id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273487-nfgjz6f9	Xu, Zaikun	The helicase activity of DDX56 is required for its role in assembly of infectious West Nile virus particles	2012-11-10		.txt	text/plain	5354	286	56	Loss of DDX56 helicase activity did not affect expression of WNV capsid protein (Fig. 4A ) nor its secretion from infected cells in the form of virus particles (Fig. 5A ). Data in Fig. 5B show that virus particles isolated from infected cells expressing helicase dead mutants D166N and E167Q contained 3-4 times less genomic RNA than those isolated from non-silencing cells or cells expressing RNAi-resistant wild type DDX56. Data in Fig. 6 show that in transfected or infected cells, the WNV capsid does not bind to the DEAD box helicase-containing region of DDX56 (DDX56-NT-myc), but rather, the C-terminal part of the protein, which was produced in cells expressing DDX56-CT-myc. The helicase activity of DDX56 is not essential for replication or assembly of WNV virions per se but our data indicate that it is critical for infectivity of virus particles.	./cache/cord-273487-nfgjz6f9.txt	./txt/cord-273487-nfgjz6f9.txt