4x	95	32	to	to	IN
cord-016179-4i1n9j4x	95	33	N	N	NNP
cord-016179-4i1n9j4x	95	34	-	-	HYPH
cord-016179-4i1n9j4x	95	35	i3	i3	NNP
cord-016179-4i1n9j4x	95	36	and	and	CC
cord-016179-4i1n9j4x	95	37	N	N	NNP
cord-016179-4i1n9j4x	95	38	-	-	HYPH
cord-016179-4i1n9j4x	95	39	c1	c1	NNP
cord-016179-4i1n9j4x	95	40	to	to	IN
cord-016179-4i1n9j4x	95	41	N	N	NNP
cord-016179-4i1n9j4x	95	42	-	-	HYPH
cord-016179-4i1n9j4x	95	43	c3	c3	NN
cord-016179-4i1n9j4x	95	44	.	.	.
cord-016179-4i1n9j4x	96	1	Third	third	RB
cord-016179-4i1n9j4x	96	2	,	,	,
cord-016179-4i1n9j4x	96	3	extract	extract	VB
cord-016179-4i1n9j4x	96	4	RNA	RNA	NNP
cord-016179-4i1n9j4x	96	5	from	from	IN
cord-016179-4i1n9j4x	96	6	the	the	DT
cord-016179-4i1n9j4x	96	7	samples	sample	NNS
cord-016179-4i1n9j4x	96	8	according	accord	VBG
cord-016179-4i1n9j4x	96	9	to	to	IN
cord-016179-4i1n9j4x	96	10	Sect	Sect	NNP
cord-016179-4i1n9j4x	96	11	.	.	.
cord-016179-4i1n9j4x	97	1	3.2.2	3.2.2	NNP
cord-016179-4i1n9j4x	97	2	.	.	.
cord-016179-4i1n9j4x	98	1	Next	next	RB
cord-016179-4i1n9j4x	98	2	,	,	,
cord-016179-4i1n9j4x	98	3	arrange	arrange	VB
cord-016179-4i1n9j4x	98	4	the	the	DT
cord-016179-4i1n9j4x	98	5	samples	sample	NNS
cord-016179-4i1n9j4x	98	6	in	in	IN
cord-016179-4i1n9j4x	98	7	96-well	96-well	CD
cord-016179-4i1n9j4x	98	8	plate	plate	NN
cord-016179-4i1n9j4x	98	9	for	for	IN
cord-016179-4i1n9j4x	98	10	one	one	CD
cord-016179-4i1n9j4x	98	11	-	-	HYPH
cord-016179-4i1n9j4x	98	12	step	step	NN
cord-016179-4i1n9j4x	98	13	real	real	JJ
cord-016179-4i1n9j4x	98	14	-	-	HYPH
cord-016179-4i1n9j4x	98	15	time	time	NN
cord-016179-4i1n9j4x	98	16	RT	rt	NN
cord-016179-4i1n9j4x	98	17	-	-	HYPH
cord-016179-4i1n9j4x	98	18	PCR	PCR	NNP
cord-016179-4i1n9j4x	98	19	(	(	-LRB-
cord-016179-4i1n9j4x	98	20	Table	Table	NNP
cord-016179-4i1n9j4x	98	21	3	3	CD
cord-016179-4i1n9j4x	98	22	)	)	-RRB-
cord-016179-4i1n9j4x	98	23	and	and	CC
cord-016179-4i1n9j4x	98	24	set	set	VB
cord-016179-4i1n9j4x	98	25	up	up	RP
cord-016179-4i1n9j4x	98	26	the	the	DT
cord-016179-4i1n9j4x	98	27	real	real	JJ
cord-016179-4i1n9j4x	98	28	-	-	HYPH
cord-016179-4i1n9j4x	98	29	time	time	NN
cord-016179-4i1n9j4x	98	30	RT	rt	NN
cord-016179-4i1n9j4x	98	31	-	-	HYPH
cord-016179-4i1n9j4x	98	32	PCR	PCR	NNP
cord-016179-4i1n9j4x	98	33	conditions	condition	NNS
cord-016179-4i1n9j4x	98	34	.	.	.
