id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345863-j01l71dh	Drechsler, Yvonne	Host Gene Expression of Macrophages in Response to Feline Coronavirus Infection	2020-06-09		.txt	text/plain	5530	259	43	Considering the importance of genetics and host immune responses in viral infections, the goal of this study was to elucidate host gene expression in macrophages using RNA sequencing. Our results highlight individual host responses playing an important role, consistent with the fact that few cats develop feline infectious peritonitis despite a common presence of enteric FCoV. Cultured macrophages were infected with the FIPV 79-1146 for 2 and 17 h before RNA was collected for the expression analysis of host genes and viral reads. Viral RNA load per sample in both raw read count summation and normalized fragments per kilobase million (FPKM) average from all virus' genes combined, obtained in cellular extracts of either macrophages or CRFK cells infected with FIPV, at 2 and 17 h post-infection. In contrast to the macrophages exposed to the virus, RNA sequencing showed several log-fold increases of viral isolates in the CRFK cells used as replication controls from 2 to 17 h (Table 1 ).	./cache/cord-345863-j01l71dh.txt	./txt/cord-345863-j01l71dh.txt