id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281174-3c1vue0y	Greene, Shermalyn R	Evaluation of the NucliSens® Basic Kit assay for detection of Norwalk virus RNA in stool specimens	2003-01-16		.txt	text/plain	5250	248	48	The application of a rapid nucleic acid sequence-based amplification (NASBA) assay for the detection of NLV RNA in stool is described using the NucliSens® Basic Kit. Primers and probes for the NLV Basic Kit assay were based on the RNA polymerase region of the prototype NLV, Norwalk virus (NV) genome and could consistently detect 10(4) RT-PCR detectable units of NV RNA in a stool filtrate. Despite the specificity of the NASBA primer/probe sequences for NV, other representatives from both NLV genogroups I and II could be detected by the Basic Kit assay in outbreak stool specimens, although the results were inconsistent. In this study, we report the application of a rapid NASBA assay for the detection of NV RNA in stool using the NucliSens † Basic Kit. This assay was compared directly with RT-PCR and used for the detection of NV and other NLVs in fecal specimens obtained from human challenge studies and NLV outbreaks.	./cache/cord-281174-3c1vue0y.txt	./txt/cord-281174-3c1vue0y.txt