id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-297880-jlnv90vn	Stewart, Hazel	Transcriptional and Translational Landscape of Equine Torovirus	2018-08-16		.txt	text/plain	10291	491	49	Using the prototype species equine torovirus (EToV), we performed parallel RNA sequencing (RNA-seq) and ribosome profiling (Ribo-seq) to analyze the relative expression levels of the known torovirus proteins and transcripts, chimeric sequences produced via discontinuous RNA synthesis (a characteristic of the nidovirus replication cycle), and changes in host transcription and translation as a result of EToV infection. The genomes of members of the order Nidovirales are positive-sense, polycistronic RNAs. One of the hallmarks of this virus order is the utilization of an unusual transcription mechanism to express the genes encoding structural and accessory proteins, which reside downstream of the large replicase open reading frames (ORFs) 1a and 1b (Fig. 1) . RNA sequencing (RNA-seq) confirmed previous reports that EToV utilizes a unique combination of both discontinuous and nondiscontinuous RNA synthesis to generate its repertoire of sgRNAs. Strikingly, we also identified a small proportion of chimeric transcripts spanning from the leader to the body TRS of the N protein gene, indicating that discontinuous and nondiscontinuous mechanisms compete in this location.	./cache/cord-297880-jlnv90vn.txt	./txt/cord-297880-jlnv90vn.txt