id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348204-365z3qxz	Harun, Mohammad Syamsul Reza	Transcriptional profiling of feline infectious peritonitis virus infection in CRFK cells and in PBMCs from FIP diagnosed cats	2013-11-09		.txt	text/plain	4691	235	52	Real time RT-qPCR developed focusing on 2 up-regulated genes (PD-L1 and A3H) together with an apoptosis associated gene PD-1 expressions in FIPV infected CRFK cells and in PBMCs from healthy and FIP diagnosed cats produced concordant results with transcriptome data. FIPV infected cells also showed high up-regulation of PD-1 expression at 3 h.p.i. and moderately up-regulated at 12 h.p.i but were being down-regulated at 6, 9, 24 and 48 h.p.i. Meanwhile, PD-L1 gene was consistently down-regulated from 3 hours to 48 h.p.i. Peripheral Blood Mononuclear Cells (PBMCs), obtained from cats with clinical signs associated with FIP (Table 4) , were purified and analysed with real-time PCR. The reference feline genome sequence assembly of transcriptome analysis of early infection (3 h.p.i.) of CRFK cells with FIPV 79-1146 showed that the expressions of 215 transcripts (0.8% of the trimmed annotated) were statistically significant, based on Kal's Z test.	./cache/cord-348204-365z3qxz.txt	./txt/cord-348204-365z3qxz.txt