cord-016179-4i1n9j4x	99	1	Prepare	prepare	VB
cord-016179-4i1n9j4x	99	2	nuclease	nuclease	NN
cord-016179-4i1n9j4x	99	3	-	-	HYPH
cord-016179-4i1n9j4x	99	4	free	free	JJ
cord-016179-4i1n9j4x	99	5	water	water	NN
cord-016179-4i1n9j4x	99	6	as	as	IN
cord-016179-4i1n9j4x	99	7	template	template	NN
cord-016179-4i1n9j4x	99	8	for	for	IN
cord-016179-4i1n9j4x	99	9	the	the	DT
cord-016179-4i1n9j4x	99	10	non	non	JJ
cord-016179-4i1n9j4x	99	11	-	-	JJ
cord-016179-4i1n9j4x	99	12	template	template	JJ
cord-016179-4i1n9j4x	99	13	control	control	NN
cord-016179-4i1n9j4x	99	14	 	 	_SP
cord-016179-4i1n9j4x	100	1	Samples	sample	NNS
cord-016179-4i1n9j4x	100	2	are	be	VBP
cord-016179-4i1n9j4x	100	3	arranged	arrange	VBN
cord-016179-4i1n9j4x	100	4	in	in	IN
cord-016179-4i1n9j4x	100	5	Row	row	NN
cord-016179-4i1n9j4x	100	6	A	a	NN
cord-016179-4i1n9j4x	100	7	to	to	IN
cord-016179-4i1n9j4x	100	8	H	H	NNP
cord-016179-4i1n9j4x	100	9	from	from	IN
cord-016179-4i1n9j4x	100	10	Column	column	NN
cord-016179-4i1n9j4x	100	11	1	1	CD
cord-016179-4i1n9j4x	100	12	to	to	IN
cord-016179-4i1n9j4x	100	13	12	12	CD
cord-016179-4i1n9j4x	100	14	in	in	IN
cord-016179-4i1n9j4x	100	15	one	one	CD
cord-016179-4i1n9j4x	100	16	96-well	96-well	CD
cord-016179-4i1n9j4x	100	17	plate	plate	NN
cord-016179-4i1n9j4x	100	18	.	.	.
cord-016179-4i1n9j4x	101	1	STD1	STD1	NNS
cord-016179-4i1n9j4x	101	2	to	to	IN
cord-016179-4i1n9j4x	101	3	STD8	STD8	NNP
cord-016179-4i1n9j4x	101	4	are	be	VBP
cord-016179-4i1n9j4x	101	5	tenfold	tenfold	RB
cord-016179-4i1n9j4x	101	6	serially	serially	RB
cord-016179-4i1n9j4x	101	7	diluted	dilute	VBN
cord-016179-4i1n9j4x	101	8	standard	standard	JJ
cord-016179-4i1n9j4x	101	9	RNA	RNA	NNP
cord-016179-4i1n9j4x	101	10	of	of	IN
cord-016179-4i1n9j4x	101	11	pTriEx3	pTriEx3	NNP
cord-016179-4i1n9j4x	101	12	-	-	HYPH
cord-016179-4i1n9j4x	101	13	6F/6R	6f/6r	CD
cord-016179-4i1n9j4x	101	14	from	from	IN
cord-016179-4i1n9j4x	101	15	10	10	CD
cord-016179-4i1n9j4x	101	16	2	2	CD
cord-016179-4i1n9j4x	101	17	to	to	TO
cord-016179-4i1n9j4x	101	18	10	10	CD
cord-016179-4i1n9j4x	101	19	9	9	CD
cord-016179-4i1n9j4x	101	20	copies	copy	NNS
cord-016179-4i1n9j4x	102	1	/	/	NFP
cord-016179-4i1n9j4x	103	1	μL.	μL.	NNP
cord-016179-4i1n9j4x	103	2	NTC	NTC	NNP
cord-016179-4i1n9j4x	103	3	is	be	VBZ
cord-016179-4i1n9j4x	103	4	nuclease	nuclease	NN
cord-016179-4i1n9j4x	103	5	-	-	HYPH
cord-016179-4i1n9j4x	103	6	free	free	JJ
cord-016179-4i1n9j4x	103	7	water	water	NN
cord-016179-4i1n9j4x	103	8	as	as	IN
cord-016179-4i1n9j4x	103	9	template	template	NN
cord-016179-4i1n9j4x	103	10	for	for	IN
cord-016179-4i1n9j4x	103	11	realtime	realtime	JJ
cord-016179-4i1n9j4x	103	12	RT	RT	NNP
cord-016179-4i1n9j4x	103	13	-	-	HYPH
cord-016179-4i1n9j4x	103	14	PCR	PCR	NNP
cord-016179-4i1n9j4x	103	15	(	(	-LRB-
cord-016179-4i1n9j4x	103	16	NTC	NTC	NNP
cord-016179-4i1n9j4x	103	17	)	)	-RRB-
cord-016179-4i1n9j4x	103	18	and	and	CC
cord-016179-4i1n9j4x	103	19	tenfold	tenfold	RB
cord-016179-4i1n9j4x	103	20	serially	serially	RB
cord-016179-4i1n9j4x	103	21	diluted	dilute	VBN
cord-016179-4i1n9j4x	103	22	standard	standard	JJ
cord-016179-4i1n9j4x	103	23	RNA	RNA	NNP
cord-016179-4i1n9j4x	103	24	of	of	IN
cord-016179-4i1n9j4x	103	25	pTriEx3	pTriEx3	NNP
cord-016179-4i1n9j4x	103	26	-	-	HYPH
cord-016179-4i1n9j4x	103	27	6F/6R	6f/6r	CD
cord-016179-4i1n9j4x	103	28	from	from	IN
cord-016179-4i1n9j4x	103	29	10	10	CD
cord-016179-4i1n9j4x	103	30	2	2	CD
cord-016179-4i1n9j4x	103	31	to	to	TO
cord-016179-4i1n9j4x	103	32	10	10	CD
cord-016179-4i1n9j4x	103	33	9	9	CD
cord-016179-4i1n9j4x	103	34	copies/	copies/	CD
cord-016179-4i1n9j4x	104	1	μL	μl	UH
cord-016179-4i1n9j4x	104	2	,	,	,
cord-016179-4i1n9j4x	104	3	as	as	IN
cord-016179-4i1n9j4x	104	4	STD1	STD1	NNPS
cord-016179-4i1n9j4x	104	5	to	to	IN
cord-016179-4i1n9j4x	104	6	STD8	STD8	NNP
cord-016179-4i1n9j4x	104	7	.	.	.
