id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290550-u8x9drva	Radford, Alan D.	Application of next-generation sequencing technologies in virology	2012-09-17		.txt	text/plain	7931	382	43	Following on from electron microscopy, cell culture and PCR, next-generation sequencing is one of these methodologies that is now changing the way that we understand viruses, particularly in the areas of genome sequencing, evolution, ecology, discovery and transcriptomics. In addition, improved methods for analysis will become available, opening yet further applications in virology including routine diagnostic work on individuals, and new understanding of the interaction between viral and host transcriptomes. Although this review has concentrated on the use of NGS to study viral sequences directly, it is clear that these approaches provide new opportunities to explore the interaction of replicating viruses with their host, particularly their transcriptome, in a non-targeted, hypothesis-generating mode. Viral transcriptome analysis of feline immunodeficiency virus infected cells using second generation sequencing technology	./cache/cord-290550-u8x9drva.txt	./txt/cord-290550-u8x9drva.txt