id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-269294-vx7xr80t	Kwong, Ann D.	Viral and cellular RNA helicases as antiviral targets	2005-09-23		.txt	text/plain	6378	398	55	Here, we discuss the role of these enzymes, factors affecting their potential as drug targets and progress in the development of agents that inhibit their activity using the hepatitis C virus-encoded helicase NS3 and the cellular helicase DDX3 adopted for use by HIV-1 as examples. The solution of the crystal structure of HCV helicase complexed with oligonucleotide, as well as mutagenesis studies, have identified key residues that are essential for enzyme activity or translocation of the RNA substrate 13, 72 . Theoretically, an unwinding assay should increase the odds of finding an inhibitor because inhibition of any of the potential mechanisms of action listed above, except those requiring a cellular environment (for example, turnover and replicase-complex formation), should result in 'hits' (for example, low-potency chemical starting points for medicinal chemistry optimization). Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding	./cache/cord-269294-vx7xr80t.txt	./txt/cord-269294-vx7xr80t.txt