cord-016179-4i1n9j4x	105	1	After	after	IN
cord-016179-4i1n9j4x	105	2	the	the	DT
cord-016179-4i1n9j4x	105	3	reaction	reaction	NN
cord-016179-4i1n9j4x	105	4	complete	complete	JJ
cord-016179-4i1n9j4x	105	5	,	,	,
cord-016179-4i1n9j4x	105	6	establish	establish	VB
cord-016179-4i1n9j4x	105	7	the	the	DT
cord-016179-4i1n9j4x	105	8	standard	standard	JJ
cord-016179-4i1n9j4x	105	9	curve	curve	NN
cord-016179-4i1n9j4x	105	10	formula	formula	NN
cord-016179-4i1n9j4x	105	11	based	base	VBN
cord-016179-4i1n9j4x	105	12	on	on	IN
cord-016179-4i1n9j4x	105	13	the	the	DT
cord-016179-4i1n9j4x	105	14	Ct	ct	JJ
cord-016179-4i1n9j4x	105	15	values	value	NNS
cord-016179-4i1n9j4x	105	16	of	of	IN
cord-016179-4i1n9j4x	105	17	STD1	STD1	NNPS
cord-016179-4i1n9j4x	105	18	to	to	IN
cord-016179-4i1n9j4x	105	19	STD8	STD8	NNP
cord-016179-4i1n9j4x	105	20	.	.	.
cord-016179-4i1n9j4x	106	1	Then	then	RB
cord-016179-4i1n9j4x	106	2	,	,	,
cord-016179-4i1n9j4x	106	3	calculate	calculate	VB
cord-016179-4i1n9j4x	106	4	the	the	DT
cord-016179-4i1n9j4x	106	5	copy	copy	NN
cord-016179-4i1n9j4x	106	6	numbers	number	NNS
cord-016179-4i1n9j4x	106	7	of	of	IN
cord-016179-4i1n9j4x	106	8	the	the	DT
cord-016179-4i1n9j4x	106	9	tested	test	VBN
cord-016179-4i1n9j4x	106	10	samples	sample	NNS
cord-016179-4i1n9j4x	106	11	by	by	IN
cord-016179-4i1n9j4x	106	12	the	the	DT
cord-016179-4i1n9j4x	106	13	standard	standard	JJ
cord-016179-4i1n9j4x	106	14	curve	curve	NN
cord-016179-4i1n9j4x	106	15	formula	formula	NN
cord-016179-4i1n9j4x	106	16	.	.	.
cord-016179-4i1n9j4x	107	1	Example	example	NN
cord-016179-4i1n9j4x	107	2	for	for	IN
cord-016179-4i1n9j4x	107	3	calculating	calculate	VBG
cord-016179-4i1n9j4x	107	4	sample	sample	NN
cord-016179-4i1n9j4x	107	5	concentration	concentration	NN
cord-016179-4i1n9j4x	107	6	is	be	VBZ
cord-016179-4i1n9j4x	107	7	illustrated	illustrate	VBN
cord-016179-4i1n9j4x	107	8	in	in	IN
cord-016179-4i1n9j4x	107	9	Table	Table	NNP
cord-016179-4i1n9j4x	107	10	4	4	CD
cord-016179-4i1n9j4x	107	11	.	.	.
