id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302368-uhhtvdif	Longhini, Andrew P.	Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations	2016-04-07		.txt	text/plain	6542	323	49	Finally, we showcase the improvement in spectral quality arising from reduced crowding and narrowed linewidths, and accurate analysis of NMR relaxation dispersion (CPMG) and TROSY-based CEST experiments to measure μs-ms time scale motions, and an improved NOESY strategy for resonance assignment. Additionally, we show that the measurements of relaxation parameters using CPMG, R 1 , and CEST are possible for both small and large RNAs. Furthermore, we demonstrate substantial improvements in signalto-noise and line width for relaxation optimized spectroscopy (TROSY) experiments compared to the traditional heteronuclear single quantum coherence (HSQC) exNucleic Acids Research, 2016, Vol. 44, No. 6 e52 periments for isolated two-spin systems approximated by our purine and pyrimidine labeling schemes (30) (31) (66) (67) . Thus, RNAs synthesized with our selective site-specifically labeled NTPs should benefit from TROSY based NMR experiments that reduce the problems of crowding, fast signal decay, low resolution, and decreased S/N ratios (12, 34, 31, (66) (67) (80) (81) .	./cache/cord-302368-uhhtvdif.txt	./txt/cord-302368-uhhtvdif.txt