id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-271434-30nh2gc7	Tian, Fei	A fully automated centrifugal microfluidic system for sample-to-answer viral nucleic acid testing	2020-07-27		.txt	text/plain	4323	209	49	In this work, we develop an automated centrifugal microfluidic system (Figure 1 ) consisting of a microfluidic disc and a customized instrument for rapid sample-to-answer detection of SARS-CoV-2 armored RNA particles with high sensitivity and specificity. Virus lysis buffer, RT-LAMP reagents, and mineral oil were sequentially injected into the microfluidic disc for on-chip release of viral nucleic acids, amplification of target RNA, and sealing of reaction unit. To demonstrate the automated detection of viral nucleic acids by the centrifugal microfluidic system, we loaded different concentrations of SARS-CoV-2 armored RNA particles with N gene (0.5, 1, 10, 10 2 , 10 3 copies/μL) into five reaction units of the microfluidic disc, and used the instrument to carry out on-chip release of viral nuclei acids, RT-LAMP, and realtime fluorescence signal detection.	./cache/cord-271434-30nh2gc7.txt	./txt/cord-271434-30nh2gc7.txt