cord-016179-4i1n9j4x	108	1	The	the	DT
cord-016179-4i1n9j4x	108	2	standard	standard	JJ
cord-016179-4i1n9j4x	108	3	curve	curve	NN
cord-016179-4i1n9j4x	108	4	formula	formula	NN
cord-016179-4i1n9j4x	108	5	is	be	VBZ
cord-016179-4i1n9j4x	108	6	established	establish	VBN
cord-016179-4i1n9j4x	108	7	by	by	IN
cord-016179-4i1n9j4x	108	8	the	the	DT
cord-016179-4i1n9j4x	108	9	Ct	ct	JJ
cord-016179-4i1n9j4x	108	10	values	value	NNS
cord-016179-4i1n9j4x	108	11	and	and	CC
cord-016179-4i1n9j4x	108	12	the	the	DT
cord-016179-4i1n9j4x	108	13	copy	copy	NN
cord-016179-4i1n9j4x	108	14	numbers	number	NNS
cord-016179-4i1n9j4x	108	15	(	(	-LRB-
cord-016179-4i1n9j4x	108	16	Log	Log	NNP
cord-016179-4i1n9j4x	108	17	10	10	CD
cord-016179-4i1n9j4x	108	18	copies	copy	NNS
cord-016179-4i1n9j4x	108	19	/	/	SYM
cord-016179-4i1n9j4x	108	20	μL	μL	NNP
cord-016179-4i1n9j4x	108	21	)	)	-RRB-
cord-016179-4i1n9j4x	108	22	of	of	IN
cord-016179-4i1n9j4x	108	23	STD1	STD1	NNPS
cord-016179-4i1n9j4x	108	24	to	to	IN
cord-016179-4i1n9j4x	108	25	STD8	STD8	NNP
cord-016179-4i1n9j4x	108	26	:	:	:
cord-016179-4i1n9j4x	109	1	Y	y	NN
cord-016179-4i1n9j4x	109	2	=	=	SYM
cord-016179-4i1n9j4x	109	3	−3.4167X	−3.4167X	NNP
cord-016179-4i1n9j4x	109	4	+	+	CD
cord-016179-4i1n9j4x	109	5	46.917	46.917	CD
cord-016179-4i1n9j4x	110	1	(	(	-LRB-
cord-016179-4i1n9j4x	110	2	R	r	NN
cord-016179-4i1n9j4x	110	3	2	2	CD
cord-016179-4i1n9j4x	110	4	=	=	SYM
cord-016179-4i1n9j4x	110	5	0.9988	0.9988	CD
cord-016179-4i1n9j4x	110	6	)	)	-RRB-
cord-016179-4i1n9j4x	110	7	.	.	.
cord-016179-4i1n9j4x	111	1	Y	Y	NNP
cord-016179-4i1n9j4x	111	2	is	be	VBZ
cord-016179-4i1n9j4x	111	3	Ct	Ct	NNP
cord-016179-4i1n9j4x	111	4	value	value	NN
cord-016179-4i1n9j4x	111	5	acquired	acquire	VBN
cord-016179-4i1n9j4x	111	6	by	by	IN
cord-016179-4i1n9j4x	111	7	real	real	JJ
cord-016179-4i1n9j4x	111	8	-	-	HYPH
cord-016179-4i1n9j4x	111	9	time	time	NN
cord-016179-4i1n9j4x	111	10	RT	RT	NNP
cord-016179-4i1n9j4x	111	11	-	-	HYPH
cord-016179-4i1n9j4x	111	12	PCR	PCR	NNP
cord-016179-4i1n9j4x	111	13	and	and	CC
cord-016179-4i1n9j4x	112	1	X	x	NN
cord-016179-4i1n9j4x	112	2	is	be	VBZ
cord-016179-4i1n9j4x	112	3	sample	sample	NN
cord-016179-4i1n9j4x	112	4	concentration	concentration	NN
cord-016179-4i1n9j4x	112	5	presented	present	VBN
cord-016179-4i1n9j4x	112	6	by	by	IN
cord-016179-4i1n9j4x	112	7	copies/	copies/	XX
cord-016179-4i1n9j4x	113	1	μL.	μL.	NNP
cord-016179-4i1n9j4x	113	2	UDL	UDL	NNP
cord-016179-4i1n9j4x	113	3	is	be	VBZ
cord-016179-4i1n9j4x	113	4	under	under	IN
cord-016179-4i1n9j4x	113	5	detection	detection	NN
cord-016179-4i1n9j4x	113	6	limit	limit	NN
cord-016179-4i1n9j4x	113	7	Investigating	investigate	VBG
cord-016179-4i1n9j4x	113	8	turkey	turkey	NN
cord-016179-4i1n9j4x	113	9	enteric	enteric	JJ
cord-016179-4i1n9j4x	113	10	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	113	11	circulating	circulate	VBG
cord-016179-4i1n9j4x	113	12	in	in	IN
cord-016179-4i1n9j4x	113	13	the	the	DT
cord-016179-4i1n9j4x	113	14	Southeastern	Southeastern	NNP
cord-016179-4i1n9j4x	113	15	United	United	NNP
cord-016179-4i1n9j4x	113	16	States	States	NNP
cord-016179-4i1n9j4x	113	17	and	and	CC
cord-016179-4i1n9j4x	113	18	Arkansas	Arkansas	NNP
cord-016179-4i1n9j4x	113	19	during	during	IN
cord-016179-4i1n9j4x	113	20	Infection	Infection	NNP
cord-016179-4i1n9j4x	113	21	with	with	IN
cord-016179-4i1n9j4x	113	22	a	a	DT
cord-016179-4i1n9j4x	113	23	pathogenic	pathogenic	JJ
cord-016179-4i1n9j4x	113	24	turkey	turkey	NN
