id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-324212-aqp73hi9	Wyszko, Eliza	Leadzyme formed in vivo interferes with tobacco mosaic virus infection in Nicotiana tabacum	2006-10-09		.txt	text/plain	4458	298	60	We identified a leadzyme substrate target sequence in genomic tobacco mosaic virus RNA and designed a 16‐mer oligoribonucleotide capable of forming a specific leadzyme motif with a five‐nucleotide catalytic loop. We identified a leadzyme substrate target sequence in genomic tobacco mosaic virus RNA and designed a 16-mer oligoribonucleotide capable of forming a specific leadzyme motif with a five-nucleotide catalytic loop. To achieve this goal, we analyzed the inhibition of tobacco mosaic virus (TMV) as a model system for inhibiting viral infections caused by positive single-stranded RNA (+)ssRNA viruses. In this article, we show that exogenous ssRNA with sequence complementarity binds the target site in TMV RNA to form a leadzyme motif, and in the presence of a catalytic amount of Pb 2+ , cleaves viral (+)ssRNA. Control assays, with 16-nucleotide catalytic RNA only or Pb 2+ applied in the presence of TMV, were performed to demonstrate the specificity of leadzyme cleavage.	./cache/cord-324212-aqp73hi9.txt	./txt/cord-324212-aqp73hi9.txt