id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302830-5psqxxc8	Ávila‐Pérez, Ginés	Ultrastructural characterization of membranous torovirus replication factories	2016-07-05		.txt	text/plain	9037	426	51	To characterize the torovirus RTCs, we generated specific sera for BEV M pro , Hel and RdRp, three key proteins in the formation and functioning of nidovirus RTCs, that were used in confocal and transmission electron microscopy (TEM) assays to analyse the location of viral components involved in BEV replication. It has been shown that in cells infected with different nidoviruses, virus-induced membrane reorganization produces characteristic DMVs, as well as other membranous structures, which have been related with the viral RNA replication and transcription processes. To investigate whether torovirus DMVs are the sites where replication and transcription occur, we obtained specific sera for the BEV M pro , Hel and RdRp, three key proteins in the formation and functioning of RTCs. All showed a high colocalization with each other and with newly synthesized RNAs. However, the dsRNA, widely used as a marker of RTC in several positive-strand RNA viruses, only showed a partial colocalization with the nsps.	./cache/cord-302830-5psqxxc8.txt	./txt/cord-302830-5psqxxc8.txt