cord-016179-4i1n9j4x	113	25	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	113	26	isolate	isolate	VBP
cord-016179-4i1n9j4x	113	27	negatively	negatively	RB
cord-016179-4i1n9j4x	113	28	affects	affect	VBZ
cord-016179-4i1n9j4x	113	29	growth	growth	NN
cord-016179-4i1n9j4x	113	30	performance	performance	NN
cord-016179-4i1n9j4x	113	31	and	and	CC
cord-016179-4i1n9j4x	113	32	intestinal	intestinal	JJ
cord-016179-4i1n9j4x	113	33	morphology	morphology	NN
cord-016179-4i1n9j4x	113	34	of	of	IN
cord-016179-4i1n9j4x	113	35	young	young	JJ
cord-016179-4i1n9j4x	113	36	turkey	turkey	NN
cord-016179-4i1n9j4x	113	37	poults	poult	NNS
cord-016179-4i1n9j4x	113	38	in	in	IN
cord-016179-4i1n9j4x	113	39	Canada	Canada	NNP
cord-016179-4i1n9j4x	113	40	Detection	Detection	NNP
cord-016179-4i1n9j4x	113	41	of	of	IN
cord-016179-4i1n9j4x	113	42	turkey	turkey	NN
cord-016179-4i1n9j4x	113	43	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	113	44	in	in	IN
cord-016179-4i1n9j4x	113	45	commercial	commercial	JJ
cord-016179-4i1n9j4x	113	46	turkey	turkey	NN
cord-016179-4i1n9j4x	113	47	poults	poult	NNS
cord-016179-4i1n9j4x	113	48	in	in	IN
cord-016179-4i1n9j4x	113	49	Brazil	Brazil	NNP
cord-016179-4i1n9j4x	114	1	First	first	JJ
cord-016179-4i1n9j4x	114	2	full	full	JJ
cord-016179-4i1n9j4x	114	3	-	-	HYPH
cord-016179-4i1n9j4x	114	4	length	length	NN
cord-016179-4i1n9j4x	114	5	sequences	sequence	NNS
cord-016179-4i1n9j4x	114	6	of	of	IN
cord-016179-4i1n9j4x	114	7	the	the	DT
cord-016179-4i1n9j4x	114	8	S	S	NNP
cord-016179-4i1n9j4x	114	9	gene	gene	NN
cord-016179-4i1n9j4x	114	10	of	of	IN
cord-016179-4i1n9j4x	114	11	European	european	JJ
cord-016179-4i1n9j4x	114	12	isolates	isolate	NNS
cord-016179-4i1n9j4x	114	13	reveal	reveal	VBP
cord-016179-4i1n9j4x	114	14	further	further	JJ
cord-016179-4i1n9j4x	114	15	diversity	diversity	NN
cord-016179-4i1n9j4x	114	16	among	among	IN
cord-016179-4i1n9j4x	114	17	turkey	turkey	NNP
cord-016179-4i1n9j4x	114	18	coronaviruses	coronaviruses	NNPS
cord-016179-4i1n9j4x	114	19	Identification	Identification	NNP
cord-016179-4i1n9j4x	114	20	and	and	CC
cord-016179-4i1n9j4x	114	21	characterization	characterization	NN
cord-016179-4i1n9j4x	114	22	of	of	IN
cord-016179-4i1n9j4x	114	23	a	a	DT
cord-016179-4i1n9j4x	114	24	neutralizing	neutralize	VBG
cord-016179-4i1n9j4x	114	25	-	-	HYPH
cord-016179-4i1n9j4x	114	26	epitopecontaining	epitopecontaine	VBG
cord-016179-4i1n9j4x	114	27	spike	spike	NN
cord-016179-4i1n9j4x	114	28	protein	protein	NN
cord-016179-4i1n9j4x	114	29	fragment	fragment	NN
cord-016179-4i1n9j4x	114	30	in	in	IN
cord-016179-4i1n9j4x	114	31	turkey	turkey	NN
cord-016179-4i1n9j4x	114	32	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	114	33	Aromatic	aromatic	JJ
cord-016179-4i1n9j4x	114	34	amino	amino	JJ
cord-016179-4i1n9j4x	114	35	acids	acid	NNS
cord-016179-4i1n9j4x	114	36	in	in	IN
cord-016179-4i1n9j4x	114	37	the	the	DT
cord-016179-4i1n9j4x	114	38	juxtamembrane	juxtamembrane	NNP
cord-016179-4i1n9j4x	114	39	domain	domain	NN
cord-016179-4i1n9j4x	114	40	of	of	IN
cord-016179-4i1n9j4x	114	41	severe	severe	JJ
cord-016179-4i1n9j4x	114	42	acute	acute	JJ
cord-016179-4i1n9j4x	114	43	respiratory	respiratory	JJ
cord-016179-4i1n9j4x	114	44	syndrome	syndrome	NN
cord-016179-4i1n9j4x	114	45	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	114	46	spike	spike	NN
cord-016179-4i1n9j4x	114	47	glycoprotein	glycoprotein	NN
cord-016179-4i1n9j4x	114	48	are	be	VBP
cord-016179-4i1n9j4x	114	49	important	important	JJ
