id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-313541-fpqwzf9k	Ulloa, S.	A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit	2020-08-22		.txt	text/plain	1776	104	55	The growing demand for commercial kits used for automated extraction of SARS-CoV-2 RNA, a key step before rRT-PCR diagnosis, could cause a shortage of stocks that hinders the rapid processing of samples. SARS-CoV-2 detection by direct rRT-PCR without RNA extraction and inactivating samples at 95 °C for 5 minutes, was showed from specimens placed in UTM and molecular water, but not from samples in Hanks medium and saline buffer (Merindol et al., 2020) . Thus, the four different media used in this study (UTM, PBS 1x solution, Hanks medium and DNA/RNA Shield TM ) did not affect analytical results, because all precipitated samples were able to be detected by rRT-PCR using the whole viral panel (Orf1ab, N and S genes) and the internal control gene. Here, a simple protocol to detect SARS-CoV-2 from NPSs using rRT-PCR after a heat inactivation and a precipitation/concentration step is proposed.	./cache/cord-313541-fpqwzf9k.txt	./txt/cord-313541-fpqwzf9k.txt