id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-287018-g4y5kjju	Konstantinova, P	Inhibition of human immunodeficiency virus type 1 by RNA interference using long-hairpin RNA	2006-05-18		.txt	text/plain	7164	454	56	In order to solve this durability problem, we tested DNA constructs encoding virus-specific long-hairpin RNAs (lhRNAs) for their ability to inhibit HIV-1 production. The lhRNA constructs were compared with in vitro diced double-stranded RNA and a DNA construct encoding an effective nef-specific shRNA for their ability to inhibit HIV-1 production in cells. For this reason, the DNA constructs encoding lhRNAs (a single-hairpin molecule) and long dsRNAs (two complementary molecules that form a duplex) were designed to target tat, rev and nef sequences as indicated in Figure 1 . 21 nef2 dsRNA induced a decrease in luciferase expression, but an even more pronounced level of inhibition was Figure 1 Scheme of the human immunodeficiency virus type 1 (HIV-1) pLAI proviral genome and target sequences used for the design of long-hairpin RNAs (lhRNAs).	./cache/cord-287018-g4y5kjju.txt	./txt/cord-287018-g4y5kjju.txt