cord-016179-4i1n9j4x	114	50	for	for	IN
cord-016179-4i1n9j4x	114	51	receptor	receptor	NN
cord-016179-4i1n9j4x	114	52	dependent	dependent	JJ
cord-016179-4i1n9j4x	114	53	virus	virus	NN
cord-016179-4i1n9j4x	114	54	entry	entry	NN
cord-016179-4i1n9j4x	114	55	and	and	CC
cord-016179-4i1n9j4x	114	56	cell	cell	NN
cord-016179-4i1n9j4x	114	57	-	-	HYPH
cord-016179-4i1n9j4x	114	58	cell	cell	NN
cord-016179-4i1n9j4x	114	59	fusion	fusion	NN
cord-016179-4i1n9j4x	114	60	Differential	differential	JJ
cord-016179-4i1n9j4x	114	61	detection	detection	NN
cord-016179-4i1n9j4x	114	62	of	of	IN
cord-016179-4i1n9j4x	114	63	turkey	turkey	NN
cord-016179-4i1n9j4x	114	64	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	114	65	,	,	,
cord-016179-4i1n9j4x	114	66	infectious	infectious	JJ
cord-016179-4i1n9j4x	114	67	bronchitis	bronchitis	NN
cord-016179-4i1n9j4x	114	68	virus	virus	NN
cord-016179-4i1n9j4x	114	69	,	,	,
cord-016179-4i1n9j4x	114	70	and	and	CC
cord-016179-4i1n9j4x	114	71	bovine	bovine	JJ
cord-016179-4i1n9j4x	114	72	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	114	73	by	by	IN
cord-016179-4i1n9j4x	114	74	a	a	DT
cord-016179-4i1n9j4x	114	75	multiplex	multiplex	JJ
cord-016179-4i1n9j4x	114	76	polymerase	polymerase	NN
cord-016179-4i1n9j4x	114	77	chain	chain	NN
cord-016179-4i1n9j4x	114	78	reaction	reaction	NN
cord-016179-4i1n9j4x	114	79	Comparison	comparison	NN
cord-016179-4i1n9j4x	114	80	of	of	IN
cord-016179-4i1n9j4x	114	81	virus	virus	NN
cord-016179-4i1n9j4x	114	82	isolation	isolation	NN
cord-016179-4i1n9j4x	114	83	,	,	,
cord-016179-4i1n9j4x	114	84	immunohistochemistry	immunohistochemistry	NN
cord-016179-4i1n9j4x	114	85	,	,	,
cord-016179-4i1n9j4x	114	86	and	and	CC
cord-016179-4i1n9j4x	114	87	reverse	reverse	VB
cord-016179-4i1n9j4x	114	88	transcriptase	transcriptase	NN
cord-016179-4i1n9j4x	114	89	polymerase	polymerase	NN
cord-016179-4i1n9j4x	114	90	chain	chain	NN
cord-016179-4i1n9j4x	114	91	reaction	reaction	NN
cord-016179-4i1n9j4x	114	92	procedures	procedure	NNS
cord-016179-4i1n9j4x	114	93	for	for	IN
cord-016179-4i1n9j4x	114	94	detection	detection	NN
cord-016179-4i1n9j4x	114	95	of	of	IN
cord-016179-4i1n9j4x	114	96	turkey	turkey	NN
cord-016179-4i1n9j4x	114	97	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	114	98	Detection	Detection	NNP
cord-016179-4i1n9j4x	114	99	of	of	IN
cord-016179-4i1n9j4x	114	100	specific	specific	JJ
cord-016179-4i1n9j4x	114	101	polymerase	polymerase	NN
cord-016179-4i1n9j4x	114	102	chain	chain	NN
cord-016179-4i1n9j4x	114	103	reaction	reaction	NN
cord-016179-4i1n9j4x	114	104	product	product	NN
cord-016179-4i1n9j4x	114	105	by	by	IN
cord-016179-4i1n9j4x	114	106	utilizing	utilize	VBG
cord-016179-4i1n9j4x	114	107	the	the	DT
cord-016179-4i1n9j4x	114	108	50	50	CD
cord-016179-4i1n9j4x	114	109	-	-	SYM
cord-016179-4i1n9j4x	114	110	30	30	CD
cord-016179-4i1n9j4x	114	111	exonuclease	exonuclease	NN
cord-016179-4i1n9j4x	114	112	activity	activity	NN
cord-016179-4i1n9j4x	114	113	of	of	IN
cord-016179-4i1n9j4x	114	114	Thermus	Thermus	NNP
cord-016179-4i1n9j4x	114	115	aquaticus	aquaticus	NN
cord-016179-4i1n9j4x	114	116	DNA	dna	NN
cord-016179-4i1n9j4x	114	117	polymerase	polymerase	NN
cord-016179-4i1n9j4x	114	118	Specific	specific	JJ
cord-016179-4i1n9j4x	114	119	real	real	JJ
cord-016179-4i1n9j4x	114	120	time	time	NN
cord-016179-4i1n9j4x	114	121	reverse	reverse	NN
cord-016179-4i1n9j4x	114	122	transcription	transcription	NN
cord-016179-4i1n9j4x	114	123	polymerase	polymerase	NN
