id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-272702-7uc4ozjy	Graham, T. G. W.	Inexpensive, versatile and open-source methods for SARS-CoV-2 detection	2020-09-18		.txt	text/plain	8061	483	59	We therefore tested whether we could detect SARS-CoV-2 RNA by adding 1 μ l of each swab sample to 20 μ l TaqPath reactions containing the N1, N2, and RNase P (RP) probes ( Fig 2A) . Taken together, these results show that RT-qPCR with BEARmix can detect SARS-CoV-2 in clinical samples, either using purified RNA or by direct addition of swab samples, albeit with somewhat less sensitivity than commercial TaqPath master mix. To evaluate a complete protocol in which swab samples are collected into PK solution and then added directly to BEARmix RT-PCRs, we prepared contrived swab samples in which live virus was mixed with pathogenfree human nasal fluid prior to dilution into either DNA/RNA Shield, VCM containing 0.4 mg/ml proteinase K, or a solution of 0.4 mg/ml proteinase K in water (Fig 6) . Here we have developed simple, academic laboratory-derived methods for RNA extraction, direct sample addition, and RT-PCR detection that provide low-cost alternatives to the use of commercial kits (Fig 8) .	./cache/cord-272702-7uc4ozjy.txt	./txt/cord-272702-7uc4ozjy.txt