id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303408-coesfldm	Konstantinova, Pavlina	Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome	2007-03-01		.txt	text/plain	4886	277	53	BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) can be inhibited by means of RNA silencing or interference (RNAi) using synthetic short interfering RNAs (siRNAs) or gene constructs encoding short hairpin RNAs (shRNAs) or long hairpin RNAs (lhRNAs). Although these escape variants lost the ability to trans-inhibit HIV-1, they effectively outgrew the wild-type virus in competition experiments in SupT1 cells. In the current study, we constructed the HIV-lhNef variant, which contains a 300 bp extended hairpin structure at the 3' genome position of the Nef gene of the otherwise wild-type HIV-1. HEK293T cells were transfected with the wild type HIV-1 construct or HIV-lhNef. We measured CA-p24 in the culture supernatant as a measure of virus production 2 days post-transfection. Compared to the effective HIV-lhNef inhibitor, all AS escape variants had lost the ability to actively inhibit wild-type virus production (Fig. 4B ).	./cache/cord-303408-coesfldm.txt	./txt/cord-303408-coesfldm.txt