cord-016179-4i1n9j4x	114	124	chain	chain	NN
cord-016179-4i1n9j4x	114	125	reaction	reaction	NN
cord-016179-4i1n9j4x	114	126	for	for	IN
cord-016179-4i1n9j4x	114	127	detection	detection	NN
cord-016179-4i1n9j4x	114	128	and	and	CC
cord-016179-4i1n9j4x	114	129	quantitation	quantitation	NN
cord-016179-4i1n9j4x	114	130	of	of	IN
cord-016179-4i1n9j4x	114	131	turkey	turkey	NNP
cord-016179-4i1n9j4x	114	132	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	114	133	RNA	RNA	NNP
cord-016179-4i1n9j4x	114	134	in	in	IN
cord-016179-4i1n9j4x	114	135	tissues	tissue	NNS
cord-016179-4i1n9j4x	114	136	and	and	CC
cord-016179-4i1n9j4x	114	137	feces	fece	NNS
cord-016179-4i1n9j4x	114	138	from	from	IN
cord-016179-4i1n9j4x	114	139	turkeys	turkey	NNS
cord-016179-4i1n9j4x	114	140	infected	infect	VBN
cord-016179-4i1n9j4x	114	141	with	with	IN
cord-016179-4i1n9j4x	114	142	turkey	turkey	NN
cord-016179-4i1n9j4x	114	143	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	115	1	The	the	DT
cord-016179-4i1n9j4x	115	2	protocol	protocol	NN
cord-016179-4i1n9j4x	115	3	"	"	``
cord-016179-4i1n9j4x	115	4	Real	real	JJ
cord-016179-4i1n9j4x	115	5	-	-	HYPH
cord-016179-4i1n9j4x	115	6	time	time	NN
cord-016179-4i1n9j4x	115	7	reverse	reverse	NN
cord-016179-4i1n9j4x	115	8	transcription	transcription	NN
cord-016179-4i1n9j4x	115	9	-	-	HYPH
cord-016179-4i1n9j4x	115	10	polymerase	polymerase	NN
cord-016179-4i1n9j4x	115	11	chain	chain	NN
cord-016179-4i1n9j4x	115	12	reaction	reaction	NN
cord-016179-4i1n9j4x	115	13	for	for	IN
cord-016179-4i1n9j4x	115	14	detection	detection	NN
cord-016179-4i1n9j4x	115	15	and	and	CC
cord-016179-4i1n9j4x	115	16	quantitation	quantitation	NN
cord-016179-4i1n9j4x	115	17	of	of	IN
cord-016179-4i1n9j4x	115	18	turkey	turkey	NNP
cord-016179-4i1n9j4x	115	19	coronavirus	coronavirus	NN
cord-016179-4i1n9j4x	115	20	RNA	RNA	NNP
cord-016179-4i1n9j4x	115	21	in	in	IN
cord-016179-4i1n9j4x	115	22	feces	fece	NNS
cord-016179-4i1n9j4x	115	23	and	and	CC
cord-016179-4i1n9j4x	115	24	intestine	intestine	NN
cord-016179-4i1n9j4x	115	25	tissues	tissue	NNS
cord-016179-4i1n9j4x	115	26	"	"	''
cord-016179-4i1n9j4x	115	27	outlined	outline	VBN
cord-016179-4i1n9j4x	115	28	in	in	IN
cord-016179-4i1n9j4x	115	29	this	this	DT
cord-016179-4i1n9j4x	115	30	chapter	chapter	NN
cord-016179-4i1n9j4x	115	31	had	have	VBD
cord-016179-4i1n9j4x	115	32	been	be	VBN
cord-016179-4i1n9j4x	115	33	successfully	successfully	RB
cord-016179-4i1n9j4x	115	34	carried	carry	VBN
cord-016179-4i1n9j4x	115	35	out	out	RP
cord-016179-4i1n9j4x	115	36	in	in	IN
cord-016179-4i1n9j4x	115	37	the	the	DT
cord-016179-4i1n9j4x	115	38	authors	author	NNS
cord-016179-4i1n9j4x	115	39	'	'	POS
cord-016179-4i1n9j4x	115	40	studies	study	NNS
cord-016179-4i1n9j4x	115	41	on	on	IN
cord-016179-4i1n9j4x	115	42	molecular	molecular	JJ
cord-016179-4i1n9j4x	115	43	diagnostics	diagnostic	NNS
cord-016179-4i1n9j4x	115	44	,	,	,
cord-016179-4i1n9j4x	115	45	molecular	molecular	JJ
cord-016179-4i1n9j4x	115	46	virology	virology	NN
cord-016179-4i1n9j4x	115	47	,	,	,
cord-016179-4i1n9j4x	115	48	immunology	immunology	NN
cord-016179-4i1n9j4x	115	49	,	,	,
cord-016179-4i1n9j4x	115	50	and/or	and/or	CC
cord-016179-4i1n9j4x	115	51	vaccinology	vaccinology	NN
cord-016179-4i1n9j4x	115	52	of	of	IN
cord-016179-4i1n9j4x	115	53	turkey	turkey	NNP
cord-016179-4i1n9j4x	115	54	coronaviral	coronaviral	NNP
cord-016179-4i1n9j4x	115	55	enteritis	enteritis	NNP
cord-016179-4i1n9j4x	115	56	.	.	.
