id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328768-2qk884x2	Sabatier, Marina	Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses	2020-10-06		.txt	text/plain	5361	293	45	Here, we aimed to compare the sensitivity and sample and reagent contamination of three extraction methods used for viral mNGS: two automated platforms (eMAG; MagNA Pure 24, MP24) and the manual QIAamp Viral RNA Mini Kit (QIAamp). The development of metagenomics next-generation sequencing (mNGS) has enabled the exploration of whole viral nucleic acids within a clinical sample (human virome) in order to detect pathogens not targeted by conventional PCR and to identify emerging viruses [1] [2] [3] [4] [5] [6] [7] [8] . The aim of this study was to compare two automated extraction platforms commonly used in diagnostic laboratories, the eMAG (bioMérieux, Marcy-l Étoile, France) and the MagNA Pure 24 (MP24) (Roche, Basel, Switzerland), and one manual QIAamp Viral RNA Mini Kit extraction (Qiagen, Hilden, Germany), which is among one of the most popular methods used in research laboratories.	./cache/cord-328768-2qk884x2.txt	./txt/cord-328768-2qk884x2.txt