id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314877-db7tze8j	Chkuaseli, Tamari	Activation of viral transcription by stepwise largescale folding of an RNA virus genome	2020-08-12		.txt	text/plain	8535	421	48	When viewed in the context of the RNA secondary structure model for the TBSV genome (48) , the DE/CE interaction corresponds to the closing stem of a sizable RNA domain, termed large domain 3 (LD3), which, along with formation of the adjacent LD2, acts to unite the AS2 and RS2 sequences ( Figure 1B) . Translational readthrough for the CIRV genome requires a long-distance RNA-RNA interaction (LDRI) between RTSL and the 3 UTR, involving the PRTE and DRTE partner sequences, respectively ( Figure 1A , B) (39) . The binding of RTSL-TL to SL59-5 was investigated functionally by introducing compensatory nucleotide substitutions into the candidate partner sequences and assessing the effects on sg mRNA1 accumulation following transfection of mutant viral RNA genomes into protoplasts pairing potential in mutants TC-6 and TC-7 diminished sg mRNA1 plus-and minus-strand levels below ∼10% of wt, while regenerating pairing capacity with alternate nucleotides in mutant TC-8 restored levels up to ∼50-62% of wt ( Figure 3B, C) .	./cache/cord-314877-db7tze8j.txt	./txt/cord-314877-db7tze8j.txt