id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307603-uqr6r14u	Kauppinen, S.	Locked Nucleic Acid: High-Affinity Targeting of Complementary RNA for RNomics	2006		.txt	text/plain	5782	298	47	Several studies have demonstrated that LNA-modified oligonucleotides exhibit unprecedented thermal stability when hybridized with their DNA and RNA target molecules (Koshkin et al. 2005 ); (2) easy access-LNAs (fully modified or mixmers) are commercially available; (3) high-affinity and sequence-selective targeting of RNA molecules in vitro or in vivo Koshkin et al. Recently, it has been demonstrated that LNAzymes containing 3-4 LNA monomers at the ends of the binding arms cleave viral RNA structures that are resistant to hydrolysis by the corresponding unmodified DNAzyme, i.e., that efficient cleavage is correlated with improved binding affinity towards the target (Schubert et al. Efficient selection of polyadenylated mRNA from eukaryotic cells and tissues is an essential step for a wide selection of functional genomics applications, including full-length complementary (c)DNA library construction and sequencing, Northern and dot blot analyses, gene expression profiling by microarrays, and quantitative RT-PCR. LNA (locked nucleic acid): high affinity targeting of complementary RNA and DNA	./cache/cord-307603-uqr6r14u.txt	./txt/cord-307603-uqr6r14u.txt