id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293215-6flf5ig0	Eriksson, Klara Kristin	Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs	2007-11-28		.txt	text/plain	4682	325	58	title: Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs Based on cloned coronaviral cDNA, we describe the generation of recombinant coronaviruses and stable cell lines containing coronaviral replicon RNAs. Initially, the vaccinia virus-based reverse genetic system was established for the generation of recombinant human coronavirus 229E. This section describes the preparation of purified vaccinia virus DNA that can be used: (i) for the in vitro ligation with the assembled coronavirus full-length cDNA (see Section 3.1.4), and (ii) as template for in vitro transcription reactions (see Section 3.3.1). First, a fulllength coronavirus RNA is produced using the genomic DNA of a vaccinia virus containing the full-length coronavirus cDNA insert as a template for in vitro transcription. 1. Based on a full-length coronavirus cDNA cloned in vaccinia virus, a replicon RNA-encoding cDNA can be generated using vaccinia virus-mediated homologous recombination as described in Section 3.2.	./cache/cord-293215-6flf5ig0.txt	./txt/cord-293215-6flf5ig0.txt