cord-016179-4i1n9j4x	116	1	Those	those	DT
cord-016179-4i1n9j4x	116	2	studies	study	NNS
cord-016179-4i1n9j4x	116	3	were	be	VBD
cord-016179-4i1n9j4x	116	4	in	in	IN
cord-016179-4i1n9j4x	116	5	part	part	NN
cord-016179-4i1n9j4x	116	6	financially	financially	RB
cord-016179-4i1n9j4x	116	7	supported	support	VBN
cord-016179-4i1n9j4x	116	8	by	by	IN
cord-016179-4i1n9j4x	116	9	USDA	USDA	NNP
cord-016179-4i1n9j4x	116	10	,	,	,
cord-016179-4i1n9j4x	116	11	North	North	NNP
cord-016179-4i1n9j4x	116	12	Carolina	Carolina	NNP
cord-016179-4i1n9j4x	116	13	Poultry	Poultry	NNP
cord-016179-4i1n9j4x	116	14	Federation	Federation	NNP
cord-016179-4i1n9j4x	116	15	,	,	,
cord-016179-4i1n9j4x	116	16	and/or	and/or	CC
cord-016179-4i1n9j4x	116	17	Indiana	Indiana	NNP
cord-016179-4i1n9j4x	116	18	Department	Department	NNP
cord-016179-4i1n9j4x	116	19	of	of	IN
cord-016179-4i1n9j4x	116	20	Agriculture	Agriculture	NNP
cord-016179-4i1n9j4x	116	21	and	and	CC
cord-016179-4i1n9j4x	116	22	technically	technically	RB
cord-016179-4i1n9j4x	116	23	assisted	assist	VBN
cord-016179-4i1n9j4x	116	24	by	by	IN
cord-016179-4i1n9j4x	116	25	Drs	Drs	NNP
cord-016179-4i1n9j4x	116	26	.	.	.
cord-016179-4i1n9j4x	117	1	Tom	Tom	NNP
cord-016179-4i1n9j4x	117	2	Brien	Brien	NNP
cord-016179-4i1n9j4x	117	3	and	and	CC
cord-016179-4i1n9j4x	117	4	David	David	NNP
cord-016179-4i1n9j4x	117	5	Hermes	Hermes	NNP
cord-016179-4i1n9j4x	117	6	,	,	,
cord-016179-4i1n9j4x	117	7	Mr.	Mr.	NNP
cord-016179-4i1n9j4x	117	8	Tom	Tom	NNP
cord-016179-4i1n9j4x	117	9	Hooper	Hooper	NNP
cord-016179-4i1n9j4x	117	10	,	,	,
cord-016179-4i1n9j4x	117	11	and	and	CC
cord-016179-4i1n9j4x	117	12	Ms.	Ms.	NNP
cord-016179-4i1n9j4x	117	13	Donna	Donna	NNP
cord-016179-4i1n9j4x	117	14	Schrader	Schrader	NNP
cord-016179-4i1n9j4x	117	15	for	for	IN
cord-016179-4i1n9j4x	117	16	clinical	clinical	JJ
cord-016179-4i1n9j4x	117	17	investigation	investigation	NN
cord-016179-4i1n9j4x	117	18	,	,	,
cord-016179-4i1n9j4x	117	19	virus	virus	NN
cord-016179-4i1n9j4x	117	20	isolation	isolation	NN
cord-016179-4i1n9j4x	117	21	and	and	CC
cord-016179-4i1n9j4x	117	22	propagation	propagation	NN
cord-016179-4i1n9j4x	117	23	,	,	,
cord-016179-4i1n9j4x	117	24	and	and	CC
cord-016179-4i1n9j4x	117	25	animal	animal	NN
cord-016179-4i1n9j4x	117	26	experimentation	experimentation	NN
cord-016179-4i1n9j4x	117	27	.	.	.