id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-310192-8x37nx4s	Zhang, Huaqun	Advances that facilitate the study of large RNA structure and dynamics by nuclear magnetic resonance spectroscopy	2019-04-25		.txt	text/plain	7235	355	43	In this review, we will give a broad overview of different methods for structure determination and discuss some recent advancements in both sample preparation and data acquisition that have advanced the study of large RNAs by nuclear magnetic resonance (NMR) spectroscopy. Base-pairing, hydrogen bonding, nucleotide accessibility and other secondary structure-related characteristics, can be obtained by treating RNAs with specific chemical reagents (typically dimethyl sulfate [DMS] and/or one or more SHAPE reagents) and probing for the sites of modification by sequencing (Cordero, Kladwang, VanLang, & Das, 2012; Merino, Wilkinson, Coughlan, & Weeks, 2005; Tian & Das, 2016; Weeks, 2010) . Traditionally, these studies have been applied to relatively small RNAs. The ability to site-specifically incorporate 13 C labels within an RNA ribose and/or base makes NMR spectroscopy a powerful tool for dynamics studies of large RNAs, expanding the types of experiments that can be conducted and simplifying analysis (see data acquisition and analysis advancements below).	./cache/cord-310192-8x37nx4s.txt	./txt/cord-310192-8x37nx4s.txt