id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286332-cdg4im5h	van Beurden, Steven J.	A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination	2017-06-12		.txt	text/plain	6995	350	54	Herein, the genomic part coding for the spike glycoprotein ectodomain was replaced by that of the coronavirus mouse hepatitis virus (MHV), allowing for the selection and propagation of recombinant mIBV in murine cells. This problem was solved by transfecting IBV genomic RNA into otherwise non-susceptible cells, exchanging the IBV spike gene by that of the mouse hepatitis virus (MHV) provided as part of a synthetic RNA, and by subsequently rescuing recombinant IBV from infected/ transfected cells in embryonated eggs (Fig. 1) . b Stage 1 in targeted RNA recombination: an interspecies chimeric murinized IBV with a MHV spike ectodomain (mIBV) is generated by a single recombination event of IBV genomic RNA with synthetic RNA transcribed from donor plasmid p-mIBV in the 3′-end region of the 1b gene (indicated by a black curved line). Upon transfection of synthetic rIBV donor RNA into mIBV-infected murine LR7 cells, subsequent infectious IBV virus particles could be rescued in ECE.	./cache/cord-286332-cdg4im5h.txt	./txt/cord-286332-cdg4im5h.txt