id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349341-ap5n6ijl	Kopek, Benjamin G	Three-Dimensional Analysis of a Viral RNA Replication Complex Reveals a Virus-Induced Mini-Organelle	2007-08-14		.txt	text/plain	8539	431	44	The sole FHV RNA replication factor, protein A, and FHV-specific 5-bromouridine 5'-triphosphate incorporation localized between inner and outer mitochondrial membranes inside ∼50-nm vesicles (spherules), which thus are FHV-induced compartments for viral RNA synthesis. To localize more precisely FHV RNA synthesis in relation to spherules, we incubated mitochondria isolated from uninfected and FHV-infected Drosophila cells with a nucleotide mix including 5-bromouridine 5'-triphosphate (BrUTP) and performed immunogold labeling EM with an antibody recognizing BrU incorporated into RNA, but not unincorporated BrUTP. To generate 3-D surface maps of the virus-induced membrane rearrangements associated with FHV RNA replication, we manually traced the inner and outer mitochondrial membranes (including spherules) over ;100 adjacent, 2.2nm-spaced virtual sections of selected tomographic reconstructions, and we used a computer-generated mesh overlay to join these tracings into continuous surfaces ( Figure 4 ).	./cache/cord-349341-ap5n6ijl.txt	./txt/cord-349341-ap5n6ijl.txt