id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-265381-ppjohov8	Pillai-Nair, Neeta	Cis-acting Regulatory Elements in the Potato Virus X 3′ Non-translated Region Differentially Affect Minus-strand and Plus-strand RNA Accumulation	2003-02-21		.txt	text/plain	10016	521	57	6 -13 Cis-acting elements required for minus-strand RNA accumulation in vivo or RNA synthesis in vitro have been well studied for the plant plus-strand RNA viruses that contain tRNA-like structures in the 3 0 NTR 2 such as brome mosaic virus (BMV), 14 -16 cucumber mosaic virus (CMV), 17 tobacco mosaic virus (TMV) 18 and turnip yellow mosaic virus (TYMV), 19, 20 and for several other viruses such as alfalfa mosaic virus (AlMV), 21, 22 barley stripe mosaic virus (BSMV), 23 cymbidium ringspot virus (CymRSV), 24 red clover necrotic mosaic virus (RCNMV) 25 and turnip crinkle virus (TCV). The role of the 3 0 NTR cis-acting sequences and/ or structures in PVX RNA accumulation was studied by solution structure probing and by introducing wild-type (w.t.) and mutant transcripts into tobacco protoplasts for analysis of minus and plusstrand RNA accumulation by S 1 nuclease protection assays.	./cache/cord-265381-ppjohov8.txt	./txt/cord-265381-ppjohov8.txt