id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294483-mozabpcs	Choudhary, Manohar Lal	Development of in vitro transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India	2020-04-28		.txt	text/plain	637	50	66	Ten-fold serial dilutions of each transcribed RNA products were tested with respective gene primer probe sets for specific detection and limit of detection. Further, the IVT RNA of each gene was serially diluted 10-fold (10 1 to 10 10 ), and the performance was tested with genespecific primer probe by real-time RT-PCR. When the assay was first set up at the National Influenza Centre of ICMR-NIV, Pune, the IVT RNA for E and SARS coronavirus Frankfurt 1 strain were received from EVAg. The real-time PCR screening assay (E gene) was also established at the 13 VRDLs as part of ICMR's efforts to expand testing to VRDLs closer to major airports 3 . This necessitated the development of an indigenous IVT RNA for E and RdRp. In addition, majority of the WHO screening protocols (5 of 6) are based on N gene targeting different nucleotide positions and require multiple specific positive controls 4 .	./cache/cord-294483-mozabpcs.txt	./txt/cord-294483-mozabpcs.txt