id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273609-whm2ce4u	Li, Qingdi Quentin	Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment	2012-06-29		.txt	text/plain	8166	373	47	title: Evaluation of reference genes for real-time quantitative PCR studies in Candida glabrata following azole treatment BACKGROUND: The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The most commonly used reference genes, including β-actin, cyclophilin, GAPDH, tubulin, and 18S and 28S ribosomal RNAs, have shown variable expression levels in different cells and tissues under different conditions, and therefore they are unsuitable for normalization purposes owing to large measurement error [6, . As seen in Table 7 , normalization of the RT-qPCR data against the reference genes suggested as optimal by the four software packages (hkgFinder, geNorm, BestKeeper, and NormFinder) or the 2 -ΔΔCT method, gave comparable relative expression levels of the target genes under fluconazole treatment in C.	./cache/cord-273609-whm2ce4u.txt	./txt/cord-273609-whm2ce4u.txt