id author title date pages extension mime words sentences flesch summary cache txt cord-352664-heoj8ji8 Hubbard, Amelia Field pathogenomics reveals the emergence of a diverse wheat yellow rust population 2015-02-25 .txt text/plain 9181 460 45 In this study, we developed a robust and rapid 'field pathogenomics' strategy, using transcriptome sequencing of PST-infected wheat leaves to gain insight into the population structure of an emerging pathogen. To characterize the genotypic diversity of PST at the field level, we collected 219 samples of wheat and triticale infected with PST from 17 different counties across the UK in the spring and summer of 2013 ( Figure 1a ; Table S1 in Additional file 1). To determine the relationship between the 2013 PST field isolates and previously prevalent PST populations, the genomes of 14 UK and 7 French purified PST isolates collected between 1978 and 2011 were sequenced using an Illumina whole-genome shotgun approach Figure 2 Identification of wheat varieties using transcriptome data generated directly from PST-infected field samples. We used multivariate discriminant analysis of principal components (DAPC) with the 34,764 biallelic SNP sites to define the population structure and identify groups of genetically related PST isolates. ./cache/cord-352664-heoj8ji8.txt ./txt/cord-352664-heoj8ji8.txt