id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266634-bww62vx8	Gopinath, M.	Evidence for N(7) guanine methyl transferase activity encoded within the modular domain of RNA-dependent RNA polymerase L of a Morbillivirus	2015-10-07		.txt	text/plain	2304	104	53	In the present work, using partially purified recombinant RNA polymerase complex of rinderpest virus expressed in insect cells, we demonstrate the in vitro methylation of capped mRNA. Considering the presence of both KDKE tetrad, responsible for 2 0 -O-methyl transferase as well as GXGXG motif for SAM substrate binding, domain III could likely represent the methyl transfer module (both N 7 -guanine and 2 0 -Omethyl) of RPV L protein. Consensus sequence between the viral mRNAs is given in bold the capped RNA with the partially purified L-P complex from insect cells resulted in a concentration dependent N 7 guanine methylation of 6 bp substrate (Fig. 2b , marked as rL) which was not detected in a mock-purified high-density fraction from insect cells infected with non-recombinant baculovirus (Fig. 2b, mock) . Further, to functionally validate the methyl transferase activity of domain III (aa 1717-2183, LD3) of RPV L protein, LD3 was purified from insect cells using metal chelate affinity chromatography as described earlier [8] .	./cache/cord-266634-bww62vx8.txt	./txt/cord-266634-bww62vx8.txt