id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353703-u86ggw11	Gao, Peng	Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus	2019-11-18		.txt	text/plain	8258	412	49	We provide evidence that the virus targets the UPR central regulator GRP78 for proteasomal degradation via a mechanism that requires viral glycoprotein GP2a, while both IRE1-XBP1s and PERK-eIF2α-ATF4 signaling branches of the UPR are turned on at early stage of infection. In support of our hypothesis, antibodies to ATF4, but not the isotype control (IgG), could immunoprecipitate viral RNA from infected MARC-145 cells, as detected by RT-qPCR of the PRRSV 5'UTR region ( Fig 7D) . Because GRP78 is the master regulator, its reduced accumulation likely prevents any modulation of the UPR, keeping the ER stress pathways induced for The abundance of viral RNA was assessed by RT-PCR of ORF7, normalized against the house-keeping gene GAPDH, and then compared the benefit of the virus.	./cache/cord-353703-u86ggw11.txt	./txt/cord-353703-u86ggw11.txt