id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301226-hmc2wmst	Randazzo, Walter	Metropolitan Wastewater Analysis for COVID-19 Epidemiological Surveillance	2020-04-27		.txt	text/plain	2158	127	52	Methods: Here, we have used RT-qPCR for SARS-CoV-2 detection in a series of longitudinal metropolitan wastewaters samples collected during the earliest stages of the epidemic in the Region of Valencia, Spain. Here, we show that SARS-CoV-2 can be reproducibly detected by RT-qPCR in longitudinal samples from sewage treatment plants that receive wastewaters from over one million inhabitants in the metropolitan area of Valencia, Spain. Following concentration of viral content by flocculation, a standard RT-qPCR procedure allowed us to detect SARS-CoV-2 RNA in 12/12 samples collected from March 9 to April 14, 2020, with Ct values ranging between 34•00 and 37•84, correspondingly revealing between 5•22 and 5•99 log 10 genomic copies (gc)/L (Table 1) . Interestingly, we consistently detected SARS-CoV-2 RNA in samples collected on March 9 and March 11, when only 50 and 76 cumulative cases were declared in the entire Region of Valencia.	./cache/cord-301226-hmc2wmst.txt	./txt/cord-301226-hmc2wmst.txt