id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-288651-bgo8istm	SHI, Yi	Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs	2005-03-17		.txt	text/plain	3062	242	62	RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Specific inhibition of cellular mRNA by RNAi can be triggered in mammalian cells by the introduction of synthetic 21-to 23-nucleotide duplexes of RNA N genes of SARS-CoV and evaluated their effects on viral genes expression in Vero E6 cells. The results show that all siRNA duplexes specifically reduced SARS-CoV genes expression to different extents compared with the control (Fig. 1) . Kinetic study results (Fig. 2B ) revealed a continuous increase in the specific inhibition of SARS-CoV genes expression by No. 5, No. 6, and No. 16 siRNA from 24 to 72 h after transfection.	./cache/cord-288651-bgo8istm.txt	./txt/cord-288651-bgo8istm.txt